Description

Strain Information

Former Names NOD.B6-(D6Mit254-D6Mit14)/CarJ    (Changed: 03-JAN-05 ) NOD.Nk1.1    (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain NOD/Necker Donor Strain C57BL/6JOrl Generation N10F?+N4F4p (17-JUL-05)   Donating Investigator Claude Carnaud,   INSERM

Appearance
albino, pink-eyed
Related Genotype: A/A Tyr^c/Tyr^c

Description
Klrb1c^a homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Diabetes incidence as reported by donating investigator and confirmed in the T1DR, prior to shortening the congenic interval, is significantly decreased (30% females and 0% males at 30 weeks of age) as compared to standard NOD (NK1.2) controls (85% females and 60% males at 30 weeks of age) . FACS analysis of NKT cells derived from thymus or spleen of NOD animals homozygous for this genomic segment revealed the presence of NK1.1+/TCR alpha beta +, V beta8+ and CD44+, but at lower levels than in C57BL/6 controls. NK cells of NOD.B6-(D6Mit254-D6Mit14) exhibit an equivalent number of NK1.1/DX5 double positive cells when compared to C57BL/6. Histological examination of 10-12 week old animals indicates no significant difference in insulitis severity between NOD.B6-(D6Mit254-D6Mit14) and NOD control islets (Carnaud et al, 2001). Confirming the published report that insulitis development in NK1.1 congenics is not significantly different from control NOD (NK1.2+)(Carnaud et al, 2001), sampling of non-diabetic NOD.NK1.1 congenic mice in the TIDR at 30 wk of age also showed extensive insulitis. This model is useful for looking at the role of natural killer cells in autoimmune diseases such as Type 1 Diabetes.

Development
A Chromosome 6 genomic segment from C57BL/6JOrl originally reported to span D6Mit254 through D6Mit14 and containing Klrb1c^a (commonly referred to as NK1.1) was transferred through 10 generations of backcrossing to NOD/Necker (Carnaud et al, 2001). In 2002, this strain arrived at TJL at N10F?. Further a selected genome scan ("Idd sweep" and "Chromosome of interest") indicated that some animals of this strain were heterozygous for a C57BL/6-derived marker on Chromosome 3(D3Mit95) as well as carrying a longer Chromosome 6 congenic interval (D6Mit328 - D6Mit15) than originally published. These animals were backcrossed 4 times to NOD/LtJ (JR1976) prior to making the stock homozygous for a smaller C57BL/6JOrl-derived interval (D6Mit254-D6Mit339) around NK1.1 (62.1 cM) and to eliminate heterozygosity at D3Mit95.

Control Information

	Control
	001976 NOD/ShiLtJ
Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM)
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)

Genes & Alleles

Gene & Allele Information

Gene Symbol and Name		D6Mit254, DNA segment, Chr 6, Massachusetts Institute of Technology 254
Chromosome		6
Gene Symbol and Name		D6Mit339, DNA Segment, Chr 6, Massachusetts Institute of Technology 339
Chromosome		6
Allele Symbol		Klrb1ca
Allele Name		antigen Nk-1.1 allele
Allele Type		Not Applicable
Common Name(s)		nk1.1;
Mutation Made By		Claude Carnaud,   INSERM
Gene Symbol and Name		Klrb1c, killer cell lectin-like receptor subfamily B member 1C
Chromosome		6
Gene Common Name(s)		AI462337; CD161; Ly-59; Ly55c; Ly59; NK-1.1; NK-RP1; NKR-P1; NKR-P1C; Nk-1; Nk-1.2; Nk1; Nk1.1; Nkrp1-c; expressed sequence AI462337; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 59; natural killer cell-associated antigen 1;

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Carnaud C; Gombert J; Donnars O; Garchon H; Herbelin A. 2001. Protection against diabetes and improved NK/NKT cell performance in NOD.NK1.1 mice congenic at the NK complex. J Immunol 166(4):2404-11. [PubMed: 11160299]  [MGI Ref ID J:68580]

Additional References

Klrb1c^a related

Burton RC; Winn HJ. 1981. Studies on natural killer (NK) cells. I. NK cell specific antibodies in CE anti-CBA serum. J Immunol 126(5):1985-9. [PubMed: 7217676]  [MGI Ref ID J:6490]

Pollack SB; Emmons SL. 1982. NK-2.1: an NK-associated antigen detected with NZB anti-BALB/c serum. J Immunol 129(5):2277-81. [PubMed: 6749989]  [MGI Ref ID J:6862]

Ryan JC; Turck J; Niemi EC; Yokoyama WM; Seaman WE. 1992. Molecular cloning of the NK1.1 antigen, a member of the NKR-P1 family of natural killer cell activation molecules. J Immunol 149(5):1631-5. [PubMed: 1506685]  [MGI Ref ID J:2388]

Serizawa I; Koezuka Y; Amao H; Saito TR; Takahashi KW. 2000. Functional natural killer T cells in experimental mouse strains, including NK1.1- strains Exp Anim 49(3):171-80. [PubMed: 11109539]  [MGI Ref ID J:64139]

Stenstrom M; Skold M; Andersson A; Cardell SL. 2005. Natural killer T-cell populations in C57BL/6 and NK1.1 congenic BALB.NK mice-a novel thymic subset defined in BALB.NK mice. Immunology 114(3):336-45. [PubMed: 15720435]  [MGI Ref ID J:97461]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Type 1 Diabetes Repository collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	001976 NOD/ShiLtJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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General Terms and Conditions

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