Description

Strain Information

Former Names B6.129S4-Scya2tm1Rol    (Changed: 15-DEC-04 ) MCP-1 -/-    (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Generation N10F?+9 (20-DEC-06)   Donating Investigator Barrett Rollins,   Dana-Farber Cancer Inst, Harvard Med Sch

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product is detected in lipopolysaccharide (LPS) -stimulated peritoneal macrophages isolated from homozygous mice. The numbers of peritoneal macrophages, Kupffer cell and alveolar macrophages were similar to levels found in wildtype mice. Thioglycollate induced peritonitis results in impaired recruitment of monocytes and macrophages to peritoneal cavity. Cellular recruitment to delayed-type hypersensitivity challenges and secondary granulomata is reduced. This mutant mouse strain represents a model that may be useful in studies related to leukocyte trafficking.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 2 and insert an inframe stop codon into exon 1. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Ccl2^tm1Rol/Ccl2^tm1Rol

        involves: 129S4/SvJae * 129X1/SvJ * C57BL/6

• immune system phenotype
• abnormal interleukin secretion (MGI Ref ID J:45920)
  • reduced IL-4 and IL-5 production in splenocytes
• decreased interferon-gamma secretion (MGI Ref ID J:45920)
  • reduced Ifn-gamma production in splenocytes
• impaired macrophage recruitment (MGI Ref ID J:45920)
  • impaired macrophage recruitment following intraperitoneal thioglycollate injection

Ccl2^tm1Rol/Ccl2^tm1Rol

        involves: 129S4/SvJae * C57BL/6

• cardiovascular system phenotype
• abnormal physiological neovascularization (MGI Ref ID J:107646)
  • ischemic capillary density relative to nonischemic density 30% lower than in controls
  • poor recovery of blood flow after ischemia

Ccl2^tm1Rol/Ccl2^tm1Rol

        involves: 129S4/SvJae

• immune system phenotype
• abnormal monocyte morphology (MGI Ref ID J:121281)
  • 7/4^bri and 7/4^dim cells are decreased in blood by 50%
  • intraperitoneal thioglycollate challenge leads to an increase in 7/4^bri cells
• hematopoietic system phenotype
• abnormal monocyte morphology (MGI Ref ID J:121281)
  • 7/4^bri and 7/4^dim cells are decreased in blood by 50%
  • intraperitoneal thioglycollate challenge leads to an increase in 7/4^bri cells

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Ccl2^tm1Rol related

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology and Inflammation Research
Inflammation

Internal/Organ Research
Wound Healing (delayed/impaired)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Ccl2tm1Rol
Allele Name		targeted mutation 1, Barrett J Rollins
Allele Type		Targeted (knock-out)
Common Name(s)		(MCP)1-; CCL2-; CCL2KO; MCP-1-; MCP-1KO; Scya2-;
Mutation Made By		Barrett Rollins,   Dana-Farber Cancer Inst, Harvard Med Sch
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Ccl2, chemokine (C-C motif) ligand 2
Chromosome		11
Gene Common Name(s)		AI323594; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; MGC9434; SCYA2; SMC-CF; Scya2; Sigje; expressed sequence AI323594; monocyte chemoattractant protein-1; monocyte chemotactic protein; small inducible cytokine A2; small inducible gene JE;
General Note		Phenotypic Similarity to Human Syndrome: Age-related macular degeneration (for Ccl2tm1Rol Cx3cr1tm1Zm homozygotes J:126935)
Molecular Note		Insertion of a PGK-neomycin resistance cassette into exon 2 introduced a small deletion and an inframe stop codon into exon 1. Secreted protein was not detected in stimulated peritoneal macrophages from homozygous mutant mice. [MGI Ref ID J:45920]

Genotyping

Genotyping Information

Genotyping Protocols

Ccl2^tm1Rol, STD PCR, vers. 2

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Lu B; Rutledge BJ; Gu L; Fiorillo J; Lukacs NW; Kunkel SL; North R; Gerard C; Rollins BJ. 1998. Abnormalities in monocyte recruitment and cytokine expression in monocyte chemoattractant protein 1-deficient mice. J Exp Med 187(4):601-8. [PubMed: 9463410]  [MGI Ref ID J:45920]

Additional References

Gonnella PA; Kodali D; Weiner HL. 2003. Induction of low dose oral tolerance in monocyte chemoattractant protein-1- and CCR2-deficient mice. J Immunol 170(5):2316-22. [PubMed: 12594253]  [MGI Ref ID J:82016]

Gu L; Okada Y; Clinton SK; Gerard C; Sukhova GK; Libby P; Rollins BJ. 1998. Absence of monocyte chemoattractant protein-1 reduces atherosclerosis in low density lipoprotein receptor-deficient mice. Mol Cell 2(2):275-81. [PubMed: 9734366]  [MGI Ref ID J:75837]

Huang D; Wang J; Kivisakk P; Rollins BJ; Ransohoff RM. 2001. Absence of monocyte chemoattractant protein 1 in mice leads to decreased local macrophage recruitment and antigen-specific t helper cell type 1 immune response in experimental autoimmune encephalomyelitis. J Exp Med 193(6):713-26. [PubMed: 11257138]  [MGI Ref ID J:68145]

Kawakami K; Kinjo Y; Uezu K; Yara S; Miyagi K; Koguchi Y; Nakayama T; Taniguchi M; Saito A. 2001. Monocyte chemoattractant protein-1-dependent increase of Valpha14 NKT cells in lungs and their roles in Th1 response and host defense in cryptococcal infection. J Immunol 167(11):6525-32. [PubMed: 11714821]  [MGI Ref ID J:72823]

Low QE; Drugea IA; Duffner LA; Quinn DG; Cook DN; Rollins BJ; Kovacs EJ; DiPietro LA. 2001. Wound healing in MIP-1alpha(-/-) and MCP-1(-/-) mice. Am J Pathol 159(2):457-63. [PubMed: 11485904]  [MGI Ref ID J:70862]

Muessel MJ; Klein RM; Wilson AM; Berman NE. 2002. Ablation of the chemokine monocyte chemoattractant protein-1 delays retrograde neuronal degeneration, attenuates microglial activation, and alters expression of cell death molecules. Brain Res Mol Brain Res 103(1-2):12-27. [PubMed: 12106688]  [MGI Ref ID J:78320]

Ccl2^tm1Rol related

Abonia JP; Austen KF; Rollins BJ; Joshi SK; Flavell RA; Kuziel WA; Koni PA; Gurish MF. 2005. Constitutive homing of mast cell progenitors to the intestine depends on autologous expression of the chemokine receptor CXCR2. Blood 105(11):4308-13. [PubMed: 15705791]  [MGI Ref ID J:98967]

Bai Y; Liu R; Huang D; La Cava A; Tang YY; Iwakura Y; Campagnolo DI; Vollmer TL; Ransohoff RM; Shi FD. 2008. CCL2 recruitment of IL-6-producing CD11b(+) monocytes to the draining lymph nodes during the initiation of Th17-dependent B cell-mediated autoimmunity. Eur J Immunol 38(7):1877-88. [PubMed: 18581322]  [MGI Ref ID J:137386]

Bandukwala HS; Clay BS; Tong J; Mody PD; Cannon JL; Shilling RA; Verbeek JS; Weinstock JV; Solway J; Sperling AI. 2007. Signaling through Fc gamma RIII is required for optimal T helper type (Th)2 responses and Th2-mediated airway inflammation. J Exp Med 204(8):1875-89. [PubMed: 17664287]  [MGI Ref ID J:125951]

Barlic J; Murphy PM. 2007. Chemokine regulation of atherosclerosis. J Leukoc Biol 82(2):226-36. [PubMed: 17329566]  [MGI Ref ID J:123530]

Bitsaktsis C; Winslow G. 2006. Fatal recall responses mediated by CD8 T cells during intracellular bacterial challenge infection. J Immunol 177(7):4644-51. [PubMed: 16982903]  [MGI Ref ID J:139316]

Blednov YA; Bergeson SE; Walker D; Ferreira VM; Kuziel WA; Harris RA. 2005. Perturbation of chemokine networks by gene deletion alters the reinforcing actions of ethanol. Behav Brain Res 165(1):110-25. [PubMed: 16105698]  [MGI Ref ID J:102583]

Capoccia BJ; Gregory AD; Link DC. 2008. Recruitment of the inflammatory subset of monocytes to sites of ischemia induces angiogenesis in a monocyte chemoattractant protein-1-dependent fashion. J Leukoc Biol 84(3):760-8. [PubMed: 18550788]  [MGI Ref ID J:138153]

Chow FY; Nikolic-Paterson DJ; Ozols E; Atkins RC; Rollin BJ; Tesch GH. 2006. Monocyte chemoattractant protein-1 promotes the development of diabetic renal injury in streptozotocin-treated mice. Kidney Int 69(1):73-80. [PubMed: 16374426]  [MGI Ref ID J:136515]

Conrad SM; Strauss-Ayali D; Field AE; Mack M; Mosser DM. 2007. Leishmania-derived murine monocyte chemoattractant protein 1 enhances the recruitment of a restrictive population of CC chemokine receptor 2-positive macrophages. Infect Immun 75(2):653-65. [PubMed: 17088347]  [MGI Ref ID J:117724]

D'Avila H; Melo RC; Parreira GG; Werneck-Barroso E; Castro-Faria-Neto HC; Bozza PT. 2006. Mycobacterium bovis bacillus Calmette-Guerin induces TLR2-mediated formation of lipid bodies: intracellular domains for eicosanoid synthesis in vivo. J Immunol 176(5):3087-97. [PubMed: 16493068]  [MGI Ref ID J:129415]

DePaolo RW; Rollins BJ; Kuziel W; Karpus WJ. 2003. CC chemokine ligand 2 and its receptor regulate mucosal production of IL-12 and TGF-beta in high dose oral tolerance. J Immunol 171(7):3560-7. [PubMed: 14500652]  [MGI Ref ID J:85628]

DeSchoolmeester ML; Little MC; Rollins BJ; Else KJ. 2003. Absence of CC Chemokine Ligand 2 Results in an Altered Th1/Th2 Cytokine Balance and Failure to Expel Trichuris muris Infection. J Immunol 170(9):4693-700. [PubMed: 12707348]  [MGI Ref ID J:82997]

Dessing MC; de Vos AF; Florquin S; van der Poll T. 2006. Monocyte chemoattractant protein 1 does not contribute to protective immunity against pneumococcal pneumonia. Infect Immun 74(12):7021-3. [PubMed: 16982835]  [MGI Ref ID J:115915]

Dessing MC; van der Sluijs KF; Florquin S; van der Poll T. 2007. Monocyte chemoattractant protein 1 contributes to an adequate immune response in influenza pneumonia. Clin Immunol 125(3):328-36. [PubMed: 17827068]  [MGI Ref ID J:128006]

Dewald O; Zymek P; Winkelmann K; Koerting A; Ren G; Abou-Khamis T; Michael LH; Rollins BJ; Entman ML; Frangogiannis NG. 2005. CCL2/Monocyte Chemoattractant Protein-1 regulates inflammatory responses critical to healing myocardial infarcts. Circ Res 96(8):881-9. [PubMed: 15774854]  [MGI Ref ID J:108533]

Dogan RN; Elhofy A; Karpus WJ. 2008. Production of CCL2 by central nervous system cells regulates development of murine experimental autoimmune encephalomyelitis through the recruitment of TNF- and iNOS-expressing macrophages and myeloid dendritic cells. J Immunol 180(11):7376-84. [PubMed: 18490737]  [MGI Ref ID J:136335]

Dunay IR; Damatta RA; Fux B; Presti R; Greco S; Colonna M; Sibley LD. 2008. Gr1(+) Inflammatory Monocytes Are Required for Mucosal Resistance to the Pathogen Toxoplasma gondii. Immunity 29(2):306-17. [PubMed: 18691912]  [MGI Ref ID J:139568]

Felton LM; Cunningham C; Rankine EL; Waters S; Boche D; Perry VH. 2005. MCP-1 and murine prion disease: separation of early behavioural dysfunction from overt clinical disease. Neurobiol Dis 20(2):283-95. [PubMed: 15886005]  [MGI Ref ID J:102784]

Ferreira AM; Takagawa S; Fresco R; Zhu X; Varga J; DiPietro LA. 2006. Diminished induction of skin fibrosis in mice with MCP-1 deficiency. J Invest Dermatol 126(8):1900-8. [PubMed: 16691201]  [MGI Ref ID J:110695]

Fischer S; Kleinschnitz C; Muller M; Kobsar I; Ip CW; Rollins B; Martini R. 2008. Monocyte chemoattractant protein-1 is a pathogenic component in a model for a hereditary peripheral neuropathy. Mol Cell Neurosci 37(2):359-66. [PubMed: 18326085]  [MGI Ref ID J:133028]

Frangogiannis NG; Dewald O; Xia Y; Ren G; Haudek S; Leucker T; Kraemer D; Taffet G; Rollins BJ; Entman ML. 2007. Critical role of monocyte chemoattractant protein-1/CC chemokine ligand 2 in the pathogenesis of ischemic cardiomyopathy. Circulation 115(5):584-92. [PubMed: 17283277]  [MGI Ref ID J:130611]

Ge S; Song L; Serwanski DR; Kuziel WA; Pachter JS. 2008. Transcellular transport of CCL2 across brain microvascular endothelial cells. J Neurochem 104(5):1219-32. [PubMed: 18289346]  [MGI Ref ID J:131712]

Gonnella PA; Kodali D; Weiner HL. 2003. Induction of low dose oral tolerance in monocyte chemoattractant protein-1- and CCR2-deficient mice. J Immunol 170(5):2316-22. [PubMed: 12594253]  [MGI Ref ID J:82016]

Graves DT; Alsulaimani F; Ding Y; Marks SC Jr. 2002. Developmentally regulated monocyte recruitment and bone resorption are modulated by functional deletion of the monocytic chemoattractant protein-1 gene. Bone 31(2):282-7. [PubMed: 12151080]  [MGI Ref ID J:109290]

Gregory JL; Morand EF; McKeown SJ; Ralph JA; Hall P; Yang YH; McColl SR; Hickey MJ. 2006. Macrophage migration inhibitory factor induces macrophage recruitment via CC chemokine ligand 2. J Immunol 177(11):8072-9. [PubMed: 17114481]  [MGI Ref ID J:140686]

Gu L; Okada Y; Clinton SK; Gerard C; Sukhova GK; Libby P; Rollins BJ. 1998. Absence of monocyte chemoattractant protein-1 reduces atherosclerosis in low density lipoprotein receptor-deficient mice. Mol Cell 2(2):275-81. [PubMed: 9734366]  [MGI Ref ID J:75837]

Gu L; Tseng S; Horner RM; Tam C; Loda M; Rollins BJ. 2000. Control of TH2 polarization by the chemokine monocyte chemoattractant protein-1. Nature 404(6776):407-11. [PubMed: 10746730]  [MGI Ref ID J:61420]

Held KS; Chen BP; Kuziel WA; Rollins BJ; Lane TE. 2004. Differential roles of CCL2 and CCR2 in host defense to coronavirus infection. Virology 329(2):251-60. [PubMed: 15518805]  [MGI Ref ID J:102446]

Henderson RB; Hobbs JA; Mathies M; Hogg N. 2003. Rapid recruitment of inflammatory monocytes is independent of neutrophil migration. Blood 102(1):328-35. [PubMed: 12623845]  [MGI Ref ID J:115516]

Hokeness KL; Kuziel WA; Biron CA; Salazar-Mather TP. 2005. Monocyte chemoattractant protein-1 and CCR2 interactions are required for IFN-alpha/beta-induced inflammatory responses and antiviral defense in liver. J Immunol 174(3):1549-56. [PubMed: 15661915]  [MGI Ref ID J:96407]

Huang D; Wang J; Kivisakk P; Rollins BJ; Ransohoff RM. 2001. Absence of monocyte chemoattractant protein 1 in mice leads to decreased local macrophage recruitment and antigen-specific t helper cell type 1 immune response in experimental autoimmune encephalomyelitis. J Exp Med 193(6):713-26. [PubMed: 11257138]  [MGI Ref ID J:68145]

Inouye KE; Shi H; Howard JK; Daly CH; Lord GM; Rollins BJ; Flier JS. 2007. Absence of CC chemokine ligand 2 does not limit obesity-associated infiltration of macrophages into adipose tissue. Diabetes 56(9):2242-50. [PubMed: 17473219]  [MGI Ref ID J:126512]

Jia T; Serbina NV; Brandl K; Zhong MX; Leiner IM; Charo IF; Pamer EG. 2008. Additive roles for MCP-1 and MCP-3 in CCR2-mediated recruitment of inflammatory monocytes during Listeria monocytogenes infection. J Immunol 180(10):6846-53. [PubMed: 18453605]  [MGI Ref ID J:134953]

Kalkonde YV; Morgan WW; Sigala J; Maffi SK; Condello C; Kuziel W; Ahuja SS; Ahuja SK. 2007. Chemokines in the MPTP model of Parkinson's disease: Absence of CCL2 and its receptor CCR2 does not protect against striatal neurodegeneration. Brain Res 1128(1):1-11. [PubMed: 17126305]  [MGI Ref ID J:117686]

Kanda H; Tateya S; Tamori Y; Kotani K; Hiasa K; Kitazawa R; Kitazawa S; Miyachi H; Maeda S; Egashira K; Kasuga M. 2006. MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity. J Clin Invest 116(6):1494-505. [PubMed: 16691291]  [MGI Ref ID J:110277]

Kawakami K; Kinjo Y; Uezu K; Yara S; Miyagi K; Koguchi Y; Nakayama T; Taniguchi M; Saito A. 2001. Monocyte chemoattractant protein-1-dependent increase of Valpha14 NKT cells in lungs and their roles in Th1 response and host defense in cryptococcal infection. J Immunol 167(11):6525-32. [PubMed: 11714821]  [MGI Ref ID J:72823]

Kawakami K; Yamamoto N; Kinjo Y; Miyagi K; Nakasone C; Uezu K; Kinjo T; Nakayama T; Taniguchi M; Saito A. 2003. Critical role of Valpha14+ natural killer T cells in the innate phase of host protection against Streptococcus pneumoniae infection. Eur J Immunol 33(12):3322-30. [PubMed: 14635040]  [MGI Ref ID J:87141]

Khan WI; Motomura Y; Wang H; El-Sharkawy RT; Verdu EF; Verma-Gandhu M; Rollins BJ; Collins SM. 2006. Critical role of MCP-1 in the pathogenesis of experimental colitis in the context of immune and enterochromaffin cells. Am J Physiol Gastrointest Liver Physiol 291(5):G803-11. [PubMed: 16728728]  [MGI Ref ID J:116878]

Kim B; Sarangi PP; Lee Y; Deshpande Kaistha S; Lee S; Rouse BT. 2006. Depletion of MCP-1 increases development of herpetic stromal keratitis by innate immune modulation. J Leukoc Biol 80(6):1405-15. [PubMed: 16997857]  [MGI Ref ID J:116570]

Kirk EA; Sagawa ZK; McDonald TO; O'Brien KD; Heinecke JW. 2008. Macrophage chemoattractant protein-1 deficiency fails to restrain macrophage infiltration into adipose tissue. Diabetes 57(5):1254-61. [PubMed: 18268047]  [MGI Ref ID J:135249]

Lee I; Wang L; Wells AD; Ye Q; Han R; Dorf ME; Kuziel WA; Rollins BJ; Chen L; Hancock WW. 2003. Blocking the monocyte chemoattractant protein-1/CCR2 chemokine pathway induces permanent survival of islet allografts through a programmed death-1 ligand-1-dependent mechanism. J Immunol 171(12):6929-35. [PubMed: 14662900]  [MGI Ref ID J:118487]

Low QE; Drugea IA; Duffner LA; Quinn DG; Cook DN; Rollins BJ; Kovacs EJ; DiPietro LA. 2001. Wound healing in MIP-1alpha(-/-) and MCP-1(-/-) mice. Am J Pathol 159(2):457-63. [PubMed: 11485904]  [MGI Ref ID J:70862]

Mignot G; Ullrich E; Bonmort M; Menard C; Apetoh L; Taieb J; Bosisio D; Sozzani S; Ferrantini M; Schmitz J; Mack M; Ryffel B; Bulfone-Paus S; Zitvogel L; Chaput N. 2008. The critical role of IL-15 in the antitumor effects mediated by the combination therapy imatinib and IL-2. J Immunol 180(10):6477-83. [PubMed: 18453565]  [MGI Ref ID J:134964]

Moore BB; Murray L; Das A; Wilke CA; Herrygers AB; Toews GB. 2006. The role of CCL12 in the recruitment of fibrocytes and lung fibrosis. Am J Respir Cell Mol Biol 35(2):175-81. [PubMed: 16543609]  [MGI Ref ID J:123659]

Moore RJ; Owens DM; Stamp G; Arnott C; Burke F; East N; Holdsworth H; Turner L; Rollins B; Pasparakis M; Kollias G; Balkwill F. 1999. Mice deficient in tumor necrosis factor-alpha are resistant to skin carcinogenesis. Nat Med 5(7):828-831. [PubMed: 10395330]  [MGI Ref ID J:56068]

Nakao S; Kuwano T; Tsutsumi-Miyahara C; Ueda SI; Kimura YN; Hamano S; Sonoda KH; Saijo Y; Nukiwa T; Strieter RM; Ishibashi T; Kuwano M; Ono M. 2005. Infiltration of COX-2-expressing macrophages is a prerequisite for IL-1beta-induced neovascularization and tumor growth. J Clin Invest 115(11):2979-2991. [PubMed: 16239969]  [MGI Ref ID J:102498]

Nakazawa T; Hisatomi T; Nakazawa C; Noda K; Maruyama K; She H; Matsubara A; Miyahara S; Nakao S; Yin Y; Benowitz L; Hafezi-Moghadam A; Miller JW. 2007. Monocyte chemoattractant protein 1 mediates retinal detachment-induced photoreceptor apoptosis. Proc Natl Acad Sci U S A 104(7):2425-30. [PubMed: 17284607]  [MGI Ref ID J:119752]

O'Shea M; Maytham EG; Linehan JM; Brandner S; Collinge J; Lloyd SE. 2008. Investigation of mcp1 as a quantitative trait gene for prion disease incubation time in mouse. Genetics 180(1):559-66. [PubMed: 18716327]  [MGI Ref ID J:139682]

Oshima T; Sonoda KH; Tsutsumi-Miyahara C; Qiao H; Hisatomi T; Nakao S; Hamano S; Egashira K; Charo IF; Ishibashi T. 2006. Analysis of corneal inflammation induced by cauterisation in CCR2 and MCP-1 knockout mice. Br J Ophthalmol 90(2):218-22. [PubMed: 16424537]  [MGI Ref ID J:135816]

Panoskaltsis-Mortari A; Hermanson JR; Taras E; Wangensteen OD; Charo IF; Rollins BJ; Blazar BR. 2004. Post-BMT lung injury occurs independently of the expression of CCL2 or its receptor, CCR2, on host cells. Am J Physiol Lung Cell Mol Physiol 286(2):L284-92. [PubMed: 14527928]  [MGI Ref ID J:101226]

Pattarini R; Smeyne RJ; Morgan JI. 2007. Temporal mRNA profiles of inflammatory mediators in the murine 1-methyl-4-phenyl-1,2,3,6-tetrahydropyrimidine model of Parkinson's disease. Neuroscience 145(2):654-68. [PubMed: 17258864]  [MGI Ref ID J:121648]

Pesce JT; Liu Z; Hamed H; Alem F; Whitmire J; Lin H; Liu Q; Urban JF Jr; Gause WC. 2008. Neutrophils clear bacteria associated with parasitic nematodes augmenting the development of an effective Th2-type response. J Immunol 180(1):464-74. [PubMed: 18097048]  [MGI Ref ID J:130915]

Rankine EL; Hughes PM; Botham MS; Perry VH; Felton LM. 2006. Brain cytokine synthesis induced by an intraparenchymal injection of LPS is reduced in MCP-1-deficient mice prior to leucocyte recruitment. Eur J Neurosci 24(1):77-86. [PubMed: 16882009]  [MGI Ref ID J:111522]

Remington LT; Babcock AA; Zehntner SP; Owens T. 2007. Microglial recruitment, activation, and proliferation in response to primary demyelination. Am J Pathol 170(5):1713-24. [PubMed: 17456776]  [MGI Ref ID J:121054]

Robben PM; Laregina M; Kuziel WA; Sibley LD. 2005. Recruitment of Gr-1+ monocytes is essential for control of acute toxoplasmosis. J Exp Med 201(11):1761-9. [PubMed: 15928200]  [MGI Ref ID J:99209]

Ross RJ; Zhou M; Shen D; Fariss RN; Ding X; Bojanowski CM; Tuo J; Chan CC. 2008. Immunological protein expression profile in Ccl2/Cx3cr1 deficient mice with lesions similar to age-related macular degeneration. Exp Eye Res 86(4):675-83. [PubMed: 18308304]  [MGI Ref ID J:135650]

Saito Y; Hasegawa M; Fujimoto M; Matsushita T; Horikawa M; Takenaka M; Ogawa F; Sugama J; Steeber DA; Sato S; Takehara K. 2008. The loss of MCP-1 attenuates cutaneous ischemia-reperfusion injury in a mouse model of pressure ulcer. J Invest Dermatol 128(7):1838-51. [PubMed: 18219277]  [MGI Ref ID J:137533]

Salomon R; Hoffmann E; Webster RG. 2007. Inhibition of the cytokine response does not protect against lethal H5N1 influenza infection. Proc Natl Acad Sci U S A 104(30):12479-81. [PubMed: 17640882]  [MGI Ref ID J:123301]

Serbina NV; Kuziel W; Flavell R; Akira S; Rollins B; Pamer EG. 2003. Sequential MyD88-independent and -dependent activation of innate immune responses to intracellular bacterial infection. Immunity 19(6):891-901. [PubMed: 14670305]  [MGI Ref ID J:86994]

Serbina NV; Pamer EG. 2006. Monocyte emigration from bone marrow during bacterial infection requires signals mediated by chemokine receptor CCR2. Nat Immunol 7(3):311-7. [PubMed: 16462739]  [MGI Ref ID J:112610]

Shireman PK; Contreras-Shannon V; Ochoa O; Karia BP; Michalek JE; McManus LM. 2007. MCP-1 deficiency causes altered inflammation with impaired skeletal muscle regeneration. J Leukoc Biol 81(3):775-85. [PubMed: 17135576]  [MGI Ref ID J:118606]

Sweeney EA; Lortat-Jacob H; Priestley GV; Nakamoto B; Papayannopoulou T. 2002. Sulfated polysaccharides increase plasma levels of SDF-1 in monkeys and mice: involvement in mobilization of stem/progenitor cells. Blood 99(1):44-51. [PubMed: 11756151]  [MGI Ref ID J:73665]

Tesch GH; Schwarting A; Kinoshita K; Lan HY; Rollins BJ; Kelley VR. 1999. Monocyte chemoattractant protein-1 promotes macrophage-mediated tubular injury, but not glomerular injury, in nephrotoxic serum nephritis. J Clin Invest 103(1):73-80. [PubMed: 9884336]  [MGI Ref ID J:119615]

Tsou CL; Peters W; Si Y; Slaymaker S; Aslanian AM; Weisberg SP; Mack M; Charo IF. 2007. Critical roles for CCR2 and MCP-3 in monocyte mobilization from bone marrow and recruitment to inflammatory sites. J Clin Invest 117(4):902-9. [PubMed: 17364026]  [MGI Ref ID J:121281]

Tuaillon N; Shen DF; Berger RB; Lu B; Rollins BJ; Chan CC. 2002. MCP-1 expression in endotoxin-induced uveitis. Invest Ophthalmol Vis Sci 43(5):1493-8. [PubMed: 11980865]  [MGI Ref ID J:119434]

Tuo J; Bojanowski CM; Zhou M; Shen D; Ross RJ; Rosenberg KI; Cameron DJ; Yin C; Kowalak JA; Zhuang Z; Zhang K; Chan CC. 2007. Murine ccl2/cx3cr1 deficiency results in retinal lesions mimicking human age-related macular degeneration. Invest Ophthalmol Vis Sci 48(8):3827-36. [PubMed: 17652758]  [MGI Ref ID J:126935]

Verma V; Sauer T; Chan CC; Zhou M; Zhang C; Maminishkis A; Shen D; Tuo J. 2008. Constancy of ERp29 expression in cultured retinal pigment epithelial cells in the Ccl2/Cx3cr1 deficient mouse model of age-related macular degeneration. Curr Eye Res 33(8):701-7. [PubMed: 18696346]  [MGI Ref ID J:140475]

Waeckel L; Mallat Z; Potteaux S; Combadiere C; Clergue M; Duriez M; Bao L; Gerard C; Rollins BJ; Tedgui A; Levy BI; Silvestre JS. 2005. Impairment in postischemic neovascularization in mice lacking the CXC chemokine receptor 3. Circ Res 96(5):576-82. [PubMed: 15718500]  [MGI Ref ID J:107646]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX8

Colony Maintenance

Breeding & Husbandry	The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for 10 generations. Coat color expected from breeding:Black
Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply	Level 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one to three months.
Supply Notes	Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available. Strains that must be genotyped are not available until five to seven weeks of age. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.