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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names BF2(+/-lacZ) (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N?pN1p (25-APR-04) Donating Investigator Eseng Lai, Merck Development
A targeting vector containing lacZ, neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt most of the coding sequence downstream of the transcription initiation site. The construct was electroporated into 129S1/Sv-p+ Tyr+ KitlSl-J/+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (186 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Foxd1tm1Lai/Foxd1tm1Lai
either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * C57BL/6)
- lethality-prenatal/perinatal
- neonatal lethality (MGI Ref ID J:33767)
- homozygous mutant mice survive to term but die within 24 hours after birth
- behavior/neurological phenotype
- abnormal eating behavior (MGI Ref ID J:33767)
- although initially indistinguishable from wild-type mice, homozygous mutant mice fail to feed
- endocrine/exocrine gland phenotype
- small adrenal glands (MGI Ref ID J:33767)
- the mutant adrenal glands are approximately one-half the wild-type size and separated from the kidneys
- renal/urinary system phenotype
- *normal* renal/urinary system phenotype (MGI Ref ID J:33767)
- the ovaries, testis, and bladder of homozygous mutant mice appear morphologically normal
- molecular marker analysis indicated that induction and condensation of the nephrogenic mesenchyme occurs normally
- in contrast to the observed abnormalities in nephrogenesis and the morphogenesis of the renal collecting system, the stromal cell component appears morphologically normal
- abnormal kidney morphology (MGI Ref ID J:33767)
- the kidneys of homozygous mutant mice are fused longitudinally through apposition of the capsule, and rotated 90 degrees ventrally
- histological analysis revealed a decrease in the number of differentiated nephrons (25% of the wild-type number) and reduced, abnormal branching of the renal collecting system (8- to 16-fold reduction in the number of branches at E14.5)
- the total number of glomeruli in kidneys of homozygous mutant mice is approximately 7% of the wild-type number
- abnormal kidney development (MGI Ref ID J:33767)
- by E14.5, when the developing kidney usually subdivides into two distinct areas (the cortical zone and a nephrogenic zone), homozygous mutant kidneys have no distinct nephrogenic zone and display abnormally large mesenchymal aggregations (5-20 cell layers thick) in the cortical and the medullary regions of the mutant kidney
- these mesenchymal condensations suggest that the differentiation of the metanephric mesenchyme to nephrons is inhibited
- small kidney (MGI Ref ID J:33767)
- at E12.5, the homozygous mutant and wild-type kidneys are identical in size
- at E14.5, the homozygous mutant kidneys are approximately one-half the wild-type size; at term, they are less than one-third the size of wild-type kidneys
- short ureter (MGI Ref ID J:33767)
- in homozygous mutant mice, the ureter is shorter (approximately one-half the wild-type length, but of normal diameter) and located on the ventral side of the kidneys
- respiratory system phenotype
- abnormal respiration (MGI Ref ID J:33767)
- several hours after birth, homozygous mutant mice develop a fast respiratory rate and accumulate air in their stomach
- vision/eye phenotype
- abnormal retina morphology (MGI Ref ID J:33767)
- homozygous mutant mice display mild retinal abnormalities, including abnormal retinotectal projections
- nervous system phenotype
- abnormal forebrain morphology (MGI Ref ID J:33767)
- homozygous mutant mice display mild forebrain abnormalities (no details are provided)
This mouse can be used to support research in many areas including:
Foxd1tm1Lai relatedDevelopmental Biology Research
Internal/Organ Defects
kidney
kidney: vasculature
Perinatal Lethality
Homozygous
Internal/Organ Research
Kidney Defects
Research Tools
lacZ Expression
Genetics Research
Tissue/Cell Markers: kidney specific marker
Developmental Biology Research
Internal/Organ Defects
kidney
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Foxd1tm1Lai | ||
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| Allele Name | targeted mutation 1, Eseng Lai | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | BF-2(-); Foxd1lacZ; | ||
| Mutation Made By | Eseng Lai, Merck | ||
| Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| ES Cell Line Name | CJ7 | ||
| ES Cell Line Strain | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| Site of Expression | lacZ reporter is expressed in site which would normally express Foxd1. Staining pattern in developing kidneys matches wildtype Foxd1 pattern. | ||
| Gene Symbol and Name | Foxd1, forkhead box D1 | ||
| Chromosome | 13 | ||
| Gene Common Name(s) | AI385632; BF-2; FKHL8; FREAC4; HNF-3/forkhead homolog, brain factor 2; Hfh10; Hfhbf2; expressed sequence AI385632; | ||
| Molecular Note | Most of the gene coding region was replaced with a PGK-neo-lacZ cassette with the lacZ gene situated downstream of the transcription initiation site. [MGI Ref ID J:33767] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Hatini V; Huh SO; Herzlinger D; Soares VC; Lai E. 1996. Essential role of stromal mesenchyme in kidney morphogenesis revealed by targeted disruption of Winged Helix transcription factor BF-2. Genes Dev 10(12):1467-78. [PubMed: 8666231] [MGI Ref ID J:33767]
Additional References
Foxd1tm1Lai relatedBlank U; Brown A; Adams DC; Karolak MJ; Oxburgh L. 2009. BMP7 promotes proliferation of nephron progenitor cells via a JNK-dependent mechanism. Development 136(21):3557-66. [PubMed: 19793891] [MGI Ref ID J:153960]
Herrera E; Marcus R; Li S; Williams SE; Erskine L; Lai E; Mason C. 2004. Foxd1 is required for proper formation of the optic chiasm. Development 131(22):5727-39. [PubMed: 15509772] [MGI Ref ID J:94392]
Levinson RS; Batourina E; Choi C; Vorontchikhina M; Kitajewski J; Mendelsohn CL. 2005. Foxd1-dependent signals control cellularity in the renal capsule, a structure required for normal renal development. Development 132(3):529-39. [PubMed: 15634693] [MGI Ref ID J:95358]
Lin L; Peng SL. 2006. Coordination of NF-kappaB and NFAT antagonism by the forkhead transcription factor Foxd1. J Immunol 176(8):4793-803. [PubMed: 16585573] [MGI Ref ID J:131160]
Schmidt-Ott KM; Chen X; Paragas N; Levinson RS; Mendelsohn CL; Barasch J. 2006. c-kit delineates a distinct domain of progenitors in the developing kidney. Dev Biol 299(1):238-49. [PubMed: 16942767] [MGI Ref ID J:114384]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to C57BL/6 for 7 generations. This strain is maintained as a heterozygote.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Payment Terms and Conditions
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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