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Strain Name:

FVB.Cg-Tg(MMTV-vHaras)SH1Led/J

Stock Number:

004363

Availability:

Repository- Live

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Former Name      FVB.Cg-Tg(MMTV-HRAS)1Led/J    (Changed: 15-DEC-04 )
      MMTV/v-Ha-ras    (Changed: 15-DEC-04 )
      TG.SH    (Changed: 15-DEC-04 )
Genes & Alleles   MMTV;   Tg(MMTV-vHaras)SH1Led;   vHa-ras;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating SystemInbred x Hemizygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Philip Leder,   Harvard Medical School
GenerationN?+9 (05-DEC-07)

Strain Description
These transgenic mice express the Harvey RAS (human) gene under the direction of the mouse mammary tumor virus promoter. The majority of expression of the transgene is detected in the mammary tissue and salivary glands. Both male and female transgenic mice develop malignant lymphoid tissue and mammary and salivary gland tumors as early as 5 weeks of age. Half of the female transgenic animals develop tumors by 6 months of age. Histological analysis of mammary tumors revealed most of the tumors were adenocarcinomas and were locally invasive with some cases of metastasis to liver and/or lung. The tumors were found to express the transgene transcript. Protein-tyrosine phosphatase epsilon mRNA and protein was found to be highly expressed only in mammary tumors. In 20% of the transgenic mice diffuse benign hyperplasia develops in the Harderian lacrimal gland causing bilateral exophthalmia. The donating investigator reports hemizygous females develop tumors by delivery of their first litter (i.e. tumorigenesis is accelerated by pregnancy); and hemizygous males develop large salivary gland tumors in the neck and eye opacity by 5 to 6 months of age. The donating investigator has found hemizygous males to have low sperm counts and high incidences of sperm abnormalities resulting in low fertility. This transgenic mouse strain represents a model that may be useful in studies of mammary carcinoma.

Strain Development
A transgenic construct, pA9 chimeric plasmid, containing the p21 structural gene of Harvey murine sarcoma virus (Ha-MuSV) and the MMTV promoter was injected into fertilized B6;CD-1 mouse eggs. Transgenic mice were later backcrossed to FVB/N.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(MMTV-vHaras)SH1Led/Tg(MMTV-vHaras)SH1Led

        involves: 129X1/SvJ * C57BL/6J * CD-1 * FVB
  • tumorigenesis
  • carcinoma (MGI Ref ID J:86074)
    • developed tumors of various sizes that corresponded to moderately to poorly differentiated invasive duct carcinomas with most tumors growing as solid areas that contained no stroma
    • some of the largest tumors were infiltrated by hemorrhage, resulting in a microcystic growth pattern
    • mammary adenocarcinoma (MGI Ref ID J:86074)
      • developed mammary gland tumors between 20 to 45 weeks of age with the mean age for onset at 25 weeks
  • increased metastatic potential (MGI Ref ID J:86074)
    • 31% developed lung metastases

Gene & Allele Details

Allele Symbol Tg(MMTV-vHaras)SH1Led
Allele Name transgene insertion SH1, Philip Leder
Common Name(s) MMTV-H-Ras; MMTV-v-Ha-ras; MMTV/v-Ha-ras; TG.SH; Tg(MMTV-HRAS)1Led;
Mutation Made By Philip Leder,   Harvard Medical School
Strain of Origin(CD-1 x C57BL/6J)F1
Expressed Gene vHa-ras, Harvey rat sarcoma viral oncogene homolog,
Promoter MMTV, Mouse Mammary Tumor Virus, MMTV
General Note Multiple transgenic lines were produced.

Both male and female transgenic mice develop malignant lymphoid tissue and mammary and salivary gland tumors as early as 5 weeks of age. Half of the female transgenic animals develop tumors by 6 months of age. Histological analysis of mammary tumors revealed most of the tumors were adenocarcinomas and were locally invasive with some cases of metastasis to liver and/or lung. The tumors were found to express the transgene transcript. Protein-tyrosine phosphatase epsilon mRNA and protein was found to be highly expressed only in mammary tumors. In 20% of the transgenic mice diffuse benign hyperplasia develops in the Harderian lacrimal gland causing bilateral exophthalmia. Hemizygous transgenic female mice develop tumors by delivery time of first litter with tumorigenesis accelerated by pregnancy. Hemizygous transgenic male mice develop large salivary gland tumors in the neck and eye opacity by 5 to 6 months of age. Hemizygous males have low sperm counts and high incidence of sperm abnormalities resulting in low fertility.

Molecular Note This transgene contains the MMTV (mouse mammary tumor virus) promoter and an allele of the Harvey rat sarcoma viral oncogene (viral) carrying two activating point mutations: one in codon 12, Gly12Arg, and one in codon 59, Ala59Thr. The transgenic construct was reported to be highly expressed in the mammary glands and salivary glands, expressed in the Harderian glands, thymus and spleen, slightly expressed in the lungs, ovaries and kidneys, and little or no expression was reported to have been observed inthe liver and skeletal muscle of these mice. [MGI Ref ID J:45456] [MGI Ref ID J:46454] [MGI Ref ID J:90327]

Control Information

  Control
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Genotyping Protocols

FVB.Cg-Tg(MMTV-HRAS)1Led

Colony Maintenance

Breeding & HusbandryThe strain is maintained by hemizygous female X wildtype FVB/N male matings. Only 10% of hemizygous males are fertile.
Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying other alleles of MMTV     (17 strains)

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation
Increased Tumor Incidence (Mammary Gland Tumors)
Other (tumor metastasis)

References

Selected Reference(s)

Sinn E; Muller W; Pattengale P; Tepler I; Wallace R; Leder P. 1987. Coexpression of MMTV/v-Ha-ras and MMTV/c-myc genes in transgenic mice: synergistic action of oncogenes in vivo. Cell 49(4):465-75. [PubMed: 3032456]  [MGI Ref ID J:46454]

Additional References

Price and Supply Information

Strain Name: FVB.Cg-Tg(MMTV-vHaras)SH1Led/J
Stock Number: 004363

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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