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Description
Strain Information
Former Names BDC2.5/Rag (Changed: 15-DEC-04 ) NOD.Cg-Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi Rag1tm1Mom/DoiJ (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Hemizygote x +/+ sibling (Female x Male) Species laboratory mouse Background Strain NOD/LtSz Donor Strain 129S7 via AB1 ES cell line Generation N11F17 (03-OCT-08) Donating Investigator Christophe Benoist, Joslin Diabetes Center Appearance
albino
Related Genotype: Tyrc/Tyrc
albino, pink eyed
Related Genotype: A/A Tyrc/TyrcDescription
NOD.Cg-Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi/DoiJ mice carry both rearranged TCR alpha and beta genes from the cytotoxic CD4+ T cell clone BDC-2.5. When paired with a homozygous Rag1tm1Mom mutation (such as in Stock No. 003729), recombination of endogenous TCR and Ig is prevented so that mature T cells in these mice express only the BDC2.5 TCR. On the NOD background, mice carrying the transgenes have a reduced incidence of diabetes relative to NOD/ShiLtJ controls (12% incidence at age 30 weeks). When coupled with the homozygous Rag1tm1Mom mutation, mice develop diabetes extremely early (mean age of 25 days). (Katz et al 1993, Gonzalez et al 2001, Mombaerts et al 1992)Development
This strain carries the rearranged T cell receptor genes Tcra and Tcrb from the diabetogenic H2-Ag7 restricted BDC2.5 Cd4+ T cell clone. The BDC2.5 Tcra and Tcrb sequences were co-injected into (B6xSJL)F2 eggs. To achieve the natural expression of Tcra, the rearranged ValphaJalpha sequence from BDC2.5 was cloned into the cassette vector generated by Kouskoff et al (1995), which contains both the 5' upstream promoter and the 3' downstream enhancer regions of the Tcra gene. The BDC2.5 Tcrb VbDbJb sequence, along with its 5' regulatory sequences and 3' enhancer region, was cloned into the construct generated by Signorelli et al upstream of the Cb2 constant region. Transgenic mice were bred to NOD/Lt for over 10 generations, resulting in the NOD/Lt-Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi strain. Through in situ hybridization, the integration site for these BDC2.5Tcr transgenes was localized to chromosome 13 near the D13mit125 marker. (Signorelli et al 1995; Kouskoff et al 1995 Katz et al 1993)
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Tcra
View Strains carrying other alleles of Tcra (35 strains)
Additional Web Information
Congenic Nomenclature
Phenotype
Phenotype Information
assigned by genotype
Tg(TcraBDC2.5)1Doi/0 Tg(TcrbBDC2.5)2Doi/0
NOD.Cg-Tg(TcraBDC2.5)1Doi Tg(TcrbBDC2.5)2Doi
- immune system phenotype
- decreased susceptibility to autoimmune diabetes (MGI Ref ID J:87251)
- adoptive tranfer of splenocytes from transgenic mice carrying the Tg(TcraBDC2.5)1Doi transgene into knockout recipients led to diabetes after a significant delay compared to diabetes development in wild-type recipients (100% in wild-type by 12 days versus 100% in knockouts by 20 days)
- increased activated T cell number (MGI Ref ID J:52940)
- in transgenic mice, a large proportion of cells in the pancreatic lymph nodes and pancreatic islets have an activated phenotype (CD4+ Vbeta4+) compared to nontransgenic littermates
- insulitis (MGI Ref ID J:52940)
- insulitis begins abruptly between days 15 and 18 after birth; at 14 days after birth, few activated (CD69+) T cells are detected
- digestive/alimentary phenotype
- insulitis (MGI Ref ID J:52940)
- insulitis begins abruptly between days 15 and 18 after birth; at 14 days after birth, few activated (CD69+) T cells are detected
- endocrine/exocrine gland phenotype
- insulitis (MGI Ref ID J:52940)
- insulitis begins abruptly between days 15 and 18 after birth; at 14 days after birth, few activated (CD69+) T cells are detected
- hematopoietic system phenotype
- increased activated T cell number (MGI Ref ID J:52940)
- in transgenic mice, a large proportion of cells in the pancreatic lymph nodes and pancreatic islets have an activated phenotype (CD4+ Vbeta4+) compared to nontransgenic littermates
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(TcraBDC2.5)1Doi/0 Tg(TcrbBDC2.5)2Doi/0
involves: C57BL/6 * NOD * SJLThis mouse can be used to support research in many areas including:
Tcra relatedDiabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Congenics with Mutations Affecting Immunocompetence)
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Immunology and Inflammation Research
Autoimmunity
Tcrb relatedHematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Research Tools
Cancer Research (specific T cell deficiency)
Hematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(TcraBDC2.5)1Doi | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Christophe Benoist | ||
| Allele Type | Transgenic (random, expressed) | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Expressed Gene | Tcra, T-cell receptor alpha chain, mouse, laboratory | ||
| Molecular Note | The construct consists of an expression cassette containing a rearranged Tcra sequence (V alpha J alpha) from diabetogenic T-cell clone BDC2.5. The sequence begins 20 bp 5' of the ATG site and ends 30 bp 3' of the slpice donor sequence of J alpha 17. [MGI Ref ID J:77007] | ||
| Allele Symbol | Tg(TcrbBDC2.5)2Doi | ||
| Allele Name | transgene insertion 2, Christophe Benoist | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Tg(TcrbBDC2.5)1Doi; | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Expressed Gene | Tcrb, T-cell receptor beta chain, mouse, laboratory | ||
| Molecular Note | The construct contains 6 kb of rearranged Tcrb sequence (V beta D beta J beta) from diabetogenic T-cell clone BDC2.5. This sequence is flanked by 2 kb of regulatory sequences and 3.5 kb of 3' DNA containing the unrearranged J beta 1.3 to J beta 1.6 sequences, and precedes a 12 kb sequence containing the C beta 2 region. [MGI Ref ID J:77007] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(TcraBDC2.5)1Doi, Tg(TcrbBDC2.5)2Doi, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Katz JD; Wang B; Haskins K; Benoist C; Mathis D. 1993. Following a diabetogenic T cell from genesis through pathogenesis. Cell 74(6):1089-100. [PubMed: 8402882] [MGI Ref ID J:77007]
Additional References
Gonzalez A; Andre-Schmutz I; Carnaud C; Mathis D; Benoist C. 2001. Damage control, rather than unresponsiveness, effected by protective DX5+ T cells in autoimmune diabetes. Nat Immunol 2(12):1117-25. [PubMed: 11713466] [MGI Ref ID J:109860]
Mombaerts P; Iacomini J; Johnson RS; Herrup K; Tonegawa S; Papaioannou VE. 1992. RAG-1-deficient mice have no mature B and T lymphocytes. Cell 68(5):869-77. [PubMed: 1547488] [MGI Ref ID J:1934]
Tg(TcraBDC2.5)1Doi relatedTg(TcrbBDC2.5)2Doi relatedAndre-Schmutz I; Hindelang C; Benoist C; Mathis D. 1999. Cellular and molecular changes accompanying the progression from insulitis to diabetes. Eur J Immunol 29(1):245-55. [PubMed: 9933106] [MGI Ref ID J:52915]
Balasa B; La Cava A; Van Gunst K; Mocnik L; Balakrishna D; Nguyen N; Tucker L; Sarvetnick N. 2000. A mechanism for IL-10-mediated diabetes in the nonobese diabetic (NOD) mouse: ICAM-1 deficiency blocks accelerated diabetes J Immunol 165(12):7330-7. [PubMed: 11120869] [MGI Ref ID J:66103]
Beaudoin L; Laloux V; Novak J; Lucas B; Lehuen A. 2002. NKT cells inhibit the onset of diabetes by impairing the development of pathogenic T cells specific for pancreatic beta cells. Immunity 17(6):725-36. [PubMed: 12479819] [MGI Ref ID J:132259]
Bour-Jordan H; Salomon BL; Thompson HL; Szot GL; Bernhard MR; Bluestone JA. 2004. Costimulation controls diabetes by altering the balance of pathogenic and regulatory T cells. J Clin Invest 114(7):979-87. [PubMed: 15467837] [MGI Ref ID J:93421]
Cain JA; Smith JA; Ondr JK; Wang B; Katz JD. 2006. NKT cells and IFN-gamma establish the regulatory environment for the control of diabetogenic T cells in the nonobese diabetic mouse. J Immunol 176(3):1645-54. [PubMed: 16424194] [MGI Ref ID J:126603]
Calderon B; Suri A; Unanue ER. 2006. In CD4+ T-cell-induced diabetes, macrophages are the final effector cells that mediate islet beta-cell killing: studies from an acute model. Am J Pathol 169(6):2137-47. [PubMed: 17148676] [MGI Ref ID J:116218]
Chen Z; Herman AE; Matos M; Mathis D; Benoist C. 2005. Where CD4+CD25+ T reg cells impinge on autoimmune diabetes. J Exp Med 202(10):1387-97. [PubMed: 16301745] [MGI Ref ID J:118845]
Dai YD; Jensen KP; Lehuen A; Masteller EL; Bluestone JA; Wilson DB; Sercarz EE. 2005. A peptide of glutamic acid decarboxylase 65 can recruit and expand a diabetogenic T cell clone, BDC2.5, in the pancreas. J Immunol 175(6):3621-7. [PubMed: 16148106] [MGI Ref ID J:116720]
Darwiche R; Chong MM; Santamaria P; Thomas HE; Kay TW. 2003. Fas is detectable on beta cells in accelerated, but not spontaneous, diabetes in nonobese diabetic mice. J Immunol 170(12):6292-7. [PubMed: 12794162] [MGI Ref ID J:108698]
Falcone M; Yeung B; Tucker L; Rodriguez E; Krahl T; Sarvetnick N. 2001. IL-4 triggers autoimmune diabetes by increasing self-antigen presentation within the pancreatic Islets. Clin Immunol 98(2):190-9. [PubMed: 11161975] [MGI Ref ID J:127661]
Feuerer M; Jiang W; Holler PD; Satpathy A; Campbell C; Bogue M; Mathis D; Benoist C. 2007. Enhanced thymic selection of FoxP3+ regulatory T cells in the NOD mouse model of autoimmune diabetes. Proc Natl Acad Sci U S A 104(46):18181-6. [PubMed: 17991775] [MGI Ref ID J:127306]
Fife BT; Griffin MD; Abbas AK; Locksley RM; Bluestone JA. 2006. Inhibition of T cell activation and autoimmune diabetes using a B cell surface-linked CTLA-4 agonist. J Clin Invest 116(8):2252-61. [PubMed: 16886063] [MGI Ref ID J:113109]
Friedline RH; Wong CP; Steeber DA; Tedder TF; Tisch R. 2002. L-selectin is not required for T cell-mediated autoimmune diabetes. J Immunol 168(6):2659-66. [PubMed: 11884430] [MGI Ref ID J:126855]
Gonzalez A; Andre-Schmutz I; Carnaud C; Mathis D; Benoist C. 2001. Damage control, rather than unresponsiveness, effected by protective DX5+ T cells in autoimmune diabetes. Nat Immunol 2(12):1117-25. [PubMed: 11713466] [MGI Ref ID J:109860]
Gonzalez A; Katz JD; Mattei MG; Kikutani H; Benoist C; Mathis D. 1997. Genetic control of diabetes progression. Immunity 7(6):873-83. [PubMed: 9430232] [MGI Ref ID J:110546]
Guleria I; Gubbels Bupp M; Dada S; Fife B; Tang Q; Ansari MJ; Trikudanathan S; Vadivel N; Fiorina P; Yagita H; Azuma M; Atkinson M; Bluestone JA; Sayegh MH. 2007. Mechanisms of PDL1-mediated regulation of autoimmune diabetes. Clin Immunol 125(1):16-25. [PubMed: 17627890] [MGI Ref ID J:125272]
Hill NJ; Stotland AB; Sarvetnick NE. 2007. Distinct regulation of autoreactive CD4 T cell expansion by interleukin-4 under conditions of lymphopenia. J Leukoc Biol 81(3):757-65. [PubMed: 17164429] [MGI Ref ID J:118599]
Hoglund P; Mintern J; Waltzinger C; Heath W; Benoist C; Mathis D. 1999. Initiation of autoimmune diabetes by developmentally regulated presentation of islet cell antigens in the pancreatic lymph nodes. J Exp Med 189(2):331-9. [PubMed: 9892615] [MGI Ref ID J:52940]
Holler PD; Yamagata T; Jiang W; Feuerer M; Benoist C; Mathis D. 2007. The same genomic region conditions clonal deletion and clonal deviation to the CD8alphaalpha and regulatory T cell lineages in NOD versus C57BL/6 mice. Proc Natl Acad Sci U S A 104(17):7187-92. [PubMed: 17438291] [MGI Ref ID J:122481]
Horwitz MS; Ilic A; Fine C; Balasa B; Sarvetnick N. 2004. Coxsackieviral-mediated diabetes: induction requires antigen-presenting cells and is accompanied by phagocytosis of beta cells. Clin Immunol 110(2):134-44. [PubMed: 15003810] [MGI Ref ID J:88777]
Judkowski V; Krakowski M; Rodriguez E; Mocnick L; Santamaria P; Sarvetnick N. 2004. Increased islet antigen presentation leads to type-1 diabetes in mice with autoimmune susceptibility. Eur J Immunol 34(4):1031-40. [PubMed: 15048713] [MGI Ref ID J:88883]
Judkowski V; Pinilla C; Schroder K; Tucker L; Sarvetnick N; Wilson DB. 2001. Identification of MHC class II-restricted peptide ligands, including a glutamic acid decarboxylase 65 sequence, that stimulate diabetogenic T cells from transgenic BDC2.5 nonobese diabetic mice. J Immunol 166(2):908-17. [PubMed: 11145667] [MGI Ref ID J:66844]
Kanagawa O; Militech A; Vaupel BA. 2002. Regulation of diabetes development by regulatory T cells in pancreatic islet antigen-specific TCR transgenic nonobese diabetic mice. J Immunol 168(12):6159-64. [PubMed: 12055228] [MGI Ref ID J:89793]
Kanagawa O; Vaupel BA; Xu G; Unanue ER; Katz JD. 1998. Thymic positive selection and peripheral activation of islet antigen-specific T cells: separation of two diabetogenic steps by an I-A(g7) class II MHC beta-chain mutant. J Immunol 161(9):4489-92. [PubMed: 9794372] [MGI Ref ID J:115237]
Keir ME; Liang SC; Guleria I; Latchman YE; Qipo A; Albacker LA; Koulmanda M; Freeman GJ; Sayegh MH; Sharpe AH. 2006. Tissue expression of PD-L1 mediates peripheral T cell tolerance. J Exp Med 203(4):883-95. [PubMed: 16606670] [MGI Ref ID J:123785]
Kim HS; Han MS; Chung KW; Kim S; Kim E; Kim MJ; Jang E; Lee HA; Youn J; Akira S; Lee MS. 2007. Toll-like Receptor 2 Senses beta-Cell Death and Contributes to the Initiation of Autoimmune Diabetes. Immunity 27(2):321-33. [PubMed: 17707128] [MGI Ref ID J:124334]
Kupfer TM; Crawford ML; Pham K; Gill RG. 2005. MHC-mismatched islet allografts are vulnerable to autoimmune recognition in vivo. J Immunol 175(4):2309-16. [PubMed: 16081800] [MGI Ref ID J:107508]
Luhder F; Chambers C; Allison JP; Benoist C; Mathis D. 2000. Pinpointing when T cell costimulatory receptor CTLA-4 must be engaged to dampen diabetogenic T cells. Proc Natl Acad Sci U S A 97(22):12204-9. [PubMed: 11035773] [MGI Ref ID J:109887]
Luhder F; Katz J; Benoist C; Mathis D. 1998. Major histocompatibility complex class II molecules can protect from diabetes by positively selecting T cells with additional specificities. J Exp Med 187(3):379-87. [PubMed: 9449718] [MGI Ref ID J:108722]
Luo X; Tarbell KV; Yang H; Pothoven K; Bailey SL; Ding R; Steinman RM; Suthanthiran M. 2007. Dendritic cells with TGF-beta1 differentiate naive CD4+CD25- T cells into islet-protective Foxp3+ regulatory T cells. Proc Natl Acad Sci U S A 104(8):2821-6. [PubMed: 17307871] [MGI Ref ID J:125908]
Maehr R; Mintern JD; Herman AE; Lennon-Dumenil AM; Mathis D; Benoist C; Ploegh HL. 2005. Cathepsin L is essential for onset of autoimmune diabetes in NOD mice. J Clin Invest 115(10):2934-43. [PubMed: 16184198] [MGI Ref ID J:101527]
Martin-Orozco N; Chen Z; Poirot L; Hyatt E; Chen A; Kanagawa O; Sharpe A; Mathis D; Benoist C. 2003. Paradoxical dampening of anti-islet self-reactivity but promotion of diabetes by OX40 ligand. J Immunol 171(12):6954-60. [PubMed: 14662903] [MGI Ref ID J:86926]
Perone MJ; Bertera S; Tawadrous ZS; Shufesky WJ; Piganelli JD; Baum LG; Trucco M; Morelli AE. 2006. Dendritic cells expressing transgenic galectin-1 delay onset of autoimmune diabetes in mice. J Immunol 177(8):5278-89. [PubMed: 17015713] [MGI Ref ID J:139444]
Phillips JM; Parish NM; Drage M; Cooke A. 2001. Cutting edge: interactions through the IL-10 receptor regulate autoimmune diabetes. J Immunol 167(11):6087-91. [PubMed: 11714766] [MGI Ref ID J:119045]
Raine T; Zaccone P; Mastroeni P; Cooke A. 2006. Salmonella typhimurium infection in nonobese diabetic mice generates immunomodulatory dendritic cells able to prevent type 1 diabetes. J Immunol 177(4):2224-33. [PubMed: 16887982] [MGI Ref ID J:138394]
Rosmalen JG; Martin T; Dobbs C; Voerman JS; Drexhage HA; Haskins K; Leenen PJ. 2000. Subsets of macrophages and dendritic cells in nonobese diabetic mouse pancreatic inflammatory infiltrates: correlation with the development of diabetes. Lab Invest 80(1):23-30. [PubMed: 10652999] [MGI Ref ID J:59988]
Saxena V; Ondr JK; Magnusen AF; Munn DH; Katz JD. 2007. The countervailing actions of myeloid and plasmacytoid dendritic cells control autoimmune diabetes in the nonobese diabetic mouse. J Immunol 179(8):5041-53. [PubMed: 17911589] [MGI Ref ID J:137009]
Shi FD; Flodstrom M; Balasa B; Kim SH; Van Gunst K; Strominger JL; Wilson SB; Sarvetnick N. 2001. Germ line deletion of the CD1 locus exacerbates diabetes in the NOD mouse. Proc Natl Acad Sci U S A 98(12):6777-82. [PubMed: 11390999] [MGI Ref ID J:69908]
Tarbell KV; Petit L; Zuo X; Toy P; Luo X; Mqadmi A; Yang H; Suthanthiran M; Mojsov S; Steinman RM. 2007. Dendritic cell-expanded, islet-specific CD4+ CD25+ CD62L+ regulatory T cells restore normoglycemia in diabetic NOD mice. J Exp Med 204(1):191-201. [PubMed: 17210729] [MGI Ref ID J:125329]
Thomas HE; Irawaty W; Darwiche R; Brodnicki TC; Santamaria P; Allison J; Kay TW. 2004. IL-1 Receptor Deficiency Slows Progression to Diabetes in the NOD Mouse. Diabetes 53(1):113-121. [PubMed: 14693705] [MGI Ref ID J:87251]
Tritt M; Sgouroudis E; d'Hennezel E; Albanese A; Piccirillo CA. 2008. Functional waning of naturally occurring CD4+ regulatory T-cells contributes to the onset of autoimmune diabetes. Diabetes 57(1):113-23. [PubMed: 17928397] [MGI Ref ID J:132415]
Tsui H; Chan Y; Tang L; Winer S; Cheung RK; Paltser G; Selvanantham T; Elford AR; Ellis JR; Becker DJ; Ohashi PS; Dosch HM. 2008. Targeting of pancreatic glia in type 1 diabetes. Diabetes 57(4):918-28. [PubMed: 18198358] [MGI Ref ID J:135214]
Turley SJ; Lee JW; Dutton-Swain N; Mathis D; Benoist C. 2005. Endocrine self and gut non-self intersect in the pancreatic lymph nodes. Proc Natl Acad Sci U S A 102(49):17729-33. [PubMed: 16317068] [MGI Ref ID J:104385]
Ueno A; Cho S; Cheng L; Wang J; Hou S; Nakano H; Santamaria P; Yang Y. 2007. Transient upregulation of indoleamine 2,3-dioxygenase in dendritic cells by human chorionic gonadotropin downregulates autoimmune diabetes. Diabetes 56(6):1686-93. [PubMed: 17360980] [MGI Ref ID J:126514]
Vence L; Benoist C; Mathis D. 2004. Fas deficiency prevents type 1 diabetes by inducing hyporesponsiveness in islet beta-cell-reactive T-cells. Diabetes 53(11):2797-803. [PubMed: 15504959] [MGI Ref ID J:108733]
Wagner DH Jr; Vaitaitis G; Sanderson R; Poulin M; Dobbs C; Haskins K. 2002. Expression of CD40 identifies a unique pathogenic T cell population in type 1 diabetes. Proc Natl Acad Sci U S A 99(6):3782-7. [PubMed: 11891296] [MGI Ref ID J:126524]
Waldner H; Sobel RA; Price N; Kuchroo VK. 2006. The autoimmune diabetes locus Idd9 regulates development of type 1 diabetes by affecting the homing of islet-specific T cells. J Immunol 176(9):5455-62. [PubMed: 16622013] [MGI Ref ID J:131655]
Wallet MA; Sen P; Flores RR; Wang Y; Yi Z; Huang Y; Mathews CE; Earp HS; Matsushima G; Wang B; Tisch R. 2008. MerTK is required for apoptotic cell-induced T cell tolerance. J Exp Med 205(1):219-32. [PubMed: 18195070] [MGI Ref ID J:131291]
Wang B; Gonzalez A; Hoglund P; Katz JD; Benoist C; Mathis D. 1998. Interleukin-4 deficiency does not exacerbate disease in NOD mice. Diabetes 47(8):1207-11. [PubMed: 9703318] [MGI Ref ID J:85924]
Wang J; Cho S; Ueno A; Cheng L; Xu BY; Desrosiers MD; Shi Y; Yang Y. 2008. Ligand-dependent induction of noninflammatory dendritic cells by anergic invariant NKT cells minimizes autoimmune inflammation. J Immunol 181(4):2438-45. [PubMed: 18684934] [MGI Ref ID J:140188]
Wen L; Wong FS; Sherwin R; Mora C. 2002. Human DQ8 can substitute for murine I-A(g7) in the selection of diabetogenic T cells restricted to I-A(g71). J Immunol 168(7):3635-40. [PubMed: 11907129] [MGI Ref ID J:75571]
Yadav D; Judkowski V; Flodstrom-Tullberg M; Sterling L; Redmond WL; Sherman L; Sarvetnick N. 2004. B7-2 (CD86) controls the priming of autoreactive CD4 T cell response against pancreatic islets. J Immunol 173(6):3631-9. [PubMed: 15356107] [MGI Ref ID J:92756]
Zou L; Mendez F; Martin-Orozco N; Peterson EJ. 2008. Defective positive selection results in T cell lymphopenia and increased autoimmune diabetes in ADAP-deficient BDC2.5-C57BL/6 mice. Eur J Immunol 38(4):986-94. [PubMed: 18383041] [MGI Ref ID J:133782]
Andre-Schmutz I; Hindelang C; Benoist C; Mathis D. 1999. Cellular and molecular changes accompanying the progression from insulitis to diabetes. Eur J Immunol 29(1):245-55. [PubMed: 9933106] [MGI Ref ID J:52915]
Balasa B; La Cava A; Van Gunst K; Mocnik L; Balakrishna D; Nguyen N; Tucker L; Sarvetnick N. 2000. A mechanism for IL-10-mediated diabetes in the nonobese diabetic (NOD) mouse: ICAM-1 deficiency blocks accelerated diabetes J Immunol 165(12):7330-7. [PubMed: 11120869] [MGI Ref ID J:66103]
Beaudoin L; Laloux V; Novak J; Lucas B; Lehuen A. 2002. NKT cells inhibit the onset of diabetes by impairing the development of pathogenic T cells specific for pancreatic beta cells. Immunity 17(6):725-36. [PubMed: 12479819] [MGI Ref ID J:132259]
Bour-Jordan H; Salomon BL; Thompson HL; Szot GL; Bernhard MR; Bluestone JA. 2004. Costimulation controls diabetes by altering the balance of pathogenic and regulatory T cells. J Clin Invest 114(7):979-87. [PubMed: 15467837] [MGI Ref ID J:93421]
Cain JA; Smith JA; Ondr JK; Wang B; Katz JD. 2006. NKT cells and IFN-gamma establish the regulatory environment for the control of diabetogenic T cells in the nonobese diabetic mouse. J Immunol 176(3):1645-54. [PubMed: 16424194] [MGI Ref ID J:126603]
Calderon B; Suri A; Unanue ER. 2006. In CD4+ T-cell-induced diabetes, macrophages are the final effector cells that mediate islet beta-cell killing: studies from an acute model. Am J Pathol 169(6):2137-47. [PubMed: 17148676] [MGI Ref ID J:116218]
Chen Z; Herman AE; Matos M; Mathis D; Benoist C. 2005. Where CD4+CD25+ T reg cells impinge on autoimmune diabetes. J Exp Med 202(10):1387-97. [PubMed: 16301745] [MGI Ref ID J:118845]
Dai YD; Jensen KP; Lehuen A; Masteller EL; Bluestone JA; Wilson DB; Sercarz EE. 2005. A peptide of glutamic acid decarboxylase 65 can recruit and expand a diabetogenic T cell clone, BDC2.5, in the pancreas. J Immunol 175(6):3621-7. [PubMed: 16148106] [MGI Ref ID J:116720]
Darwiche R; Chong MM; Santamaria P; Thomas HE; Kay TW. 2003. Fas is detectable on beta cells in accelerated, but not spontaneous, diabetes in nonobese diabetic mice. J Immunol 170(12):6292-7. [PubMed: 12794162] [MGI Ref ID J:108698]
Falcone M; Yeung B; Tucker L; Rodriguez E; Krahl T; Sarvetnick N. 2001. IL-4 triggers autoimmune diabetes by increasing self-antigen presentation within the pancreatic Islets. Clin Immunol 98(2):190-9. [PubMed: 11161975] [MGI Ref ID J:127661]
Feuerer M; Jiang W; Holler PD; Satpathy A; Campbell C; Bogue M; Mathis D; Benoist C. 2007. Enhanced thymic selection of FoxP3+ regulatory T cells in the NOD mouse model of autoimmune diabetes. Proc Natl Acad Sci U S A 104(46):18181-6. [PubMed: 17991775] [MGI Ref ID J:127306]
Fife BT; Griffin MD; Abbas AK; Locksley RM; Bluestone JA. 2006. Inhibition of T cell activation and autoimmune diabetes using a B cell surface-linked CTLA-4 agonist. J Clin Invest 116(8):2252-61. [PubMed: 16886063] [MGI Ref ID J:113109]
Friedline RH; Wong CP; Steeber DA; Tedder TF; Tisch R. 2002. L-selectin is not required for T cell-mediated autoimmune diabetes. J Immunol 168(6):2659-66. [PubMed: 11884430] [MGI Ref ID J:126855]
Gonzalez A; Andre-Schmutz I; Carnaud C; Mathis D; Benoist C. 2001. Damage control, rather than unresponsiveness, effected by protective DX5+ T cells in autoimmune diabetes. Nat Immunol 2(12):1117-25. [PubMed: 11713466] [MGI Ref ID J:109860]
Gonzalez A; Katz JD; Mattei MG; Kikutani H; Benoist C; Mathis D. 1997. Genetic control of diabetes progression. Immunity 7(6):873-83. [PubMed: 9430232] [MGI Ref ID J:110546]
Guleria I; Gubbels Bupp M; Dada S; Fife B; Tang Q; Ansari MJ; Trikudanathan S; Vadivel N; Fiorina P; Yagita H; Azuma M; Atkinson M; Bluestone JA; Sayegh MH. 2007. Mechanisms of PDL1-mediated regulation of autoimmune diabetes. Clin Immunol 125(1):16-25. [PubMed: 17627890] [MGI Ref ID J:125272]
Hill NJ; Stotland AB; Sarvetnick NE. 2007. Distinct regulation of autoreactive CD4 T cell expansion by interleukin-4 under conditions of lymphopenia. J Leukoc Biol 81(3):757-65. [PubMed: 17164429] [MGI Ref ID J:118599]
Hoglund P; Mintern J; Waltzinger C; Heath W; Benoist C; Mathis D. 1999. Initiation of autoimmune diabetes by developmentally regulated presentation of islet cell antigens in the pancreatic lymph nodes. J Exp Med 189(2):331-9. [PubMed: 9892615] [MGI Ref ID J:52940]
Holler PD; Yamagata T; Jiang W; Feuerer M; Benoist C; Mathis D. 2007. The same genomic region conditions clonal deletion and clonal deviation to the CD8alphaalpha and regulatory T cell lineages in NOD versus C57BL/6 mice. Proc Natl Acad Sci U S A 104(17):7187-92. [PubMed: 17438291] [MGI Ref ID J:122481]
Horwitz MS; Ilic A; Fine C; Balasa B; Sarvetnick N. 2004. Coxsackieviral-mediated diabetes: induction requires antigen-presenting cells and is accompanied by phagocytosis of beta cells. Clin Immunol 110(2):134-44. [PubMed: 15003810] [MGI Ref ID J:88777]
Hugues S; Mougneau E; Ferlin W; Jeske D; Hofman P; Homann D; Beaudoin L; Schrike C; Von Herrath M; Lehuen A; Glaichenhaus N. 2002. Tolerance to islet antigens and prevention from diabetes induced by limited apoptosis of pancreatic beta cells. Immunity 16(2):169-81. [PubMed: 11869679] [MGI Ref ID J:132052]
Judkowski V; Krakowski M; Rodriguez E; Mocnick L; Santamaria P; Sarvetnick N. 2004. Increased islet antigen presentation leads to type-1 diabetes in mice with autoimmune susceptibility. Eur J Immunol 34(4):1031-40. [PubMed: 15048713] [MGI Ref ID J:88883]
Judkowski V; Pinilla C; Schroder K; Tucker L; Sarvetnick N; Wilson DB. 2001. Identification of MHC class II-restricted peptide ligands, including a glutamic acid decarboxylase 65 sequence, that stimulate diabetogenic T cells from transgenic BDC2.5 nonobese diabetic mice. J Immunol 166(2):908-17. [PubMed: 11145667] [MGI Ref ID J:66844]
Kanagawa O; Militech A; Vaupel BA. 2002. Regulation of diabetes development by regulatory T cells in pancreatic islet antigen-specific TCR transgenic nonobese diabetic mice. J Immunol 168(12):6159-64. [PubMed: 12055228] [MGI Ref ID J:89793]
Kanagawa O; Vaupel BA; Xu G; Unanue ER; Katz JD. 1998. Thymic positive selection and peripheral activation of islet antigen-specific T cells: separation of two diabetogenic steps by an I-A(g7) class II MHC beta-chain mutant. J Immunol 161(9):4489-92. [PubMed: 9794372] [MGI Ref ID J:115237]
Keir ME; Liang SC; Guleria I; Latchman YE; Qipo A; Albacker LA; Koulmanda M; Freeman GJ; Sayegh MH; Sharpe AH. 2006. Tissue expression of PD-L1 mediates peripheral T cell tolerance. J Exp Med 203(4):883-95. [PubMed: 16606670] [MGI Ref ID J:123785]
Kim HS; Han MS; Chung KW; Kim S; Kim E; Kim MJ; Jang E; Lee HA; Youn J; Akira S; Lee MS. 2007. Toll-like Receptor 2 Senses beta-Cell Death and Contributes to the Initiation of Autoimmune Diabetes. Immunity 27(2):321-33. [PubMed: 17707128] [MGI Ref ID J:124334]
Kupfer TM; Crawford ML; Pham K; Gill RG. 2005. MHC-mismatched islet allografts are vulnerable to autoimmune recognition in vivo. J Immunol 175(4):2309-16. [PubMed: 16081800] [MGI Ref ID J:107508]
Luhder F; Chambers C; Allison JP; Benoist C; Mathis D. 2000. Pinpointing when T cell costimulatory receptor CTLA-4 must be engaged to dampen diabetogenic T cells. Proc Natl Acad Sci U S A 97(22):12204-9. [PubMed: 11035773] [MGI Ref ID J:109887]
Luhder F; Katz J; Benoist C; Mathis D. 1998. Major histocompatibility complex class II molecules can protect from diabetes by positively selecting T cells with additional specificities. J Exp Med 187(3):379-87. [PubMed: 9449718] [MGI Ref ID J:108722]
Luo X; Tarbell KV; Yang H; Pothoven K; Bailey SL; Ding R; Steinman RM; Suthanthiran M. 2007. Dendritic cells with TGF-beta1 differentiate naive CD4+CD25- T cells into islet-protective Foxp3+ regulatory T cells. Proc Natl Acad Sci U S A 104(8):2821-6. [PubMed: 17307871] [MGI Ref ID J:125908]
Maehr R; Mintern JD; Herman AE; Lennon-Dumenil AM; Mathis D; Benoist C; Ploegh HL. 2005. Cathepsin L is essential for onset of autoimmune diabetes in NOD mice. J Clin Invest 115(10):2934-43. [PubMed: 16184198] [MGI Ref ID J:101527]
Martin-Orozco N; Chen Z; Poirot L; Hyatt E; Chen A; Kanagawa O; Sharpe A; Mathis D; Benoist C. 2003. Paradoxical dampening of anti-islet self-reactivity but promotion of diabetes by OX40 ligand. J Immunol 171(12):6954-60. [PubMed: 14662903] [MGI Ref ID J:86926]
Perone MJ; Bertera S; Tawadrous ZS; Shufesky WJ; Piganelli JD; Baum LG; Trucco M; Morelli AE. 2006. Dendritic cells expressing transgenic galectin-1 delay onset of autoimmune diabetes in mice. J Immunol 177(8):5278-89. [PubMed: 17015713] [MGI Ref ID J:139444]
Phillips JM; Parish NM; Drage M; Cooke A. 2001. Cutting edge: interactions through the IL-10 receptor regulate autoimmune diabetes. J Immunol 167(11):6087-91. [PubMed: 11714766] [MGI Ref ID J:119045]
Raine T; Zaccone P; Mastroeni P; Cooke A. 2006. Salmonella typhimurium infection in nonobese diabetic mice generates immunomodulatory dendritic cells able to prevent type 1 diabetes. J Immunol 177(4):2224-33. [PubMed: 16887982] [MGI Ref ID J:138394]
Rosmalen JG; Martin T; Dobbs C; Voerman JS; Drexhage HA; Haskins K; Leenen PJ. 2000. Subsets of macrophages and dendritic cells in nonobese diabetic mouse pancreatic inflammatory infiltrates: correlation with the development of diabetes. Lab Invest 80(1):23-30. [PubMed: 10652999] [MGI Ref ID J:59988]
Saxena V; Ondr JK; Magnusen AF; Munn DH; Katz JD. 2007. The countervailing actions of myeloid and plasmacytoid dendritic cells control autoimmune diabetes in the nonobese diabetic mouse. J Immunol 179(8):5041-53. [PubMed: 17911589] [MGI Ref ID J:137009]
Shi FD; Flodstrom M; Balasa B; Kim SH; Van Gunst K; Strominger JL; Wilson SB; Sarvetnick N. 2001. Germ line deletion of the CD1 locus exacerbates diabetes in the NOD mouse. Proc Natl Acad Sci U S A 98(12):6777-82. [PubMed: 11390999] [MGI Ref ID J:69908]
Tarbell KV; Petit L; Zuo X; Toy P; Luo X; Mqadmi A; Yang H; Suthanthiran M; Mojsov S; Steinman RM. 2007. Dendritic cell-expanded, islet-specific CD4+ CD25+ CD62L+ regulatory T cells restore normoglycemia in diabetic NOD mice. J Exp Med 204(1):191-201. [PubMed: 17210729] [MGI Ref ID J:125329]
Thomas HE; Irawaty W; Darwiche R; Brodnicki TC; Santamaria P; Allison J; Kay TW. 2004. IL-1 Receptor Deficiency Slows Progression to Diabetes in the NOD Mouse. Diabetes 53(1):113-121. [PubMed: 14693705] [MGI Ref ID J:87251]
Tsui H; Chan Y; Tang L; Winer S; Cheung RK; Paltser G; Selvanantham T; Elford AR; Ellis JR; Becker DJ; Ohashi PS; Dosch HM. 2008. Targeting of pancreatic glia in type 1 diabetes. Diabetes 57(4):918-28. [PubMed: 18198358] [MGI Ref ID J:135214]
Turley SJ; Lee JW; Dutton-Swain N; Mathis D; Benoist C. 2005. Endocrine self and gut non-self intersect in the pancreatic lymph nodes. Proc Natl Acad Sci U S A 102(49):17729-33. [PubMed: 16317068] [MGI Ref ID J:104385]
Ueno A; Cho S; Cheng L; Wang J; Hou S; Nakano H; Santamaria P; Yang Y. 2007. Transient upregulation of indoleamine 2,3-dioxygenase in dendritic cells by human chorionic gonadotropin downregulates autoimmune diabetes. Diabetes 56(6):1686-93. [PubMed: 17360980] [MGI Ref ID J:126514]
Vence L; Benoist C; Mathis D. 2004. Fas deficiency prevents type 1 diabetes by inducing hyporesponsiveness in islet beta-cell-reactive T-cells. Diabetes 53(11):2797-803. [PubMed: 15504959] [MGI Ref ID J:108733]
Wagner DH Jr; Vaitaitis G; Sanderson R; Poulin M; Dobbs C; Haskins K. 2002. Expression of CD40 identifies a unique pathogenic T cell population in type 1 diabetes. Proc Natl Acad Sci U S A 99(6):3782-7. [PubMed: 11891296] [MGI Ref ID J:126524]
Waldner H; Sobel RA; Price N; Kuchroo VK. 2006. The autoimmune diabetes locus Idd9 regulates development of type 1 diabetes by affecting the homing of islet-specific T cells. J Immunol 176(9):5455-62. [PubMed: 16622013] [MGI Ref ID J:131655]
Wallet MA; Sen P; Flores RR; Wang Y; Yi Z; Huang Y; Mathews CE; Earp HS; Matsushima G; Wang B; Tisch R. 2008. MerTK is required for apoptotic cell-induced T cell tolerance. J Exp Med 205(1):219-32. [PubMed: 18195070] [MGI Ref ID J:131291]
Wang B; Gonzalez A; Hoglund P; Katz JD; Benoist C; Mathis D. 1998. Interleukin-4 deficiency does not exacerbate disease in NOD mice. Diabetes 47(8):1207-11. [PubMed: 9703318] [MGI Ref ID J:85924]
Wang J; Cho S; Ueno A; Cheng L; Xu BY; Desrosiers MD; Shi Y; Yang Y. 2008. Ligand-dependent induction of noninflammatory dendritic cells by anergic invariant NKT cells minimizes autoimmune inflammation. J Immunol 181(4):2438-45. [PubMed: 18684934] [MGI Ref ID J:140188]
Wen L; Wong FS; Sherwin R; Mora C. 2002. Human DQ8 can substitute for murine I-A(g7) in the selection of diabetogenic T cells restricted to I-A(g71). J Immunol 168(7):3635-40. [PubMed: 11907129] [MGI Ref ID J:75571]
Yadav D; Judkowski V; Flodstrom-Tullberg M; Sterling L; Redmond WL; Sherman L; Sarvetnick N. 2004. B7-2 (CD86) controls the priming of autoreactive CD4 T cell response against pancreatic islets. J Immunol 173(6):3631-9. [PubMed: 15356107] [MGI Ref ID J:92756]
Zou L; Mendez F; Martin-Orozco N; Peterson EJ. 2008. Defective positive selection results in T cell lymphopenia and increased autoimmune diabetes in ADAP-deficient BDC2.5-C57BL/6 mice. Eur J Immunol 38(4):986-94. [PubMed: 18383041] [MGI Ref ID J:133782]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Mating System Hemizygote x +/+ sibling (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.60 Female or Male Hemizygous for Tg(TcraBDC2.5)1Doi, Hemizygous for Tg(TcrbBDC2.5)2Doi *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $207.85 Hemizygous for Tg(TcraBDC2.5)1Doi, Hemizygous for Tg(TcrbBDC2.5)2Doi x Noncarrier, Noncarrier $207.85 Noncarrier, Noncarrier x Hemizygous for Tg(TcraBDC2.5)1Doi, Hemizygous for Tg(TcrbBDC2.5)2Doi
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.30 Female or Male Hemizygous for Tg(TcraBDC2.5)1Doi, Hemizygous for Tg(TcrbBDC2.5)2Doi *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $270.30 Hemizygous for Tg(TcraBDC2.5)1Doi, Hemizygous for Tg(TcrbBDC2.5)2Doi x Noncarrier, Noncarrier $270.30 Noncarrier, Noncarrier x Hemizygous for Tg(TcraBDC2.5)1Doi, Hemizygous for Tg(TcrbBDC2.5)2Doi
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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