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Strain Name:

NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs/DvsJ

Stock Number:

004347

Availability:

Repository-Cryopreserved

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Former Name      NOD.Cg-Tg(TcraAI4)1Dvs Rag1tm1Mom/DvsJ    (Changed: 15-DEC-04 )
Genes & Alleles   Rag1;   Rag1tm1Mom;   Tcra;   Tg(TcraAI4)1Dvs;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Background Strain NOD/ShiLt
Donating Investigator David Serreze,   The Jackson Laboratory
H2 Haplotypeg7
GenerationF7+1p (25-JUL-04)

Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/Tyrc

Strain Description
The rearranged Tcra transgene expressed in these mice is derived from the pancreatic beta cell-reactive CD8 T lymphocyte clone AI4. The presence of the nonfunctional Rag1tm1Mom allele renders mice incapable of rearranging endogenous T cell receptor genes. When crossed with strain NOD.Cg-Tg(TcrbAI4)1DvsRag1tm1Mom/DvsJ (004348), the resulting animals that carry both the alpha and beta AI4 TCR transgenes exhibit an accelerated development of insulin-dependent diabetes mellitus (IDDM). Additionally, transgenic animals bearing both TCR transgenes offer a source of CTL precursors useful in examining the diversity of beta cell peptides recognized by the autoreactive CD8+ T lymphocytes contributing to the earliest phase of IDDM development. Homozygous mice are viable and fertile.

Strain Development
A transgenic construct containing a Tcra-V8/Tcra-J cDNA segment was injected into NOD/Lt zygotes. An outcross-intercross strategy was employed to introduce an inactivated Rag1 allele from an N11 congenic stock, NOD.129S7(B6)-Rag1tm1Mom/J mice (strain 003729).

Gene & Allele Details

Allele Symbol Rag1tm1Mom
Allele Name targeted mutation 1, Peter Mombaerts
Common Name(s) RAG-1-; RAG1null; Rag-; Rag-1KO; Rag1-;
Mutation Made By Peter Mombaerts,   Max Planck Institute of Biophysics
Strain of Origin129S7/SvEvBrd-Hprt1<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt1<+>
Gene Symbol and Name Rag1, recombination activating gene 1
Chromosome 2
Gene Common Name(s) MGC43321; RNF74; Rag-1;
Molecular Note A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice. [MGI Ref ID J:1934] [MGI Ref ID J:96036]
 
Allele Symbol Tg(TcraAI4)1Dvs
Allele Name transgene insertion 1, David V Serreze
Common Name(s) AI4aTg;
Mutation Made By David Serreze,   The Jackson Laboratory
Strain of OriginNOD/ShiLt
Expressed Gene Tcra, T-cell receptor alpha chain, mouse, laboratory
General Note In conjunction with Rag1tm1Mom
    transgenic mice carrying both the alpha and beta AI4 TCR transgenes exhibit an accelerated development of insulin-dependent diabetes mellitus (IDDM).
    homozygous mice are viable and fertile
Molecular Note The rearranged Tcra transgene expressed in these mice is derived from the pancreatic beta cell-reactive CD8 T lymphocyte clone AI4. The transgene contains a Tcra-V8/Tcra-J cDNA segment. [MGI Ref ID J:93553]

Control Information

  Control
   Rag1/Rag1, +/+ from the colony
   003729 NOD.129S7(B6)-Rag1tm1Mom/J
 
  Considerations for Choosing Controls

Genotyping Protocols

Rag1tm1Mom

Colony Maintenance

Breeding & HusbandryThese mice are maintained homozygote for Rag1 and are severely immunodeficient, so specific pathogen-free (SPF) conditions are recommended. This strain is maintaned through mating Rag1-/Rag1-, Tg/? x Rag1-/Rag1-, Tg/?. Genotypes available are Rag1-/Rag1-, Tg? or Rag1-/Rag1-, +/+. breeder pairs available: Rag1-/Rag1-, Tg/? x Rag1-/Rag1-, Tg/?. Affected mutant is Rag1-/Rag1-, Tg/?

Related Strains

View Strains carrying   Rag1tm1Mom     (15 strains)

View Strains carrying   Tg(TcraAI4)1Dvs     (4 strains)

Strains carrying other alleles of Rag1
002194   B6;CBA-Tg(CD3E)26Cpt/J
002506   STOCK Tg(CD3E)26Cpt-Rag1tm1Mom/J
View Strains carrying other alleles of Rag1     (2 strains)

View Strains carrying other alleles of Tcra     (30 strains)

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Congenics with Mutations Affecting Immunocompetence)
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)

Research Tools
Immunology and Inflammation Research (T Cell Receptor Transgenics)

Tcra related

Hematological Research
Immunological Defects

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects

Research Tools
Cancer Research (specific T cell deficiency)

Rag1tm1Mom related

Cancer Research
Toxicology (B and T cell deficiency) (xenograft/transplant host)

Hematological Research
Immunological Defects (B and T cell deficiency)

Immunology and Inflammation Research
Immunodeficiency (B and T cell deficiency)
Inflammation (B and T cell deficiency)
T Cell Receptor Signaling Defects (B and T cell deficiency)

Internal/Organ Research
Lymphoid Tissue Defects (B and T cell deficiency)

Research Tools
Cancer Research (B and T cell deficiency) (xenograft/transplant host)
Toxicology Research (xenograft/transplant host)

References

Selected Reference(s)

DiLorenzo TP; Lieberman SM; Takaki T; Honda S; Chapman HD; Santamaria P; Serreze DV; Nathenson SG. 2002. During the early prediabetic period in NOD mice, the pathogenic CD8(+) T-cell population comprises multiple antigenic specificities. Clin Immunol 105(3):332-41. [PubMed: 12498815]  [MGI Ref ID J:94192]

Additional References

Price and Supply Information

Strain Name: NOD.Cg-Rag1tm1Mom Tg(TcraAI4)1Dvs/DvsJ
Stock Number: 004347

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Type 1 Diabetes Repository collection.

LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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