Strain Name:

129S-Tnp1tm1Mlm/J

Stock Number:

004511

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Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating InvestigatorDr. Marvin L. Meistrich,   University of Texas M.D. Anderson Cancer

Description
Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected in testicular homogenates. Levels of transition nuclear protein 2 and protamine 2 precursor are elevated. Homozygous female mice are fertile. Homozygous male mice are subfertile, approximately 60% are infertile, and display diminished sperm motility, sperm tail abnormalities and sperm chromatin abnormalities. Electron microscopy analysis of spermatids reveals both abnormal large rod-like chromatin condensation units as well as normal fine fibrillar chromatin morphology. This mutant mouse strain may be useful in studies of male infertility and/or subfertility.

Development
A targeting vector containing neomycin resistance and thymidine kinase genes was used to replace the entire targeted gene. The construct was electroporated into 129S7/SvEvBrd-Hprt+ derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were backcrossed to 129S6/SvEvTac female mice.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Tnp1tm1Mlm/Tnp1tm1Mlm

        involves: 129S7/SvEvBrd * C57BL/6J
  • reproductive system phenotype
  • *normal* reproductive system phenotype
    • homozygotes exhibit no differences in testis and seminal vesicle weights and sperm counts relative to wild-type and heterozygous controls   (MGI Ref ID J:61941)
    • abnormal sperm nucleus morphology
      • homozygotes display a significantly higher frequency of epididymal sperm heads in which the nuclear apex is bent or blunted   (MGI Ref ID J:61941)
      • large rod-like chromatin condensation units are observed in nuclei of mutant step 13 spermatids, unlike the fine chromatin fibrils observed in wild type; after step 14, all of the chromatin becomes condensed and darkly stained with hematoxylin   (MGI Ref ID J:61941)
    • abnormal spermatid morphology
      • EM revealed large rod-like chromatin condensation units in the nuclei of mutant step 13 spermatids, in contrast to the fine chromatin fibrils observed in wild type   (MGI Ref ID J:61941)
    • abnormal spermiogenesis
      • in steps 12 and 13, mutant spermatid nuclei exhibit increased levels of transition protein 2 (TNP2), accounting for 91% of the basic nuclear protein, as well as increased levels of protamine 2 precursor pP2 (2%-11% vs 0% in controls); in contrast, histone displacement occurs normally   (MGI Ref ID J:61941)
      • in steps 14-16 spermatids, pP2 is 21% of the total nuclear protein relative to only 10% and 5% in heterozygous and wild-type males, respectively, and the ratio of pP2 to pP2/11 is increased, suggesting abnormal P2 processing   (MGI Ref ID J:61941)
      • although most of the precursor is processed to mature P2, high levels of incompletely processed forms pesist in epididymal spermatozoa   (MGI Ref ID J:61941)
    • asthenozoospermia
      • only 23% of mutant sperm exhibit any motility   (MGI Ref ID J:61941)
      • most of these sperm fail to show forward progression   (MGI Ref ID J:61941)
    • coiled sperm flagellum
      • mutant sperm with tails coiled around the heads are observed   (MGI Ref ID J:61941)
      • however, most cauda epididymal sperm appear relatively normal   (MGI Ref ID J:61941)
    • decreased litter size
      • whereas fertile B6 x 129 F2 male homozygotes (16 weeks of age) exhibit normal litter sizes relative to wild-type controls, those on an inbred 129 background (9 weeks of age) display a 70% reduction in litter size relative to wild-type and heterozygous controls   (MGI Ref ID J:61941)
    • male infertility
      • 70% of male homozygotes are infertile on a hybrid genetic background (B6 x 129 F2) versus 60% on an inbred 129 background   (MGI Ref ID J:61941)
    • reduced male fertility
      • fertile homozygotes on both the B6 129 F2 and 129 backgrounds produce a reduced number of litters/pair relative to wild-type controls   (MGI Ref ID J:61941)
  • cellular phenotype
  • coiled sperm flagellum
    • mutant sperm with tails coiled around the heads are observed   (MGI Ref ID J:61941)
    • however, most cauda epididymal sperm appear relatively normal   (MGI Ref ID J:61941)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Tnp1tm1Mlm related

Reproductive Biology Research
Fertility Defects
      males only

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tnp1tm1Mlm
Allele Name targeted mutation 1, Marvin L Meistrich
Allele Type Targeted (Null/Knockout)
Common Name(s) Tnp1-; Tnp1-null;
Mutation Made ByDr. Marvin Meistrich,   University of Texas M.D. Anderson Cancer
Strain of Origin129S7/SvEvBrd-Hprt<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt<+>
Gene Symbol and Name Tnp1, transition protein 1
Chromosome 1
Gene Common Name(s) Stp-1; TP1; Tp-1; spermatid transition protein 1;
Molecular Note The entire gene was replaced with a neomycin cassette, which was inserted in an antisense orientation. Tnp1 protein was not detectable in extracts of testicular homogenates from homozygous mutant mice. [MGI Ref ID J:61941]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Yu YE; Zhang Y; Unni E; Shirley CR; Deng JM; Russell LD; Weil MM; Behringer RR; Meistrich ML. 2000. Abnormal spermatogenesis and reduced fertility in transition nuclear protein 1-deficient mice. Proc Natl Acad Sci U S A 97(9):4683-8. [PubMed: 10781074]  [MGI Ref ID J:61941]

Additional References

Zhao M; Shirley CR; Hayashi S; Marcon L; Mohapatra B; Suganuma R; Behringer RR; Boissonneault G; Yanagimachi R; Meistrich ML. 2004. Transition nuclear proteins are required for normal chromatin condensation and functional sperm development. Genesis 38(4):200-13. [PubMed: 15083521]  [MGI Ref ID J:89779]

Tnp1tm1Mlm related

Liebe B; Petukhova G; Barchi M; Bellani M; Braselmann H; Nakano T; Pandita TK; Jasin M; Fornace A; Meistrich ML; Baarends WM; Schimenti J; de Lange T; Keeney S; Camerini-Otero RD; Scherthan H. 2006. Mutations that affect meiosis in male mice influence the dynamics of the mid-preleptotene and bouquet stages. Exp Cell Res 312(19):3768-81. [PubMed: 17010969]  [MGI Ref ID J:147391]

Shirley CR; Hayashi S; Mounsey S; Yanagimachi R; Meistrich ML. 2004. Abnormalities and reduced reproductive potential of sperm from Tnp1- and Tnp2-null double mutant mice. Biol Reprod 71(4):1220-9. [PubMed: 15189834]  [MGI Ref ID J:93181]

Suganuma R; Yanagimachi R; Meistrich ML. 2005. Decline in fertility of mouse sperm with abnormal chromatin during epididymal passage as revealed by ICSI. Hum Reprod 20(11):3101-8. [PubMed: 16037114]  [MGI Ref ID J:106637]

Zhao M; Shirley CR; Hayashi S; Marcon L; Mohapatra B; Suganuma R; Behringer RR; Boissonneault G; Yanagimachi R; Meistrich ML. 2004. Transition nuclear proteins are required for normal chromatin condensation and functional sperm development. Genesis 38(4):200-13. [PubMed: 15083521]  [MGI Ref ID J:89779]

Zhao M; Shirley CR; Mounsey S; Meistrich ML. 2004. Nucleoprotein transitions during spermiogenesis in mice with transition nuclear protein Tnp1 and Tnp2 mutations. Biol Reprod 71(3):1016-25. [PubMed: 15163613]  [MGI Ref ID J:107120]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain originated on a 129S7 background, has been backcrossed to the 129S6/SvEvTac background and is maintained as heterozygous males and homozygous females.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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