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Description
Strain Information
Former Names B6.FVB-Tg(Itgax-DTR/GFP)57Drl (Changed: 15-DEC-04 ) B6.FVB-Tg(Itgax-DTR/GFP)57Lan/J (Changed: 15-DEC-04 ) B6.FVB-Tg(Itgax-DTR/GFP)57Litt (Changed: 15-DEC-04 ) CD11c-DTR (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation N5+N14 (23-DEC-08) Donating Investigator Dan Littman, New York University Medical Center Description
Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. Upon diphtheria toxin (DT) administration, mice harboring this transgene are transiently depleted of dendritic cell (DC) populations. All CD8+ and CD8- DC in the spleen express EGFP and are DT sensitive. Immunohistochemical and flow cytometric analysis reveals EGFP expression and DT-inducible depletion of CD11chigh DC in spleen, lymph node, lung, liver and lamina propria tissues, as well as the defined macrophage subpopulations of the alveolar, lamina propria, metallophillic and marginal zone. Rapid reduction of CD11c+ DC populations induced by intraperitoneal injection of DT persists for approximately 2 days, after which the cell population gradually recovers. While transient DC depletion was not associated with sign of illness or long-term defects, repeated DT induction is lethal to the mouse. Long term depletion of this cell population can be achieved by the generation of mixed irradiation chimeras. This mutant mouse strain may be useful in studies of mononuclear phagocyte origins and the specific role of dendritic cells in the immune response.Development
A transgene was designed to place a simian Diphtheria Toxin Receptor (DTR)-Enhanced Green Fluorescent Protein (EGFP, Stratagene) fusion protein under the control of the Itgax (or CD11c) promoter. This transgene was introduced into fertilized FVB/N donor eggs. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for 5 generations. This transgene has been mapped to mouse Chromosome 1 (~52 cM; 87395187bp position) via single nucleotide polymorphism (SNP) analysis by The Jackson Laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
004512 C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J 008549 NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ
008068 B6.Cg-Tg(Itgax-cre)1-1Reiz/J 007567 C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J 009422 NOD.Cg-Tg(Itgax-EYFP)1Mnz/QtngJ
006000 B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J 005515 FVB-Tg(ITGAM-DTR/EGFP)34Lan/J 008547 NOD.FVB-Tg(ITGAM-DTR/EGFP)34Lan/JdkJ
Additional Web Information
Fluorescent Proteins/lacZ Systems
JAX® NOTES, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
Phenotype
Phenotype Information
assigned by genotype
Tg(Itgax-DTR/EGFP)57Lan/0
B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
- life span-post-weaning/aging
- increased sensitivity to induced morbidity/mortality (MGI Ref ID J:113232)
- mice exhibit an increase in mortality compared to wild-type mice following diphtheria treatment and cecal ligation puncture (CLP) (100% compared to 45% mortality)
- however, replacement of dendritic cells with wild-type dendritic cells improves survival (mortality drops from 100% to 65%)
- immune system phenotype
- *normal* immune system phenotype (MGI Ref ID J:122114)
- depletion of Cd11c+ dendritic cells by treatment with diphtheria toxin does not affect accumulation and localization of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in T cell zones of the lymph node and spleen
- conventional T cell, macrophages, B cell, NK cell, and NK T cell numbers are unaffected by treatment with diphtheria toxin
- despite an increase in mortality following diphtheria treatment and cecal ligation puncture, mice do not exhibit an increase in bacteremia or cytokine production
- abnormal NK T cell physiology (MGI Ref ID J:96123)
- following treatment with diphtheria toxin and stimulation with alpha-GalCer, 5% of spleen NK T cells stain positive for IFN-gamma and 8% for IL-4 compared to 32% and 30%, respectively, of similarly treated wild-type NK T cells
- however, normal levels of IFN-gamma and IL-4 production where observed in liver NK T cells
- abnormal NK cell physiology (MGI Ref ID J:96123)
- following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin
- following treatment with diphtheria toxin and stimulation with alpha-C-GalCer, less than 1% at 6 and 0.5% to 1% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 6% to 8% and 12% to 14%, respectively, of cells from transgenic mice not treated with diphtheria toxin
- impaired NK cell cytolysis (MGI Ref ID J:125611)
- unlike in wild-type mice, NK T cell activation measured by specific cell lysis following infection with Leishmania infantum in diphtheria toxin treated mice does not occur
- decreased circulating interferon-gamma level (MGI Ref ID J:96123)
- following treatment with diphtheria toxin, little IFN-gamma in alpha-GalCer-injected mice and no IFN-gamma in alpha-C-GalCer-injected mice was detected unlike in transgenic mice not treated with diphtheria toxin
- decreased circulating interleukin-12 level (MGI Ref ID J:96123)
- following treatment with diphtheria toxin and stimulation with alpha-GalCer or alpha-C-GalCer, virtually no serum IL-12 was detected unlike in transgenic mice not treated with diphtheria toxin
- decreased dendritic cell number (MGI Ref ID J:100867)
- CD11c+ (Itgax+) dendritic cells in lung parenchyma are depleted following treatment with diphtheria toxin
- 24 hours after treatment with diphtheria toxin, spleens and livers are depleted of dendritic cells
- treatment with diphtheria toxin depletes CD11chighMHCIIhigh dendritic cells
- decreased interferon-gamma secretion (MGI Ref ID J:96123)
- following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin
- following treatment with diphtheria toxin and stimulation with alpha-GalCer, 5% of spleen NK T cells stain positive for IFN-gamma compared to 32% of similarly treated wild-type NK T cells
- however, normal levels of production where observed in liver NK T cells
- following treatment with diphtheria toxin and stimulation with alpha-C-GalCer, less than 1% at 6 and 0.5% to 1% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 6% to 8% and 12% to 14%, respectively, of cells from transgenic mice not treated with diphtheria toxin
- decreased interleukin-12b secretion (MGI Ref ID J:125611)
- unlike in wild-type mice, IL-12p40 production following infection with Leishmania infantum in diphtheria toxin treated mice does not occur
- decreased interleukin-4 secretion (MGI Ref ID J:96123)
- following treatment with diphtheria toxin and stimulation with alpha-GalCer, 8% of spleen NK T cells stain positive for IL-4 compared to 30% of similarly treated wild-type NK T cells however, normal levels of production where observed in liver NK T cells
- sepsis (MGI Ref ID J:113232)
- mice exhibit an increase in mortality compared to wild-type mice following diphtheria treatment and cecal ligation puncture (CLP) (100% compared to 45% mortality)
- homeostasis/metabolism phenotype
- decreased circulating interferon-gamma level (MGI Ref ID J:96123)
- following treatment with diphtheria toxin, little IFN-gamma in alpha-GalCer-injected mice and no IFN-gamma in alpha-C-GalCer-injected mice was detected unlike in transgenic mice not treated with diphtheria toxin
- decreased circulating interleukin-12 level (MGI Ref ID J:96123)
- following treatment with diphtheria toxin and stimulation with alpha-GalCer or alpha-C-GalCer, virtually no serum IL-12 was detected unlike in transgenic mice not treated with diphtheria toxin
- hematopoietic system phenotype
- decreased dendritic cell number (MGI Ref ID J:100867)
- CD11c+ (Itgax+) dendritic cells in lung parenchyma are depleted following treatment with diphtheria toxin
- 24 hours after treatment with diphtheria toxin, spleens and livers are depleted of dendritic cells
- treatment with diphtheria toxin depletes CD11chighMHCIIhigh dendritic cells
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Itgax-DTR/EGFP)57Lan/0
C.FVB-Tg(Itgax-DTR/EGFP)57Lan
- life span-post-weaning/aging
- premature death (MGI Ref ID J:93488)
- repeated treatment with diphtheria toxin results in lethality due to dendritic cell depletion
- immune system phenotype
- abnormal CD8-positive T cell differentiation (MGI Ref ID J:93488)
- adoptive transfer experiments demonstrate that CD8+ T cell priming with cell-associated antigen does not occur in diphtheria-induced dendritic cell depleted mice
- abnormal T cell activation (MGI Ref ID J:93488)
- treatment with diphtheria toxin results in a loss of in vitro stimulation of alloreactive T cells
- decreased CD8-positive T cell number (MGI Ref ID J:93488)
- treatment with diphtheria toxin results in a substantial loss of CD8+ T cells
- decreased dendritic cell number (MGI Ref ID J:93488)
- CD11c+ (Itgax+) dendritic cells are depleted for 2 days following treatment with diphtheria toxin
- defective cytotoxic T cell cytolysis (MGI Ref ID J:93488)
- in adoptive transfer experiments using diphtheria-induced dendritic cell depleted mice infected with Listeria or malaria at the liver stage
- hematopoietic system phenotype
- abnormal CD8-positive T cell differentiation (MGI Ref ID J:93488)
- adoptive transfer experiments demonstrate that CD8+ T cell priming with cell-associated antigen does not occur in diphtheria-induced dendritic cell depleted mice
- abnormal T cell activation (MGI Ref ID J:93488)
- treatment with diphtheria toxin results in a loss of in vitro stimulation of alloreactive T cells
- decreased CD8-positive T cell number (MGI Ref ID J:93488)
- treatment with diphtheria toxin results in a substantial loss of CD8+ T cells
- decreased dendritic cell number (MGI Ref ID J:93488)
- CD11c+ (Itgax+) dendritic cells are depleted for 2 days following treatment with diphtheria toxin
Tg(Itgax-DTR/EGFP)57Lan/0
C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
- immune system phenotype
- *normal* immune system phenotype (MGI Ref ID J:96123)
- conventional T cell, macrophages, B cell, NK cell, and NK T cell numbers are unaffected by treatment with diphtheria toxin
- abnormal NK cell physiology (MGI Ref ID J:96123)
- following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin
- decreased circulating interferon-gamma level (MGI Ref ID J:96123)
- following treatment with diphtheria toxin, little IFN-gamma in alpha-GalCer-injected mice and no IFN-gamma in alpha-C-GalCer-injected mice was detected unlike in transgenic mice not treated with diphtheria toxin
- decreased circulating interleukin-12 level (MGI Ref ID J:96123)
- following treatment with diphtheria toxin and stimulation with alpha-GalCer or alpha-C-GalCer, virtually no serum IL-12 was detected unlike in transgenic mice not treated with diphtheria toxin
- decreased dendritic cell number (MGI Ref ID J:96123)
- 24 hours after treatment with diphtheria toxin, spleens and livers are depleted of dendritic cells
- decreased interferon-gamma secretion (MGI Ref ID J:96123)
- following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin
- homeostasis/metabolism phenotype
- decreased circulating interferon-gamma level (MGI Ref ID J:96123)
- following treatment with diphtheria toxin, little IFN-gamma in alpha-GalCer-injected mice and no IFN-gamma in alpha-C-GalCer-injected mice was detected unlike in transgenic mice not treated with diphtheria toxin
- decreased circulating interleukin-12 level (MGI Ref ID J:96123)
- following treatment with diphtheria toxin and stimulation with alpha-GalCer or alpha-C-GalCer, virtually no serum IL-12 was detected unlike in transgenic mice not treated with diphtheria toxin
- hematopoietic system phenotype
- decreased dendritic cell number (MGI Ref ID J:96123)
- 24 hours after treatment with diphtheria toxin, spleens and livers are depleted of dendritic cells
Tg(Itgax-DTR/EGFP)57Lan/0
involves: C57BL/6 * FVB/N
- immune system phenotype
- abnormal alveolar macrophage morphology (MGI Ref ID J:137452)
- CD11b+ Langerhans cells and alveolar macrophage are depleted following intratracheally administration of diphtheria toxin
- decreased dendritic cell number (MGI Ref ID J:137452)
- CD11b+ Langerhans cells and alveolar macrophage are depleted following intratracheally administration of diphtheria toxin
- mesenteric lymph nodes are partially depleted following intratracheally administration of diphtheria toxin
- decreased interferon-gamma secretion (MGI Ref ID J:137452)
- following administration of diphtheria toxin and CD3 stimulation, IFN-gamma production by CD4 and CD8 T cells from the mesenteric lymph nodes is decreased compared to in similarly treated wild-type mice
- increased susceptibility to viral infection (MGI Ref ID J:137452)
- following intratracheally administration of diphtheria toxin, mice exhibit increased severity of influenza infection, increased weight lose and delayed clearance compared to infected wild-type mice
- the number of virus-specific cytotoxic T lymphoctes is reduced in mice treated with diphtheria toxin and infected with influenza compared to similarly treated wild-type mcie
- respiratory system phenotype
- abnormal alveolar macrophage morphology (MGI Ref ID J:137452)
- CD11b+ Langerhans cells and alveolar macrophage are depleted following intratracheally administration of diphtheria toxin
- hematopoietic system phenotype
- abnormal alveolar macrophage morphology (MGI Ref ID J:137452)
- CD11b+ Langerhans cells and alveolar macrophage are depleted following intratracheally administration of diphtheria toxin
- decreased dendritic cell number (MGI Ref ID J:137452)
- CD11b+ Langerhans cells and alveolar macrophage are depleted following intratracheally administration of diphtheria toxin
- mesenteric lymph nodes are partially depleted following intratracheally administration of diphtheria toxin
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cancer Research
tumor immunology
xenograft/transplant host
Fluorescent Proteins
Immunology and Inflammation Research
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Itgax-DTR/EGFP)57Lan | ||
|---|---|---|---|
| Allele Name | transgene insertion 57, Richard A Lang | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | CD11c-DTR; CD11c-DTR/GFP; CD11c/DTR Tg; CD11cDTR; CD11cDTR tg; DCKO; DTR-GFP; DTR-GRP; Tg(Itgax-DTR/GFP)57Lan; Tg.Itgax-DTR/EGFP.57Lan; TgItgax-DTR/EGFP.57Lan; | ||
| Mutation Made By | Steffen Jung, Weizmann Institute of Science | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Expression of the transgene is specific to splenic dendritic cells. All CD8+ and CD8- dentritic cells in the spleen fluoresce and are DT sensititve. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | DTR, Simian Diphtheria Toxin Receptor, | ||
| Promoter | Itgax, integrin alpha X, mouse, laboratory | ||
| Molecular Note | The transgene contains sequence encoding DTR/GFP under the control of the mouse Itgax promoter. Two lines were creacted (line 11 and line 57) with 1 to 2 copies and 20 copies of the transgene, respectively. All CD8+ and CD8- dendritic cells in the spleen fluoresce and are DT sensitive. Immunohistochemical and flow cytometric analyses show that expression of the transgene is specific to splenic dendritic cells. [MGI Ref ID J:93488] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Fluorescent Proteins -- Generic GFP, QPCR
TG(DTR/EGFP), Standard PCR
Tg(ITGAM-DTR/EGFP), Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Jung S; Unutmaz D; Wong P; Sano G; De los Santos K; Sparwasser T; Wu S; Vuthoori S; Ko K; Zavala F; Pamer EG; Littman DR; Lang RA. 2002. In vivo depletion of CD11c(+) dendritic cells abrogates priming of CD8(+) T cells by exogenous cell-associated antigens. Immunity 17(2):211-20. [PubMed: 12196292] [MGI Ref ID J:93488]
Additional References
Tg(Itgax-DTR/EGFP)57Lan relatedBates JT; Uematsu S; Akira S; Mizel SB. 2009. Direct stimulation of tlr5+/+ CD11c+ cells is necessary for the adjuvant activity of flagellin. J Immunol 182(12):7539-47. [PubMed: 19494277] [MGI Ref ID J:149297]
Bessa J; Jegerlehner A; Hinton HJ; Pumpens P; Saudan P; Schneider P; Bachmann MF. 2009. Alveolar macrophages and lung dendritic cells sense RNA and drive mucosal IgA responses. J Immunol 183(6):3788-99. [PubMed: 19710454] [MGI Ref ID J:152307]
Bianchi T; Pincus LB; Wurbel MA; Rich BE; Kupper TS; Fuhlbrigge RC; Boes M. 2009. Maintenance of peripheral tolerance through controlled tissue homing of antigen-specific T cells in K14-mOVA mice. J Immunol 182(8):4665-74. [PubMed: 19342642] [MGI Ref ID J:147741]
Birnberg T; Bar-On L; Sapoznikov A; Caton ML; Cervantes-Barragan L; Makia D; Krauthgamer R; Brenner O; Ludewig B; Brockschnieder D; Riethmacher D; Reizis B; Jung S. 2008. Lack of conventional dendritic cells is compatible with normal development and T cell homeostasis, but causes myeloid proliferative syndrome. Immunity 29(6):986-97. [PubMed: 19062318] [MGI Ref ID J:142682]
Bogunovic M; Ginhoux F; Helft J; Shang L; Hashimoto D; Greter M; Liu K; Jakubzick C; Ingersoll MA; Leboeuf M; Stanley ER; Nussenzweig M; Lira SA; Randolph GJ; Merad M. 2009. Origin of the lamina propria dendritic cell network. Immunity 31(3):513-25. [PubMed: 19733489] [MGI Ref ID J:152688]
Butovsky O; Kunis G; Koronyo-Hamaoui M; Schwartz M. 2007. Selective ablation of bone marrow-derived dendritic cells increases amyloid plaques in a mouse Alzheimer's disease model. Eur J Neurosci 26(2):413-6. [PubMed: 17623022] [MGI Ref ID J:127277]
Ciavarra RP; Stephens A; Nagy S; Sekellick M; Steel C. 2006. Evaluation of immunological paradigms in a virus model: are dendritic cells critical for antiviral immunity and viral clearance? J Immunol 177(1):492-500. [PubMed: 16785546] [MGI Ref ID J:134380]
Darrasse-Jeze G; Deroubaix S; Mouquet H; Victora GD; Eisenreich T; Yao KH; Masilamani RF; Dustin ML; Rudensky A; Liu K; Nussenzweig MC. 2009. Feedback control of regulatory T cell homeostasis by dendritic cells in vivo. J Exp Med 206(9):1853-62. [PubMed: 19667061] [MGI Ref ID J:152171]
Denning TL; Wang YC; Patel SR; Williams IR; Pulendran B. 2007. Lamina propria macrophages and dendritic cells differentially induce regulatory and interleukin 17-producing T cell responses. Nat Immunol 8(10):1086-94. [PubMed: 17873879] [MGI Ref ID J:125258]
Desai DD; Harbers SO; Flores M; Colonna L; Downie MP; Bergtold A; Jung S; Clynes R. 2007. Fc gamma receptor IIB on dendritic cells enforces peripheral tolerance by inhibiting effector T cell responses. J Immunol 178(10):6217-26. [PubMed: 17475849] [MGI Ref ID J:146123]
Didierlaurent A; Goulding J; Patel S; Snelgrove R; Low L; Bebien M; Lawrence T; van Rijt LS; Lambrecht BN; Sirard JC; Hussell T. 2008. Sustained desensitization to bacterial Toll-like receptor ligands after resolution of respiratory influenza infection. J Exp Med 205(2):323-9. [PubMed: 18227219] [MGI Ref ID J:131869]
Engel D; Dobrindt U; Tittel A; Peters P; Maurer J; Gutgemann I; Kaissling B; Kuziel W; Jung S; Kurts C. 2006. Tumor necrosis factor alpha- and inducible nitric oxide synthase-producing dendritic cells are rapidly recruited to the bladder in urinary tract infection but are dispensable for bacterial clearance. Infect Immun 74(11):6100-7. [PubMed: 16966414] [MGI Ref ID J:113552]
Fujii S; Goto A; Shimizu K. 2009. Antigen mRNA-transfected, allogeneic fibroblasts loaded with NKT-cell ligand confer antitumor immunity. Blood 113(18):4262-72. [PubMed: 19164596] [MGI Ref ID J:148431]
Fukuyama T; Kasper LH; Boussouar F; Jeevan T; van Deursen J; Brindle PK. 2009. Histone acetyltransferase CBP is vital to demarcate conventional and innate CD8+ T-cell development. Mol Cell Biol 29(14):3894-904. [PubMed: 19433445] [MGI Ref ID J:150146]
GeurtsvanKessel CH; Willart MA; van Rijt LS; Muskens F; Kool M; Baas C; Thielemans K; Bennett C; Clausen BE; Hoogsteden HC; Osterhaus AD; Rimmelzwaan GF; Lambrecht BN. 2008. Clearance of influenza virus from the lung depends on migratory langerin+CD11b- but not plasmacytoid dendritic cells. J Exp Med 205(7):1621-34. [PubMed: 18591406] [MGI Ref ID J:137452]
Guimond M; Veenstra RG; Grindler DJ; Zhang H; Cui Y; Murphy RD; Kim SY; Na R; Hennighausen L; Kurtulus S; Erman B; Matzinger P; Merchant MS; Mackall CL. 2009. Interleukin 7 signaling in dendritic cells regulates the homeostatic proliferation and niche size of CD4+ T cells. Nat Immunol 10(2):149-57. [PubMed: 19136960] [MGI Ref ID J:144504]
Hapfelmeier S; Muller AJ; Stecher B; Kaiser P; Barthel M; Endt K; Eberhard M; Robbiani R; Jacobi CA; Heikenwalder M; Kirschning C; Jung S; Stallmach T; Kremer M; Hardt WD. 2008. Microbe sampling by mucosal dendritic cells is a discrete, MyD88-independent step in DeltainvG S. Typhimurium colitis. J Exp Med 205(2):437-50. [PubMed: 18268033] [MGI Ref ID J:132107]
Harbers SO; Crocker A; Catalano G; D'Agati V; Jung S; Desai DD; Clynes R. 2007. Antibody-enhanced cross-presentation of self antigen breaks T cell tolerance. J Clin Invest 117(5):1361-9. [PubMed: 17446931] [MGI Ref ID J:122114]
He LZ; Crocker A; Lee J; Mendoza-Ramirez J; Wang XT; Vitale LA; O'Neill T; Petromilli C; Zhang HF; Lopez J; Rohrer D; Keler T; Clynes R. 2007. Antigenic targeting of the human mannose receptor induces tumor immunity. J Immunol 178(10):6259-67. [PubMed: 17475854] [MGI Ref ID J:146119]
Hebel K; Griewank K; Inamine A; Chang HD; Muller-Hilke B; Fillatreau S; Manz RA; Radbruch A; Jung S. 2006. Plasma cell differentiation in T-independent type 2 immune responses is independent of CD11c(high) dendritic cells. Eur J Immunol 36(11):2912-9. [PubMed: 17051619] [MGI Ref ID J:117008]
Hochweller K; Striegler J; Hammerling GJ; Garbi N. 2008. A novel CD11c.DTR transgenic mouse for depletion of dendritic cells reveals their requirement for homeostatic proliferation of natural killer cells. Eur J Immunol 38(10):2776-83. [PubMed: 18825750] [MGI Ref ID J:143470]
Kassim SH; Rajasagi NK; Zhao X; Chervenak R; Jennings SR. 2006. In vivo ablation of CD11c-positive dendritic cells increases susceptibility to herpes simplex virus type 1 infection and diminishes NK and T-cell responses. J Virol 80(8):3985-93. [PubMed: 16571815] [MGI Ref ID J:144132]
Kirby AC; Beattie L; Maroof A; van Rooijen N; Kaye PM. 2009. SIGNR1-negative red pulp macrophages protect against acute streptococcal sepsis after Leishmania donovani-induced loss of marginal zone macrophages. Am J Pathol 175(3):1107-15. [PubMed: 19644016] [MGI Ref ID J:152907]
Kool M; Soullie T; van Nimwegen M; Willart MA; Muskens F; Jung S; Hoogsteden HC; Hammad H; Lambrecht BN. 2008. Alum adjuvant boosts adaptive immunity by inducing uric acid and activating inflammatory dendritic cells. J Exp Med 205(4):869-82. [PubMed: 18362170] [MGI Ref ID J:133978]
Kuwajima S; Sato T; Ishida K; Tada H; Tezuka H; Ohteki T. 2006. Interleukin 15-dependent crosstalk between conventional and plasmacytoid dendritic cells is essential for CpG-induced immune activation. Nat Immunol 7(7):740-6. [PubMed: 16715101] [MGI Ref ID J:112665]
Lee PY; Weinstein JS; Nacionales DC; Scumpia PO; Li Y; Butfiloski E; van Rooijen N; Moldawer L; Satoh M; Reeves WH. 2008. A Novel Type I IFN-Producing Cell Subset in Murine Lupus. J Immunol 180(7):5101-8. [PubMed: 18354236] [MGI Ref ID J:133377]
Liu CH; Fan YT; Dias A; Esper L; Corn RA; Bafica A; Machado FS; Aliberti J. 2006. Cutting edge: dendritic cells are essential for in vivo IL-12 production and development of resistance against Toxoplasma gondii infection in mice. J Immunol 177(1):31-5. [PubMed: 16785494] [MGI Ref ID J:134413]
Liu Z; Noh HS; Chen J; Kim JH; Falo LD Jr; You Z. 2008. Potent tumor-specific protection ignited by adoptively transferred CD4+ T cells. J Immunol 181(6):4363-70. [PubMed: 18768895] [MGI Ref ID J:139077]
Lucas M; Schachterle W; Oberle K; Aichele P; Diefenbach A. 2007. Dendritic cells prime natural killer cells by trans-presenting interleukin 15. Immunity 26(4):503-17. [PubMed: 17398124] [MGI Ref ID J:123584]
Markey KA; Banovic T; Kuns RD; Olver SD; Don AL; Raffelt NC; Wilson YA; Raggatt LJ; Pettit AR; Bromberg JS; Hill GR; MacDonald KP. 2009. Conventional dendritic cells are the critical donor APC presenting alloantigen after experimental bone marrow transplantation. Blood 113(22):5644-9. [PubMed: 19336758] [MGI Ref ID J:148895]
Miyake Y; Asano K; Kaise H; Uemura M; Nakayama M; Tanaka M. 2007. Critical role of macrophages in the marginal zone in the suppression of immune responses to apoptotic cell-associated antigens. J Clin Invest 117(8):2268-78. [PubMed: 17657313] [MGI Ref ID J:123958]
Mortier E; Woo T; Advincula R; Gozalo S; Ma A. 2008. IL-15Ralpha chaperones IL-15 to stable dendritic cell membrane complexes that activate NK cells via trans presentation. J Exp Med 205(5):1213-25. [PubMed: 18458113] [MGI Ref ID J:136221]
Mott KR; Underhill D; Wechsler SL; Ghiasi H. 2008. Lymphoid-related CD11c+ CD8alpha+ dendritic cells are involved in enhancing herpes simplex virus type 1 latency. J Virol 82(20):9870-9. [PubMed: 18667491] [MGI Ref ID J:153404]
Murillo O; Dubrot J; Palazon A; Arina A; Azpilikueta A; Alfaro C; Solano S; Ochoa MC; Berasain C; Gabari I; Perez-Gracia JL; Berraondo P; Hervas-Stubbs S; Melero I. 2009. In vivo depletion of DC impairs the anti-tumor effect of agonistic anti-CD137 mAb. Eur J Immunol 39(9):2424-36. [PubMed: 19662633] [MGI Ref ID J:152180]
Nakano H; Lin KL; Yanagita M; Charbonneau C; Cook DN; Kakiuchi T; Gunn MD. 2009. Blood-derived inflammatory dendritic cells in lymph nodes stimulate acute T helper type 1 immune responses. Nat Immunol 10(4):394-402. [PubMed: 19252492] [MGI Ref ID J:148008]
Obeid M; Tesniere A; Ghiringhelli F; Fimia GM; Apetoh L; Perfettini JL; Castedo M; Mignot G; Panaretakis T; Casares N; Metivier D; Larochette N; van Endert P; Ciccosanti F; Piacentini M; Zitvogel L; Kroemer G. 2007. Calreticulin exposure dictates the immunogenicity of cancer cell death. Nat Med 13(1):54-61. [PubMed: 17187072] [MGI Ref ID J:140379]
Ohteki T; Tada H; Ishida K; Sato T; Maki C; Yamada T; Hamuro J; Koyasu S. 2006. Essential roles of DC-derived IL-15 as a mediator of inflammatory responses in vivo. J Exp Med 203(10):2329-38. [PubMed: 16966429] [MGI Ref ID J:124624]
Osterholzer JJ; Milam JE; Chen GH; Toews GB; Huffnagle GB; Olszewski MA. 2009. Role of dendritic cells and alveolar macrophages in regulating early host defense against pulmonary infection with Cryptococcus neoformans. Infect Immun 77(9):3749-58. [PubMed: 19564388] [MGI Ref ID J:152249]
Padilla J; Daley E; Chow A; Robinson K; Parthasarathi K; McKenzie AN; Tschernig T; Kurup VP; Donaldson DD; Grunig G. 2005. IL-13 regulates the immune response to inhaled antigens. J Immunol 174(12):8097-105. [PubMed: 15944318] [MGI Ref ID J:100867]
Patsouris D; Li PP; Thapar D; Chapman J; Olefsky JM; Neels JG. 2008. Ablation of CD11c-positive cells normalizes insulin sensitivity in obese insulin resistant animals. Cell Metab 8(4):301-9. [PubMed: 18840360] [MGI Ref ID J:143426]
Plaks V; Birnberg T; Berkutzki T; Sela S; BenYashar A; Kalchenko V; Mor G; Keshet E; Dekel N; Neeman M; Jung S. 2008. Uterine DCs are crucial for decidua formation during embryo implantation in mice. J Clin Invest 118(12):3954-65. [PubMed: 19033665] [MGI Ref ID J:144732]
Poeck H; Besch R; Maihoefer C; Renn M; Tormo D; Morskaya SS; Kirschnek S; Gaffal E; Landsberg J; Hellmuth J; Schmidt A; Anz D; Bscheider M; Schwerd T; Berking C; Bourquin C; Kalinke U; Kremmer E; Kato H; Akira S; Meyers R; Hacker G; Neuenhahn M; Busch D;Ruland J; Rothenfusser S; Prinz M; Hornung V; Endres S; Tuting T; Hartmann G. 2008. 5'-Triphosphate-siRNA: turning gene silencing and Rig-I activation against melanoma. Nat Med 14(11):1256-63. [PubMed: 18978796] [MGI Ref ID J:143226]
Probst HC; Tschannen K; Odermatt B; Schwendener R; Zinkernagel RM; Van Den Broek M. 2005. Histological analysis of CD11c-DTR/GFP mice after in vivo depletion of dendritic cells. Clin Exp Immunol 141(3):398-404. [PubMed: 16045728] [MGI Ref ID J:106223]
Probst HC; van den Broek M. 2005. Priming of CTLs by lymphocytic choriomeningitis virus depends on dendritic cells. J Immunol 174(7):3920-4. [PubMed: 15778347] [MGI Ref ID J:97970]
Qiu X; Zhu L; Pollard JW. 2009. Colony-stimulating factor-1-dependent macrophage functions regulate the maternal decidua immune responses against Listeria monocytogenes infections during early gestation in mice. Infect Immun 77(1):85-97. [PubMed: 18852237] [MGI Ref ID J:144590]
Salazar-Gonzalez RM; Niess JH; Zammit DJ; Ravindran R; Srinivasan A; Maxwell JR; Stoklasek T; Yadav R; Williams IR; Gu X; McCormick BA; Pazos MA; Vella AT; Lefrancois L; Reinecker HC; McSorley SJ. 2006. CCR6-mediated dendritic cell activation of pathogen-specific T cells in Peyer's patches. Immunity 24(5):623-32. [PubMed: 16713979] [MGI Ref ID J:113363]
Sapoznikov A; Fischer JA; Zaft T; Krauthgamer R; Dzionek A; Jung S. 2007. Organ-dependent in vivo priming of naive CD4+, but not CD8+, T cells by plasmacytoid dendritic cells. J Exp Med 204(8):1923-33. [PubMed: 17646404] [MGI Ref ID J:125954]
Sapoznikov A; Pewzner-Jung Y; Kalchenko V; Krauthgamer R; Shachar I; Jung S. 2008. Perivascular clusters of dendritic cells provide critical survival signals to B cells in bone marrow niches. Nat Immunol 9(4):388-95. [PubMed: 18311142] [MGI Ref ID J:133263]
Saxena V; Ondr JK; Magnusen AF; Munn DH; Katz JD. 2007. The countervailing actions of myeloid and plasmacytoid dendritic cells control autoimmune diabetes in the nonobese diabetic mouse. J Immunol 179(8):5041-53. [PubMed: 17911589] [MGI Ref ID J:137009]
Schleicher U; Liese J; Knippertz I; Kurzmann C; Hesse A; Heit A; Fischer JA; Weiss S; Kalinke U; Kunz S; Bogdan C. 2007. NK cell activation in visceral leishmaniasis requires TLR9, myeloid DCs, and IL-12, but is independent of plasmacytoid DCs. J Exp Med 204(4):893-906. [PubMed: 17389237] [MGI Ref ID J:125611]
Schmieg J; Yang G; Franck RW; Van Rooijen N; Tsuji M. 2005. Glycolipid presentation to natural killer T cells differs in an organ-dependent fashion. Proc Natl Acad Sci U S A 102(4):1127-32. [PubMed: 15644449] [MGI Ref ID J:96123]
Scholz J; Lukacs-Kornek V; Engel DR; Specht S; Kiss E; Eitner F; Floege J; Groene HJ; Kurts C. 2008. Renal dendritic cells stimulate IL-10 production and attenuate nephrotoxic nephritis. J Am Soc Nephrol 19(3):527-37. [PubMed: 18235094] [MGI Ref ID J:150170]
Scumpia PO; McAuliffe PF; O'Malley KA; Ungaro R; Uchida T; Matsumoto T; Remick DG; Clare-Salzler MJ; Moldawer LL; Efron PA. 2005. CD11c+ dendritic cells are required for survival in murine polymicrobial sepsis. J Immunol 175(5):3282-6. [PubMed: 16116220] [MGI Ref ID J:113232]
Shaw CA; Starnbach MN. 2006. Stimulation of CD8+ T cells following diphtheria toxin-mediated antigen delivery into dendritic cells. Infect Immun 74(2):1001-8. [PubMed: 16428746] [MGI Ref ID J:105010]
Terme M; Mignot G; Ullrich E; Bonmort M; Minard-Colin V; Jacquet A; Schultze JL; Kroemer G; Leclerc C; Chaput N; Zitvogel L. 2009. The dendritic cell-like functions of IFN-producing killer dendritic cells reside in the CD11b+ subset and are licensed by tumor cells. Cancer Res 69(16):6590-7. [PubMed: 19679551] [MGI Ref ID J:151930]
Tian T; Woodworth J; Skold M; Behar SM. 2005. In vivo depletion of CD11c+ cells delays the CD4+ T cell response to Mycobacterium tuberculosis and exacerbates the outcome of infection. J Immunol 175(5):3268-72. [PubMed: 16116218] [MGI Ref ID J:113231]
Vallon-Eberhard A; Landsman L; Yogev N; Verrier B; Jung S. 2006. Transepithelial pathogen uptake into the small intestinal lamina propria. J Immunol 176(4):2465-9. [PubMed: 16456006] [MGI Ref ID J:106225]
Varol C; Landsman L; Fogg DK; Greenshtein L; Gildor B; Margalit R; Kalchenko V; Geissmann F; Jung S. 2007. Monocytes give rise to mucosal, but not splenic, conventional dendritic cells. J Exp Med 204(1):171-80. [PubMed: 17190836] [MGI Ref ID J:125312]
Varol C; Vallon-Eberhard A; Elinav E; Aychek T; Shapira Y; Luche H; Fehling HJ; Hardt WD; Shakhar G; Jung S. 2009. Intestinal lamina propria dendritic cell subsets have different origin and functions. Immunity 31(3):502-12. [PubMed: 19733097] [MGI Ref ID J:152689]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, this strain is maintained as a hemizygote. Coat color expected from breeding:Black Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $239.00 Female or Male Hemizygous for Tg(Itgax-DTR/EGFP)57Lan
Pairs /Price (US dollars $) Pair Genotype $256.75 C57BL/6J (000664) x Hemizygous for Tg(Itgax-DTR/EGFP)57Lan
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $310.70 Female or Male Hemizygous for Tg(Itgax-DTR/EGFP)57Lan
Pairs /Price (US dollars $) Pair Genotype $333.80 C57BL/6J (000664) x Hemizygous for Tg(Itgax-DTR/EGFP)57Lan
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.