Strain Name: |
B6.Cg-Rag1tm1Mom Ins2Akita/J |
|---|---|
Stock Number: |
004369 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
C57BL/6J-Rag1tm1MomIns2Akita/J (Changed: 15-DEC-04
) |
| Genes & Alleles | Ins2; Ins2Akita; Rag1; Rag1tm1Mom; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Spontaneous Mutation Type JAX® GEMM® Strain - Targeted Mutation Mating System See Colony Maintenance (Female x Male) Species laboratory mouse Donating Investigator Leonard Shultz, The Jackson Laboratory Generation [N2+F4p]+F2 (08-JAN-08) Strain Description
Mice homozygous for the Rag1 targeted mutation and heterozygous for the Akita spontaneous display the diabetes phenotype in the absence of B and T cells and unlike single Akita mice, double mutants do not reject allografts. Mice heterozygous for only the Akita spontaneous mutation are viable and fertile. (Homozygotes typically die by 12 weeks of age from extreme hyperglycemia.) Symptoms in heterozygous mutant mice include hyperglycemia, hypoinsulinemia, polydipsia, and polyuria, beginning at approximately 3-4 weeks of age. The diabetic phenotype is more severe and progressive in heterozygous males than in females. Obesity and insulitis do not accompany diabetes.This double mutant strain is ideally suited for use in allogeneic or xenogeneic islet or stem cell transplantation protocols because the mice are severely immunocompromised and spontaneously develop diabetes at a young age.
Gene & Allele Details
| Allele Symbol | Ins2Akita | ||
|---|---|---|---|
| Allele Name | Akita | ||
| Common Name(s) | Ins2C96Y; Ins2Mody; Mody; Mody4; | ||
| Strain of Origin | C57BL/6 | ||
| Gene Symbol and Name | Ins2, insulin II | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | AA986540; Ins-2; InsII; Mody; Mody4; expressed sequence AA986540; maturity onset diabetes of the young; maturity onset diabetes of the young 4; | ||
| Molecular Note | In the mutant allele a transition from G to A at nucleotide 1907 disrupted an Fnu4HI site in exon 3. This mutation changed the seventh amino acid in the A chain of mature insulin, Cys96 (TGC), to Tyr (TAC). The authors predict that the transition would disrupt a disulfide bond between the A and the B chains and would likely induce a major conformational change in insulin 2 molecules. RT-PCR studies suggest that both normal and mutant Ins2 alleles are transcribed similarly in pancreatic islets of heterozygous mice, although immunofluorescence and immunoblot analyses of heterozygous islets detected reduced levels of insulin and proinsulin. [MGI Ref ID J:51935] | ||
| Allele Symbol | Rag1tm1Mom | ||
| Allele Name | targeted mutation 1, Peter Mombaerts | ||
| Common Name(s) | RAG-1-; RAG1null; Rag-; Rag-1KO; Rag1-; | ||
| Mutation Made By | Peter Mombaerts, Max Planck Institute of Biophysics | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt1<+> | ||
| ES Cell Line Name | AB1 | ||
| ES Cell Line Strain | 129S7/SvEvBrd-Hprt1<+> | ||
| Gene Symbol and Name | Rag1, recombination activating gene 1 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | MGC43321; RNF74; Rag-1; | ||
| Molecular Note | A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice. [MGI Ref ID J:1934] [MGI Ref ID J:96036] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 003548 C57BL/6-Ins2Akita/J | ||
| 000664 C57BL/6J | ||
| Appropriate controls depend on the nature of the experiment. C57BL/6J mice or C57BL/6-Ins2Akita may be used as controls. Alternatively, mice from the colony that are wild-type for the Ins2Akita mutation but homozygous for the Rag1tm1Mom mutation may also be appropriate. | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Ins2Akita
Rag1tm1Mom
Colony Maintenance
| Breeding & Husbandry | This strain is maintained by breeding wildtype females (Rag1-/-Ins2+/+) to affected (hyperglycemic) males (Rag1-/-Ins2Akita/+). The Rag1tm1Mom allele is maintained in homozygosity and the Ins2Akita allele is maintained in heterozygosity. After onset of diabetes, when cages become very wet due to polyuria, the health of Ins2Akita heterozygotes is best maintained by housing them individually in cages containing a mixture of regular litter and Alpha-Dri, changed twice weekly. Specific pathogen free (SPF) conditions are recommended. Expected coat color is Black. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Ins2Akita allele
006860 B6.129-Ins2Akita Bdkrb2tm1Jfh/SmiJ 006580 B6.Cg-Ins2Akita Ldlrtm1Her/J 003548 C57BL/6-Ins2Akita/J 007562 D2.B6-Ins2Akita/MatbJ 006867 FVB.B6-Ins2Akita/MlnJ View Strains carrying Ins2Akita (5 strains)
002194 B6;CBA-Tg(CD3E)26Cpt/J 002506 STOCK Tg(CD3E)26Cpt-Rag1tm1Mom/J
Additional Web Information
Congenic Nomenclature
Animal Health Reports
Room Number FGB29
Research Applications
This mouse can be used to support research in many areas including:
Ins2 relatedResearch Tools
Immunology and Inflammation Research (B and T cell deficiency)
Ins2Akita relatedDiabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)
Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
Rag1tm1Mom relatedCell Biology Research
Protein Processing
Diabetes and Obesity Research
Hyperglycemia
Hypoinsulinemia
Impaired Insulin Processing
Insulin Receptors and Growth Factors
Islet Transplantation Studies
Type 1 Diabetes (IDDM) (MODY, mature onset diabetes of the young)
Endocrine Deficiency Research
Pancreas Defects
Cancer Research
Toxicology (B and T cell deficiency) (xenograft/transplant host)
Hematological Research
Immunological Defects (B and T cell deficiency)
Immunology and Inflammation Research
Immunodeficiency (B and T cell deficiency)
Inflammation (B and T cell deficiency)
T Cell Receptor Signaling Defects (B and T cell deficiency)
Internal/Organ Research
Lymphoid Tissue Defects (B and T cell deficiency)
Research Tools
Cancer Research (B and T cell deficiency) (xenograft/transplant host)
Toxicology Research (xenograft/transplant host)
References
Selected Reference(s)
Additional ReferencesMathews CE; Langley SH; Leiter EH. 2002. New mouse model to study islet transplantation in insulin-dependent diabetes mellitus. Transplantation 73(8):1333-6. [PubMed: 11981430] [MGI Ref ID J:76224]
Price and Supply Information
| Strain Name: | B6.Cg-Rag1tm1Mom Ins2Akita/J |
| Stock Number: | 004369 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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