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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names BF2(+/-lacZ) (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N?pN1p (25-APR-04)
Generation DefinitionsDonating Investigator Eseng Lai, Merck Development
A targeting vector containing lacZ, neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt most of the coding sequence downstream of the transcription initiation site. The construct was electroporated into 129S1/Sv-p+ Tyr+ KitlSl-J/+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (245 strains)
012463 B6;129S4-Foxd1tm1(GFP/cre)Amc/J 012464 B6;129S4-Foxd1tm2(GFP/cre/ERT2)Amc/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Foxd1tm1Lai/Foxd1tm1Lai
either: (involves: 129S1/Sv) or (involves: 129S1/Sv * C57BL/6J)
- mortality/aging
- complete neonatal lethality
- homozygotes survive to term but die within 24 hours after birth (MGI Ref ID J:33767)
- behavior/neurological phenotype
- aphagia
- although initially indistinguishable from wild-type mice, homozygotes fail to feed (MGI Ref ID J:33767)
- respiratory system phenotype
- tachypnea
- several hours after birth, homozygotes develop a fast respiratory rate (MGI Ref ID J:33767)
- renal/urinary system phenotype
- *normal* renal/urinary system phenotype
- induction and condensation of the nephrogenic mesenchyme occurs normally molecular marker analysis indicated that induction and condensation of the nephrogenic mesenchyme occurs normally (MGI Ref ID J:33767)
- in contrast to the observed abnormalities in nephrogenesis and the morphogenesis of the renal collecting system, the stromal cell component appears morphologically normal the stromal cell component appears morphologically normal (MGI Ref ID J:33767)
- abnormal kidney development
- at E14.5, mutant kidneys have no distinct nephrogenic zone and contain very few S-shaped and comma-shaped bodies, unlike wild-type kidneys (MGI Ref ID J:33767)
- abnormally large mesenchymal aggregations (5-20 cell layers thick) are observed in the cortical and the medullary regions (MGI Ref ID J:33767)
- abnormal metanephric mesenchyme morphology
- at E14.5, differentiation of the metanephric mesenchyme to the tubular structures of the nephrons is inhibited (MGI Ref ID J:33767)
- abnormal nephrogenic mesenchyme morphogenesis
- defects in nephrogenic mesenchyme are observed (MGI Ref ID J:33767)
- abnormal nephrogenic zone morphology
- no distinct nephrogenic zone is observed at E14.5 (MGI Ref ID J:33767)
- delayed kidney development
- mutant kidneys are smaller than wild-type by 2-fold at E14.5, 2.5-fold at E16.5, and 3.3-fold at term (P0) (MGI Ref ID J:33767)
- abnormal nephron morphology
- at term, reduced nephron number is associated with large mesenchymal condensations in the cortical and medullary regions of the kidney (MGI Ref ID J:33767)
- decreased renal glomerulus number
- at term, the total number of glomeruli in mutant kidneys is only about 7% of that in wild-type mice (MGI Ref ID J:33767)
- abnormal ureter morphology
- at E14.5, mutant ureters are located on the ventral side of kidneys (MGI Ref ID J:33767)
- short ureter
- mutant ureters are about one-half of wild-type length but of normal diameter (MGI Ref ID J:33767)
- dilated kidney collecting duct
- at term, the tips of mutant collecting ducts are larger than those in wild-type mice (MGI Ref ID J:33767)
- fused kidneys
- at term, mutant kidneys are fused longitudinally through apposition of the capsule (MGI Ref ID J:33767)
- impaired branching involved in ureteric bud morphogenesis
- pelvic kidney
- at term, mutant kidneys are rotated 90 degrees ventrally, remain in the pelvis rather than ascending to their usual position in the lumbar region, and are thus separated from the adrenal glands (MGI Ref ID J:33767)
- small kidney
- endocrine/exocrine gland phenotype
- abnormal adrenal gland morphology
- mutant adrenal glands are separated from the kidneys (MGI Ref ID J:33767)
- small adrenal glands
- mutant adrenal glands are about half of wild-type size (MGI Ref ID J:33767)
- vision/eye phenotype
- abnormal retina morphology
- mild retinal abnormalities are observed (MGI Ref ID J:33767)
- nervous system phenotype
- abnormal forebrain morphology
- mild forebrain abnormalities are observed (MGI Ref ID J:33767)
- digestive/alimentary phenotype
- meteorism
- several hours after birth, homozygotes accumulate air in their stomach (MGI Ref ID J:33767)
- growth/size phenotype
- meteorism
- several hours after birth, homozygotes accumulate air in their stomach (MGI Ref ID J:33767)
This mouse can be used to support research in many areas including:
Foxd1tm1Lai relatedDevelopmental Biology Research
Internal/Organ Defects
kidney
kidney: vasculature
Perinatal Lethality
Homozygous
Internal/Organ Research
Kidney Defects
Research Tools
lacZ Expression
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: kidney specific marker
Developmental Biology Research
Internal/Organ Defects
kidney
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Foxd1tm1Lai | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Eseng Lai | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | BF-2(-); Foxd1lacZ; | ||
| Mutation Made By | Eseng Lai, Merck | ||
| Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| ES Cell Line Name | CJ7 | ||
| ES Cell Line Strain | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| Site of Expression | lacZ reporter is expressed in site which would normally express Foxd1. Staining pattern in developing kidneys matches wildtype Foxd1 pattern. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Molecular Note | Most of the gene coding region was replaced with a PGK-neo-lacZ cassette with the lacZ gene situated downstream of the transcription initiation site. [MGI Ref ID J:33767] | ||
| Gene Symbol and Name | Foxd1, forkhead box D1 | ||
| Chromosome | 13 | ||
| Gene Common Name(s) | AI385632; BF-2; FKHL8; FREAC4; HNF-3/forkhead homolog, brain factor 2; Hfh10; Hfhbf2; expressed sequence AI385632; | ||
References
References provided by MGI
Selected Reference(s)
Hatini V; Huh SO; Herzlinger D; Soares VC; Lai E. 1996. Essential role of stromal mesenchyme in kidney morphogenesis revealed by targeted disruption of Winged Helix transcription factor BF-2. Genes Dev 10(12):1467-78. [PubMed: 8666231] [MGI Ref ID J:33767]
Additional References
Foxd1tm1Lai relatedBlank U; Brown A; Adams DC; Karolak MJ; Oxburgh L. 2009. BMP7 promotes proliferation of nephron progenitor cells via a JNK-dependent mechanism. Development 136(21):3557-66. [PubMed: 19793891] [MGI Ref ID J:153960]
Carreres MI; Escalante A; Murillo B; Chauvin G; Gaspar P; Vegar C; Herrera E. 2011. Transcription factor foxd1 is required for the specification of the temporal retina in mammals. J Neurosci 31(15):5673-81. [PubMed: 21490208] [MGI Ref ID J:170971]
Herrera E; Marcus R; Li S; Williams SE; Erskine L; Lai E; Mason C. 2004. Foxd1 is required for proper formation of the optic chiasm. Development 131(22):5727-39. [PubMed: 15509772] [MGI Ref ID J:94392]
Levinson RS; Batourina E; Choi C; Vorontchikhina M; Kitajewski J; Mendelsohn CL. 2005. Foxd1-dependent signals control cellularity in the renal capsule, a structure required for normal renal development. Development 132(3):529-39. [PubMed: 15634693] [MGI Ref ID J:95358]
Lin L; Peng SL. 2006. Coordination of NF-kappaB and NFAT antagonism by the forkhead transcription factor Foxd1. J Immunol 176(8):4793-803. [PubMed: 16585573] [MGI Ref ID J:131160]
Qiu L; Hyink DP; Gans WH; Amsler K; Wilson PD; Burrow CR. 2004. Midkine promotes selective expansion of the nephrogenic mesenchyme during kidney organogenesis. Organogenesis 1(1):14-21. [PubMed: 19521555] [MGI Ref ID J:177707]
Schmidt-Ott KM; Chen X; Paragas N; Levinson RS; Mendelsohn CL; Barasch J. 2006. c-kit delineates a distinct domain of progenitors in the developing kidney. Dev Biol 299(1):238-49. [PubMed: 16942767] [MGI Ref ID J:114384]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Breeding & Husbandry The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to C57BL/6 for 7 generations. This strain is maintained as a heterozygote.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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