Strain Name: |
B6;129-Scarb1tm1Kri Apoetm1Unc/J |
|---|---|
Stock Number: |
004362 |
Availability: | Repository-Cryopreserved |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
B6;129-Srb1tm1Kri Apoetm1Unc (Changed: 15-DEC-04
) |
| Genes & Alleles | Apoe; Apoetm1Unc; Scarb1; Scarb1tm1Kri; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Strain Description
At birth, mice homozygous for both targeted alleles are viable, normal in size and do not display any gross physical or behavioral abnormalities. All double mutants die between 5 to 8 weeks following birth, exhibiting hypercholesterolemia and significantly lower body weights compared to control mice. Mice display an altered appearance several days before death, displaying ruffled fur, an abnormal gate and sometimes labored breathing. Hearts from double mutants are enlarged, exhibit evidence of myocardial infarction and are functionally impaired. Histological examination reveals extensive coronary artery disease characterized by atherosclerotic plaques with evidence of cholesterol clefts and fibrin deposition. As with other Scarb1 homozygous null mutants, females are infertile while the fertility of homozygous males remains intact. This double mutant mouse strain offers a model that may be a useful tool in studies related to coronary heart disease.Strain Development
Please see strain entries 002052 and 003379 for details regarding the construction of each mutant allele.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Apoetm1Unc | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, University of North Carolina | ||
| Common Name(s) | APOE KO; AopE(-); ApoE-KO; Apoetm1Un; apoE-; apoE0; epsilon-; | ||
| Mutation Made By | Nobuyo Maeda, Univ of North Carolina at Chapel Hill | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14TG2a | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Apoe, apolipoprotein E | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | AD2; AI255918; LPG; MGC1571; apoprotein; expressed sequence AI255918; | ||
| General Note |
In Apoetm1Unc mutant mice also carrying Csf1op, atherotic lesions of the proximal aorta were abolished or significantly reduced in size relative to Apoe mutants homozygous for the normal allele at Csf1 (J:40136). The results may have been due to reduced macrophages or M-CSF, but were not due to reductions in circulating lipoprotein (J:40136). | ||
| Molecular Note | Insertion of a neomycin resistance cassette deleted part of exon 3 and part of intron 3 of the Apoe gene. Plasma from homozygous mutant mice gave no detectable immunoprecipitate by the Ouchterlony double immunodiffusion test using a rabbit antibody to rat APOE. [MGI Ref ID J:1050] | ||
| Allele Symbol | Scarb1tm1Kri | ||
| Allele Name | targeted mutation 1, Monty Krieger | ||
| Common Name(s) | SR-BI; SR-BI-; srb1-; srbI-; | ||
| Mutation Made By | Monty Krieger, Massachusetts Institute of Technology | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | Scarb1, scavenger receptor class B, member 1 | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | AI120173; CD36L1; CLA-1; CLA1; D5Ertd460e; DNA segment, Chr 5, ERATO Doi 460, expressed; MGC138242; SR-B1; SR-BI; SRB1; SRBI; Srb1; expressed sequence AI120173; scavenger receptor class B1; | ||
| Molecular Note | Replacement of the entire coding region of the first exon and an additional 554 bases of intron 1 with a neomycin cassette. [MGI Ref ID J:44187] | ||
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Apoetm1Unc
Scarb1tm1Kri
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, this strain is maintained by mating Apoe/Apoe Scarb1/+ x Apoe/Apoe +/+ or reciprocal. Note: mice homozygous for both null alleles die between 5 to 8 weeks. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Apoetm1Unc allele
View Strains carrying Apoetm1Unc (9 strains)
003379 B6;129S2-Scarb1tm1Kri/J
Research Applications
This mouse can be used to support research in many areas including:
Apoetm1Unc relatedCardiovascular Research
Heart Abnormalities
Scarb1tm1Kri relatedCardiovascular Research
Atherosclerosis
Hypercholesterolemia
Mouse/Human Gene Homologs
Alzheimer's
hyperlipoproteinemia, type III
Cardiovascular Research
Hypercholesterolemia
Metabolism Research
Lipid Metabolism
Reproductive Biology Research
Fertility Defects (females only)
References
Selected Reference(s)
Additional ReferencesBraun A; Trigatti BL; Post MJ; Sato K; Simons M; Edelberg JM; Rosenberg RD; Schrenzel M; Krieger M. 2002. Loss of SR-BI expression leads to the early onset of occlusive atherosclerotic coronary artery disease, spontaneous myocardial infarctions, severe cardiac dysfunction, and premature death in apolipoprotein E-deficient mice. Circ Res 90(3):270-6. [PubMed: 11861414] [MGI Ref ID J:109751]
Price and Supply Information
| Strain Name: | B6;129-Scarb1tm1Kri Apoetm1Unc/J |
| Stock Number: | 004362 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | |||||
|---|---|---|---|---|---|
| Cryorecovery Fee | $2470.00 | ||||
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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