Strain Name:

B6;129-E2f2tm1Zubi/J

Stock Number:

004338

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Ana Zubiaga,   University of the Basque Country

Description
Mice that are homozygous for the targeted mutation are viable and normal in size. No gene product, mRNA or protein, was detected. At age 15 months mutant mice have a 27% survival rate due to diffuse autoimmune disease that resembles systemic lupus erythematosus (SLE). The phenotype includes splenomegaly by 8-12 weeks of age, glomerulonephritis, accumulated inflammatory infiltrates in the lung, liver, and skin abnormalities such as hair loss, skin wounds, erythema. Anti-dsDNA antibodies are detected in the serum. There are an increased number of mature CD8+ thymocytes and an abnormal accumulation of CD8+ Cd44high Cd69- T effector/memory cells that are autoreactive in peripheral lymphoid organs. E2F2 deficient mice appear to have normal negative selection of thymocytes but demonstrate defects in peripheral tolerance of T lymphoctes (especially Cd8+ T cells) leading to progressive autoimmune disease. This mutant mouse strain may be useful in studies of autoimmunity and may serve as model of systemic lupus erythematosus.

Development
A targeting vector containing neomycin resistance and thymidine kinase genes was used to disrupt the second exon which encodes the DNA binding and dimerization domains of the protein. The construct was electroporated into 129S1/Sv-p+Tyr+Kitl+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were backcrossed to C57BL/6 mice.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

E2f2tm1Zubi/E2f2tm1Zubi

        involves: 129S1/Sv * C57BL/6
  • mortality/aging
  • premature death
    • at 15 months, only 27% of mice are alive compared to 67% of wild-type mice   (MGI Ref ID J:76318)
  • immune system phenotype
  • abnormal immune system morphology   (MGI Ref ID J:76318)
    • abnormal T cell differentiation
      • the fraction of mature (CD4+ and, especially, CD8+) thymocytes in the thymus is increased with a decreased in immature double positive thymocytes   (MGI Ref ID J:76318)
      • increased T cell proliferation
        • in response to IL-2 treatment, effector/memory cell (CD44hiCD69-) T cell proliferation is increased relative to in wild-type mice by 2 times at weeks 8 to 12, and 13 times at 15 months   (MGI Ref ID J:76318)
        • T cell proliferation stimulated by CD3 antibody is increased compared to in stimulated wild-type mice and is more prominent at suboptimal CD3 antibody concentrations   (MGI Ref ID J:76318)
    • abnormal T cell subpopulation ratio
      • there is an increase in the number of CD8+ T cells relative to CD4+ T cells (CD4+/CD8+ ratio: 0.79+/-0.1 compared to 1.43+/-0.3 in wild-type mice)   (MGI Ref ID J:76318)
      • however, the T to B cells ratio is normal   (MGI Ref ID J:76318)
    • abnormal spleen white pulp morphology
      • at 15 months, mice display white pulp hyperplasia and increased sinusoidal cellularity   (MGI Ref ID J:76318)
    • enlarged spleen
      • at 15 months, mice exhibit splenomegaly with spleen size 2 to 5 times greater than that of wild-type mice   (MGI Ref ID J:76318)
      • increased spleen weight
        • at week 8 to 12, spleen weight is increased 2-fold (1445+/-250 mg) relative to in wild-type mice (668+/-108 mg) and continues to increase at 15 months (3340+/-540 mg compared to 722+/-125 mg in wild-type mice)   (MGI Ref ID J:76318)
    • increased memory T cell number
      • at 3 to 4 weeks and at 15 months, the number of CD44hiCD69- memory T cells is increased   (MGI Ref ID J:76318)
  • abnormal immune system physiology   (MGI Ref ID J:76318)
    • increased anti-double stranded DNA antibody level
      • the amount of double stranded DNA antibodies is increased relative to in wild-type mice and corresponds to the severity of organ damage   (MGI Ref ID J:76318)
    • increased inflammatory response
      • at 15 months, mice exhibit features of autoimmune disease   (MGI Ref ID J:76318)
      • in elderly mice, increased mononuclear infiltrate is observed in the lung, kidney and liver   (MGI Ref ID J:76318)
      • glomerulonephritis
        • elderly mice exhibit membranoproliferative glomerulonephritis that is focal and of moderate intensity   (MGI Ref ID J:76318)
        • affected glomeruli are enlarged with a thickened basement membrane and contain perivascular aggregates of inflammatory infiltrate and immune complex deposition   (MGI Ref ID J:76318)
      • liver inflammation
        • in elderly mice, inflammatory infiltrate accumulates in the liver and perivascular infiltrates are observed   (MGI Ref ID J:76318)
      • lung inflammation
        • in elderly mice, inflammatory infiltrate accumulates in the lung   (MGI Ref ID J:76318)
    • increased susceptibility to autoimmune disorder
      • at 15 months, mice exhibit features of autoimmune disease including inflammatory infiltrate, adnormal accumulation of effector/memory T cells, and double stranded DNA antibodies   (MGI Ref ID J:76318)
  • liver/biliary system phenotype
  • liver inflammation
    • in elderly mice, inflammatory infiltrate accumulates in the liver and perivascular infiltrates are observed   (MGI Ref ID J:76318)
  • renal/urinary system phenotype
  • glomerulonephritis
    • elderly mice exhibit membranoproliferative glomerulonephritis that is focal and of moderate intensity   (MGI Ref ID J:76318)
    • affected glomeruli are enlarged with a thickened basement membrane and contain perivascular aggregates of inflammatory infiltrate and immune complex deposition   (MGI Ref ID J:76318)
  • increased renal glomerulus basement membrane thickness
    • affected glomeruli display a thickened basement membrane   (MGI Ref ID J:76318)
  • renal glomerulus hypertrophy
    • affected glomeruli are enlarged   (MGI Ref ID J:76318)
  • respiratory system phenotype
  • lung inflammation
    • in elderly mice, inflammatory infiltrate accumulates in the lung   (MGI Ref ID J:76318)
  • hematopoietic system phenotype
  • abnormal T cell differentiation
    • the fraction of mature (CD4+ and, especially, CD8+) thymocytes in the thymus is increased with a decreased in immature double positive thymocytes   (MGI Ref ID J:76318)
    • increased T cell proliferation
      • in response to IL-2 treatment, effector/memory cell (CD44hiCD69-) T cell proliferation is increased relative to in wild-type mice by 2 times at weeks 8 to 12, and 13 times at 15 months   (MGI Ref ID J:76318)
      • T cell proliferation stimulated by CD3 antibody is increased compared to in stimulated wild-type mice and is more prominent at suboptimal CD3 antibody concentrations   (MGI Ref ID J:76318)
  • abnormal T cell subpopulation ratio
    • there is an increase in the number of CD8+ T cells relative to CD4+ T cells (CD4+/CD8+ ratio: 0.79+/-0.1 compared to 1.43+/-0.3 in wild-type mice)   (MGI Ref ID J:76318)
    • however, the T to B cells ratio is normal   (MGI Ref ID J:76318)
  • abnormal spleen white pulp morphology
    • at 15 months, mice display white pulp hyperplasia and increased sinusoidal cellularity   (MGI Ref ID J:76318)
  • enlarged spleen
    • at 15 months, mice exhibit splenomegaly with spleen size 2 to 5 times greater than that of wild-type mice   (MGI Ref ID J:76318)
    • increased spleen weight
      • at week 8 to 12, spleen weight is increased 2-fold (1445+/-250 mg) relative to in wild-type mice (668+/-108 mg) and continues to increase at 15 months (3340+/-540 mg compared to 722+/-125 mg in wild-type mice)   (MGI Ref ID J:76318)
  • increased memory T cell number
    • at 3 to 4 weeks and at 15 months, the number of CD44hiCD69- memory T cells is increased   (MGI Ref ID J:76318)
  • cellular phenotype
  • abnormal cell physiology
    • adenovirus-Myc fails to induce S phase as it does in wild-type mouse embryonic fibroblasts   (MGI Ref ID J:75726)
    • abnormal cell death
      • cell death induced by Myc expression in primary fibroblast cells is somewhat less efficient than in wild-type primary fibroblast   (MGI Ref ID J:75726)
  • integument phenotype
  • abnormal skin morphology
    • elderly mice exhibit skin wounds   (MGI Ref ID J:76318)
    • reddish skin
      • elderly mice exhibit erythema affecting the head and neck   (MGI Ref ID J:76318)
  • progressive hair loss
    • elderly mice exhibit considerable hair loss   (MGI Ref ID J:76318)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

E2f2tm1Zubi related

Apoptosis Research
Endogenous Regulators
      Autoimmune Lymphroliferatve syndrome

Cancer Research
Genes Regulating Growth and Proliferation
Increased Tumor Incidence
      Lymphomas
      Lymphomas: B cell lymphomas
      Lymphomas: thymic

Cell Biology Research
Cell Cycle Regulation
Genes Regulating Growth and Proliferation

Dermatology Research
Skin and Hair Texture Defects

Developmental Biology Research
Lymphoid Tissue Defects
      hematopoietic defects

Hematological Research
Hematopoietic Defects
Immunological Defects
      B and T cell deficiency

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
      lupus erythematosus
Immunodeficiency
      T cell deficiency
Inflammation
Lymphoid Tissue Defects
      B and T cell deficiency
      hematopoietic development
      myeloid hyperplasia
T Cell Receptor Signaling Defects
      B and T cell deficiency

Internal/Organ Research
Lymphoid Tissue Defects
      T cell deficiency
Spleen Defects
Thymus Defects
      B and T cell deficient

Research Tools
Apoptosis Research
Cancer Research
      B and T cell deficiency, xenograft/transplant host
      genes regulating lymphoma development
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Production of Targeted Mutations (Knockouts)
      Mutagenesis and Transgenesis: transcriptional activation
Hematological Research
Immunology, Inflammation and Autoimmunity Research
      B cell lymphomas
      T cell deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol E2f2tm1Zubi
Allele Name targeted mutation 1, Ana Zubiaga
Allele Type Targeted (Null/Knockout)
Common Name(s) E2F2-; E2f2tm1Gle;
Mutation Made By Ana Zubiaga,   University of the Basque Country
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line NameCJ7
ES Cell Line Strain129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name E2f2, E2F transcription factor 2
Chromosome 4
Gene Common Name(s) E2F-2;
Molecular Note A PGK-neo cassette was inserted into exon 3. Southern and Northern analysis was utilized to confirm the targeting event and lack of transcript in homozygous mutant animals, respectively. [MGI Ref ID J:75726] [MGI Ref ID J:76318]

Genotyping

Genotyping Information

Genotyping Protocols

E2f2tm1Zubi, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Murga M; Fernandez-Capetillo O; Field SJ; Moreno B; Borlado LR; Fujiwara Y; Balomenos D; Vicario A; Carrera AC; Orkin SH; Greenberg ME; Zubiaga AM. 2001. Mutation of E2F2 in mice causes enhanced T lymphocyte proliferation, leading to the development of autoimmunity. Immunity 15(6):959-70. [PubMed: 11754817]  [MGI Ref ID J:76318]

Additional References

Wu L; Timmers C; Maiti B; Saavedra HI; Sang L; Chong GT; Nuckolls F; Giangrande P; Wright FA; Field SJ; Greenberg ME; Orkin S; Nevins JR; Robinson ML; Leone G. 2001. The E2F1-3 transcription factors are essential for cellular proliferation. Nature 414(6862):457-62. [PubMed: 11719808]  [MGI Ref ID J:73374]

Zhu JW; Field SJ; Gore L; Thompson M; Yang H; Fujiwara Y; Cardiff RD; Greenberg M; Orkin SH; DeGregori J. 2001. E2F1 and E2F2 Determine Thresholds for Antigen-Induced T-Cell Proliferation and Suppress Tumorigenesis. Mol Cell Biol 21(24):8547-64. [PubMed: 11713289]  [MGI Ref ID J:72952]

E2f2tm1Zubi related

Andrechek ER; Mori S; Rempel RE; Chang JT; Nevins JR. 2008. Patterns of cell signaling pathway activation that characterize mammary development. Development 135(14):2403-13. [PubMed: 18550711]  [MGI Ref ID J:137644]

Bilousova G; Marusyk A; Porter CC; Cardiff RD; DeGregori J. 2005. Impaired DNA replication within progenitor cell pools promotes leukemogenesis. PLoS Biol 3(12):e401. [PubMed: 16277552]  [MGI Ref ID J:103814]

Chen D; Opavsky R; Pacal M; Tanimoto N; Wenzel P; Seeliger MW; Leone G; Bremner R. 2007. Rb-Mediated Neuronal Differentiation through Cell-Cycle-Independent Regulation of E2f3a. PLoS Biol 5(7):e179. [PubMed: 17608565]  [MGI Ref ID J:124204]

Chen HZ; Ouseph MM; Li J; Pecot T; Chokshi V; Kent L; Bae S; Byrne M; Duran C; Comstock G; Trikha P; Mair M; Senapati S; Martin CK; Gandhi S; Wilson N; Liu B; Huang YW; Thompson JC; Raman S; Singh S; Leone M; Machiraju R; Huang K; Mo X; Fernandez S; Kalaszczynska I; Wolgemuth DJ; Sicinski P; Huang T; Jin V; Leone G. 2012. Canonical and atypical E2Fs regulate the mammalian endocycle. Nat Cell Biol 14(11):1192-202. [PubMed: 23064266]  [MGI Ref ID J:190713]

Chen Q; Khoury M; Chen J. 2009. Expression of human cytokines dramatically improves reconstitution of specific human-blood lineage cells in humanized mice. Proc Natl Acad Sci U S A :. [PubMed: 19966223]  [MGI Ref ID J:155817]

Chong JL; Tsai SY; Sharma N; Opavsky R; Price R; Wu L; Fernandez SA; Leone G. 2009. E2f3a and E2f3b contribute to the control of cell proliferation and mouse development. Mol Cell Biol 29(2):414-24. [PubMed: 19015245]  [MGI Ref ID J:144747]

Chong JL; Wenzel PL; Saenz-Robles MT; Nair V; Ferrey A; Hagan JP; Gomez YM; Sharma N; Chen HZ; Ouseph M; Wang SH; Trikha P; Culp B; Mezache L; Winton DJ; Sansom OJ; Chen D; Bremner R; Cantalupo PG; Robinson ML; Pipas JM; Leone G. 2009. E2f1-3 switch from activators in progenitor cells to repressors in differentiating cells. Nature 462(7275):930-4. [PubMed: 20016602]  [MGI Ref ID J:155799]

DeRyckere D; DeGregori J. 2005. E2F1 and E2F2 are differentially required for homeostasis-driven and antigen-induced T cell proliferation in vivo. J Immunol 175(2):647-55. [PubMed: 16002659]  [MGI Ref ID J:101673]

Dirlam A; Spike BT; Macleod KF. 2007. Deregulated E2f-2 underlies cell cycle and maturation defects in retinoblastoma null erythroblasts. Mol Cell Biol 27(24):8713-28. [PubMed: 17923680]  [MGI Ref ID J:129010]

Fujiwara K; Yuwanita I; Hollern DP; Andrechek ER. 2011. Prediction and Genetic Demonstration of a Role for Activator E2Fs in Myc-Induced Tumors. Cancer Res 71(5):1924-32. [PubMed: 21245101]  [MGI Ref ID J:169307]

Iglesias A; Murga M; Laresgoiti U; Skoudy A; Bernales I; Fullaondo A; Moreno B; Lloreta J; Field SJ; Real FX; Zubiaga AM. 2004. Diabetes and exocrine pancreatic insufficiency in E2F1/E2F2 double-mutant mice. J Clin Invest 113(10):1398-407. [PubMed: 15146237]  [MGI Ref ID J:91105]

Iglesias-Ara A; Zenarruzabeitia O; Fernandez-Rueda J; Sanchez-Tillo E; Field SJ; Celada A; Zubiaga AM. 2010. Accelerated DNA replication in E2F1- and E2F2-deficient macrophages leads to induction of the DNA damage response and p21(CIP1)-dependent senescence. Oncogene 29(41):5579-90. [PubMed: 20676136]  [MGI Ref ID J:165294]

Leone G; Sears R; Huang E; Rempel R; Nuckolls F; Park CH; Giangrande P; Wu L; Saavedra HI; Field SJ; Thompson MA; Yang H; Fujiwara Y; Greenberg ME; Orkin S; Smith C; Nevins JR. 2001. Myc requires distinct E2F activities to induce S phase and apoptosis. Mol Cell 8(1):105-13. [PubMed: 11511364]  [MGI Ref ID J:75726]

Li FX; Zhu JW; Hogan CJ; DeGregori J. 2003. Defective gene expression, S phase progression, and maturation during hematopoiesis in E2F1/E2F2 mutant mice. Mol Cell Biol 23(10):3607-22. [PubMed: 12724419]  [MGI Ref ID J:83284]

Li FX; Zhu JW; Tessem JS; Beilke J; Varella-Garcia M; Jensen J; Hogan CJ; DeGregori J. 2003. The development of diabetes in E2f1/E2f2 mutant mice reveals important roles for bone marrow-derived cells in preventing islet cell loss. Proc Natl Acad Sci U S A 100(22):12935-40. [PubMed: 14566047]  [MGI Ref ID J:86404]

Opavsky R; Tsai SY; Guimond M; Arora A; Opavska J; Becknell B; Kaufmann M; Walton NA; Stephens JA; Fernandez SA; Muthusamy N; Felsher DW; Porcu P; Caligiuri MA; Leone G. 2007. Specific tumor suppressor function for E2F2 in Myc-induced T cell lymphomagenesis. Proc Natl Acad Sci U S A 104(39):15400-5. [PubMed: 17881568]  [MGI Ref ID J:125304]

Palmero I; Murga M; Zubiaga A; Serrano M. 2002. Activation of ARF by oncogenic stress in mouse fibroblasts is independent of E2F1 and E2F2. Oncogene 21(19):2939-47. [PubMed: 12082524]  [MGI Ref ID J:126187]

Redmond WL; Wei CH; Kreuwel HT; Sherman LA. 2008. The apoptotic pathway contributing to the deletion of naive CD8 T cells during the induction of peripheral tolerance to a cross-presented self-antigen. J Immunol 180(8):5275-82. [PubMed: 18390708]  [MGI Ref ID J:134242]

Rempel RE; Mori S; Gasparetto M; Glozak MA; Andrechek ER; Adler SB; Laakso NM; Lagoo AS; Storms R; Smith C; Nevins JR. 2009. A role for E2F activities in determining the fate of Myc-induced lymphomagenesis. PLoS Genet 5(9):e1000640. [PubMed: 19749980]  [MGI Ref ID J:154120]

Saenz Robles MT; Case A; Chong JL; Leone G; Pipas JM. 2011. The retinoblastoma tumor suppressor regulates a xenobiotic detoxification pathway. PLoS One 6(10):e26019. [PubMed: 22022495]  [MGI Ref ID J:178100]

Saenz-Robles MT; Markovics JA; Chong JL; Opavsky R; Whitehead RH; Leone G; Pipas JM. 2007. Intestinal hyperplasia induced by simian virus 40 large tumor antigen requires E2F2. J Virol 81(23):13191-9. [PubMed: 17855529]  [MGI Ref ID J:129937]

Sharma N; Timmers C; Trikha P; Saavedra HI; Obery A; Leone G. 2006. Control of the p53-p21CIP1 Axis by E2f1, E2f2, and E2f3 is essential for G1/S progression and cellular transformation. J Biol Chem 281(47):36124-31. [PubMed: 17008321]  [MGI Ref ID J:117634]

Tessem JS; Jensen JN; Pelli H; Dai XM; Zong XH; Stanley ER; Jensen J; DeGregori J. 2008. Critical roles for macrophages in islet angiogenesis and maintenance during pancreatic degeneration. Diabetes 57(6):1605-17. [PubMed: 18375440]  [MGI Ref ID J:136898]

Trikha P; Sharma N; Opavsky R; Reyes A; Pena C; Ostrowski MC; Roussel MF; Leone G. 2011. E2f1-3 are critical for myeloid development. J Biol Chem 286(6):4783-95. [PubMed: 21115501]  [MGI Ref ID J:169491]

Tsai SY; Opavsky R; Sharma N; Wu L; Naidu S; Nolan E; Feria-Arias E; Timmers C; Opavska J; de Bruin A; Chong JL; Trikha P; Fernandez SA; Stromberg P; Rosol TJ; Leone G. 2008. Mouse development with a single E2F activator. Nature 454(7208):1137-41. [PubMed: 18594513]  [MGI Ref ID J:138289]

Wenzel PL; Chong JL; Saenz-Robles MT; Ferrey A; Hagan JP; Gomez YM; Rajmohan R; Sharma N; Chen HZ; Pipas JM; Robinson ML; Leone G. 2011. Cell proliferation in the absence of E2F1-3. Dev Biol 351(1):35-45. [PubMed: 21185283]  [MGI Ref ID J:170580]

Wu L; Timmers C; Maiti B; Saavedra HI; Sang L; Chong GT; Nuckolls F; Giangrande P; Wright FA; Field SJ; Greenberg ME; Orkin S; Nevins JR; Robinson ML; Leone G. 2001. The E2F1-3 transcription factors are essential for cellular proliferation. Nature 414(6862):457-62. [PubMed: 11719808]  [MGI Ref ID J:73374]

Yan Z; Choi S; Liu X; Zhang M; Schageman JJ; Lee SY; Hart R; Lin L; Thurmond FA; Williams RS. 2003. Highly coordinated gene regulation in mouse skeletal muscle regeneration. J Biol Chem 278(10):8826-36. [PubMed: 12477723]  [MGI Ref ID J:82190]

Zencak D; Schouwey K; Chen D; Ekstrom P; Tanger E; Bremner R; van Lohuizen M; Arsenijevic Y. 2013. Retinal degeneration depends on Bmi1 function and reactivation of cell cycle proteins. Proc Natl Acad Sci U S A 110(7):E593-601. [PubMed: 23359713]  [MGI Ref ID J:194322]

Zhang J; Bahi N; Zubiaga AM; Comella JX; Llovera M; Sanchis D. 2007. Developmental silencing and independency from E2F of apoptotic gene expression in postmitotic tissues. FEBS Lett 581(30):5781-6. [PubMed: 18037375]  [MGI Ref ID J:129205]

Zhou J; Wu M; Xu S; Cheng M; Ding C; Liu Y; Yan H; Biyashev D; Kishore R; Qin G. 2013. Contrasting roles of E2F2 and E2F3 in cardiac neovascularization. PLoS One 8(6):e65755. [PubMed: 23799044]  [MGI Ref ID J:204212]

Zhou J; Zhu Y; Cheng M; Dinesh D; Thorne T; Poh KK; Liu D; Botros C; Tang YL; Reisdorph N; Kishore R; Losordo DW; Qin G. 2009. Regulation of vascular contractility and blood pressure by the E2F2 transcription factor. Circulation 120(13):1213-21. [PubMed: 19752322]  [MGI Ref ID J:167500]

Zhu JW; Field SJ; Gore L; Thompson M; Yang H; Fujiwara Y; Cardiff RD; Greenberg M; Orkin SH; DeGregori J. 2001. E2F1 and E2F2 Determine Thresholds for Antigen-Induced T-Cell Proliferation and Suppress Tumorigenesis. Mol Cell Biol 21(24):8547-64. [PubMed: 11713289]  [MGI Ref ID J:72952]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6;129 background and is maintained on the same as a homozygote.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

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Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use


General Terms and Conditions


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phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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