Strain Name:

B6.129-Ptger2tm1Brey/J

Stock Number:

004376

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Richard M. Breyer,   Vanderbilt University

Description
Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. Reduced fertility in homozygous females is due to a pre-implantation defect. Fewer eggs are released during ovulation, of which fewer are fertilized and implanted when compared to wildtype controls, resulting in smaller litters (3 pups/litter). Mutant mice display slightly elevated baseline systolic blood pressure. Prostaglandin 2 infusion or high salt diet causes systolic hypertension in homozygous mice. This mutant mouse strain represents a model that may be useful in studies of salt sensitive hypertension and infertility.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes driven by the mouse phosphoglycerate kinase promoter was used to disrupt sequence of the targeted gene encoding the N-terminal of the protein. The construct was electroporated into 129S6/SvEv derived TL-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were backcrossed to C57BL/6 females. Simple sequence length polymorphism (SSLP) analysis confirmed that there are no chromosomal segments derived from 129, except for regions immediately flanking the disrupted locus, proximal chromosome 14.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Asthma, Nasal Polyps, and Aspirin Intolerance   (PTGER2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Ptger2tm1Brey/Ptger2tm1Brey

        B6.129S6-Ptger2tm1Brey
  • homeostasis/metabolism phenotype
  • abnormal response to injury
    • after middle cerebral artery occlusion induced infarction cerebral blood flow in the non-ischemic contralateral hemisphere is increased compared to wild-type controls   (MGI Ref ID J:87388)
    • increased cerebral infarction size
      • following 90 min of ischemia induced by middle cerebral artery occlusion and 22.5 h of reperfusion infarct size is significantly larger in mutant males compared to wild-type male controls   (MGI Ref ID J:87388)
      • however, no differences in cerebral blood flow in the ischemic hemisphere before, during or after occlusion are detected   (MGI Ref ID J:87388)
  • nervous system phenotype
  • increased cerebral infarction size
    • following 90 min of ischemia induced by middle cerebral artery occlusion and 22.5 h of reperfusion infarct size is significantly larger in mutant males compared to wild-type male controls   (MGI Ref ID J:87388)
    • however, no differences in cerebral blood flow in the ischemic hemisphere before, during or after occlusion are detected   (MGI Ref ID J:87388)
  • behavior/neurological phenotype
  • abnormal spatial learning
    • in a Morris water maze mice show longer escape latencies on each day of testing   (MGI Ref ID J:143076)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Ptger2tm1Brey/Ptger2tm1Brey

        involves: 129S6/SvEvTac * C57BL/6
  • reproductive system phenotype
  • *normal* reproductive system phenotype
    • no defects are detected in oocyte maturation or egg fertilization, in vitro   (MGI Ref ID J:69034)
    • no defects are detected in implantation or decidualization   (MGI Ref ID J:69034)
    • abnormal superovulation
      • at 2 to 8 months of age females produce far fewer eggs relative to wild-type females   (MGI Ref ID J:69034)
      • response to a superovulation protocol is better in young females (3 weeks of age) relative to older females   (MGI Ref ID J:69034)
    • decreased litter size
      • litter sizes from crosses of homozygous males and females were reduced   (MGI Ref ID J:53646)
    • reduced female fertility
      • reduced number of implantations when assessed at E5   (MGI Ref ID J:53646)
      • reduced ovulation and fertilization only about 50% regardless of male genotype   (MGI Ref ID J:53646)
  • cardiovascular system phenotype
  • abnormal vasodilation
    • PGE2-dependent vasodilation effects lost   (MGI Ref ID J:53646)
  • increased systemic arterial blood pressure
    • 8-16 week old females with significantly increased systolic pressure   (MGI Ref ID J:53646)
    • increased systolic pressure in males as well but not statistically significant   (MGI Ref ID J:53646)
    • reversible increase in blood pressure when placed on a high salt diet   (MGI Ref ID J:53646)
  • respiratory system phenotype
  • abnormal airway responsiveness
    • prior treatment with PGE2 fails to reduce the responsiveness to methacholine   (MGI Ref ID J:103373)
  • immune system phenotype
  • abnormal response to infection
    • following infection with Pseudomonas aeruginosa (PA103) bacterial counts in the right lung are lower compared to wild-type controls   (MGI Ref ID J:120725)
  • muscle phenotype
  • abnormal vasodilation
    • PGE2-dependent vasodilation effects lost   (MGI Ref ID J:53646)

Ptger2tm1Brey/Ptger2tm1Brey

        either: (involves: 129S6/SvEvTac) or (involves: 129S6/SvEvTac * C57BL/6)
  • homeostasis/metabolism phenotype
  • abnormal circulating calcium level
    • treatment with PGE2 fails to increase serum calcium levels unlike in wild-type controls   (MGI Ref ID J:77679)

Ptger2tm1Brey/Ptger2tm1Brey

        involves: 129S6/SvEvTac
  • cardiovascular system phenotype
  • abnormal vasodilation
    • norepinephrine precontracted renal afferent arterioles contract rather than dilate in response to PGE2 (1 uM ) or butaprost (1 uM) exposure   (MGI Ref ID J:78902)
    • this constriction response can be reversed by angiotensisn converting enzyme inhibition   (MGI Ref ID J:78902)
  • increased vasoconstriction
    • the renal afferent arteriolar responses to sulprostone and endothelin 1 are increased compared to wild-type controls   (MGI Ref ID J:78902)
  • tumorigenesis
  • decreased incidence of tumors by chemical induction
    • develop fewer lung tumors following a two stage 3-methylcholanthrene and butylated hydroxytoluene protocol   (MGI Ref ID J:116015)
  • decreased tumor growth/size
    • develop smaller lung tumors following a two stage 3-methylcholanthrene and butylated hydroxytoluene protocol   (MGI Ref ID J:116015)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • the decrease in tumor burden is not the result of a decrease in butylated hydroxytoluene induced lung inflammation as this response is similar to wild-type controls   (MGI Ref ID J:116015)
    • decreased incidence of tumors by chemical induction
      • develop fewer lung tumors following a two stage 3-methylcholanthrene and butylated hydroxytoluene protocol   (MGI Ref ID J:116015)
  • muscle phenotype
  • abnormal vasodilation
    • norepinephrine precontracted renal afferent arterioles contract rather than dilate in response to PGE2 (1 uM ) or butaprost (1 uM) exposure   (MGI Ref ID J:78902)
    • this constriction response can be reversed by angiotensisn converting enzyme inhibition   (MGI Ref ID J:78902)
  • increased vasoconstriction
    • the renal afferent arteriolar responses to sulprostone and endothelin 1 are increased compared to wild-type controls   (MGI Ref ID J:78902)

Ptger2tm1Brey/Ptger2tm1Brey

        C.129S6-Ptger2tm1Brey
  • nervous system phenotype
  • abnormal excitatory postsynaptic potential
    • PGE2 and a PTGER2 agonist fail to induce changes in synaptic activity   (MGI Ref ID J:143076)
  • abnormal microglial cell physiology
    • display enhanced phagocytosis of Abeta plaques in an in vitro assay using hippocampal sections from Alzheimer's disease patients   (MGI Ref ID J:97062)
    • microglia show enhanced activation and tend to aggregate around Abeta plaques when incubated with hippocampal sections from Alzheimer disease patients   (MGI Ref ID J:97062)
    • in culture primary microglial cells show some signs of an activated phenotype but do not display an increase in proliferation   (MGI Ref ID J:97062)
    • cultures of mutant microglial cells and wild-type neurons exposed to ABeta1-42 show no signs of the neurotoxicity seen when wild-type microglial cells are used   (MGI Ref ID J:97062)
  • reduced long term potentiation
    • in hippocampal slices at 40 and 120 min after theta burst stimulation   (MGI Ref ID J:143076)
    • LPS fails to elevate LTP at hippocampal perforant path dentate granule cell synapses   (MGI Ref ID J:143076)
  • immune system phenotype
  • abnormal microglial cell physiology
    • display enhanced phagocytosis of Abeta plaques in an in vitro assay using hippocampal sections from Alzheimer's disease patients   (MGI Ref ID J:97062)
    • microglia show enhanced activation and tend to aggregate around Abeta plaques when incubated with hippocampal sections from Alzheimer disease patients   (MGI Ref ID J:97062)
    • in culture primary microglial cells show some signs of an activated phenotype but do not display an increase in proliferation   (MGI Ref ID J:97062)
    • cultures of mutant microglial cells and wild-type neurons exposed to ABeta1-42 show no signs of the neurotoxicity seen when wild-type microglial cells are used   (MGI Ref ID J:97062)
  • hematopoietic system phenotype
  • abnormal microglial cell physiology
    • display enhanced phagocytosis of Abeta plaques in an in vitro assay using hippocampal sections from Alzheimer's disease patients   (MGI Ref ID J:97062)
    • microglia show enhanced activation and tend to aggregate around Abeta plaques when incubated with hippocampal sections from Alzheimer disease patients   (MGI Ref ID J:97062)
    • in culture primary microglial cells show some signs of an activated phenotype but do not display an increase in proliferation   (MGI Ref ID J:97062)
    • cultures of mutant microglial cells and wild-type neurons exposed to ABeta1-42 show no signs of the neurotoxicity seen when wild-type microglial cells are used   (MGI Ref ID J:97062)

Ptger2tm1Brey/Ptger2tm1Brey

        involves: 129S6/SvEvTac * BALB/c
  • immune system phenotype
  • abnormal response to infection
    • LPS induced cerebral oxidative damage is reduced relative to controls   (MGI Ref ID J:164638)
  • nervous system phenotype
  • abnormal nervous system physiology
    • LPS induced cerebral oxidative damage is reduced relative to controls   (MGI Ref ID J:164638)
    • however, kainic acid induced cerebral oxidative damage is not significantly different from wild-type controls   (MGI Ref ID J:164638)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Ptger2tm1Brey related

Cardiovascular Research
Hypertension
      diet-induced

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ptger2tm1Brey
Allele Name targeted mutation 1, Richard M Breyer
Allele Type Targeted (Null/Knockout)
Common Name(s) EP2-;
Mutation Made By Richard Breyer,   Vanderbilt University
Strain of Origin129S6/SvEvTac
ES Cell Line NameTL1/TL-1
ES Cell Line Strain129S6/SvEvTac
Gene Symbol and Name Ptger2, prostaglandin E receptor 2 (subtype EP2)
Chromosome 14
Gene Common Name(s) EP2; EP2 receptor; Ptger-ep2; Ptgerep2; prostaglandin E receptor EP2 subtype;
Molecular Note A PGK-neomycin resistance cassette replaced part of the amino terminal coding sequence, including 420 bp from the ATG start codon to codon 140. [MGI Ref ID J:53646]

Genotyping

Genotyping Information

Genotyping Protocols

Ptger2tm1Brey-Alternate 1, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kennedy CR; Zhang Y; Brandon S; Guan Y; Coffee K; Funk CD; Magnuson MA; Oates JA; Breyer MD; Breyer RM. 1999. Salt-sensitive hypertension and reduced fertility in mice lacking the prostaglandin EP2 receptor. Nat Med 5(2):217-20. [PubMed: 9930871]  [MGI Ref ID J:53646]

Additional References

Ptger2tm1Brey related

Babaev VR; Chew JD; Ding L; Davis S; Breyer MD; Breyer RM; Oates JA; Fazio S; Linton MF. 2008. Macrophage EP4 deficiency increases apoptosis and suppresses early atherosclerosis. Cell Metab 8(6):492-501. [PubMed: 19041765]  [MGI Ref ID J:144376]

Breyer RM; Kennedy CR; Zhang Y; Guan Y; Breyer MD. 2002. Targeted gene disruption of the prostaglandin E2 EP2 receptor. Adv Exp Med Biol 507:321-6. [PubMed: 12664604]  [MGI Ref ID J:87823]

Brouxhon S; Konger RL; VanBuskirk J; Sheu TJ; Ryan J; Erdle B; Almudevar A; Breyer RM; Scott G; Pentland AP. 2007. Deletion of prostaglandin E2 EP2 receptor protects against ultraviolet-induced carcinogenesis, but increases tumor aggressiveness. J Invest Dermatol 127(2):439-46. [PubMed: 16977324]  [MGI Ref ID J:117581]

Brouxhon S; Kyrkanides S; O'Banion MK; Johnson R; Pearce DA; Centola GM; Miller JN; McGrath KH; Erdle B; Scott G; Schneider S; VanBuskirk J; Pentland AP. 2007. Sequential down-regulation of E-cadherin with squamous cell carcinoma progression: loss of E-cadherin via a prostaglandin E2-EP2 dependent posttranslational mechanism. Cancer Res 67(16):7654-64. [PubMed: 17699770]  [MGI Ref ID J:124339]

Chang SH; Ai Y; Breyer RM; Lane TF; Hla T. 2005. The prostaglandin E2 receptor EP2 is required for cyclooxygenase 2-mediated mammary hyperplasia. Cancer Res 65(11):4496-9. [PubMed: 15930264]  [MGI Ref ID J:98801]

Chen J; Zhao M; He W; Milne GL; Howard JR; Morrow J; Hebert RL; Breyer RM; Chen J; Hao CM. 2008. Increased dietary NaCl induces renal medullary PGE2 production and natriuresis via the EP2 receptor. Am J Physiol Renal Physiol 295(3):F818-25. [PubMed: 18632796]  [MGI Ref ID J:148637]

Choudhary S; Blackwell K; Voznesensky O; Deb Roy A; Pilbeam C. 2013. Prostaglandin E2 acts via bone marrow macrophages to block PTH-stimulated osteoblast differentiation in vitro. Bone 56(1):31-41. [PubMed: 23639875]  [MGI Ref ID J:203565]

Chun KS; Lao HC; Trempus CS; Okada M; Langenbach R. 2009. The prostaglandin receptor EP2 activates multiple signaling pathways and beta-arrestin1 complex formation during mouse skin papilloma development. Carcinogenesis 30(9):1620-7. [PubMed: 19587094]  [MGI Ref ID J:152191]

Cimino PJ; Sokal I; Leverenz J; Fukui Y; Montine TJ. 2009. DOCK2 is a microglial specific regulator of central nervous system innate immunity found in normal and Alzheimer's disease brain. Am J Pathol 175(4):1622-30. [PubMed: 19729484]  [MGI Ref ID J:153055]

Fortner CN; Breyer RM; Paul RJ. 2001. EP2 receptors mediate airway relaxation to substance P, ATP, and PGE2. Am J Physiol Lung Cell Mol Physiol 281(2):L469-74. [PubMed: 11435222]  [MGI Ref ID J:108642]

Hoggatt J; Mohammad KS; Singh P; Hoggatt AF; Chitteti BR; Speth JM; Hu P; Poteat BA; Stilger KN; Ferraro F; Silberstein L; Wong FK; Farag SS; Czader M; Milne GL; Breyer RM; Serezani CH; Scadden DT; Guise TA; Srour EF; Pelus LM. 2013. Differential stem- and progenitor-cell trafficking by prostaglandin E2. Nature 495(7441):365-9. [PubMed: 23485965]  [MGI Ref ID J:195129]

Imig JD; Breyer MD; Breyer RM. 2002. Contribution of prostaglandin EP(2) receptors to renal microvascular reactivity in mice. Am J Physiol Renal Physiol 283(3):F415-22. [PubMed: 12167591]  [MGI Ref ID J:78902]

Johansson JU; Pradhan S; Lokteva LA; Woodling NS; Ko N; Brown HD; Wang Q; Loh C; Cekanaviciute E; Buckwalter M; Manning-Bog AB; Andreasson KI. 2013. Suppression of inflammation with conditional deletion of the prostaglandin E2 EP2 receptor in macrophages and brain microglia. J Neurosci 33(40):16016-32. [PubMed: 24089506]  [MGI Ref ID J:202677]

Keene CD; Chang R; Stephen C; Nivison M; Nutt SE; Look A; Breyer RM; Horner PJ; Hevner R; Montine TJ. 2009. Protection of hippocampal neurogenesis from toll-like receptor 4-dependent innate immune activation by ablation of prostaglandin E2 receptor subtype EP1 or EP2. Am J Pathol 174(6):2300-9. [PubMed: 19389932]  [MGI Ref ID J:148777]

Keith RL; Geraci MW; Nana-Sinkam SP; Breyer RM; Hudish TM; Meyer AM; Malkinson AM; Dwyer-Nield LD. 2006. Prostaglandin E2 receptor subtype 2 (EP2) null mice are protected against murine lung tumorigenesis. Anticancer Res 26(4B):2857-61. [PubMed: 16886605]  [MGI Ref ID J:116015]

Li X; Tomita M; Pilbeam CC; Breyer RM; Raisz LG. 2002. Prostaglandin receptor EP2 mediates PGE2 stimulated hypercalcemia in mice in vivo. Prostaglandins Other Lipid Mediat 67(3-4):173-80. [PubMed: 12013525]  [MGI Ref ID J:77679]

Liang X; Wang Q; Hand T; Wu L; Breyer RM; Montine TJ; Andreasson K. 2005. Deletion of the prostaglandin E2 EP2 receptor reduces oxidative damage and amyloid burden in a model of Alzheimer's disease. J Neurosci 25(44):10180-7. [PubMed: 16267225]  [MGI Ref ID J:102728]

Mason KL; Rogers LM; Soares EM; Bani-Hashemi T; Erb Downward J; Agnew D; Peters-Golden M; Weinberg JB; Crofford LJ; Aronoff DM. 2013. Intrauterine group A streptococcal infections are exacerbated by prostaglandin E2. J Immunol 191(5):2457-65. [PubMed: 23913961]  [MGI Ref ID J:205810]

Matsumoto H; Ma W; Smalley W; Trzaskos J; Breyer RM; Dey SK. 2001. Diversification of cyclooxygenase-2-derived prostaglandins in ovulation and implantation. Biol Reprod 64(5):1557-65. [PubMed: 11319164]  [MGI Ref ID J:69034]

McCullough L; Wu L; Haughey N; Liang X; Hand T; Wang Q; Breyer RM; Andreasson K. 2004. Neuroprotective function of the PGE2 EP2 receptor in cerebral ischemia. J Neurosci 24(1):257-68. [PubMed: 14715958]  [MGI Ref ID J:87388]

Montine TJ; Milatovic D; Gupta RC; Valyi-Nagy T; Morrow JD; Breyer RM. 2002. Neuronal oxidative damage from activated innate immunity is EP2 receptor-dependent. J Neurochem 83(2):463-70. [PubMed: 12423256]  [MGI Ref ID J:164638]

Ochsner SA; Russell DL; Day AJ; Breyer RM; Richards JS. 2003. Decreased expression of tumor necrosis factor-alpha-stimulated gene 6 in cumulus cells of the cyclooxygenase-2 and EP2 null mice. Endocrinology 144(3):1008-19. [PubMed: 12586778]  [MGI Ref ID J:115517]

Ono K; Kaneko H; Choudhary S; Pilbeam CC; Lorenzo JA; Akatsu T; Kugai N; Raisz LG. 2005. Biphasic effect of prostaglandin E2 on osteoclast formation in spleen cell cultures: role of the EP2 receptor. J Bone Miner Res 20(1):23-9. [PubMed: 15619666]  [MGI Ref ID J:101564]

Sadikot RT; Zeng H; Azim AC; Joo M; Dey SK; Breyer RM; Peebles RS; Blackwell TS; Christman JW. 2007. Bacterial clearance of Pseudomonas aeruginosa is enhanced by the inhibition of COX-2. Eur J Immunol 37(4):1001-9. [PubMed: 17330822]  [MGI Ref ID J:120725]

Sheller JR; Mitchell D; Meyrick B; Oates J; Breyer R. 2000. EP(2) receptor mediates bronchodilation by PGE(2) in mice. J Appl Physiol 88(6):2214-8. [PubMed: 10846038]  [MGI Ref ID J:103373]

Shie FS; Breyer RM; Montine TJ. 2005. Microglia Lacking E Prostanoid Receptor Subtype 2 Have Enhanced A{beta} Phagocytosis yet Lack A{beta}-Activated Neurotoxicity. Am J Pathol 166(4):1163-72. [PubMed: 15793296]  [MGI Ref ID J:97062]

Shie FS; Montine KS; Breyer RM; Montine TJ. 2005. Microglial EP2 is critical to neurotoxicity from activated cerebral innate immunity. Glia 52(1):70-7. [PubMed: 15920732]  [MGI Ref ID J:156154]

Sinha P; Clements VK; Fulton AM; Ostrand-Rosenberg S. 2007. Prostaglandin E2 promotes tumor progression by inducing myeloid-derived suppressor cells. Cancer Res 67(9):4507-13. [PubMed: 17483367]  [MGI Ref ID J:121303]

Tsutsumi R; Xie C; Wei X; Zhang M; Zhang X; Flick LM; Schwarz EM; O'Keefe RJ. 2009. PGE2 signaling through the EP4 receptor on fibroblasts upregulates RANKL and stimulates osteolysis. J Bone Miner Res 24(10):1753-62. [PubMed: 19419302]  [MGI Ref ID J:160218]

Yang H; Zhang J; Breyer RM; Chen C. 2009. Altered hippocampal long-term synaptic plasticity in mice deficient in the PGE2 EP2 receptor. J Neurochem 108(1):295-304. [PubMed: 19012750]  [MGI Ref ID J:143076]

Zahner G; Schaper M; Panzer U; Kluger M; Stahl RA; Thaiss F; Schneider A. 2009. Prostaglandin EP2 and EP4 receptors modulate expression of the chemokine CCL2 (MCP-1) in response to LPS-induced renal glomerular inflammation. Biochem J 422(3):563-70. [PubMed: 19570035]  [MGI Ref ID J:155717]

Zaslona Z; Serezani CH; Okunishi K; Aronoff DM; Peters-Golden M. 2012. Prostaglandin E2 restrains macrophage maturation via E prostanoid receptor 2/protein kinase A signaling. Blood 119(10):2358-67. [PubMed: 22234697]  [MGI Ref ID J:182557]

Zhang Y; Guan Y; Schneider A; Brandon S; Breyer RM; Breyer MD. 2000. Characterization of murine vasopressor and vasodepressor prostaglandin E(2) receptors Hypertension 35(5):1129-34. [PubMed: 10818076]  [MGI Ref ID J:62425]

Zhu S; Xue R; Zhao P; Fan FL; Kong X; Zheng S; Han Q; Zhu Y; Wang N; Yang J; Guan Y. 2011. Targeted disruption of the prostaglandin E2 E-prostanoid 2 receptor exacerbates vascular neointimal formation in mice. Arterioscler Thromb Vasc Biol 31(8):1739-47. [PubMed: 21636806]  [MGI Ref ID J:191856]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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