Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names Clock gene BAC transgenic overexpression    (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N8+N2pN1 Generation Definitions   Donating Investigator Joseph Takahashi,   Northwestern University

Description
Mice hemizygous for this transgene, a 140kb circular BAC 54 clone of the targeted gene, express 3-4 times more gene transcript than the wildtype. Hemizygous mice are viable, fertile, normal in size and do not display any gross physical abnormalities. Quantification of transgene copy number was estimated from Southern blot analysis. Hemizygotes exhibit a circadian period that is shorter than the wildtype. This transgene rescues the homozygous mutant Clock phenotype. This mutant mouse strain may be useful in studies of advanced sleep phase syndrome.

Development
A transgenic construct consisting of a 140kb circular BAC 54 clone of the targeted gene was injected into fertilized oocytes that were a mix of CD1, C57BL/6J and BALB/cJ. Founder mice were intercrossed for four generations before transgenic females were backcrossed to C57BL/6 males to establish a congenic line.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tg(BAC54)36Jt/0

        involves: BALB/cJ * C57BL/6J * CD-1

• behavior/neurological phenotype
• shortened circadian period (MGI Ref ID J:40363)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(BAC54)36Jt
Allele Name		transgene insertion 36, Joseph S Takahashi
Allele Type		Transgenic (random, expressed)
Common Name(s)		Clock gene BAC;
Mutation Made By		Joseph Takahashi,   Northwestern University
Strain of Origin		CD-1 x (BALB/cJ x C57BL/6J)F2
Expressed Gene		Clock, clock homolog (mouse), rat
Promoter		Clock, clock homolog (mouse), rat
Molecular Note		The transgene consists of a 140kb circular BAC 54 clone containing the Clock gene (circadian locomoter output cycles kaput), and includes the Clock promoter. [MGI Ref ID J:40363]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Bac54)36jt, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Antoch MP; Song EJ; Chang AM; Vitaterna MH; Zhao Y; Wilsbacher LD; Sangoram AM; King DP; Pinto LH; Takahashi JS. 1997. Functional identification of the mouse circadian Clock gene by transgenic BAC rescue. Cell 89(4):655-67. [PubMed: 9160756]  [MGI Ref ID J:40363]

Additional References

Gekakis N; Staknis D; Nguyen HB; Davis FC; Wilsbacher LD; King DP; Takahashi JS; Weitz CJ. 1998. Role of the CLOCK protein in the mammalian circadian mechanism [see comments] Science 280(5369):1564-9. [PubMed: 9616112]  [MGI Ref ID J:48067]

King DP; Zhao Y; Sangoram AM; Wilsbacher LD; Tanaka M; Antoch MP; Steeves TD; Vitaterna MH; Kornhauser JM; Lowrey PL; Turek FW; Takahashi JS. 1997. Positional cloning of the mouse circadian clock gene. Cell 89(4):641-53. [PubMed: 9160755]  [MGI Ref ID J:40364]

Sangoram AM; Saez L; Antoch MP; Gekakis N; Staknis D; Whiteley A; Fruechte EM; Vitaterna MH; Shimomura K; King DP; Young MW; Weitz CJ; Takahashi JS. 1998. Mammalian circadian autoregulatory loop: a timeless ortholog and mPer1 interact and negatively regulate CLOCK-BMAL1-induced transcription. Neuron 21(5):1101-13. [PubMed: 9856465]  [MGI Ref ID J:51392]

Vitaterna MH; King DP; Chang AM; Kornhauser JM; Lowrey PL; McDonald JD; Dove WF; Pinto LH; Turek FW; Takahashi JS. 1994. Mutagenesis and mapping of a mouse gene, Clock, essential for circadian behavior. Science 264(5159):719-25. [PubMed: 8171325]  [MGI Ref ID J:18005]

Tg(BAC54)36Jt related

Gekakis N; Staknis D; Nguyen HB; Davis FC; Wilsbacher LD; King DP; Takahashi JS; Weitz CJ. 1998. Role of the CLOCK protein in the mammalian circadian mechanism [see comments] Science 280(5369):1564-9. [PubMed: 9616112]  [MGI Ref ID J:48067]

Heaney JD; Bronson SK. 2006. Artificial chromosome-based transgenes in the study of genome function. Mamm Genome 17(8):791-807. [PubMed: 16897340]  [MGI Ref ID J:111659]

Herzog ED; Takahashi JS; Block GD. 1998. Clock controls circadian period in isolated suprachiasmatic nucleus neurons. Nat Neurosci 1(8):708-13. [PubMed: 10196587]  [MGI Ref ID J:51278]

King DP; Vitaterna MH; Chang AM; Dove WF; Pinto LH; Turek FW ; Takahashi JS. 1997. The mouse Clock mutation behaves as an antimorph and maps within the W19H deletion, distal of Kit. Genetics 146(3):1049-60. [PubMed: 9215907]  [MGI Ref ID J:41383]

King DP; Zhao Y; Sangoram AM; Wilsbacher LD; Tanaka M; Antoch MP; Steeves TD; Vitaterna MH; Kornhauser JM; Lowrey PL; Turek FW; Takahashi JS. 1997. Positional cloning of the mouse circadian clock gene. Cell 89(4):641-53. [PubMed: 9160755]  [MGI Ref ID J:40364]

Sangoram AM; Saez L; Antoch MP; Gekakis N; Staknis D; Whiteley A; Fruechte EM; Vitaterna MH; Shimomura K; King DP; Young MW; Weitz CJ; Takahashi JS. 1998. Mammalian circadian autoregulatory loop: a timeless ortholog and mPer1 interact and negatively regulate CLOCK-BMAL1-induced transcription. Neuron 21(5):1101-13. [PubMed: 9856465]  [MGI Ref ID J:51392]

Steeves TD; King DP; Zhao Y; Sangoram AM; Du F; Bowcock AM; Moore RY ; Takahashi JS. 1999. Molecular cloning and characterization of the human CLOCK gene: expression in the suprachiasmatic nuclei. Genomics 57(2):189-200. [PubMed: 10198158]  [MGI Ref ID J:54489]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	The resulting founder animals were crossed to C57BL/6 mice, and then backcrossed to the same for 8 generations.
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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