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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names NOD/Lt-Tg(TcrbAI4)1Dvs (Changed: 23-FEB-07 ) Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse H2 Haplotype g7 Generation N2F29 (18-DEC-06) Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/TyrcDevelopment
The transgene encoding the TCR beta chain from clone AI4 was injected into NOD/Lt zygotes. The colony has been maintained by sibling breeding carriers. (Graser et al., 2000.)
Related Strains
Strains carrying Tg(TcrbAI4)1Dvs allele
View Strains carrying Tg(TcrbAI4)1Dvs (4 strains)
Phenotype
Phenotype Information
NOT Diabetes Mellitus, Insulin-Dependent; IDDM - No similarity to the expected human disease phenotype was found.4
4 One or more human genes are associated with this human disease. The mouse genotype may involve mutations to orthologs of one or more of those genes, but the phenotype did not resemble the disease.
assigned by genotype
Tg(TcrbAI4)1Dvs/0
NOD-Tg(TcrbAI4)1Dvs
- hematopoietic system phenotype
- abnormal CD4-positive T cell morphology (MGI Ref ID J:93553)
- T cells expressing the transgene comprise ~22% of the total peripheral blood lymphocyte population, a significantly higher fraction of these T cells resides in the CD4 lineage rather than in the expected CD8 lineage
- homeostasis/metabolism phenotype
- abnormal circulating glucose level (MGI Ref ID J:93553)
- female NOD mice carrying this transgene exhibit a significantly reduced frequency and rate of development of IDDM
- immune system phenotype
- abnormal CD4-positive T cell morphology (MGI Ref ID J:93553)
- T cells expressing the transgene comprise ~22% of the total peripheral blood lymphocyte population, a significantly higher fraction of these T cells resides in the CD4 lineage rather than in the expected CD8 lineage
This mouse can be used to support research in many areas including:
Tcrb relatedImmunology and Inflammation Research
Autoimmunity
Research Tools
Immunology and Inflammation Research (T Cell Receptor Transgenics)
Hematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(TcrbAI4)1Dvs | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, David V Serreze | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | AI4bTg; | ||
| Mutation Made By | David Serreze, The Jackson Laboratory | ||
| Strain of Origin | NOD/ShiLt | ||
| Expressed Gene | Tcrb, T-cell receptor beta chain, mouse, laboratory | ||
| General Note |
In conjunction with Rag1tm1Mom
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| Molecular Note | The rearranged Tcrb transgene expressed in these mice is derived from the pancreatic beta cell-reactive CD8 T lymphocyte clone AI4. The transgenic construct contains a Tcrb-V2/Tcrb-D/Tcrb-J cDNA segment. [MGI Ref ID J:93553] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(TcrbAI4)1Dvs, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Additional References
Tg(TcrbAI4)1Dvs relatedChen YG; Chen J; Osborne MA; Chapman HD; Besra GS; Porcelli SA; Leiter EH; Wilson SB; Serreze DV. 2006. CD38 is required for the peripheral survival of immunotolerogenic CD4+ invariant NK T cells in nonobese diabetic mice. J Immunol 177(5):2939-47. [PubMed: 16920929] [MGI Ref ID J:112615]
DiLorenzo TP; Lieberman SM; Takaki T; Honda S; Chapman HD; Santamaria P; Serreze DV; Nathenson SG. 2002. During the early prediabetic period in NOD mice, the pathogenic CD8(+) T-cell population comprises multiple antigenic specificities. Clin Immunol 105(3):332-41. [PubMed: 12498815] [MGI Ref ID J:94192]
Graser RT; DiLorenzo TP; Wang F; Christianson GJ; Chapman HD; Roopenian DC; Nathenson SG; Serreze DV. 2000. Identification of a CD8 T cell that can independently mediate autoimmune diabetes development in the complete absence of CD4 T cell helper functions. J Immunol 164(7):3913-8. [PubMed: 10725754] [MGI Ref ID J:93553]
Leiter EH; Reifsnyder P; Driver J; Kamdar S; Choisy-Rossi C; Serreze DV; Hara M; Chervonsky A. 2007. Unexpected functional consequences of xenogeneic transgene expression in beta-cells of NOD mice. Diabetes Obes Metab 9 Suppl 2:14-22. [PubMed: 17919174] [MGI Ref ID J:127015]
Serreze DV; Choisy-Rossi CM; Grier AE; Holl TM; Chapman HD; Gahagan JR; Osborne MA; Zhang W; King BL; Brown A; Roopenian D; Marron MP. 2008. Through Regulation of TCR Expression Levels, an Idd7 Region Gene(s) Interactively Contributes to the Impaired Thymic Deletion of Autoreactive Diabetogenic CD8+ T Cells in Nonobese Diabetic Mice. J Immunol 180(5):3250-9. [PubMed: 18292549] [MGI Ref ID J:131557]
Tsui H; Chan Y; Tang L; Winer S; Cheung RK; Paltser G; Selvanantham T; Elford AR; Ellis JR; Becker DJ; Ohashi PS; Dosch HM. 2008. Targeting of pancreatic glia in type 1 diabetes. Diabetes 57(4):918-28. [PubMed: 18198358] [MGI Ref ID J:135214]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $.00 Female or Male Hemizygous or Homozygous for Tg(TcrbAI4)1Dvs - Tg/? *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $.00 Hemizygous or Homozygous for Tg(TcrbAI4)1Dvs - Tg/? x Hemizygous or Homozygous for Tg(TcrbAI4)1Dvs - Tg/?
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $.00 Female or Male Hemizygous or Homozygous for Tg(TcrbAI4)1Dvs - Tg/? *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $.00 Hemizygous or Homozygous for Tg(TcrbAI4)1Dvs - Tg/? x Hemizygous or Homozygous for Tg(TcrbAI4)1Dvs - Tg/?
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Research Strain. Availability determined by The Jackson Laboratory scientist holding the strain. |
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General Terms and Conditions
See Terms of Use
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