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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names S3 (Changed: 15-DEC-04 ) TgN(Cga-cre)S3Sac (Changed: 15-DEC-04 ) alpha GSU-cre (Changed: 15-DEC-04 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation p+N2pN1p (09-NOV-05) Donating Investigator Sally Camper, University of Michigan Medical School Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express the Cre recombinase under the control of the mouse glycoprotein hormone alpha-subunit promoter. Cre recombinase expression is detected in the anterior and intermediate lobes of the pituitary gland, as well as in cardiac and skeletal muscle. Low to no level of expression is detected in the posterior pituitary, lungs, kidneys, brain, adrenal glands and gonads. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the anterior pituitary. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study developmentally critical gene function in the anterior pituitary.Development
A transgenic construct containing a 4.6Kb sequence of mouse glycoprotein hormone alpha-subunit promoter upstream of the Cre recombinase gene, a nuclear localization signal, and beta-actin polyadenylation site sequence was injected into B6SJLF2 zygotes. Transgenic founder mice were obtained.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Cga
002494 B6;129S2-Cgatm1Sac/J View Strains carrying other alleles of Cga (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Cga-cre)3Sac | ||
|---|---|---|---|
| Allele Name | transgene insertion 3, Sally A Camper | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | S3; TgN(Cga-cre)S3Sac; alphaGSU-cre; | ||
| Mutation Made By | Sally Camper, University of Michigan Medical School | ||
| Strain of Origin | (C57BL/6J x SJL)F2 | ||
| Site of Expression | anterior and intermediate lobes of the pituitary gland, as well as in cardiac and skeletal muscle; low to no level of expression is detected in the posterior pituitary, lungs, kidneys, brain, adrenal gland and gonads | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Cga, glycoprotein hormones, alpha subunit, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Cga-cre)3Sac, transgene insertion 3, Sally A Camper | ||
| Chromosome | UN | ||
| Gene Common Name(s) | S3; TgN(Cga-cre)S3Sac; alphaGSU-cre; | ||
| Driver Note | Cga | ||
| General Note | Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a mouse glycoprotein hormone alpha-subunit (Cga) gene. This promoter is active in the pituitary primordium beginning at embryonic day 9.5. Cre recombinase expression is detected in the anterior and intermediate lobes of the pituitary gland, in cardiac, and in skeletal muscle. Low to no level of expression is detected in the posterior pituitary, lungs, kidneys, brain, adrenal glands, and gonads. [MGI Ref ID J:66897] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre Quantitative PCR, QPCR
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Cushman LJ; Burrows HL; Seasholtz AF; Lewandoski M; Muzyczka N; Camper SA. 2000. Cre-mediated recombination in the pituitary gland Genesis 28(3-4):167-74. [PubMed: 11105060] [MGI Ref ID J:66897]
Additional References
Zhao L; Bakke M; Krimkevich Y; Cushman LJ; Parlow AF; Camper SA; Parker KL. 2001. Hypomorphic phenotype in mice with pituitary-specific knockout of steroidogenic factor 1. Genesis 30(2):65-9. [PubMed: 11416865] [MGI Ref ID J:70412]
Zhao L; Bakke M; Krimkevich Y; Cushman LJ; Parlow AF; Camper SA; Parker KL. 2001. Steroidogenic factor 1 (SF1) is essential for pituitary gonadotrope function. Development 128(2):147-54. [PubMed: 11124111] [MGI Ref ID J:66593]
Tg(Cga-cre)3Sac relatedBakke M; Zhao L; Parker KL. 2001. Approaches to define the role of SF-1 at different levels of the hypothalamic-pituitary-steroidogenic organ axis. Mol Cell Endocrinol 179(1-2):33-7. [PubMed: 11420128] [MGI Ref ID J:125681]
Bliss SP; Miller A; Navratil AM; Xie J; McDonough SP; Fisher PJ; Landreth GE; Roberson MS. 2009. ERK signaling in the pituitary is required for female but not male fertility. Mol Endocrinol 23(7):1092-101. [PubMed: 19372235] [MGI Ref ID J:150031]
Charles MA; Saunders TL; Wood WM; Owens K; Parlow AF; Camper SA; Ridgway EC; Gordon DF. 2006. Pituitary-specific gata2 knockout: effects on gonadotrope and thyrotrope function. Mol Endocrinol 20(6):1366-77. [PubMed: 16543408] [MGI Ref ID J:108963]
Davis SW; Camper SA. 2007. Noggin regulates Bmp4 activity during pituitary induction. Dev Biol 305(1):145-60. [PubMed: 17359964] [MGI Ref ID J:121318]
Gieske MC; Kim HJ; Legan SJ; Koo Y; Krust A; Chambon P; Ko C. 2008. Pituitary gonadotroph estrogen receptor-alpha is necessary for fertility in females. Endocrinology 149(1):20-7. [PubMed: 17947360] [MGI Ref ID J:131949]
Nasonkin IO; Potok MA; Camper SA. 2009. Cre-mediated recombination in pituitary somatotropes. Genesis 47(1):55-60. [PubMed: 19039787] [MGI Ref ID J:144723]
Zhao L; Bakke M; Krimkevich Y; Cushman LJ; Parlow AF; Camper SA; Parker KL. 2001. Hypomorphic phenotype in mice with pituitary-specific knockout of steroidogenic factor 1. Genesis 30(2):65-9. [PubMed: 11416865] [MGI Ref ID J:70412]
Zhao L; Bakke M; Krimkevich Y; Cushman LJ; Parlow AF; Camper SA; Parker KL. 2001. Steroidogenic factor 1 (SF1) is essential for pituitary gonadotrope function. Development 128(2):147-54. [PubMed: 11124111] [MGI Ref ID J:66593]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry Transgenic animals originated on a B6SJLF2 background. When held in a live colony, the strain is maintained as a hemizygote. Coat color expected from breeding:Black Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 Cryopreserved Embryos $1600.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 Cryopreserved Embryos $2080.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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