Description

Strain Information

Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse   Donating Investigator Marvin Meistrich,   University of Texas M.D. Anderson Cancer

Description
Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected in testicular homogenates. Levels of transition nuclear protein 2 and protamine 2 precursor are elevated. Homozygous female mice are fertile. Homozygous male mice are subfertile, approximately 60% are infertile, and display diminished sperm motility, sperm tail abnormalities and sperm chromatin abnormalities. Electron microscopy analysis of spermatids reveals both abnormal large rod-like chromatin condensation units as well as normal fine fibrillar chromatin morphology. This mutant mouse strain may be useful in studies of male infertility and/or subfertility.

Development
A targeting vector containing neomycin resistance and thymidine kinase genes was used to replace the entire targeted gene. The construct was electroporated into 129S7/SvEvBrd-Hprt⁺ derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were backcrossed to 129S6/SvEvTac female mice.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tnp1^tm1Mlm/Tnp1^tm1Mlm

        involves: 129S7/SvEvBrd * C57BL/6J

• reproductive system phenotype
• abnormal spermatogenesis (MGI Ref ID J:61941)
• asthenozoospermia (MGI Ref ID J:61941)
  • impaired sperm motility
• reduced male fertility (MGI Ref ID J:61941)
  • ~60% of males were infertile

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Tnp1^tm1Mlm related

Reproductive Biology Research
Fertility Defects (males only)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tnp1tm1Mlm
Allele Name		targeted mutation 1, Marvin L Meistrich
Allele Type		Targeted (knock-out)
Common Name(s)		Tnp1-; Tnp1-null;
Mutation Made By		Marvin Meistrich,   University of Texas M.D. Anderson Cancer
Strain of Origin		129S7/SvEvBrd-Hprt1+
ES Cell Line Name		AB1
ES Cell Line Strain		129S7/SvEvBrd-Hprt1<+>
Gene Symbol and Name		Tnp1, transition protein 1
Chromosome		1
Gene Common Name(s)		Stp-1; TP1; Tp-1; spermatid transition protein 1;
Molecular Note		The entire gene was replaced with a neomycin cassette, which was inserted in an antisense orientation. Tnp1 protein was not detectable in extracts of testicular homogenates from homozygous mutant mice. [MGI Ref ID J:61941]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Yu YE; Zhang Y; Unni E; Shirley CR; Deng JM; Russell LD; Weil MM; Behringer RR; Meistrich ML. 2000. Abnormal spermatogenesis and reduced fertility in transition nuclear protein 1-deficient mice. Proc Natl Acad Sci U S A 97(9):4683-8. [PubMed: 10781074]  [MGI Ref ID J:61941]

Additional References

Zhao M; Shirley CR; Hayashi S; Marcon L; Mohapatra B; Suganuma R; Behringer RR; Boissonneault G; Yanagimachi R; Meistrich ML. 2004. Transition nuclear proteins are required for normal chromatin condensation and functional sperm development. Genesis 38(4):200-13. [PubMed: 15083521]  [MGI Ref ID J:89779]

Tnp1^tm1Mlm related

Shirley CR; Hayashi S; Mounsey S; Yanagimachi R; Meistrich ML. 2004. Abnormalities and reduced reproductive potential of sperm from Tnp1- and Tnp2-null double mutant mice. Biol Reprod 71(4):1220-9. [PubMed: 15189834]  [MGI Ref ID J:93181]

Suganuma R; Yanagimachi R; Meistrich ML. 2005. Decline in fertility of mouse sperm with abnormal chromatin during epididymal passage as revealed by ICSI. Hum Reprod 20(11):3101-8. [PubMed: 16037114]  [MGI Ref ID J:106637]

Zhao M; Shirley CR; Hayashi S; Marcon L; Mohapatra B; Suganuma R; Behringer RR; Boissonneault G; Yanagimachi R; Meistrich ML. 2004. Transition nuclear proteins are required for normal chromatin condensation and functional sperm development. Genesis 38(4):200-13. [PubMed: 15083521]  [MGI Ref ID J:89779]

Zhao M; Shirley CR; Mounsey S; Meistrich ML. 2004. Nucleoprotein transitions during spermiogenesis in mice with transition nuclear protein Tnp1 and Tnp2 mutations. Biol Reprod 71(3):1016-25. [PubMed: 15163613]  [MGI Ref ID J:107120]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	This strain originated on a 129S7 background, has been backcrossed to the 129S6/SvEvTac background and is maintained as heterozygous males and homozygous females.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

Terms of Use

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JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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