| |||||||||||||||
| These NOD.AI4alpha Tg mice express a rearranged T cell receptor (TCR) alpha chain isolated from a Kd (MHC Class I)-restricted pancreatic beta cell-autoreactive CD8+ T cell clone (designated AI4 ). On the NOD/ShiLt background, female mice expressing the transgene exhibit a slightly, but not significantly, accelerated development of insulin dependent diabetes mellitus (IDDM). | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names NOD/Lt-Tg(TcraAI4)1Dvs (Changed: 23-FEB-07 ) Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse H2 Haplotype g7 Generation N6F28
Generation DefinitionsAppearance
albino, pink eyed
Related Genotype: A/A Tyrc/TyrcDescription
These NOD.AI4alpha Tg mice express a rearranged T cell receptor (TCR) alpha chain isolated from a Kd (MHC Class I)-restricted pancreatic beta cell-autoreactive CD8+ T cell clone (designated AI4 ). On the NOD/ShiLt background, female mice expressing the transgene exhibit a slightly, but not significantly, accelerated development of insulin dependent diabetes mellitus (IDDM). In combination with NOD/ShiLt-Tg(TcrbAI4)1Dvs (Stock No. 004335) the AI4 clonotype accelerates development of IDDM. These mice may be useful for studying CD8 T cells and IDDM development.Development
The transgene encoding the TCR alpha chain from clone AI4 was injected into NOD/Lt zygotes. The strain has been maintained by sibling mating carriers. (Graser et al., 2000.)
Related Strains
Strains carrying Tg(TcraAI4)1Dvs allele
004554 B6.NOD-(D17Mit21-D17Mit10) Tg(TCRaAI4)1Dvs/DvsJ 006436 NOD.Cg-(Gpi1-D7Mit346)C57BL/6J Tg(TcraAI4)1Dvs/DvsJ 004347 NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs/DvsJ View Strains carrying Tg(TcraAI4)1Dvs (3 strains)
Phenotype
Phenotype Information
- Characteristics of this human disease are associated with transgenes and other mutation types in the mouse.
Diabetes Mellitus, Insulin-Dependent; IDDM
assigned by genotype
Tg(TcraAI4)1Dvs/0
NOD-Tg(TcraAI4)1Dvs
- hematopoietic system phenotype
- abnormal CD4-positive T cell morphology
- T cells expressing the transgene comprise ~30% of the total peripheral blood lymphocyte population, a significantly higher fraction of these T cells resides in the CD4 lineage rather than in the expected CD8 lineage (MGI Ref ID J:93553)
- immune system phenotype
- abnormal CD4-positive T cell morphology
- T cells expressing the transgene comprise ~30% of the total peripheral blood lymphocyte population, a significantly higher fraction of these T cells resides in the CD4 lineage rather than in the expected CD8 lineage (MGI Ref ID J:93553)
- increased susceptibility to autoimmune diabetes
- female NOD mice carrying this transgene develop IDDM at a slightly but not significantly accelerated rate compared to nontransgenic NOD controls (MGI Ref ID J:93553)
This mouse can be used to support research in many areas including:
Immunology, Inflammation and Autoimmunity Research
Autoimmunity
Research Tools
Immunology and Inflammation Research
T Cell Receptor Transgenics
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(TcraAI4)1Dvs | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, David V Serreze | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | AI4aTg; | ||
| Mutation Made By | Dr. David Serreze, The Jackson Laboratory | ||
| Strain of Origin | NOD/ShiLt | ||
| Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory | ||
| Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory | ||
| General Note |
In conjunction with Rag1tm1Mom
| ||
| Molecular Note | The rearranged Tcra transgene expressed in these mice is derived from the pancreatic beta cell-reactive CD8 T lymphocyte clone AI4. The transgene contains a Tcra-V8/Tcra-J cDNA segment. [MGI Ref ID J:93553] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(TcraA14), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Tg(TcraAI4)1Dvs relatedChen J; Gusdon AM; Piganelli J; Leiter EH; Mathews CE. 2011. mt-Nd2(a) Modifies resistance against autoimmune type 1 diabetes in NOD mice at the level of the pancreatic beta-cell. Diabetes 60(1):355-9. [PubMed: 20980458] [MGI Ref ID J:170153]
Chen YG; Chen J; Osborne MA; Chapman HD; Besra GS; Porcelli SA; Leiter EH; Wilson SB; Serreze DV. 2006. CD38 is required for the peripheral survival of immunotolerogenic CD4+ invariant NK T cells in nonobese diabetic mice. J Immunol 177(5):2939-47. [PubMed: 16920929] [MGI Ref ID J:112615]
Chen YG; Silveira PA; Osborne MA; Chapman HD; Serreze DV. 2007. Cellular expression requirements for inhibition of type 1 diabetes by a dominantly protective major histocompatibility complex haplotype. Diabetes 56(2):424-30. [PubMed: 17259387] [MGI Ref ID J:121957]
Choisy-Rossi CM; Holl TM; Pierce MA; Chapman HD; Serreze DV. 2004. Enhanced pathogenicity of diabetogenic T cells escaping a non-MHC gene-controlled near death experience. J Immunol 173(6):3791-800. [PubMed: 15356126] [MGI Ref ID J:167508]
DiLorenzo TP; Lieberman SM; Takaki T; Honda S; Chapman HD; Santamaria P; Serreze DV; Nathenson SG. 2002. During the early prediabetic period in NOD mice, the pathogenic CD8(+) T-cell population comprises multiple antigenic specificities. Clin Immunol 105(3):332-41. [PubMed: 12498815] [MGI Ref ID J:94192]
Driver JP; Chen YG; Zhang W; Asrat S; Serreze DV. 2011. Unmasking genes in a type 1 diabetes-resistant mouse strain that enhances pathogenic CD8 T-cell responses. Diabetes 60(4):1354-9. [PubMed: 21307079] [MGI Ref ID J:171763]
Driver JP; Scheuplein F; Chen YG; Grier AE; Wilson SB; Serreze DV. 2010. Invariant natural killer T-cell control of type 1 diabetes: a dendritic cell genetic decision of a silver bullet or Russian roulette. Diabetes 59(2):423-32. [PubMed: 19903740] [MGI Ref ID J:164162]
Graser RT; DiLorenzo TP; Wang F; Christianson GJ; Chapman HD; Roopenian DC; Nathenson SG; Serreze DV. 2000. Identification of a CD8 T cell that can independently mediate autoimmune diabetes development in the complete absence of CD4 T cell helper functions. J Immunol 164(7):3913-8. [PubMed: 10725754] [MGI Ref ID J:93553]
Lee JS; Scandiuzzi L; Ray A; Wei J; Hofmeyer KA; Abadi YM; Loke P; Lin J; Yuan J; Serreze DV; Allison JP; Zang X. 2012. B7x in the periphery abrogates pancreas-specific damage mediated by self-reactive CD8 T cells. J Immunol 189(8):4165-74. [PubMed: 22972920] [MGI Ref ID J:190522]
Leiter EH; Reifsnyder P; Driver J; Kamdar S; Choisy-Rossi C; Serreze DV; Hara M; Chervonsky A. 2007. Unexpected functional consequences of xenogeneic transgene expression in beta-cells of NOD mice. Diabetes Obes Metab 9 Suppl 2:14-22. [PubMed: 17919174] [MGI Ref ID J:127015]
Pang S; Zhang L; Wang H; Yi Z; Li L; Gao L; Zhao J; Tisch R; Katz JD; Wang B. 2009. CD8(+) T cells specific for beta cells encounter their cognate antigens in the islets of NOD mice. Eur J Immunol 39(10):2716-24. [PubMed: 19658094] [MGI Ref ID J:153282]
Samanta D; Mukherjee G; Ramagopal UA; Chaparro RJ; Nathenson SG; DiLorenzo TP; Almo SC. 2011. Structural and functional characterization of a single-chain peptide-MHC molecule that modulates both naive and activated CD8+ T cells. Proc Natl Acad Sci U S A 108(33):13682-7. [PubMed: 21825122] [MGI Ref ID J:175993]
Serreze DV; Choisy-Rossi CM; Grier AE; Holl TM; Chapman HD; Gahagan JR; Osborne MA; Zhang W; King BL; Brown A; Roopenian D; Marron MP. 2008. Through Regulation of TCR Expression Levels, an Idd7 Region Gene(s) Interactively Contributes to the Impaired Thymic Deletion of Autoreactive Diabetogenic CD8+ T Cells in Nonobese Diabetic Mice. J Immunol 180(5):3250-9. [PubMed: 18292549] [MGI Ref ID J:131557]
Stolp J; Chen YG; Cox SL; Henck V; Zhang W; Tsaih SW; Chapman H; Stearns T; Serreze DV; Silveira PA. 2012. Subcongenic analyses reveal complex interactions between distal chromosome 4 genes controlling diabetogenic B cells and CD4 T cells in nonobese diabetic mice. J Immunol 189(3):1406-17. [PubMed: 22732593] [MGI Ref ID J:189784]
Thayer TC; Delano M; Liu C; Chen J; Padgett LE; Tse HM; Annamali M; Piganelli JD; Moldawer LL; Mathews CE. 2011. Superoxide production by macrophages and T cells is critical for the induction of autoreactivity and type 1 diabetes. Diabetes 60(8):2144-51. [PubMed: 21715554] [MGI Ref ID J:186804]
Tsui H; Chan Y; Tang L; Winer S; Cheung RK; Paltser G; Selvanantham T; Elford AR; Ellis JR; Becker DJ; Ohashi PS; Dosch HM. 2008. Targeting of pancreatic glia in type 1 diabetes. Diabetes 57(4):918-28. [PubMed: 18198358] [MGI Ref ID J:135214]
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $.00 Female or Male Hemizygous or Homozygous for Tg(TcraAI4)1Dvs - Tg/?
Price per Pair (US dollars $) Pair Genotype $.00 Hemizygous or Homozygous for Tg(TcraAI4)1Dvs - Tg/? x Hemizygous or Homozygous for Tg(TcraAI4)1Dvs - Tg/? Standard Supply
Research Strain. Availability determined by The Jackson Laboratory scientist holding the strain.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $.00 Female or Male Hemizygous or Homozygous for Tg(TcraAI4)1Dvs - Tg/?
Price per Pair (US dollars $) Pair Genotype $.00 Hemizygous or Homozygous for Tg(TcraAI4)1Dvs - Tg/? x Hemizygous or Homozygous for Tg(TcraAI4)1Dvs - Tg/? Standard Supply
Research Strain. Availability determined by The Jackson Laboratory scientist holding the strain.
|
|
|
Standard Supply
Research Strain. Availability determined by The Jackson Laboratory scientist holding the strain.
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.