Strain Name:

B6.129-Adra2atm1Bkk/J

Stock Number:

004367

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating InvestigatorDr. Brian Kobilka,   Stanford University

Description
Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. Expression of the receptor in the brain was significantly reduced as assessed by antagonist binding, which indicated a 90% reduction. The antagonist binding assay is not entirely specific for the receptor, as the residual 10% binding was attributable to a different subtype of receptor. Mutant mice exhibit a resting tachycardia of more than 180 beats/min greater than normal. Although mutant mice do not have elevated blood pressure, administration of the hypotensive agonist, dexmedetomidine, does not reduce blood pressure. Electrical stimulation of isolated heart atria at frequencies mimicking physiological sympathetic nerve activity results in increased release of noradrenaline at high frequencies and inhibited release at low frequencies. Noradrenaline release from isolated wildtype atria is higher at high frequencies than at low frequencies. Inhibition of neurogenic contraction response to electrical stimulation in isolated vas deferens by dexmedetomidine is reduced in the mutant.

Development
A targeting vector containing neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter and herpes simplex virus thymidine kinase gene was used to disrupt the sequence of the targeted gene encoding the third transmembrane domain. The construct was electroporated into 129X1/SvJ x 129S1/Sv derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were aggregated with 8-cell FVB/N morulae. The resulting chimeric animals were backcrossed to C57BL/6 mice.

Related Strains

Strains carrying other alleles of Adra2a
002777   B6.129S2-Adra2atm1Lel/J
View Strains carrying other alleles of Adra2a     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Adra2atm1Bkk/Adra2atm1Bkk

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • cardiovascular system phenotype
  • abnormal systemic arterial blood pressure
    • unlike wild-type controls, administration of the hypotensive agonist, dexmedetomidine, did not reduce blood pressure   (MGI Ref ID J:76317)
  • increased heart rate
    • resting heart rate more than 180 beats/min greater than normal   (MGI Ref ID J:76317)
  • nervous system phenotype
  • abnormal synaptic norepinephrine release
    • increased release at high frequency electrical stimulation   (MGI Ref ID J:58591)
    • decreased release at low frequency electrical stimulation   (MGI Ref ID J:58591)

Adra2atm1Bkk/Adra2atm1Bkk

        involves: 129S1/Sv * 129X1/SvJ
  • homeostasis/metabolism phenotype
  • abnormal glucose homeostasis
    • unlike in wild-type mice, hyperglycemia cannot be induced by treatment with 3-iodothyronamine and/or 6-OH dopamine   (MGI Ref ID J:130781)
    • hypoglycemia
      • following treatment with 3-iodothyronamine   (MGI Ref ID J:130781)

Adra2atm1Bkk/Adra2atm1Bkk

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
  • behavior/neurological phenotype
  • abnormal spike wave discharge
    • mice treated with epinephrine fail to reduce burst frequency of induced epileptiform activity unlike in control mice   (MGI Ref ID J:197965)
  • nervous system phenotype
  • abnormal spike wave discharge
    • mice treated with epinephrine fail to reduce burst frequency of induced epileptiform activity unlike in control mice   (MGI Ref ID J:197965)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Adra2atm1Bkk related

Cardiovascular Research
Heart Abnormalities
      tachycardia
Hypotension

Neurobiology Research
Epilepsy

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Adra2atm1Bkk
Allele Name targeted mutation 1, Brian K Kobilka
Allele Type Targeted (Null/Knockout)
Common Name(s) alpha2A-; alpha2A-AR-; alpha2A-ARKO; alpha2A-KO;
Mutation Made ByDr. Brian Kobilka,   Stanford University
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Adra2a, adrenergic receptor, alpha 2a
Chromosome 19
Gene Common Name(s) ADRA2; ADRA2R; ADRAR; ALPHA2AAR; AW122659; Adra-2; Adra-2a; CA2-47; RATRG20; RG20; ZNF32; adrenergic receptor, alpha 2; alpha(2A)AR; alpha2A; alpha2A-AR; alpha2A-adrenergic receptor; expressed sequence AW122659;
Molecular Note A neomycin cassette inserted into the gene by homologous recombination resulted in a premature stop codon within the third transmembrane domain. Confirmation of the targeting event was demonstrated through Southern analysis. [MGI Ref ID J:76317]

Genotyping

Genotyping Information

Genotyping Protocols

Adra2atm1Bkk, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Altman JD; Trendelenburg AU; MacMillan L; Bernstein D; Limbird L; Starke K; Kobilka BK; Hein L. 1999. Abnormal regulation of the sympathetic nervous system in alpha2A-adrenergic receptor knockout mice. Mol Pharmacol 56(1):154-61. [PubMed: 10385696]  [MGI Ref ID J:76317]

Additional References

Hein L; Altman JD; Kobilka BK. 1999. Two functionally distinct alpha2-adrenergic receptors regulate sympathetic neurotransmission. Nature 402(6758):181-4. [PubMed: 10647009]  [MGI Ref ID J:58591]

Adra2atm1Bkk related

Bonaventure P; Nepomuceno D; Hein L; Sutcliffe JG; Lovenberg T; Hedlund PB. 2004. Radioligand binding analysis of knockout mice reveals 5-hydroxytryptamine(7) receptor distribution and uncovers 8-hydroxy-2-(di-n-propylamino)tetralin interaction with alpha(2) adrenergic receptors. Neuroscience 124(4):901-11. [PubMed: 15026130]  [MGI Ref ID J:89990]

Brede M; Nagy G; Philipp M; Sorensen JB; Lohse MJ; Hein L. 2003. Differential control of adrenal and sympathetic catecholamine release by alpha 2-adrenoceptor subtypes. Mol Endocrinol 17(8):1640-6. [PubMed: 12764077]  [MGI Ref ID J:126731]

Brum PC; Kosek J; Patterson A; Bernstein D; Kobilka B. 2002. Abnormal cardiac function associated with sympathetic nervous system hyperactivity in mice. Am J Physiol Heart Circ Physiol 283(5):H1838-45. [PubMed: 12384461]  [MGI Ref ID J:108048]

Bucheler MM; Hadamek K; Hein L. 2002. Two alpha(2)-adrenergic receptor subtypes, alpha(2A) and alpha(2C), inhibit transmitter release in the brain of gene-targeted mice. Neuroscience 109(4):819-26. [PubMed: 11927164]  [MGI Ref ID J:126644]

Bueno CR Jr; Ferreira JC; Pereira MG; Bacurau AV; Brum PC. 2010. Aerobic exercise training improves skeletal muscle function and Ca2+ handling-related protein expression in sympathetic hyperactivity-induced heart failure. J Appl Physiol 109(3):702-9. [PubMed: 20595538]  [MGI Ref ID J:185923]

Davies MF; Tsui JY; Flannery JA; Li X; DeLorey TM; Hoffman BB. 2003. Augmentation of the noradrenergic system in alpha-2 adrenergic receptor deficient mice: anatomical changes associated with enhanced fear memory. Brain Res 986(1-2):157-65. [PubMed: 12965240]  [MGI Ref ID J:107779]

Fagerholm V; Gronroos T; Marjamaki P; Viljanen T; Scheinin M; Haaparanta M. 2004. Altered glucose homeostasis in alpha2A-adrenoceptor knockout mice. Eur J Pharmacol 505(1-3):243-52. [PubMed: 15556159]  [MGI Ref ID J:101927]

Ferreira JC; Bacurau AV; Evangelista FS; Coelho MA; Oliveira EM; Casarini DE; Krieger JE; Brum PC. 2008. The role of local and systemic renin angiotensin system activation in a genetic model of sympathetic hyperactivity-induced heart failure in mice. Am J Physiol Regul Integr Comp Physiol 294(1):R26-32. [PubMed: 17977919]  [MGI Ref ID J:130320]

Goldenstein BL; Nelson BW; Xu K; Luger EJ; Pribula JA; Wald JM; O'Shea LA; Weinshenker D; Charbeneau RA; Huang X; Neubig RR; Doze VA. 2009. Regulator of G protein signaling protein suppression of Galphao protein-mediated alpha2A adrenergic receptor inhibition of mouse hippocampal CA3 epileptiform activity. Mol Pharmacol 75(5):1222-30. [PubMed: 19225179]  [MGI Ref ID J:197965]

Hein L; Altman JD; Kobilka BK. 1999. Two functionally distinct alpha2-adrenergic receptors regulate sympathetic neurotransmission. Nature 402(6758):181-4. [PubMed: 10647009]  [MGI Ref ID J:58591]

Hinoi E; Gao N; Jung DY; Yadav V; Yoshizawa T; Myers MG Jr; Chua SC Jr; Kim JK; Kaestner KH; Karsenty G. 2008. The sympathetic tone mediates leptin's inhibition of insulin secretion by modulating osteocalcin bioactivity. J Cell Biol 183(7):1235-42. [PubMed: 19103808]  [MGI Ref ID J:145998]

Hocker J; Gruenewald M; Meybohm P; Schaper C; Scholz J; Steinfath M; Bein B. 2010. Nefopam but not physostigmine affects the thermoregulatory response in mice via alpha(2)-adrenoceptors. Neuropharmacology 58(2):495-500. [PubMed: 19744502]  [MGI Ref ID J:179564]

Ihalainen JA; Tanila H. 2004. In vivo regulation of dopamine and noradrenaline release by alpha2A-adrenoceptors in the mouse nucleus accumbens. J Neurochem 91(1):49-56. [PubMed: 15379886]  [MGI Ref ID J:93277]

Ihalainen JA; Tanila H. 2002. In vivo regulation of dopamine and noradrenaline release by alpha2A-adrenoceptors in the mouse prefrontal cortex. Eur J Neurosci 15(11):1789-94. [PubMed: 12081658]  [MGI Ref ID J:108075]

Juhila J; Honkanen A; Sallinen J; Haapalinna A; Korpi ER; Scheinin M. 2005. alpha(2A)-Adrenoceptors regulate d-amphetamine-induced hyperactivity and behavioural sensitization in mice. Eur J Pharmacol 517(1-2):74-83. [PubMed: 15978573]  [MGI Ref ID J:103792]

Knaus A; Zong X; Beetz N; Jahns R; Lohse MJ; Biel M; Hein L. 2007. Direct inhibition of cardiac hyperpolarization-activated cyclic nucleotide-gated pacemaker channels by clonidine. Circulation 115(7):872-80. [PubMed: 17261653]  [MGI Ref ID J:132333]

Lahdesmaki J; Sallinen J; MacDonald E; Kobilka BK; Fagerholm V; Scheinin M. 2002. Behavioral and neurochemical characterization of alpha(2A)-adrenergic receptor knockout mice. Neuroscience 113(2):289-99. [PubMed: 12127086]  [MGI Ref ID J:109288]

Lahdesmaki J; Sallinen J; MacDonald E; Sirvio J; Scheinin M. 2003. Alpha2-adrenergic drug effects on brain monoamines, locomotion, and body temperature are largely abolished in mice lacking the alpha2A-adrenoceptor subtype. Neuropharmacology 44(7):882-92. [PubMed: 12726820]  [MGI Ref ID J:97152]

Lahdesmaki J; Scheinin M; Pertovaara A; Mansikka H. 2003. The alpha2A-adrenoceptor subtype is not involved in inflammatory hyperalgesia or morphine-induced antinociception. Eur J Pharmacol 468(3):183-9. [PubMed: 12754056]  [MGI Ref ID J:102597]

Makaritsis KP; Johns C; Gavras I; Altman JD; Handy DE; Bresnahan MR; Gavras H. 1999. Sympathoinhibitory function of the alpha(2A)-adrenergic receptor subtype. Hypertension 34(3):403-7. [PubMed: 10489385]  [MGI Ref ID J:59489]

Mansikka H; Lahdesmaki J; Scheinin M; Pertovaara A. 2004. Alpha(2A) adrenoceptors contribute to feedback inhibition of capsaicin-induced hyperalgesia. Anesthesiology 101(1):185-90. [PubMed: 15220790]  [MGI Ref ID J:105328]

Medeiros A; Rolim NP; Oliveira RS; Rosa KT; Mattos KC; Casarini DE; Irigoyen MC; Krieger EM; Krieger JE; Negrao CE; Brum PC. 2008. Exercise training delays cardiac dysfunction and prevents calcium handling abnormalities in sympathetic hyperactivity-induced heart failure mice. J Appl Physiol 104(1):103-9. [PubMed: 17975126]  [MGI Ref ID J:147520]

Moura E; Afonso J; Hein L; Vieira-Coelho MA. 2006. Alpha2-adrenoceptor subtypes involved in the regulation of catecholamine release from the adrenal medulla of mice. Br J Pharmacol 149(8):1049-58. [PubMed: 17075569]  [MGI Ref ID J:135922]

Muthig V; Gilsbach R; Haubold M; Philipp M; Ivacevic T; Gessler M; Hein L. 2007. Upregulation of soluble vascular endothelial growth factor receptor 1 contributes to angiogenesis defects in the placenta of alpha 2B-adrenoceptor deficient mice. Circ Res 101(7):682-91. [PubMed: 17673674]  [MGI Ref ID J:141347]

Niederhoffer N; Hein L; Starke K. 2004. Modulation of the baroreceptor reflex by alpha 2A-adrenoceptors: a study in alpha 2A knockout mice. Br J Pharmacol 141(5):851-9. [PubMed: 14769782]  [MGI Ref ID J:102353]

Oliveira RS; Ferreira JC; Gomes ER; Paixao NA; Rolim NP; Medeiros A; Guatimosim S; Brum PC. 2009. Cardiac anti-remodelling effect of aerobic training is associated with a reduction in the calcineurin/NFAT signalling pathway in heart failure mice. J Physiol 587(Pt 15):3899-910. [PubMed: 19505981]  [MGI Ref ID J:176793]

Ozdogan UK; Lahdesmaki J; Hakala K; Scheinin M. 2004. The involvement of alpha 2A-adrenoceptors in morphine analgesia, tolerance and withdrawal in mice. Eur J Pharmacol 497(2):161-71. [PubMed: 15306201]  [MGI Ref ID J:101972]

Ozdogan UK; Lahdesmaki J; Mansikka H; Scheinin M. 2004. Loss of amitriptyline analgesia in alpha 2A-adrenoceptor deficient mice. Eur J Pharmacol 485(1-3):193-6. [PubMed: 14757140]  [MGI Ref ID J:103497]

Ozdogan UK; Lahdesmaki J; Scheinin M. 2006. The analgesic efficacy of partial opioid agonists is increased in mice with targeted inactivation of the alpha2A-adrenoceptor gene. Eur J Pharmacol 529(1-3):105-13. [PubMed: 16325800]  [MGI Ref ID J:106379]

Paris A; Mantz J; Tonner PH; Hein L; Brede M; Gressens P. 2006. The effects of dexmedetomidine on perinatal excitotoxic brain injury are mediated by the alpha2A-adrenoceptor subtype. Anesth Analg 102(2):456-61. [PubMed: 16428542]  [MGI Ref ID J:134285]

Paris A; Philipp M; Tonner PH; Steinfath M; Lohse M; Scholz J; Hein L. 2003. Activation of alpha 2B-adrenoceptors mediates the cardiovascular effects of etomidate. Anesthesiology 99(4):889-95. [PubMed: 14508322]  [MGI Ref ID J:106006]

Philipp M; Brede ME; Hadamek K; Gessler M; Lohse MJ; Hein L. 2002. Placental alpha(2)-adrenoceptors control vascular development at the interface between mother and embryo. Nat Genet 31(3):311-5. [PubMed: 12068299]  [MGI Ref ID J:77486]

Pozgajova M; Sachs UJ; Hein L; Nieswandt B. 2006. Reduced thrombus stability in mice lacking the alpha2A-adrenergic receptor. Blood 108(2):510-4. [PubMed: 16507775]  [MGI Ref ID J:135683]

Regard JB; Kataoka H; Cano DA; Camerer E; Yin L; Zheng YW; Scanlan TS; Hebrok M; Coughlin SR. 2007. Probing cell type-specific functions of Gi in vivo identifies GPCR regulators of insulin secretion. J Clin Invest 117(12):4034-43. [PubMed: 17992256]  [MGI Ref ID J:130781]

Riccio O; Hurni N; Murthy S; Vutskits L; Hein L; Dayer A. 2012. Alpha2-adrenergic receptor activation regulates cortical interneuron migration. Eur J Neurosci 36(7):2879-87. [PubMed: 22805283]  [MGI Ref ID J:207652]

Rolim NP; Medeiros A; Rosa KT; Mattos KC; Irigoyen MC; Krieger EM; Krieger JE; Negrao CE; Brum PC. 2007. Exercise training improves the net balance of cardiac Ca2+ handling protein expression in heart failure. Physiol Genomics 29(3):246-52. [PubMed: 17244791]  [MGI Ref ID J:128677]

Savontaus E; Fagerholm V; Rahkonen O; Scheinin M. 2008. Reduced blood glucose levels, increased insulin levels and improved glucose tolerance in alpha2A-adrenoceptor knockout mice. Eur J Pharmacol 578(2-3):359-64. [PubMed: 17964569]  [MGI Ref ID J:141464]

Sawamura S; Kingery WS; Davies MF; Agashe GS; Clark JD; Kobilka BK; Hashimoto T; Maze M. 2000. Antinociceptive action of nitrous oxide is mediated by stimulation of noradrenergic neurons in the brainstem and activation of [alpha]2B adrenoceptors. J Neurosci 20(24):9242-51. [PubMed: 11125002]  [MGI Ref ID J:120560]

Scheibner J; Trendelenburg AU; Hein L; Starke K. 2001. Alpha2-adrenoceptors modulating neuronal serotonin release: a study in alpha2-adrenoceptor subtype-deficient mice. Br J Pharmacol 132(4):925-33. [PubMed: 11181434]  [MGI Ref ID J:115396]

Scheibner J; Trendelenburg AU; Hein L; Starke K; Blandizzi C. 2002. Alpha 2-adrenoceptors in the enteric nervous system: a study in alpha 2A-adrenoceptor-deficient mice. Br J Pharmacol 135(3):697-704. [PubMed: 11834617]  [MGI Ref ID J:103275]

Tan CM; Wilson MH; MacMillan LB; Kobilka BK; Limbird LE. 2002. Heterozygous alpha 2A-adrenergic receptor mice unveil unique therapeutic benefits of partial agonists. Proc Natl Acad Sci U S A 99(19):12471-6. [PubMed: 12205290]  [MGI Ref ID J:127138]

Trendelenburg AU; Hein L; Gaiser EG; Starke K. 1999. Occurrence, pharmacology and function of presynaptic alpha2-autoreceptors in alpha2A/D-adrenoceptor-deficient mice Naunyn Schmiedebergs Arch Pharmacol 360(5):540-51. [PubMed: 10598793]  [MGI Ref ID J:59811]

Vanzelli AS; Medeiros A; Rolim N; Bartholomeu JB; Cunha TF; Bechara LR; Gomes ER; Mattos KC; Sirvente R; Salemi VM; Mady C; Negrao CE; Guatimosim S; Brum PC. 2013. Integrative effect of carvedilol and aerobic exercise training therapies on improving cardiac contractility and remodeling in heart failure mice. PLoS One 8(5):e62452. [PubMed: 23658728]  [MGI Ref ID J:202182]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain originated on a FVB;129 background, has been backcrossed for 5 generations to C57BL/6 and may be maintained as a homozygote. Donating Investigator reports mutant mice usually have less than 6 litters and less than 6 pups per litter.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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