Strain Name:

B6.129X1-Brs3tm1Jfb/J

Stock Number:

004366

Availability:

Repository-Cryopreserved

Use Restrictions Apply, see Terms of Use

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
 
Donating Investigator James Battey, Jr.,   National Institutes of Health

Description
Mice that are homozygous for the targeted mutation are viable, normal in size at birth and do not display any gross physical or behavioral abnormalities. This targeted mutation is X-linked; males bearing the targeted allele display a mutant phenotype. At 15 to 16 weeks of age male mice heterozygous for the mutant allele display increased body weight as compared to wildtype littermates. This mutant mouse strain represents a model that may be useful in studies related to energy metabolism and obesity.

Development
A targeting vector containing neomycin resistance and thymidine kinase genes was used to disrupt exon 2 of the targeted allele. The construct was electroporated into 129X1/SvJ derived Gsi1 embryonic stem (ES) cells. The resulting chimeric animals were backcrossed to C57BL/6 mice.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Brs3tm1Jfb/Brs3tm1Jfb

        B6.129X1-Brs3tm1Jfb
  • growth/size phenotype
  • increased body weight (MGI Ref ID J:134021)
    • mutants fed ad lib show significantly greater body weights than wild-type mice fed ad lib starting at 13 weeks of age and continuing throughout the experiment duration; by 32 weeks, mutant animals displayed 28% greater body weight than controls
    • pair-feeding of mutant animals to match caloric intake of ad lib-fed wild-type normalized body weights of mutants so that pair-fed animal weights are similar to ad lib-fed wild-type mice
    • at 10 weeks of age, pair-fed mutants are significantly heavier than wild-type mice
  • adipose tissue phenotype
  • *normal* adipose tissue phenotype (MGI Ref ID J:134021)
    • increased weight at 10 weeks of age of pair-fed mutants does not correlate to increased brown adipose tissue compared to wild-type
    • abnormal epididymal fat pad morphology (MGI Ref ID J:134021)
    • increased adipose tissue amount (MGI Ref ID J:134021)
      • increased weight at 10 weeks of age of pair-fed mutants is accompanied by significant increases in subcutaneous and epididymal fat
      • at 36 weeks of age, mutants have significantly more subcutaneous and epididymal fat than wild-type mice
    • increased brown adipose tissue amount (MGI Ref ID J:134021)
      • at 36 weeks of age, mutants have significantly more brown fat than wild-type mice
  • homeostasis/metabolism phenotype
  • increased circulating insulin level (MGI Ref ID J:134021)
    • ad lib-fed mutants show significantly elevated insulin levels relative to ad lib fed wild-type mice
    • pair-fed mutants showed normalized insulin levels to levels in ad lib-fed wild-type mice
  • increased circulating leptin level (MGI Ref ID J:134021)
    • at 10 weeks of age, pair-fed mutants have elevated plasma leptin levels compared to wild-type animals
  • behavior/neurological phenotype
  • abnormal food intake (MGI Ref ID J:134021)
    • ad lib-fed mutants show a decrease in grams of food consumed as percentage of body weight compared to wild-type at 20 and 28 weeks of age; less food is consumed relative to body weight
    • increased eating behavior (MGI Ref ID J:134021)
      • over course of experiment, mutants fed ad lib show a higher food intake than ad lib-fed wild-type
      • number of food pellets consumed in 24 hours greater in ad lib-fed mutants; number of meals taken in 24 hours is not different from ad lib-fed wild-type mice but meal size is significantly greater
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Hyperinsulinemia (adult onset)
Obesity Without Diabetes

Endocrine Deficiency Research
Hypothalamus/Pituitary Defects

Internal/Organ Research
Gastrointestinal Defects

Metabolism Research
Lipid Metabolism

Neurobiology Research
Behavioral and Learning Defects
Metabolic Defects
Receptor Defects

Research Tools
Neurobiology Research

Genes & Alleles

Gene & Allele Information

Allele Symbol Brs3tm1Jfb
Allele Name targeted mutation 1, James F Battey
Allele Type Targeted (knock-out)
Common Name(s) BRS-3 KO;
Strain of Origin129X1/SvJ
ES Cell Line NameGSI-1
ES Cell Line Strain129X1/SvJ
Gene Symbol and Name Brs3, bombesin-like receptor 3
Chromosome X
Gene Common Name(s) BRS-3;
Molecular Note Exon 2 has been replaced with a neomycin resistance cassette. No transcript from the disrupted gene was detected in mRNA from brains of homozygous mutant mice by reverse transcription followed by PCR analysis (RT-PCR). [MGI Ref ID J:134021]

Genotyping

Genotyping Information

Genotyping Protocols

Brs3tm1Jfb, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Ladenheim EE; Hamilton NL; Behles RR; Bi S; Hampton LL; Battey JF; Moran TH. 2008. Factors contributing to obesity in bombesin receptor subtype-3-deficient mice. Endocrinology 149(3):971-8. [PubMed: 18039774]  [MGI Ref ID J:134021]

Additional References

Weber HC; Hampton LL; Jensen RT; Battey JF. 1998. Structure and chromosomal localization of the mouse bombesin receptor subtype 3 gene. Gene 211(1):125-31. [PubMed: 9573346]  [MGI Ref ID J:47585]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryThis strain originated on a 129 background, has been backcrossed for 9 generations on the C57BL/6J background. When held in a live colony, breeder pairs were het female X hemi male.
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


See Terms of Use


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Contact Information
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Fax: 207.288.6150
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
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Contact information

General inquiries

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phone:207-288-6470
fax:207-288-6655

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“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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