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- Genes & alleles
- Genotyping
- Health & husbandry
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- Purchasing information
Description
Strain Information
Former Names STOCK Tg(cre/Esr1)5Amc/J (Changed: 14-JUL-08 ) Type Mutant Stock; Transgenic; Mating System Heterozygote x +/+ sibling (Female x Male) Species laboratory mouse Donating Investigator Andrew McMahon, Harvard University Description
These transgenic mice have a tamoxifen-inducible cre-mediated recombination system driven by the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice. Homozygous mice are not viable or fertile. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.Development
A transgenic construct containing sequence encoding cre/Esr1 under the control of a chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer, was introduced into B6CBF1 donor eggs.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(CAG-cre/Esr1)5Amc allele
004682 B6.Cg-Tg(CAG-cre/Esr1)5Amc/J View Strains carrying Tg(CAG-cre/Esr1)5Amc (1 strain)
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 004744 B6.129P2-Esr1tm1Ksk/J 005657 B6.Cg-Tg(Myh6-cre/Esr1)1Jmk/J 005650 B6129-Tg(Myh6-cre/Esr1)1Jmk/J 006774 FVB-Tg(Col2a1-cre/ESR1)KA3Smac/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 008464 STOCK Foxa2tm2.1(cre/Esr1)Moon/J 008122 STOCK Tg(Ins2-cre/Esr1)1Dam/J 005107 STOCK Tg(KRT14-cre/Esr1)20Efu/J 008119 STOCK Tg(Neurog3-cre/Esr1)1Dam/J
Additional Web Information
Cre-lox Systems
Phenotype
Phenotype Information
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre-Recombinase Expression: Inducible)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-cre/Esr1)5Amc | ||
|---|---|---|---|
| Allele Name | transgene insertion 5, Andrew P McMahon | ||
| Common Name(s) | CAG-CreERT2; CAGG-CreERT2; CAGGCre-ER; CAGGCre-ERTM; CAGGCre-ERtm line 5.8; CAGGS-CreER; Cre-ER; Cre-ERTM; Tg(cre/Esr1)5Amc; | ||
| Mutation Made By | Shigemi Hayashi, Columbia University | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | tamoxifen-inducible cre; widespread pattern of expression; tamoxifen administration will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice | ||
| Expressed Gene | Esr1, estrogen receptor 1 (alpha), mouse, laboratory | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | ACTB, actin, beta, chicken | ||
| General Note |
Homozygous transgenic mice are not viable or fertile. Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In transgenic mice the mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 fusion protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing loxP sites flanking a sequence of interest, tamoxifen induced, Cre-mediated targeted deletions are generated in the offspring. Tamoxifen administration will induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice. | ||
| Molecular Note | This transgene expresses a fusion protein consisting of Cre recombinase joined to the ligand-binding domain of a mouse estrogen receptor modified to bind to 4-hydroxytamoxifen, but not to endogenous estrogen. The CAG promoter, containing a chicken beta actin promoter/enhancer coupled with the cytomegalovirus immediate-early (CMV-IE) enhancer, drives high levels of expression in most tissues. In the presence of tamoxifen, the fusion protein is transported into the nucleus, where Cre can excise loxP-flanked segments from conditionally modified genes. [MGI Ref ID J:76130] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Hayashi S; McMahon AP. 2002. Efficient recombination in diverse tissues by a tamoxifen-inducible form of cre: a tool for temporally regulated gene activation/inactivation in the mouse. Dev Biol 244(2):305-18. [PubMed: 11944939] [MGI Ref ID J:76130]
Additional References
Tg(CAG-cre/Esr1)5Amc related
Bhaskara S; Chyla BJ; Amann JM; Knutson SK; Cortez D; Sun ZW; Hiebert SW. 2008. Deletion of histone deacetylase 3 reveals critical roles in S phase progression and DNA damage control. Mol Cell 30(1):61-72. [PubMed: 18406327] [MGI Ref ID J:134665]Brenner-Anantharam A; Cebrian C; Guillaume R; Hurtado R; Sun TT; Herzlinger D. 2007. Tailbud-derived mesenchyme promotes urinary tract segmentation via BMP4 signaling. Development 134(10):1967-75. [PubMed: 17442697] [MGI Ref ID J:121412]
Cai J; Chen Y; Cai WH; Hurlock EC; Wu H; Kernie SG; Parada LF; Lu QR. 2007. A crucial role for Olig2 in white matter astrocyte development. Development 134(10):1887-99. [PubMed: 17428828] [MGI Ref ID J:121422]
Chang H; Gao F; Guillou F; Taketo MM; Huff V; Behringer RR. 2008. Wt1 negatively regulates {beta}-catenin signaling during testis development. Development 135(10):1875-85. [PubMed: 18403409] [MGI Ref ID J:134687]
Cheng J; Du J. 2007. Mechanical stretch simulates proliferation of venous smooth muscle cells through activation of the insulin-like growth factor-1 receptor. Arterioscler Thromb Vasc Biol 27(8):1744-51. [PubMed: 17541019] [MGI Ref ID J:134907]
Connerney J; Andreeva V; Leshem Y; Mercado MA; Dowell K; Yang X; Lindner V; Friesel RE; Spicer DB. 2008. Twist1 homodimers enhance FGF responsiveness of the cranial sutures and promote suture closure. Dev Biol 318(2):323-34. [PubMed: 18471809] [MGI Ref ID J:136965]
Gould TW; Yonemura S; Oppenheim RW; Ohmori S; Enomoto H. 2008. The neurotrophic effects of glial cell line-derived neurotrophic factor on spinal motoneurons are restricted to fusimotor subtypes. J Neurosci 28(9):2131-46. [PubMed: 18305247] [MGI Ref ID J:132854]
Guy J; Gan J; Selfridge J; Cobb S; Bird A. 2007. Reversal of neurological defects in a mouse model of Rett syndrome. Science 315(5815):1143-7. [PubMed: 17289941] [MGI Ref ID J:118365]
Herrmann S; Stieber J; Stockl G; Hofmann F; Ludwig A. 2007. HCN4 provides a 'depolarization reserve' and is not required for heart rate acceleration in mice. EMBO J 26(21):4423-32. [PubMed: 17914461] [MGI Ref ID J:139560]
Higgins DF; Kimura K; Bernhardt WM; Shrimanker N; Akai Y; Hohenstein B; Saito Y; Johnson RS; Kretzler M; Cohen CD; Eckardt KU; Iwano M; Haase VH. 2007. Hypoxia promotes fibrogenesis in vivo via HIF-1 stimulation of epithelial-to-mesenchymal transition. J Clin Invest 117(12):3810-20. [PubMed: 18037992] [MGI Ref ID J:130751]
Knapp JR; Davie JK; Myer A; Meadows E; Olson EN; Klein WH. 2006. Loss of myogenin in postnatal life leads to normal skeletal muscle but reduced body size. Development 133(4):601-10. [PubMed: 16407395] [MGI Ref ID J:105925]
Kobayashi T; Lu J; Cobb BS; Rodda SJ; McMahon AP; Schipani E; Merkenschlager M; Kronenberg HM. 2008. Dicer-dependent pathways regulate chondrocyte proliferation and differentiation. Proc Natl Acad Sci U S A 105(6):1949-54. [PubMed: 18238902] [MGI Ref ID J:131828]
Li G; Adesnik H; Li J; Long J; Nicoll RA; Rubenstein JL; Pleasure SJ. 2008. Regional distribution of cortical interneurons and development of inhibitory tone are regulated by Cxcl12/Cxcr4 signaling. J Neurosci 28(5):1085-98. [PubMed: 18234887] [MGI Ref ID J:131835]
Liu W; Lagutin OV; Mende M; Streit A; Oliver G. 2006. Six3 activation of Pax6 expression is essential for mammalian lens induction and specification. EMBO J 25(22):5383-95. [PubMed: 17066077] [MGI Ref ID J:116102]
Mao J; Ligon KL; Rakhlin EY; Thayer SP; Bronson RT; Rowitch D; McMahon AP. 2006. A novel somatic mouse model to survey tumorigenic potential applied to the Hedgehog pathway. Cancer Res 66(20):10171-8. [PubMed: 17047082] [MGI Ref ID J:114992]
Minamishima YA; Moslehi J; Bardeesy N; Cullen D; Bronson RT; Kaelin WG Jr. 2008. Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure. Blood 111(6):3236-44. [PubMed: 18096761] [MGI Ref ID J:132718]
Mudhasani R; Zhu Z; Hutvagner G; Eischen CM; Lyle S; Hall LL; Lawrence JB; Imbalzano AN; Jones SN. 2008. Loss of miRNA biogenesis induces p19Arf-p53 signaling and senescence in primary cells. J Cell Biol 181(7):1055-63. [PubMed: 18591425] [MGI Ref ID J:139257]
Pascual A; Hidalgo-Figueroa M; Piruat JI; Pintado CO; Gomez-Diaz R; Lopez-Barneo J. 2008. Absolute requirement of GDNF for adult catecholaminergic neuron survival. Nat Neurosci 11(7):755-61. [PubMed: 18536709] [MGI Ref ID J:139371]
Penzo-Mendez A; Dy P; Pallavi B; Lefebvre V. 2007. Generation of mice harboring a Sox4 conditional null allele. Genesis 45(12):776-80. [PubMed: 18064674] [MGI Ref ID J:130461]
Perera D; Tilston V; Hopwood JA; Barchi M; Boot-Handford RP; Taylor SS. 2007. Bub1 maintains centromeric cohesion by activation of the spindle checkpoint. Dev Cell 13(4):566-79. [PubMed: 17925231] [MGI Ref ID J:128455]
Quignodon L; Vincent S; Winter H; Samarut J; Flamant F. 2007. A Point Mutation in the Activation Function 2 Domain of Thyroid Hormone Receptor {alpha}1 Expressed after CRE-Mediated Recombination Partially Recapitulates Hypothyroidism. Mol Endocrinol 21(10):2350-2360. [PubMed: 17622582] [MGI Ref ID J:125353]
Regard JB; Kataoka H; Cano DA; Camerer E; Yin L; Zheng YW; Scanlan TS; Hebrok M; Coughlin SR. 2007. Probing cell type-specific functions of Gi in vivo identifies GPCR regulators of insulin secretion. J Clin Invest 117(12):4034-43. [PubMed: 17992256] [MGI Ref ID J:130781]
Uesaka T; Jain S; Yonemura S; Uchiyama Y; Milbrandt J; Enomoto H. 2007. Conditional ablation of GFRalpha1 in postmigratory enteric neurons triggers unconventional neuronal death in the colon and causes a Hirschsprung's disease phenotype. Development 134(11):2171-81. [PubMed: 17507417] [MGI Ref ID J:122607]
Uesaka T; Nagashimada M; Yonemura S; Enomoto H. 2008. Diminished Ret expression compromises neuronal survival in the colon and causes intestinal aganglionosis in mice. J Clin Invest 118(5):1890-8. [PubMed: 18414682] [MGI Ref ID J:135153]
Wang M; Crisostomo P; Wairiuko GM; Meldrum DR. 2006. Estrogen receptor-alpha mediates acute myocardial protection in females. Am J Physiol Heart Circ Physiol 290(6):H2204-9. [PubMed: 16415070] [MGI Ref ID J:111852]
Wang P; Bowl MR; Bender S; Peng J; Farber L; Chen J; Ali A; Zhang Z; Alberts AS; Thakker RV; Shilatifard A; Williams BO; Teh BT. 2008. Parafibromin, a component of the human PAF complex, regulates growth factors and is required for embryonic development and survival in adult mice. Mol Cell Biol 28(9):2930-40. [PubMed: 18212049] [MGI Ref ID J:135255]
Welle S; Bhatt K; Pinkert CA; Tawil R; Thornton CA. 2007. Muscle growth after postdevelopmental myostatin gene knockout. Am J Physiol Endocrinol Metab 292(4):E985-91. [PubMed: 17148752] [MGI Ref ID J:121233]
Xu H; Cerrato F; Baldini A. 2005. Timed mutation and cell-fate mapping reveal reiterated roles of Tbx1 during embryogenesis, and a crucial function during segmentation of the pharyngeal system via regulation of endoderm expansion. Development 132(19):4387-95. [PubMed: 16141220] [MGI Ref ID J:127213]
Xu H; Chen L; Baldini A. 2007. In vivo genetic ablation of the periotic mesoderm affects cell proliferation survival and differentiation in the cochlea. Dev Biol 310(2):329-40. [PubMed: 17825816] [MGI Ref ID J:128008]
Xu H; Viola A; Zhang Z; Gerken CP; Lindsay-Illingworth EA; Baldini A. 2007. Tbx1 regulates population, proliferation and cell fate determination of otic epithelial cells. Dev Biol 302(2):670-82. [PubMed: 17074316] [MGI Ref ID J:119950]
Yang B; Zhao D; Qian L; Verkman AS. 2006. Mouse model of inducible nephrogenic diabetes insipidus produced by floxed aquaporin-2 gene deletion. Am J Physiol Renal Physiol 291(2):F465-72. [PubMed: 16434568] [MGI Ref ID J:110648]
Yang X; Harkins LK; Zubanova O; Harrington A; Kovalenko D; Nadeau RJ; Chen PY; Toher JL; Lindner V; Liaw L; Friesel R. 2008. Overexpression of Spry1 in chondrocytes causes attenuated FGFR ubiquitination and sustained ERK activation resulting in chondrodysplasia. Dev Biol 321(1):64-76. [PubMed: 18582454] [MGI Ref ID J:138616]
Yuan X; Yao J; Norris D; Tran DD; Bram RJ; Chen G; Luscher B. 2008. Calcium-modulating cyclophilin ligand regulates membrane trafficking of postsynaptic GABA(A) receptors. Mol Cell Neurosci 38(2):277-89. [PubMed: 18424167] [MGI Ref ID J:136913]
Zhang CL; Zou Y; He W; Gage FH; Evans RM. 2008. A role for adult TLX-positive neural stem cells in learning and behaviour. Nature 451(7181):1004-7. [PubMed: 18235445] [MGI Ref ID J:132664]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry The resulting male transgenic mouse was crossed with a mixed (129, B6 and Swiss Webster) female. Male progeny from this cross were mated to Swiss Webster female mice twice, before being crossed once to SWR/J and then C57BL/6J. When held in a live colony, this strain is maintained as a hemizygote. Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Notice Regarding cre/Esr1 (Tamoxifen Inducible Cre) Strains.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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