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| NONcNZO10/LtJ is a recombinant congenic strain developed at The Jackson Laboratory to model human obesity-induced Type 2 diabetes and Metabolic Syndrome. Type 2 diabetes in males of this strain results from polygenic interactions producing a moderate obesity rather than the massive obesity elicited by mutations in the leptin/leptin receptor axis. Unlike mice with monogenic obesity syndromes, NONcNZO10/LtJ males do not exhibit hypercorticism, are not hyperphagic, and show no obvious thermoregulatory defects. Male mice weaned onto LabDiet® 5K20 (a chow diet containing 10-11% fat by weight) develop visceral obesity, maturity-onset hyperglycemia, dyslipidemia, moderate liver steatosis, and pancreatic islet atrophy. | |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names RC-10 (Changed: 15-DEC-04 ) Type Recombinant Congenic (Rc); Additional information on Recombinant Congenic Mice. Mating System Sibling x Sibling (Female x Male) Species laboratory mouse Background Strain NON/ShiLtJ Donor Strain NZO/HlLt H2 Haplotype nb1 Generation N1F11+8 (16-NOV-05) Donating Investigator Edward Leiter, The Jackson Laboratory 
Appearance
albino
Related Genotype: A/A Tyrc/TyrcDescription
Onset of hyperglycemia occurs between 10-12 on a 10-11% fat (wt/wt) chow diet with greater than 85% diabetic by 18 weeks. Males exhibit increased serum triglycerides, moderate to severe liver steatosis and pancreatic islet atrophy similar to NZO/HlLt males. Serum insulin and leptin values are significantly lower than in NZO/HlLt, and are only moderately elevated above those recorded in NON/ShiLtJ males. Unlike the very obese NZO/HlLt mice, NONcNZO10/LtJ mice do not exhibit hyperphagia or hypercorticism and are much easier to breed. Although NONcNZO10/LtJ males develop only a moderate level of obesity compared to NZO/HlLt males, the interaction with known diabetogenic QTL from the NON/ShiLtJ strain produce an earlier onset and higher prevalence of chronic hyperglycemia than observed in NZO/HlLt males.NONcNZO10/LtJ is differentially sensitive to adverse hepatic side effects of thiazolidinediones and may be useful for pharmac ogenetic analysis. This strain represents a model of polygenic obesity and obesity-induced diabetes (diabesity).
Note that the diabetes phenotype originally characterized by Dr. Leiter in his research vivarium was performed with mice fed a 6% wt/wt diet. After the strain was rederived it was experimentally determined that it is essential to feed the mice a higher fat diet to force penetrance of the diabetes phenotype.
Development
A recombinant congenic developed by introgressing six known diabesity QTL from the Type 2 diabetes-prone and obese NZO/HlLt inbred strain into the nominally nonobese and diabetes-resistant NON/Lt strain background. NZO-derived QTL include those marked by D1Mit411, D5Mit7, D11Mit261/D11Mit41, D12Mit231 and D15Mit159.
Control Information
| Control | ||
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| Please Inquire | The controls recommended for NONcNZO10/LtJ depend on its intended use. The parental line, NON/ShiLtJ (Stock No. 002423), provides a genetic background control (88% homology). On a 10-11% fat diet, which is available for purchase, this strain exhibits moderate obesity and impaired glucose tolerance, but does not develop hyperglycemia. Like NON/ShiLtJ, SWR/J (Stock No. 000689) is a Swiss-derived inbred. SWR/J mice are resistant to diet-induced obesity and do not exhibit impaired glucose tolerance. A third option, NONcNZO5/LtJ (Stock No. 004455), is available only in limited quantities, however, males of this strain exhibit moderate obesity, and both sexes are diabetes resistant. | |
| This strain is under active development and we would be happy to share unpublished data and work with investigators interested in working with this strain to assist them in the selection of a control appropriate for their study. | ||
| Considerations for Choosing Controls | ||
Related Strains
NONcNZO Strains
003668 NONcNZO1/Lt 003670 NONcNZO3/Lt 003671 NONcNZO4/Lt 004455 NONcNZO5/LtJ 003673 NONcNZO6/Lt 003675 NONcNZO8/Lt View NONcNZO Strains (6 strains)
Additional Web Information
Genetic Quality Control Annual Report
JAX® NOTES, Fall 2002; 487. New Polygenic Obesity Mouse Models.
JAX® NOTES, Spring 2005; 497. NONcNZO10/LtJ, a New JAX® Mice Model for Obesity-Induced Type 2 Diabetes
Phenotype
Phenotype Information
Mouse Phenome Database
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Hyperglycemia (males)
Obesity With Diabetes (moderate, males)
Obesity Without Diabetes (females)
Type 2 Diabetes (NIDDM) (males)
Genes & Alleles
Gene & Allele Information
Currently there are no related genes or alleles for this strain.Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Leiter EH; Reifsnyder PC. 2004. Differential levels of diabetogenic stress in two new mouse models of obesity and type 2 diabetes. Diabetes 53 Suppl 1:S4-11. [PubMed: 14749259] [MGI Ref ID J:109841]
Leiter EH; Reifsnyder PC; Flurkey K; Partke HJ; Junger E; Herberg L. 1998. NIDDM genes in mice: deleterious synergism by both parental genomes contributes to diabetogenic thresholds. Diabetes 47(8):1287-95. [PubMed: 9703330] [MGI Ref ID J:48957]
Reifsnyder PC; Churchill G; Leiter EH. 2000. Maternal environment and genotype interact to establish diabesity in mice. Genome Res 10(10):1568-78. [PubMed: 11042154] [MGI Ref ID J:65256]
Reifsnyder PC; Leiter EH. 2002. Deconstructing and reconstructing obesity-induced diabetes (diabesity) in mice. Diabetes 51(3):825-32. [PubMed: 11872687] [MGI Ref ID J:75024]
Taylor BA; Wnek C; Schroeder D; Phillips SJ. 2001. Multiple obesity QTLs identified in an intercross between the NZO (New Zealand obese) and the SM (small) mouse strains. Mamm Genome 12(2):95-103. [PubMed: 11210195] [MGI Ref ID J:67838]
Watkins SM; Reifsnyder PR; Pan HJ; German JB; Leiter EH. 2002. Lipid metabolome-wide effects of the PPARgamma agonist rosiglitazone. J Lipid Res 43(11):1809-17. [PubMed: 12401879] [MGI Ref ID J:109849]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX1
Colony Maintenance
Mating System Sibling x Sibling (Female x Male) Diet Information LabDiet® 5K20
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender 4-5 weeks $74.40 Female or Male 6 weeks $78.30 Female or Male 7 weeks $82.20 Female or Male 8 weeks $86.10 Female or Male 9 weeks $90.00 Female or Male 10 weeks $93.90 Female or Male 11 weeks $97.80 Female or Male 12 weeks $101.70 Female or Male
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender 4-5 weeks $96.80 Female or Male 6 weeks $101.90 Female or Male 7 weeks $107.00 Female or Male 8 weeks $112.10 Female or Male 9 weeks $117.20 Female or Male 10 weeks $122.30 Female or Male 11 weeks $127.40 Female or Male 12 weeks $132.50 Female or Male
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Level 2. Up to 100 mice. Larger quantities or custom orders arranged upon request. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Please Inquire | The controls recommended for NONcNZO10/LtJ depend on its intended use. The parental line, NON/ShiLtJ (Stock No. 002423), provides a genetic background control (88% homology). On a 10-11% fat diet, which is available for purchase, this strain exhibits moderate obesity and impaired glucose tolerance, but does not develop hyperglycemia. Like NON/ShiLtJ, SWR/J (Stock No. 000689) is a Swiss-derived inbred. SWR/J mice are resistant to diet-induced obesity and do not exhibit impaired glucose tolerance. A third option, NONcNZO5/LtJ (Stock No. 004455), is available only in limited quantities, however, males of this strain exhibit moderate obesity, and both sexes are diabetes resistant. | |
| This strain is under active development and we would be happy to share unpublished data and work with investigators interested in working with this strain to assist them in the selection of a control appropriate for their study. | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Notice regarding NONcNZO10/LtJ Female
- Notice regarding NONcNZO10/LtJ Male
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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