Description

Strain Information

Former Names RC-10    (Changed: 15-DEC-04 ) Type Recombinant Congenic (RC); Additional information on Recombinant Congenic Mice. Visit our online Nomenclature tutorial. Mating System Sibling x Sibling         (Female x Male)   01-MAR-06 Species laboratory mouse Background Strain NON/ShiLtJ Donor Strain NZO/HlLt H2 Haplotype nb1 Generation N1F11+8 (16-NOV-05)   Donating Investigator Edward Leiter,   The Jackson Laboratory

View larger image

Appearance
albino
Related Genotype: A/A Tyr^c/Tyr^c

Description
Onset of hyperglycemia occurs between 10-12 on a 10-11% fat (wt/wt) chow diet with greater than 85% diabetic by 18 weeks. Males exhibit increased serum triglycerides, moderate to severe liver steatosis and pancreatic islet atrophy similar to NZO/HlLt males. Serum insulin and leptin values are significantly lower than in NZO/HlLt, and are only moderately elevated above those recorded in NON/ShiLtJ males. Unlike the very obese NZO/HlLt mice, NONcNZO10/LtJ mice do not exhibit hyperphagia or hypercorticism and are much easier to breed. Although NONcNZO10/LtJ males develop only a moderate level of obesity compared to NZO/HlLt males, the interaction with known diabetogenic QTL from the NON/ShiLtJ strain produce an earlier onset and higher prevalence of chronic hyperglycemia than observed in NZO/HlLt males.

NONcNZO10/LtJ is differentially sensitive to adverse hepatic side effects of thiazolidinediones and may be useful for pharmac ogenetic analysis. This strain represents a model of polygenic obesity and obesity-induced diabetes (diabesity).

Note that the diabetes phenotype originally characterized by Dr. Leiter in his research vivarium was performed with mice fed a 6% wt/wt diet. After the strain was rederived it was experimentally determined that it is essential to feed the mice a higher fat diet to force penetrance of the diabetes phenotype.

Development
A recombinant congenic developed by introgressing six known diabesity QTL from the Type 2 diabetes-prone and obese NZO/HlLt inbred strain into the nominally nonobese and diabetes-resistant NON/Lt strain background. NZO-derived QTL include those marked by D1Mit411, D5Mit7, D11Mit261/D11Mit41, D12Mit231 and D15Mit159.

Control Information

	Control
	Please Inquire	The controls recommended for NONcNZO10/LtJ depend on its intended use. The parental line, NON/ShiLtJ (Stock No. 002423), provides a genetic background control (88% homology). On a 10-11% fat diet, which is available for purchase, this strain exhibits moderate obesity and impaired glucose tolerance, but does not develop hyperglycemia. Like NON/ShiLtJ, SWR/J (Stock No. 000689) is a Swiss-derived inbred. SWR/J mice are resistant to diet-induced obesity and do not exhibit impaired glucose tolerance. A third option, NONcNZO5/LtJ (Stock No. 004455), is available only in limited quantities, however, males of this strain exhibit moderate obesity, and both sexes are diabetes resistant.
		This strain is under active development and we would be happy to share unpublished data and work with investigators interested in working with this strain to assist them in the selection of a control appropriate for their study.
Considerations for Choosing Controls

Related Strains

NONcNZO Strains

003668	NONcNZO1/Lt
003670	NONcNZO3/Lt
003671	NONcNZO4/Lt
004455	NONcNZO5/LtJ
003673	NONcNZO6/Lt
003675	NONcNZO8/Lt

View NONcNZO Strains     (6 strains)

Additional Web Information

Genetic Quality Control Annual Report
JAX^® NOTES, Fall 2002; 487. New Polygenic Obesity Mouse Models.
JAX^® NOTES, Spring 2005; 497. NONcNZO10/LtJ, a New JAX^® Mice Model for Obesity-Induced Type 2 Diabetes

Phenotype

Phenotype Information

View Phenotypic Data

Phenotypic Data

Mouse Phenome Database

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Hyperglycemia
      males
Obesity With Diabetes
      moderate, males
Obesity Without Diabetes
      females
Type 2 Diabetes (NIDDM)
      males

Genes & Alleles

Gene & Allele Information

Currently there are no related genes or alleles for this strain.

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Leiter EH; Reifsnyder PC. 2004. Differential levels of diabetogenic stress in two new mouse models of obesity and type 2 diabetes. Diabetes 53 Suppl 1:S4-11. [PubMed: 14749259]  [MGI Ref ID J:109841]

Leiter EH; Reifsnyder PC; Flurkey K; Partke HJ; Junger E; Herberg L. 1998. NIDDM genes in mice: deleterious synergism by both parental genomes contributes to diabetogenic thresholds. Diabetes 47(8):1287-95. [PubMed: 9703330]  [MGI Ref ID J:48957]

Reifsnyder PC; Churchill G; Leiter EH. 2000. Maternal environment and genotype interact to establish diabesity in mice. Genome Res 10(10):1568-78. [PubMed: 11042154]  [MGI Ref ID J:65256]

Reifsnyder PC; Leiter EH. 2002. Deconstructing and reconstructing obesity-induced diabetes (diabesity) in mice. Diabetes 51(3):825-32. [PubMed: 11872687]  [MGI Ref ID J:75024]

Taylor BA; Wnek C; Schroeder D; Phillips SJ. 2001. Multiple obesity QTLs identified in an intercross between the NZO (New Zealand obese) and the SM (small) mouse strains. Mamm Genome 12(2):95-103. [PubMed: 11210195]  [MGI Ref ID J:67838]

Watkins SM; Reifsnyder PR; Pan HJ; German JB; Leiter EH. 2002. Lipid metabolome-wide effects of the PPARgamma agonist rosiglitazone. J Lipid Res 43(11):1809-17. [PubMed: 12401879]  [MGI Ref ID J:109849]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX1

Colony Maintenance

Mating System	Sibling x Sibling         (Female x Male)   01-MAR-06
Diet Information	LabDiet® 5K20

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply	Level 2. Up to 100 mice. Larger quantities or custom orders arranged upon request.
Supply Notes	Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available. Strains that must be genotyped are not available until five to seven weeks of age. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Please Inquire	The controls recommended for NONcNZO10/LtJ depend on its intended use. The parental line, NON/ShiLtJ (Stock No. 002423), provides a genetic background control (88% homology). On a 10-11% fat diet, which is available for purchase, this strain exhibits moderate obesity and impaired glucose tolerance, but does not develop hyperglycemia. Like NON/ShiLtJ, SWR/J (Stock No. 000689) is a Swiss-derived inbred. SWR/J mice are resistant to diet-induced obesity and do not exhibit impaired glucose tolerance. A third option, NONcNZO5/LtJ (Stock No. 004455), is available only in limited quantities, however, males of this strain exhibit moderate obesity, and both sexes are diabetes resistant.
		This strain is under active development and we would be happy to share unpublished data and work with investigators interested in working with this strain to assist them in the selection of a control appropriate for their study.
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Notice regarding NONcNZO10/LtJ Female
- Notice regarding NONcNZO10/LtJ Male

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.