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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names NOD.B6-(D6Mit254-D6Mit14)/CarJ (Changed: 03-JAN-05 ) NOD.Nk1.1 (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain NOD/Necker Donor Strain C57BL/6JOrl Generation N10F?+N4F4p (17-JUL-05)
Generation DefinitionsDonating Investigator Claude Carnaud, INSERM Appearance
albino, pink-eyed
Related Genotype: A/A Tyrc/TyrcDescription
Klrb1ca homozygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Diabetes incidence as reported by donating investigator and confirmed in the T1DR, prior to shortening the congenic interval, is significantly decreased (30% females and 0% males at 30 weeks of age) as compared to standard NOD (NK1.2) controls (85% females and 60% males at 30 weeks of age) . FACS analysis of NKT cells derived from thymus or spleen of NOD animals homozygous for this genomic segment revealed the presence of NK1.1+/TCR alpha beta +, V beta8+ and CD44+, but at lower levels than in C57BL/6 controls. NK cells of NOD.B6-(D6Mit254-D6Mit14) exhibit an equivalent number of NK1.1/DX5 double positive cells when compared to C57BL/6. Histological examination of 10-12 week old animals indicates no significant difference in insulitis severity between NOD.B6-(D6Mit254-D6Mit14) and NOD control islets (Carnaud et al, 2001). Confirming the published report that insulitis development in NK1.1 congenics is not significantly different from control NOD (NK1.2+)(Carnaud et al, 2001), sampling of non-diabetic NOD.NK1.1 congenic mice in the TIDR at 30 wk of age also showed extensive insulitis. This model is useful for looking at the role of natural killer cells in autoimmune diseases such as Type 1 Diabetes.Development
A Chromosome 6 genomic segment from C57BL/6JOrl originally reported to span D6Mit254 through D6Mit14 and containing Klrb1ca (commonly referred to as NK1.1) was transferred through 10 generations of backcrossing to NOD/Necker (Carnaud et al, 2001). In 2002, this strain arrived at TJL at N10F?. Further a selected genome scan ("Idd sweep" and "Chromosome of interest") indicated that some animals of this strain were heterozygous for a C57BL/6-derived marker on Chromosome 3(D3Mit95) as well as carrying a longer Chromosome 6 congenic interval (D6Mit328 - D6Mit15) than originally published. These animals were backcrossed 4 times to NOD/LtJ (JR1976) prior to making the stock homozygous for a smaller C57BL/6JOrl-derived interval (D6Mit254-D6Mit339) around NK1.1 (62.1 cM) and to eliminate heterozygosity at D3Mit95.
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of D6Mit254
007573 NOD.B6-(D6Mit254-D6Mit289)/CarJ View Strains carrying other alleles of D6Mit254 (1 strain)
007573 NOD.B6-(D6Mit254-D6Mit289)/CarJ
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM)
Type 1 Diabetes (IDDM) Analysis Strains
NOD/ShiLtJ Non-MHC Congenics
Genes & Alleles
Gene & Allele Information
| Gene Symbol and Name | D6Mit254, DNA segment, Chr 6, Massachusetts Institute of Technology 254 | ||
|---|---|---|---|
| Chromosome | 6 | ||
| Gene Symbol and Name | D6Mit339, DNA Segment, Chr 6, Massachusetts Institute of Technology 339 | ||
| Chromosome | 6 | ||
| Allele Symbol | Klrb1ca | ||
| Allele Name | antigen Nk-1.1 allele | ||
| Allele Type | Not Applicable | ||
| Common Name(s) | nk1.1; | ||
| Mutation Made By | Claude Carnaud, INSERM | ||
| Gene Symbol and Name | Klrb1c, killer cell lectin-like receptor subfamily B member 1C | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AI462337; CD161; Ly-59; Ly55c; Ly59; NK-1.1; NK-RP1; NKR-P1; NKR-P1C; Nk-1; Nk-1.2; Nk1; Nk1.1; Nkrp1-c; expressed sequence AI462337; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 59; natural killer cell-associated antigen 1; | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Carnaud C; Gombert J; Donnars O; Garchon H; Herbelin A. 2001. Protection against diabetes and improved NK/NKT cell performance in NOD.NK1.1 mice congenic at the NK complex. J Immunol 166(4):2404-11. [PubMed: 11160299] [MGI Ref ID J:68580]
Additional References
Klrb1ca relatedBurton RC; Winn HJ. 1981. Studies on natural killer (NK) cells. I. NK cell specific antibodies in CE anti-CBA serum. J Immunol 126(5):1985-9. [PubMed: 7217676] [MGI Ref ID J:6490]
Pollack SB; Emmons SL. 1982. NK-2.1: an NK-associated antigen detected with NZB anti-BALB/c serum. J Immunol 129(5):2277-81. [PubMed: 6749989] [MGI Ref ID J:6862]
Ryan JC; Turck J; Niemi EC; Yokoyama WM; Seaman WE. 1992. Molecular cloning of the NK1.1 antigen, a member of the NKR-P1 family of natural killer cell activation molecules. J Immunol 149(5):1631-5. [PubMed: 1506685] [MGI Ref ID J:2388]
Serizawa I; Koezuka Y; Amao H; Saito TR; Takahashi KW. 2000. Functional natural killer T cells in experimental mouse strains, including NK1.1- strains Exp Anim 49(3):171-80. [PubMed: 11109539] [MGI Ref ID J:64139]
Stenstrom M; Skold M; Andersson A; Cardell SL. 2005. Natural killer T-cell populations in C57BL/6 and NK1.1 congenic BALB.NK mice-a novel thymic subset defined in BALB.NK mice. Immunology 114(3):336-45. [PubMed: 15720435] [MGI Ref ID J:97461]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $1600.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryopreserved Embryos
This strain is also available as cryopreserved embryos. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $2080.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryopreserved Embryos
This strain is also available as cryopreserved embryos. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Type 1 Diabetes Repository collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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