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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation F?+11 (20-DEC-06) Donating Investigator Brian Schaefer, Uniformed Services Univ. Health Sciences Description
These transgenic mice express enhanced Green Fluorescent Protein (GFP) under the direction of the human ubiqutin C promoter. Mice homozygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice express GFP in all tissues examined. Certain hematapoetic cell types display distinct expression levels of GFP, allowing identification of different cells types by FACS analysis. GFP expression is uniform within a cell type lineage and remains constant throughout development. T cells have a 2-fold higher GFP expression than CD19+B220+ B cells or peripheral blood cells. Leukocytes and red blood cells from homozygous mice fluoresce at approximately twice the level of cells from hemizygous mice. This mutant mouse strain represents a useful tool in studies related to hematopoetic cell differentiation and in vivo tracking of leukocytes.Development
A transgenic construct containing an enhanced green fluorescent protein open reading frame under the control of the human ubiqutin C promoter was microinjected into fertilized C57BL/6 oocytes. Mice were screened for genomic integration of the transgene by PCR and for GFP expression in peripheral blood leukocytes. The resulting female mouse exhibiting the desired phenotype was bred to a male C57BL/6J mouse. F1 progeny from this cross that expressed GFP were intercrossed to produce homozygotes.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
007076 CByJ.B6-Tg(UBC-GFP)30Scha/J
007179 129S.Cg-Tg(UBC-cre/ESR1)1Ejb/J 006259 B6.Cg-Pepcb Ptprca Tg(UBC-scFv)2Nemz/J 008085 B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J 007001 B6;129S-Tg(UBC-cre/ESR1)1Ejb/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
JAX® NOTES, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Hematological Research
Immunology and Inflammation Research
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(UBC-GFP)30Scha | ||
|---|---|---|---|
| Allele Name | transgene insertion 30, Brian Schaefer | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | UBC-GFP; UBI-EGFP; UBI-GFP; uGFP; | ||
| Mutation Made By | Brian Schaefer, Uniformed Services Univ. Health Sciences | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | Certain hematopoietic cell types display distinct expression levels of GFP. Expression is uniform within a cell type lineage and remains constant throughout development. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | UBC, ubiquitin C, human | ||
| Gene Symbol and Name | Tg(UBC-GFP)30Scha, transgene insertion 30, Brian Schaefer | ||
| Chromosome | UN | ||
| Gene Common Name(s) | UBC-GFP; UBI-EGFP; UBI-GFP; uGFP; | ||
| General Note | Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These mice express GFP in all tissues examined. Expression levels vary between certain hematapoetic cell types. GFP expression is uniform within a cell type lineage and remains constant throughout development. T cells have a 2-fold higher GFP expression than CD19+B220+ B cells or peripheral blood cells. Leukocytes and red blood cells from homozygous transgenic mice fluoresce at approximately twice the level of cells from hemizygous mice. | ||
| Molecular Note | The transgene contains an enhanced green fluorescent protein open reading frame under the control of the human ubiquitin C promoter. [MGI Ref ID J:93490] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Fluorescent Proteins -- Generic GFP, QPCR
NntC57BL/6J, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Schaefer BC; Schaefer ML; Kappler JW; Marrack P; Kedl RM. 2001. Observation of antigen-dependent CD8+ T-cell/ dendritic cell interactions in vivo. Cell Immunol 214(2):110-22. [PubMed: 12088410] [MGI Ref ID J:93490]
Additional References
Tg(UBC-GFP)30Scha relatedAkk AM; Simmons PM; Chan HW; Agapov E; Holtzman MJ; Grayson MH; Pham CT. 2008. Dipeptidyl peptidase I-dependent neutrophil recruitment modulates the inflammatory response to sendai virus infection. J Immunol 180(5):3535-42. [PubMed: 18292580] [MGI Ref ID J:131520]
Allen CD; Okada T; Tang HL; Cyster JG. 2007. Imaging of germinal center selection events during affinity maturation. Science 315(5811):528-31. [PubMed: 17185562] [MGI Ref ID J:118931]
Bajenoff M; Egen JG; Koo LY; Laugier JP; Brau F; Glaichenhaus N; Germain RN. 2006. Stromal cell networks regulate lymphocyte entry, migration, and territoriality in lymph nodes. Immunity 25(6):989-1001. [PubMed: 17112751] [MGI Ref ID J:116602]
Bajenoff M; Glaichenhaus N; Germain RN. 2008. Fibroblastic reticular cells guide T lymphocyte entry into and migration within the splenic T cell zone. J Immunol 181(6):3947-54. [PubMed: 18768849] [MGI Ref ID J:139103]
Byrne SN; Limon-Flores AY; Ullrich SE. 2008. Mast Cell Migration from the Skin to the Draining Lymph Nodes upon Ultraviolet Irradiation Represents a Key Step in the Induction of Immune Suppression. J Immunol 180(7):4648-55. [PubMed: 18354188] [MGI Ref ID J:133390]
Fancke B; Suter M; Hochrein H; O'Keeffe M. 2008. M-CSF: a novel plasmacytoid and conventional dendritic cell poietin. Blood 111(1):150-9. [PubMed: 17916748] [MGI Ref ID J:130107]
Fee D; Crumbaugh A; Jacques T; Herdrich B; Sewell D; Auerbach D; Piaskowski S; Hart MN; Sandor M; Fabry Z. 2003. Activated/effector CD4+ T cells exacerbate acute damage in the central nervous system following traumatic injury. J Neuroimmunol 136(1-2):54-66. [PubMed: 12620643] [MGI Ref ID J:119181]
Ford MS; Zhang ZX; Chen W; Zhang L. 2006. Double-negative T regulatory cells can develop outside the thymus and do not mature from CD8+ T cell precursors. J Immunol 177(5):2803-9. [PubMed: 16920915] [MGI Ref ID J:139556]
Furuhashi M; Fucho R; Gorgun CZ; Tuncman G; Cao H; Hotamisligil GS. 2008. Adipocyte/macrophage fatty acid-binding proteins contribute to metabolic deterioration through actions in both macrophages and adipocytes in mice. J Clin Invest 118(7):2640-50. [PubMed: 18551191] [MGI Ref ID J:137702]
Grigorova IL; Schwab SR; Phan TG; Pham TH; Okada T; Cyster JG. 2009. Cortical sinus probing, S1P1-dependent entry and flow-based capture of egressing T cells. Nat Immunol 10(1):58-65. [PubMed: 19060900] [MGI Ref ID J:142339]
Hisatomi T; Nakazawa T; Noda K; Almulki L; Miyahara S; Nakao S; Ito Y; She H; Kohno R; Michaud N; Ishibashi T; Hafezi-Moghadam A; Badley AD; Kroemer G; Miller JW. 2008. HIV protease inhibitors provide neuroprotection through inhibition of mitochondrial apoptosis in mice. J Clin Invest 118(6):2025-38. [PubMed: 18497877] [MGI Ref ID J:137728]
Hsu HC; Yang P; Wang J; Wu Q; Myers R; Chen J; Yi J; Guentert T; Tousson A; Stanus AL; Le TV; Lorenz RG; Xu H; Kolls JK; Carter RH; Chaplin DD; Williams RW; Mountz JD. 2008. Interleukin 17-producing T helper cells and interleukin 17 orchestrate autoreactive germinal center development in autoimmune BXD2 mice. Nat Immunol 9(2):166-75. [PubMed: 18157131] [MGI Ref ID J:131634]
Hugues S; Scholer A; Boissonnas A; Nussbaum A; Combadiere C; Amigorena S; Fetler L. 2007. Dynamic imaging of chemokine-dependent CD8(+) T cell help for CD8(+) T cell responses. Nat Immunol 8(9):921-30. [PubMed: 17660821] [MGI Ref ID J:124274]
Lannutti BJ; Epp A; Roy J; Chen J; Josephson NC. 2009. Incomplete restoration of Mpl expression in the mpl-/- mouse produces partial correction of the stem cell-repopulating defect and paradoxical thrombocytosis. Blood 113(8):1778-85. [PubMed: 18796624] [MGI Ref ID J:145455]
Naik SH; Sathe P; Park HY; Metcalf D; Proietto AI; Dakic A; Carotta S; O'Keeffe M; Bahlo M; Papenfuss A; Kwak JY; Wu L; Shortman K. 2007. Development of plasmacytoid and conventional dendritic cell subtypes from single precursor cells derived in vitro and in vivo. Nat Immunol 8(11):1217-26. [PubMed: 17922015] [MGI Ref ID J:126316]
Oliver PM; Cao X; Worthen GS; Shi P; Briones N; MacLeod M; White J; Kirby P; Kappler J; Marrack P; Yang B. 2006. Ndfip1 protein promotes the function of itch ubiquitin ligase to prevent T cell activation and T helper 2 cell-mediated inflammation. Immunity 25(6):929-40. [PubMed: 17137798] [MGI Ref ID J:116617]
Otahal P; Knowles BB; Tevethia SS; Schell TD. 2007. Anti-CD40 conditioning enhances the T(CD8) response to a highly tolerogenic epitope and subsequent immunotherapy of simian virus 40 T antigen-induced pancreatic tumors. J Immunol 179(10):6686-95. [PubMed: 17982058] [MGI Ref ID J:131746]
Otero K; Turnbull IR; Poliani PL; Vermi W; Cerutti E; Aoshi T; Tassi I; Takai T; Stanley SL; Miller M; Shaw AS; Colonna M. 2009. Macrophage colony-stimulating factor induces the proliferation and survival of macrophages via a pathway involving DAP12 and beta-catenin. Nat Immunol 10(7):734-43. [PubMed: 19503107] [MGI Ref ID J:150133]
Pagano MB; Bartoli MA; Ennis TL; Mao D; Simmons PM; Thompson RW; Pham CT. 2007. Critical role of dipeptidyl peptidase I in neutrophil recruitment during the development of experimental abdominal aortic aneurysms. Proc Natl Acad Sci U S A 104(8):2855-60. [PubMed: 17301245] [MGI Ref ID J:125910]
Phan TG; Green JA; Gray EE; Xu Y; Cyster JG. 2009. Immune complex relay by subcapsular sinus macrophages and noncognate B cells drives antibody affinity maturation. Nat Immunol 10(7):786-93. [PubMed: 19503106] [MGI Ref ID J:150134]
Reboldi A; Coisne C; Baumjohann D; Benvenuto F; Bottinelli D; Lira S; Uccelli A; Lanzavecchia A; Engelhardt B; Sallusto F. 2009. C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE. Nat Immunol 10(5):514-23. [PubMed: 19305396] [MGI Ref ID J:148286]
Rodeheffer MS; Birsoy K; Friedman JM. 2008. Identification of white adipocyte progenitor cells in vivo. Cell 135(2):240-9. [PubMed: 18835024] [MGI Ref ID J:147623]
Rork TH; Wallace KL; Kennedy DP; Marshall MA; Lankford AR; Linden J. 2008. Adenosine A2A receptor activation reduces infarct size in the isolated, perfused mouse heart by inhibiting resident cardiac mast cell degranulation. Am J Physiol Heart Circ Physiol 295(5):H1825-33. [PubMed: 18757481] [MGI Ref ID J:142451]
Ryan CM; Schell TD. 2006. Accumulation of CD8+ T cells in advanced-stage tumors and delay of disease progression following secondary immunization against an immunorecessive epitope. J Immunol 177(1):255-67. [PubMed: 16785521] [MGI Ref ID J:114942]
Schaeffer M; Han SJ; Chtanova T; van Dooren GG; Herzmark P; Chen Y; Roysam B; Striepen B; Robey EA. 2009. Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii. J Immunol 182(10):6379-93. [PubMed: 19414791] [MGI Ref ID J:148321]
Scholer A; Hugues S; Boissonnas A; Fetler L; Amigorena S. 2008. Intercellular adhesion molecule-1-dependent stable interactions between T cells and dendritic cells determine CD8+ T cell memory. Immunity 28(2):258-70. [PubMed: 18275834] [MGI Ref ID J:132214]
Seandel M; James D; Shmelkov SV; Falciatori I; Kim J; Chavala S; Scherr DS; Zhang F; Torres R; Gale NW; Yancopoulos GD; Murphy A; Valenzuela DM; Hobbs RM; Pandolfi PP; Rafii S. 2007. Generation of functional multipotent adult stem cells from GPR125+ germline progenitors. Nature 449(7160):346-50. [PubMed: 17882221] [MGI Ref ID J:126330]
Shaked Y; Ciarrocchi A; Franco M; Lee CR; Man S; Cheung AM; Hicklin DJ; Chaplin D; Foster FS; Benezra R; Kerbel RS. 2006. Therapy-induced acute recruitment of circulating endothelial progenitor cells to tumors. Science 313(5794):1785-7. [PubMed: 16990548] [MGI Ref ID J:129431]
Shiow LR; Roadcap DW; Paris K; Watson SR; Grigorova IL; Lebet T; An J; Xu Y; Jenne CN; Foger N; Sorensen RU; Goodnow CC; Bear JE; Puck JM; Cyster JG. 2008. The actin regulator coronin 1A is mutant in a thymic egress-deficient mouse strain and in a patient with severe combined immunodeficiency. Nat Immunol 9(11):1307-15. [PubMed: 18836449] [MGI Ref ID J:141431]
Van Eck M; Ye D; Hildebrand RB; Kar Kruijt J; de Haan W; Hoekstra M; Rensen PC; Ehnholm C; Jauhiainen M; Van Berkel TJ. 2007. Important role for bone marrow-derived cholesteryl ester transfer protein in lipoprotein cholesterol redistribution and atherosclerotic lesion development in LDL receptor knockout mice. Circ Res 100(5):678-85. [PubMed: 17293475] [MGI Ref ID J:133704]
Wallace KL; Marshall MA; Ramos SI; Lannigan JA; Field JJ; Strieter RM; Linden J. 2009. NKT cells mediate pulmonary inflammation and dysfunction in murine sickle cell disease through production of IFN-gamma and CXCR3 chemokines. Blood 114(3):667-76. [PubMed: 19433855] [MGI Ref ID J:150754]
Weiser-Evans MC; Wang XQ; Amin J; Van Putten V; Choudhary R; Winn RA; Scheinman R; Simpson P; Geraci MW; Nemenoff RA. 2009. Depletion of cytosolic phospholipase A2 in bone marrow-derived macrophages protects against lung cancer progression and metastasis. Cancer Res 69(5):1733-8. [PubMed: 19208832] [MGI Ref ID J:146441]
Woolf E; Grigorova I; Sagiv A; Grabovsky V; Feigelson SW; Shulman Z; Hartmann T; Sixt M; Cyster JG; Alon R. 2007. Lymph node chemokines promote sustained T lymphocyte motility without triggering stable integrin adhesiveness in the absence of shear forces. Nat Immunol 8(10):1076-85. [PubMed: 17721537] [MGI Ref ID J:125276]
Yurchenko E; Friedman H; Hay V; Peterson A; Piccirillo CA. 2007. Ubiquitous expression of mRFP-1 in vivo by site-directed transgenesis. Transgenic Res 16(1):29-40. [PubMed: 17077985] [MGI Ref ID J:117726]
Zozulya AL; Reinke E; Baiu DC; Karman J; Sandor M; Fabry Z. 2007. Dendritic cell transmigration through brain microvessel endothelium is regulated by MIP-1alpha chemokine and matrix metalloproteinases. J Immunol 178(1):520-9. [PubMed: 17182592] [MGI Ref ID J:141921]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX1
Colony Maintenance
Breeding & Husbandry This strain originated on a C57BL/6 background and is maintained as a homozygote. Coat color expected from breeding:Black Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided 3-5 weeks $101.30 Female or Male Homozygous for Tg(UBC-GFP)30Scha 6 weeks $105.55 Female or Male Homozygous for Tg(UBC-GFP)30Scha 7 weeks $109.80 Female or Male Homozygous for Tg(UBC-GFP)30Scha 8 weeks $114.05 Female or Male Homozygous for Tg(UBC-GFP)30Scha
Pairs /Price (US dollars $) Pair Genotype $211.10 Homozygous for Tg(UBC-GFP)30Scha x Homozygous for Tg(UBC-GFP)30Scha
Additional Supply Details
| Pricing for International shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided 3-5 weeks $131.70 Female or Male Homozygous for Tg(UBC-GFP)30Scha 6 weeks $137.30 Female or Male Homozygous for Tg(UBC-GFP)30Scha 7 weeks $142.90 Female or Male Homozygous for Tg(UBC-GFP)30Scha 8 weeks $148.50 Female or Male Homozygous for Tg(UBC-GFP)30Scha
Pairs /Price (US dollars $) Pair Genotype $274.50 Homozygous for Tg(UBC-GFP)30Scha x Homozygous for Tg(UBC-GFP)30Scha
Additional Supply Details
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Supply Details
| Standard Supply | Level 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one to three months. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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