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Strain Name:

C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J

Stock Number:

004512

Availability:

Repository- Live

Price and Supply Information

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Additional Licensing and Use Restrictions

Former Name      C.FVB-Tg(Itgax-DTR/GFP)57Drl    (Changed: 15-DEC-04 )
      C.FVB-Tg(Itgax-DTR/GFP)57Lan/J    (Changed: 15-DEC-04 )
      C.FVB-Tg(Itgax-DTR/GFP)57Litt    (Changed: 15-DEC-04 )
      CD11c-DTR    (Changed: 15-DEC-04 )
Genes & Alleles   GFP;   Itgax;   DTR;   Tg(Itgax-DTR/EGFP)57Lan;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating SystemInbred x Hemizygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Dan Littman,   New York University Medical Center
GenerationN8+6F1N3 (08-JAN-08)

Strain Description
Mice that are homozygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. Upon diphtheria toxin (DT) administration, mice harboring this transgene are transiently depleted of dendritic cell (DC) populations. All CD8+ and CD8- DC in the spleen express EGFP and are DT sensitive. Immunohistochemical and flow cytometric analysis reveals EGFP expression and DT-inducible depletion of CD11chigh DC in spleen, lymph node, lung, liver and lamina propria tissues, as well as the defined macrophage subpopulations of the alveolar, lamina propria, metallophillic and marginal zone. Rapid reduction of CD11c+ DC populations induced by intraperitoneal injection of DT persists for approximately 2 days, after which the cell population gradually recovers. While transient DC depletion was not associated with sign of illness or long-term defects, repeated DT induction is lethal to the mouse. Long term depletion of this cell population can be achieved by the generation of mixed irradiation chimeras. This mutant mouse strain may be useful in studies of mononuclear phagocyte origins and the specific role of dendritic cells in the immune response.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Strain Development
A transgene was designed to place a simian Diphtheria Toxin Receptor (DTR)-Enhanced Green Fluorescent Protein (EGFP, Stratagene) fusion protein under the control of the Itgax (or CD11c) promoter. This transgene was introduced into fertilized FVB/N donor eggs. The resulting chimeric animals were crossed to BALB/c mice, and then backcrossed to BALB/c for 8 generations. This transgene has been mapped to mouse Chromosome 1 (~52 cM; 87395187bp position) via single nucleotide polymorphism (SNP) analysis by The Jackson Laboratory.

Mammalian Phenotype Terms assigned by genotype

Tg(Itgax-DTR/EGFP)57Lan/0

        C.FVB-Tg(Itgax-DTR/EGFP)57Lan   (conditional)
  • life span-post-weaning/aging
  • premature death (MGI Ref ID J:93488)
    • repeated treatment with diphtheria toxin results in lethality due to dendritic cell depletion
  • immune system phenotype
  • abnormal CD8-positive T cell differentiation (MGI Ref ID J:93488)
    • adoptive transfer experiments demonstrate that CD8+ T cell priming with cell-associated antigen does not occur in diphtheria-induced dendritic cell depleted mice
  • abnormal T cell activation (MGI Ref ID J:93488)
    • treatment with diphtheria toxin results in a loss of in vitro stimulation of alloreactive T cells
  • decreased CD8-positive T cell number (MGI Ref ID J:93488)
    • treatment with diphtheria toxin results in a substantial loss of CD8+ T cells
  • decreased dendritic cell number (MGI Ref ID J:93488)
    • CD11c+ (Itgax+) dendritic cells are depleted for 2 days following treatment with diphtheria toxin
  • defective cytotoxic T cell cytolysis (MGI Ref ID J:93488)
    • in adoptive transfer experiments using diphtheria-induced dendritic cell depleted mice infected with Listeria or malaria at the liver stage
  • hematopoietic system phenotype
  • abnormal CD8-positive T cell differentiation (MGI Ref ID J:93488)
    • adoptive transfer experiments demonstrate that CD8+ T cell priming with cell-associated antigen does not occur in diphtheria-induced dendritic cell depleted mice
  • abnormal T cell activation (MGI Ref ID J:93488)
    • treatment with diphtheria toxin results in a loss of in vitro stimulation of alloreactive T cells
  • decreased CD8-positive T cell number (MGI Ref ID J:93488)
    • treatment with diphtheria toxin results in a substantial loss of CD8+ T cells
  • decreased dendritic cell number (MGI Ref ID J:93488)
    • CD11c+ (Itgax+) dendritic cells are depleted for 2 days following treatment with diphtheria toxin

Tg(Itgax-DTR/EGFP)57Lan/0

        C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J   (conditional)
  • immune system phenotype
  • *normal* immune system phenotype (MGI Ref ID J:96123)
    • conventional T cell, macrophages, B cell, NK cell, and NK T cell numbers are unaffected by treatment with diphtheria toxin
    • abnormal NK cell physiology (MGI Ref ID J:96123)
      • following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin
    • decreased circulating interferon-gamma level (MGI Ref ID J:96123)
      • following treatment with diphtheria toxin, little IFN-gamma in alpha-GalCer-injected mice and no IFN-gamma in alpha-C-GalCer-injected mice was detected unlike in transgenic mice not treated with diphtheria toxin
    • decreased circulating interleukin-12 level (MGI Ref ID J:96123)
      • following treatment with diphtheria toxin and stimulation with alpha-GalCer or alpha-C-GalCer, virtually no serum IL-12 was detected unlike in transgenic mice not treated with diphtheria toxin
    • decreased dendritic cell number (MGI Ref ID J:96123)
      • 24 hours after treatment with diphtheria toxin, spleens and livers are depleted of dendritic cells
    • decreased interferon-gamma secretion (MGI Ref ID J:96123)
      • following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin
  • homeostasis/metabolism phenotype
  • decreased circulating interferon-gamma level (MGI Ref ID J:96123)
    • following treatment with diphtheria toxin, little IFN-gamma in alpha-GalCer-injected mice and no IFN-gamma in alpha-C-GalCer-injected mice was detected unlike in transgenic mice not treated with diphtheria toxin
  • decreased circulating interleukin-12 level (MGI Ref ID J:96123)
    • following treatment with diphtheria toxin and stimulation with alpha-GalCer or alpha-C-GalCer, virtually no serum IL-12 was detected unlike in transgenic mice not treated with diphtheria toxin
  • hematopoietic system phenotype
  • decreased dendritic cell number (MGI Ref ID J:96123)
    • 24 hours after treatment with diphtheria toxin, spleens and livers are depleted of dendritic cells

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(Itgax-DTR/EGFP)57Lan/0

        B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J   (conditional)
  • life span-post-weaning/aging
  • abnormal induced morbidity/mortality (MGI Ref ID J:113232)
    • mice exhibit an increase in mortality compared to wild type mice following diphtheria treatment and cecal ligation puncture (CLP) (100% compared to 45% mortality)
    • however, replacement of dendritic cells with wild type dendritic cells improves survival (mortality drops from 100% to 65%)
  • immune system phenotype
  • *normal* immune system phenotype (MGI Ref ID J:122114)
    • depletion of Cd11c+ dendritic cells by treatment with diphtheria toxin does not affect accumulation and localization of Tg(TcraTcrb)1100Mjb CD8+ T cells from a Rag1 null mouse in T cell zones of the lymph node and spleen
    • conventional T cell, macrophages, B cell, NK cell, and NK T cell numbers are unaffected by treatment with diphtheria toxin
    • despite an increase in mortality following diphtheria treatment and cecal ligation puncture, mice do not exhibit an increase in bacteremia or cytokine production
    • abnormal NK T cell physiology (MGI Ref ID J:96123)
      • following treatment with diphtheria toxin and stimulation with alpha-GalCer, 5% of spleen NK T cells stain positive for IFN-gamma and 8% for IL-4 compared to 32% and 30%, respectively, of similarly treated wild type NK T cells
      • however, normal levels of IFN-gamma and IL-4 production where observed in liver NK T cells
    • abnormal NK cell physiology (MGI Ref ID J:96123)
      • following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin
      • following treatment with diphtheria toxin and stimulation with alpha-C-GalCer, less than 1% at 6 and 0.5% to 1% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 6% to 8% and 12% to 14%, respectively, of cells from transgenic mice not treated with diphtheria toxin
      • impaired NK cell cytolysis (MGI Ref ID J:125611)
        • unlike in wild type mice, NK T cell activation measured by specific cell lysis following infection with Leishmania infantum in diphtheria toxin treated mice does not occur
    • decreased circulating interferon-gamma level (MGI Ref ID J:96123)
      • following treatment with diphtheria toxin, little IFN-gamma in alpha-GalCer-injected mice and no IFN-gamma in alpha-C-GalCer-injected mice was detected unlike in transgenic mice not treated with diphtheria toxin
    • decreased circulating interleukin-12 level (MGI Ref ID J:96123)
      • following treatment with diphtheria toxin and stimulation with alpha-GalCer or alpha-C-GalCer, virtually no serum IL-12 was detected unlike in transgenic mice not treated with diphtheria toxin
    • decreased dendritic cell number (MGI Ref ID J:100867)
      • CD11c+ (Itgax+) dendritic cells in lung parenchyma are depleted following treatment with diphtheria toxin
      • 24 hours after treatment with diphtheria toxin, spleens and livers are depleted of dendritic cells
      • treatment with diphtheria toxin depletes CD11chighMHCIIhigh dendritic cells
    • decreased interferon-gamma secretion (MGI Ref ID J:96123)
      • following treatment with diphtheria toxin and stimulation with alpha-GalCer, only 2% at 6 hours and 4% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 20% to 23% and 13% to 16%, respectively, of cells from transgenic mice not treated with diphtheria toxin
      • following treatment with diphtheria toxin and stimulation with alpha-GalCer, 5% of spleen NK T cells stain positive for IFN-gamma compared to 32% of similarly treated wild type NK T cells
      • however, normal levels of production where observed in liver NK T cells
      • following treatment with diphtheria toxin and stimulation with alpha-C-GalCer, less than 1% at 6 and 0.5% to 1% at 12 hours post-treatment of liver or spleen NK cells stain positive for IFN-gamma compared to 6% to 8% and 12% to 14%, respectively, of cells from transgenic mice not treated with diphtheria toxin
    • decreased interleukin-12b secretion (MGI Ref ID J:125611)
      • unlike in wild type mice, IL-12p40 production following infection with Leishmania infantum in diphtheria toxin treated mice does not occur
    • decreased interleukin-4 secretion (MGI Ref ID J:96123)
      • following treatment with diphtheria toxin and stimulation with alpha-GalCer, 8% of spleen NK T cells stain positive for IL-4 compared to 30% of similarly treated wild type NK T cells however, normal levels of production where observed in liver NK T cells
    • sepsis (MGI Ref ID J:113232)
      • mice exhibit an increase in mortality compared to wild type mice following diphtheria treatment and cecal ligation puncture (CLP) (100% compared to 45% mortality)
  • homeostasis/metabolism phenotype
  • decreased circulating interferon-gamma level (MGI Ref ID J:96123)
    • following treatment with diphtheria toxin, little IFN-gamma in alpha-GalCer-injected mice and no IFN-gamma in alpha-C-GalCer-injected mice was detected unlike in transgenic mice not treated with diphtheria toxin
  • decreased circulating interleukin-12 level (MGI Ref ID J:96123)
    • following treatment with diphtheria toxin and stimulation with alpha-GalCer or alpha-C-GalCer, virtually no serum IL-12 was detected unlike in transgenic mice not treated with diphtheria toxin
  • hematopoietic system phenotype
  • decreased dendritic cell number (MGI Ref ID J:100867)
    • CD11c+ (Itgax+) dendritic cells in lung parenchyma are depleted following treatment with diphtheria toxin
    • 24 hours after treatment with diphtheria toxin, spleens and livers are depleted of dendritic cells
    • treatment with diphtheria toxin depletes CD11chighMHCIIhigh dendritic cells

Gene & Allele Details

Allele Symbol Tg(Itgax-DTR/EGFP)57Lan
Allele Name transgene insertion 57, Richard A Lang
Common Name(s) CD11c-DTR; CD11c/DTR Tg; CD11cDTR tg; DCKO; DTR-GFP; DTR-GRP; Tg(Itgax-DTR/GFP)57Lan; Tg.Itgax-DTR/EGFP.57Lan; TgItgax-DTR/EGFP.57Lan;
Mutation Made By Steffen Jung,   Weizmann Institute of Science
Strain of OriginFVB/N
Site of ExpressionExpression of the transgene is specific to splenic dendritic cells. All CD8+ and CD8- dentritic cells in the spleen fluoresce and are DT sensititve.
Expressed Gene GFP, Green Fluorescent Protein, jellyfish
Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source.
Expressed Gene DTR, Simian Diphtheria Toxin Receptor,
Promoter Itgax, integrin alpha X, mouse, laboratory
Molecular Note The transgene contains sequence encoding DTR/GFP under the control of the mouse Itgax promoter. Two lines were creacted (line 11 and line 57) with 1 to 2 copies and 20 copies of the transgene, respectively. All CD8+ and CD8- dendritic cells in the spleen fluoresce and are DT sensitive. Immunohistochemical and flow cytometric analyses show that expression of the transgene is specific to splenic dendritic cells. [MGI Ref ID J:93488]

Control Information

  Control
   Noncarrier
   000651 BALB/cJ
 
  Considerations for Choosing Controls
  Control Pricing Information for JAX® GEMM® Strains

Genotyping Protocols

Itgax-DTR/GFP

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, this strain is maintained as a hemizygote.
Diet Information LabDiet® 5K52/5K67

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View Fluorescent Protein Strains     (139 strains)

Strains carrying   Tg(Itgax-DTR/EGFP)57Lan allele
004509   B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
View Strains carrying   Tg(Itgax-DTR/EGFP)57Lan     (1 strain)

Strains carrying other alleles of GFP
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005622   B6.Cg-Shhtm1(EGFP/cre)Cjt/J
007484   B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
004178   B6.Cg-Tg(CAG-Bgeo/GFP)21Lbe/J
007575   B6.Cg-Tg(CAG-Ngb,-EGFP)1Dgrn/J
008111   B6.Cg-Tg(CAG-Ub*G76V/GFP)1Dant/J
008112   B6.Cg-Tg(CAG-Ub*G76V/GFP)2Dant/J
006069   B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J
005029   B6.Cg-Tg(Hlxb9-GFP)1Tmj/J
006864   B6.Cg-Tg(Ins1-EGFP)1Hara/J
005244   B6.Cg-Tg(Krt1-15-EGFP)2Cot/J
007742   B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J
007902   B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J
007894   B6.Cg-Tg(Rgs4-EGFP)4Lvt/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
004659   B6.Cg-Tg(TIE2GFP)287Sato/1J
007921   B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J
006000   B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
006417   B6.FVB-Tg(Npy-hrGFP)1Lowl/J
005738   B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J
004077   B6;129-Gt(ROSA)26Sortm2Sho/J
006667   B6;129P2-Omptm3Mom/MomJ
004966   B6;CBA-Tg(Acrv1-EGFP)2727Redd/J
004654   B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J
005621   B6;D2-Tg(S100B-EGFP)1Wjt/J
004690   B6;FVB-Tg(Pcp2-EGFP)2Yuza/J
006147   B6;FVB-Tg(Sfpi1,-EGFP)7Dgt/J
006043   B6;SJL-Tg(Oxt/EGFP)AI03Wsy/J
006567   C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ
003291   C57BL/6-Tg(CAG-EGFP)1Osb/J
005070   C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
007265   C57BL/6-Tg(Sry-EGFP)92Ei Chr YAKR/J/EiJ
007264   C57BL/6-Tg(Sry-EGFP)92Ei Tg(Sry)4Ei Chr YPOS/EiJ
004353   C57BL/6-Tg(UBC-GFP)30Scha/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
003927   C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ
007677   CB6-Tg(Gad1-EGFP)G42Zjh/J
007076   CByJ.B6-Tg(UBC-GFP)30Scha/J
003718   FVB-Tg(GadGFP)45704Swn/J
005515   FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
006421   FVB-Tg(Pomc1-hrGFP)1Lowl/J
003516   FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
007483   FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
003257   FVB/N-Tg(GFAPGFP)14Mes/J
008173   NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
005076   NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
005082   NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ
005334   NOD/ShiLt-Tg(Cd4-EGFP)1Lt/J
005282   NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
006741   STOCK Olfr160tm1Mom Tg(Olfr151,taulacZ)BMom/MomJ
006770   STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J
006570   STOCK Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj Tg(SMN2)89Ahmb/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
005105   STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J
005854   STOCK Tg(Cp-EGFP)25Gaia/J
006334   STOCK Tg(Gad1-EGFP)94Agmo/J
006340   STOCK Tg(Gad1-EGFP)98Agmo/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
005418   STOCK Tg(HIST1H2BB/EGFP)1Pa/J
003658   STOCK Tg(TIE2GFP)287Sato/J
006129   STOCK Tg(Zp3-EGFP)1Dean/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
View Strains carrying other alleles of GFP     (80 strains)

Strains carrying other alleles of Itgax
008068   B6.Cg-Tg(Itgax-cre)1-1Reiz/J
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
View Strains carrying other alleles of Itgax     (2 strains)

Strains carrying other alleles of DTR
006000   B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
005515   FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
View Strains carrying other alleles of DTR     (2 strains)

Additional Web Information

Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
JAX Notes, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cancer Research (tumor immunology)
Cancer Research (xenograft/transplant host)
Fluorescent Proteins
Immunology and Inflammation Research

GFP related

Research Tools
Fluorescent Proteins

References

Selected Reference(s)

Jung S; Unutmaz D; Wong P; Sano G; De los Santos K; Sparwasser T; Wu S; Vuthoori S; Ko K; Zavala F; Pamer EG; Littman DR; Lang RA. 2002. In vivo depletion of CD11c(+) dendritic cells abrogates priming of CD8(+) T cells by exogenous cell-associated antigens. Immunity 17(2):211-20. [PubMed: 12196292]  [MGI Ref ID J:93488]

Additional References

Price and Supply Information

Strain Name: C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
Stock Number: 004512

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:

*Pricing for Shipping Destination selected:

        USA, Canada and Mexico

Price(s) in US dollars ($)Genotype(s) Provided
Individual Mouse Price $236.40Hemizygous for Tg(Itgax-DTR/EGFP)57Lan
Pair $254.40BALB/cJ (000651) x Hemizygous for Tg(Itgax-DTR/EGFP)57Lan
Pair $253.85Hemizygous for Tg(Itgax-DTR/EGFP)57Lan x BALB/cJ (000651)

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423.
Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.
View Control Pricing Information for JAX® Strains.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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