Strain Name:

B6.129S1-Bcl2l11tm1.1Ast/J

Stock Number:

004525

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Availability:

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These Bcl2l11tm1.1Ast knock-out mice exhibit progressive systemic autoimmune disease. They may be useful in studies of apoptosis, degenerative and autoimmune diseases, including lupus erythematosus and autoimmune kidney disease.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
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Additional information on Congenic nomenclature.
Mating SystemHeterozygote x Homozygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
GenerationN14+F8 (17-JUL-13)
Generation Definitions
 
Donating Investigator Andreas Strasser,   Walter and Eliza Hall Inst of Med Res

Description
Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. No full length gene product (protein) is immunodetected in spleen cells from homozygous mutant mice. Homozygous mice have lympho-myeloid hyperplasia and reduced platelet number. Lymphocytes are insensitive to certain apoptotic stimuli. Both homozygous and heterozygous mice exhibit progressive systemic autoimmune disease. This mutant mouse strain may be useful in studies of apoptosis, degenerative and autoimmune diseases, including lupus erythematosus and autoimmune kidney disease.

Development
A targeting vector containing a floxed neomycin resistance gene and a thymidine kinase gene was used to disrupt the exon encoding the BH3 domain. The construct was electroporated into 129S1 derived W9.5 embryonic stem (ES) cells. correctly targeted ES cells were injected into C57BL/6 blastocysts.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Bcl2l11
011026   B6.129S-Bcl2l11tm3Rjd/J
011028   B6.129S6-Bcl2l11tm1Rjd/J
011025   B6.129S6-Bcl2l11tm2Rjd/J
011027   B6.129S6-Bcl2l11tm4Rjd/J
View Strains carrying other alleles of Bcl2l11     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Bcl2l11tm1.1Ast/Bcl2l11+

        B6.129S1-Bcl2l11tm1.1Ast
  • hematopoietic system phenotype
  • increased leukocyte cell number
    • increased white blood cells numbers (<1.5 fold)   (MGI Ref ID J:153846)
  • increased spleen weight
    • increase in spleen weight (<2 fold)   (MGI Ref ID J:153846)
  • immune system phenotype
  • *normal* immune system phenotype
    • normal in vitro survival assays with several cytotoxic stimuli on sorted thymocyte and B cell populations   (MGI Ref ID J:153846)
    • increased leukocyte cell number
      • increased white blood cells numbers (<1.5 fold)   (MGI Ref ID J:153846)
    • increased spleen weight
      • increase in spleen weight (<2 fold)   (MGI Ref ID J:153846)

Bcl2l11tm1.1Ast/Bcl2l11tm1.1Ast

        B6.129S1-Bcl2l11tm1.1Ast
  • immune system phenotype
  • *normal* immune system phenotype
    • normal in vitro survival assays with several cytotoxic stimuli on sorted thymocyte and B cell populations   (MGI Ref ID J:153846)
    • abnormal B cell morphology   (MGI Ref ID J:133084)
      • increased immature B cell number   (MGI Ref ID J:133084)
        • increased transitional stage B cell number
          • T1 transitional B cells are insignificantly elevated   (MGI Ref ID J:133084)
          • increased T1 transitional B cells in inguinal lymph nodes   (MGI Ref ID J:133084)
      • increased mature B cell number   (MGI Ref ID J:133084)
    • abnormal NK cell morphology
      • following expansion in culture, NK cells survive IL-15 withdrawal better than wild-type cells   (MGI Ref ID J:123409)
      • increased NK cell number
        • mice have a higher percent of KLRG1+CD27- NK cells in the spleen, liver and bone marrow compared to wild-type mice   (MGI Ref ID J:123409)
    • abnormal leukopoiesis
      • irradiated mice reconstituted with mutant fetal liver cells have a 2- to 4- fold increase in lymphocyte and myeloid cell numbers compared to controls   (MGI Ref ID J:73316)
      • increased immature B cell number   (MGI Ref ID J:133084)
        • increased transitional stage B cell number
          • T1 transitional B cells are insignificantly elevated   (MGI Ref ID J:133084)
          • increased T1 transitional B cells in inguinal lymph nodes   (MGI Ref ID J:133084)
    • abnormal lymphocyte physiology
      • survival of mature resting T and B cells in the absence of cytokines is improved compared to wild-type cells   (MGI Ref ID J:73316)
      • decreased B cell apoptosis
        • about 10-fold more B cells cultured without cytokines are alive after six days compared to controls   (MGI Ref ID J:73316)
      • decreased T cell apoptosis
        • double positive T cells with increased resistance to the apoptotic effects of dexamethasone   (MGI Ref ID J:133084)
        • about 10-fold more T cells cultured without cytokines are alive after six days compared to controls   (MGI Ref ID J:73316)
    • abnormal response to infection
      • following infection with mouse cytomegalovirus infection, more NK cell survive at day 10 and 14 than in wild-type mice   (MGI Ref ID J:123409)
    • increased leukocyte cell number
      • increased white blood cells numbers (>3 fold)   (MGI Ref ID J:153846)
      • increased NK cell number
        • mice have a higher percent of KLRG1+CD27- NK cells in the spleen, liver and bone marrow compared to wild-type mice   (MGI Ref ID J:123409)
      • increased immature B cell number   (MGI Ref ID J:133084)
        • increased transitional stage B cell number
          • T1 transitional B cells are insignificantly elevated   (MGI Ref ID J:133084)
          • increased T1 transitional B cells in inguinal lymph nodes   (MGI Ref ID J:133084)
      • increased mature B cell number   (MGI Ref ID J:133084)
      • increased regulatory T cell number
    • increased spleen weight
      • increased spleen weight (>2.5 fold)   (MGI Ref ID J:153846)
  • homeostasis/metabolism phenotype
  • decreased physiological sensitivity to xenobiotic
    • NK cells are more resistant to LY294002-induced apoptosis   (MGI Ref ID J:123409)
  • cellular phenotype
  • decreased apoptosis
    • NK cells are more resistant to LY294002-induced apoptosis   (MGI Ref ID J:123409)
    • sympathetic neurons are partially protected from apoptosis induced by the withdrawal of nerve growth factor   (MGI Ref ID J:123409)
    • decreased B cell apoptosis
      • about 10-fold more B cells cultured without cytokines are alive after six days compared to controls   (MGI Ref ID J:73316)
    • decreased T cell apoptosis
      • double positive T cells with increased resistance to the apoptotic effects of dexamethasone   (MGI Ref ID J:133084)
      • about 10-fold more T cells cultured without cytokines are alive after six days compared to controls   (MGI Ref ID J:73316)
  • nervous system phenotype
  • abnormal sympathetic neuron morphology
    • sympathetic neurons are partially protected from apoptosis induced by the withdrawal of nerve growth factor   (MGI Ref ID J:124044)
  • hematopoietic system phenotype
  • abnormal B cell morphology   (MGI Ref ID J:133084)
    • increased immature B cell number   (MGI Ref ID J:133084)
      • increased transitional stage B cell number
        • T1 transitional B cells are insignificantly elevated   (MGI Ref ID J:133084)
        • increased T1 transitional B cells in inguinal lymph nodes   (MGI Ref ID J:133084)
    • increased mature B cell number   (MGI Ref ID J:133084)
  • abnormal NK cell morphology
    • following expansion in culture, NK cells survive IL-15 withdrawal better than wild-type cells   (MGI Ref ID J:123409)
    • increased NK cell number
      • mice have a higher percent of KLRG1+CD27- NK cells in the spleen, liver and bone marrow compared to wild-type mice   (MGI Ref ID J:123409)
  • abnormal leukopoiesis
    • irradiated mice reconstituted with mutant fetal liver cells have a 2- to 4- fold increase in lymphocyte and myeloid cell numbers compared to controls   (MGI Ref ID J:73316)
    • increased immature B cell number   (MGI Ref ID J:133084)
      • increased transitional stage B cell number
        • T1 transitional B cells are insignificantly elevated   (MGI Ref ID J:133084)
        • increased T1 transitional B cells in inguinal lymph nodes   (MGI Ref ID J:133084)
  • abnormal lymphocyte physiology
    • survival of mature resting T and B cells in the absence of cytokines is improved compared to wild-type cells   (MGI Ref ID J:73316)
    • decreased B cell apoptosis
      • about 10-fold more B cells cultured without cytokines are alive after six days compared to controls   (MGI Ref ID J:73316)
    • decreased T cell apoptosis
      • double positive T cells with increased resistance to the apoptotic effects of dexamethasone   (MGI Ref ID J:133084)
      • about 10-fold more T cells cultured without cytokines are alive after six days compared to controls   (MGI Ref ID J:73316)
  • increased leukocyte cell number
    • increased white blood cells numbers (>3 fold)   (MGI Ref ID J:153846)
    • increased NK cell number
      • mice have a higher percent of KLRG1+CD27- NK cells in the spleen, liver and bone marrow compared to wild-type mice   (MGI Ref ID J:123409)
    • increased immature B cell number   (MGI Ref ID J:133084)
      • increased transitional stage B cell number
        • T1 transitional B cells are insignificantly elevated   (MGI Ref ID J:133084)
        • increased T1 transitional B cells in inguinal lymph nodes   (MGI Ref ID J:133084)
    • increased mature B cell number   (MGI Ref ID J:133084)
    • increased regulatory T cell number
  • increased spleen weight
    • increased spleen weight (>2.5 fold)   (MGI Ref ID J:153846)

Bcl2l11tm1.1Ast/Bcl2l11tm1.1Ast

        B6.129-Bcl2l11tm1.1Ast/J
  • hematopoietic system phenotype
  • decreased lymphocyte cell number   (MGI Ref ID J:137609)
  • immune system phenotype
  • decreased lymphocyte cell number   (MGI Ref ID J:137609)

Bcl2l11tm1.1Ast/Bcl2l11tm1.1Ast

        B6.129S1-Bcl2l11tm1.1Ast/J
  • immune system phenotype
  • decreased T cell apoptosis
    • increased viability of CD4 and CD8 T cells during in vitro culture of T cells purified from spleen and lymph nodes   (MGI Ref ID J:153652)
  • increased B cell number
    • a large increase in the number of B cells in the spleen   (MGI Ref ID J:153652)
  • increased CD4-positive T cell number
    • a large increase in the number of CD4-positive T cells in the spleen   (MGI Ref ID J:153652)
  • increased CD8-positive T cell number
    • a large increase in the number of CD8-positive T cells in the spleen   (MGI Ref ID J:153652)
  • increased splenocyte number
    • increased number of splenocytes (>3 fold)   (MGI Ref ID J:153652)
  • hematopoietic system phenotype
  • decreased T cell apoptosis
    • increased viability of CD4 and CD8 T cells during in vitro culture of T cells purified from spleen and lymph nodes   (MGI Ref ID J:153652)
  • increased B cell number
    • a large increase in the number of B cells in the spleen   (MGI Ref ID J:153652)
  • increased CD4-positive T cell number
    • a large increase in the number of CD4-positive T cells in the spleen   (MGI Ref ID J:153652)
  • increased CD8-positive T cell number
    • a large increase in the number of CD8-positive T cells in the spleen   (MGI Ref ID J:153652)
  • increased splenocyte number
    • increased number of splenocytes (>3 fold)   (MGI Ref ID J:153652)
  • cellular phenotype
  • decreased T cell apoptosis
    • increased viability of CD4 and CD8 T cells during in vitro culture of T cells purified from spleen and lymph nodes   (MGI Ref ID J:153652)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Bcl2l11tm1.1Ast/Bcl2l11+

        involves: 129S1/Sv * C57BL/6
  • mortality/aging
  • premature death
    • by 1 year of age 35% of heterozygotes are terminally ill   (MGI Ref ID J:58641)
  • immune system phenotype
  • abnormal lymphocyte physiology
    • pre-T cells cultured without cytokines or with ionomycin or taxol survive better than wild-type cells but not as well as homozygous cells   (MGI Ref ID J:58641)
    • increased IgA level
      • increased at 6 to 12 months of age but less than in homozygous mice   (MGI Ref ID J:58641)
    • increased IgG level
      • increased at 6 to 12 months of age but less than in homozygous mice   (MGI Ref ID J:58641)
    • increased IgM level
      • increased at 6 to 12 months of age but less than in homozygous mice   (MGI Ref ID J:58641)
  • glomerulonephritis
    • by 1 year of about 85% of terminally ill heterozygotes have immune complex glomerulonephritis   (MGI Ref ID J:58641)
  • increased susceptibility to autoimmune disorder
    • older heterozygotes develop lymphadenopathy   (MGI Ref ID J:58641)
  • vasculitis
    • about 20% of terminally ill heterozygotes show signs of vasculitis or cardiac infarction   (MGI Ref ID J:58641)
  • renal/urinary system phenotype
  • glomerulonephritis
    • by 1 year of about 85% of terminally ill heterozygotes have immune complex glomerulonephritis   (MGI Ref ID J:58641)
  • cardiovascular system phenotype
  • vasculitis
    • about 20% of terminally ill heterozygotes show signs of vasculitis or cardiac infarction   (MGI Ref ID J:58641)
  • hematopoietic system phenotype
  • abnormal lymphocyte physiology
    • pre-T cells cultured without cytokines or with ionomycin or taxol survive better than wild-type cells but not as well as homozygous cells   (MGI Ref ID J:58641)
    • increased IgA level
      • increased at 6 to 12 months of age but less than in homozygous mice   (MGI Ref ID J:58641)
    • increased IgG level
      • increased at 6 to 12 months of age but less than in homozygous mice   (MGI Ref ID J:58641)
    • increased IgM level
      • increased at 6 to 12 months of age but less than in homozygous mice   (MGI Ref ID J:58641)

Bcl2l11tm1.1Ast/Bcl2l11tm1.1Ast

        involves: 129S1/Sv * C57BL/6
  • mortality/aging
  • partial embryonic lethality during organogenesis
    • about half die before E10; however, this percentage is highly variable and background dependent   (MGI Ref ID J:58641)
  • premature death
    • by 1 year of age 55% of homozygotes are terminally ill with 85% of these developing kidney disease (immune complex glomerulonephritis)   (MGI Ref ID J:58641)
  • immune system phenotype
  • abnormal lymphocyte physiology
    • pre-T cells cultured without cytokines or with ionomycin or taxol survive 10 to 30 times better than wild-type cells   (MGI Ref ID J:58641)
    • pre-T cells are resistant to dexamethasone or gamma-irradiation induced cell death but not to phorbol 12-myristate 13-acetate (PMA) or Fas ligand induced cell death   (MGI Ref ID J:58641)
    • pre-B cells are also resistant to cell death induced by cytokine withdrawal, ionomycin, taxol, and dexamethasone but display sensitivity to cell death similar to wild-type following gamma irradiation or exposure to PMA or Fas ligand   (MGI Ref ID J:58641)
    • abnormal B cell proliferation
      • anti-CD40 B cell proliferation is increased 3- to 5- fold in the presence of IL-21, which does not occur in wild-type controls   (MGI Ref ID J:90963)
    • decreased B cell apoptosis
      • IL-21 fails to induce apoptosis in LPS-activated B cells as it does in wild-type mice   (MGI Ref ID J:90963)
    • increased IgA level
      • elevated about 3-fold at 6 to 12 months of age   (MGI Ref ID J:58641)
    • increased IgG level
      • elevated about 10-fold at 6 to 12 months of age   (MGI Ref ID J:58641)
    • increased IgM level
      • elevated about 3-fold at 6 to 12 months of age   (MGI Ref ID J:58641)
  • abnormal plasma cell morphology
    • at 6 to 12 months of age the number of IgM and IgG is increased by about 4-fold and 30- to 200-fold, respectively   (MGI Ref ID J:58641)
  • abnormal spleen morphology   (MGI Ref ID J:58641)
    • abnormal splenic cell ratio
      • T1:follicular B cell ratio is higher than wild-type   (MGI Ref ID J:132217)
    • enlarged spleen
      • in older homozygotes spleen size is increased 5- to 10-fold   (MGI Ref ID J:58641)
      • spleen hyperplasia
        • seen in older homozygotes with plasma cells most severely affected   (MGI Ref ID J:58641)
    • increased splenocyte number
      • total number of CD19+ splenocytes is higher than wild-type   (MGI Ref ID J:132217)
      • total number of splenocytes is increased relative to wild-type   (MGI Ref ID J:132217)
  • decreased double-positive T cell number
    • in adult mice, thymic pre-T cells are reduced by about 50% compared to wild-type; however in newborns thymocyte composition is similar to wild-type   (MGI Ref ID J:58641)
  • increased autoantibody level
    • total anti-IgM antibody levels are increased compared to wild-type   (MGI Ref ID J:132217)
    • increased anti-nuclear antigen antibody level
      • anti-nuclear antibodies are increased compared to wild-type   (MGI Ref ID J:132217)
      • increased anti-double stranded DNA antibody level
        • anti-SsDNA IgM and IgG antibodies are increased compared to wild-type   (MGI Ref ID J:132217)
      • increased anti-histone antibody level
        • increased compared to wild-type   (MGI Ref ID J:132217)
      • increased anti-single stranded DNA antibody level
        • anti-ssDNA IgM and IgG antibodies are increased compared to wild-type   (MGI Ref ID J:132217)
  • increased inflammatory response   (MGI Ref ID J:58641)
    • glomerulonephritis
      • by 1 year of about 85% of terminally ill homozygotes have immune complex glomerulonephritis   (MGI Ref ID J:58641)
    • vasculitis
      • about 20% of terminally ill homozygotes show signs of vasculitis or cardiac infarction   (MGI Ref ID J:58641)
  • increased leukocyte cell number
    • blood, spleen, and lymph node leukocyte numbers are elevated several fold mostly as a result of increased B and T cell counts   (MGI Ref ID J:58641)
    • these cells are mostly small, noncycling cells suggesting an increase in cell survival rather than proliferation   (MGI Ref ID J:58641)
    • however, the number of red cells is normal and the number of hematopoietic progenitor cells in the bone marrow is similar to wild-type   (MGI Ref ID J:58641)
    • increased B cell number
      • 2- to 4-fold increase   (MGI Ref ID J:58641)
      • increased follicular B cell number
        • higher in spleen relative to wild-type and Faslpr homozygotes   (MGI Ref ID J:132217)
      • increased immature B cell number
        • plasmablast numbers in spleen are increased relative to wild-type   (MGI Ref ID J:132217)
        • increased transitional stage B cell number
          • higher numbers of T1 and T2 B cells in spleen relative to wild-type   (MGI Ref ID J:132217)
      • increased marginal zone B cell number
        • higher in spleen relative to wild-type and Faslpr homozygotes   (MGI Ref ID J:132217)
    • increased T cell number
      • 2- to 4-fold increase involving mostly mature T cells is seen in adult mice   (MGI Ref ID J:58641)
      • increased CD4-positive T cell number
        • 2- to 3-fold increase in the number of CD4+ T cells is seen in adult mice   (MGI Ref ID J:58641)
      • increased CD8-positive T cell number
        • 2- to 3-fold increase in the number of CD8+ T cells is seen in adult mice   (MGI Ref ID J:58641)
      • increased double-negative T cell number
        • 2- to 3-fold increase is seen in adult mice   (MGI Ref ID J:58641)
    • increased granulocyte number
      • 2- to 4-fold increase   (MGI Ref ID J:58641)
    • increased monocyte cell number
      • 2- to 4-fold increase   (MGI Ref ID J:58641)
  • increased susceptibility to autoimmune disorder
    • older homozygotes develop lymphadenopathy   (MGI Ref ID J:58641)
  • renal/urinary system phenotype
  • abnormal kidney morphology
    • number of macrophages surrounding glomeruli is increased compared to wild-type and Faslpr homozygotes   (MGI Ref ID J:132217)
    • number of macrophages surrounding glomeruli is increased compared to wild-type, and is similar to that in double mutants   (MGI Ref ID J:132217)
    • abnormal renal glomerulus basement membrane morphology
      • IgG deposits mainly localized to glomerular basement membrane are increased relative to wild-type   (MGI Ref ID J:132217)
    • abnormal renal glomerulus morphology
      • hypercellularity is seen in homozygotes with kidney disease   (MGI Ref ID J:58641)
      • expanded mesangial matrix   (MGI Ref ID J:58641)
      • glomerular crescent
        • proliferation of capsular epithelial cells is seen in homozygotes with kidney disease   (MGI Ref ID J:58641)
      • glomerulonephritis
        • by 1 year of about 85% of terminally ill homozygotes have immune complex glomerulonephritis   (MGI Ref ID J:58641)
    • dilated renal tubules
      • eosinophilic deposits are seen in dilated renal tubules in homozygotes with kidney disease   (MGI Ref ID J:58641)
    • renal cast
      • dilated renal tubules contain eosinophilic casts   (MGI Ref ID J:58641)
  • increased renal glomerulus apoptosis
    • higher numbers of apoptotic cells are detected in glomeruli compared to wild-type   (MGI Ref ID J:132217)
  • homeostasis/metabolism phenotype
  • increased blood urea nitrogen level   (MGI Ref ID J:58641)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • unlike mice homozygous for Bbc3tm1Ast neuron sensitivity to oxidative stressor induced apoptosis is similar to controls   (MGI Ref ID J:127640)
  • hematopoietic system phenotype
  • abnormal lymphocyte physiology
    • pre-T cells cultured without cytokines or with ionomycin or taxol survive 10 to 30 times better than wild-type cells   (MGI Ref ID J:58641)
    • pre-T cells are resistant to dexamethasone or gamma-irradiation induced cell death but not to phorbol 12-myristate 13-acetate (PMA) or Fas ligand induced cell death   (MGI Ref ID J:58641)
    • pre-B cells are also resistant to cell death induced by cytokine withdrawal, ionomycin, taxol, and dexamethasone but display sensitivity to cell death similar to wild-type following gamma irradiation or exposure to PMA or Fas ligand   (MGI Ref ID J:58641)
    • abnormal B cell proliferation
      • anti-CD40 B cell proliferation is increased 3- to 5- fold in the presence of IL-21, which does not occur in wild-type controls   (MGI Ref ID J:90963)
    • decreased B cell apoptosis
      • IL-21 fails to induce apoptosis in LPS-activated B cells as it does in wild-type mice   (MGI Ref ID J:90963)
    • increased IgA level
      • elevated about 3-fold at 6 to 12 months of age   (MGI Ref ID J:58641)
    • increased IgG level
      • elevated about 10-fold at 6 to 12 months of age   (MGI Ref ID J:58641)
    • increased IgM level
      • elevated about 3-fold at 6 to 12 months of age   (MGI Ref ID J:58641)
  • abnormal plasma cell morphology
    • at 6 to 12 months of age the number of IgM and IgG is increased by about 4-fold and 30- to 200-fold, respectively   (MGI Ref ID J:58641)
  • abnormal spleen morphology   (MGI Ref ID J:58641)
    • abnormal splenic cell ratio
      • T1:follicular B cell ratio is higher than wild-type   (MGI Ref ID J:132217)
    • enlarged spleen
      • in older homozygotes spleen size is increased 5- to 10-fold   (MGI Ref ID J:58641)
      • spleen hyperplasia
        • seen in older homozygotes with plasma cells most severely affected   (MGI Ref ID J:58641)
    • increased splenocyte number
      • total number of CD19+ splenocytes is higher than wild-type   (MGI Ref ID J:132217)
      • total number of splenocytes is increased relative to wild-type   (MGI Ref ID J:132217)
  • decreased double-positive T cell number
    • in adult mice, thymic pre-T cells are reduced by about 50% compared to wild-type; however in newborns thymocyte composition is similar to wild-type   (MGI Ref ID J:58641)
  • decreased platelet cell number
    • platelet count is reduced to about 1/2 of wild-type; however, megakaryocyte numbers are normal   (MGI Ref ID J:58641)
  • increased leukocyte cell number
    • blood, spleen, and lymph node leukocyte numbers are elevated several fold mostly as a result of increased B and T cell counts   (MGI Ref ID J:58641)
    • these cells are mostly small, noncycling cells suggesting an increase in cell survival rather than proliferation   (MGI Ref ID J:58641)
    • however, the number of red cells is normal and the number of hematopoietic progenitor cells in the bone marrow is similar to wild-type   (MGI Ref ID J:58641)
    • increased B cell number
      • 2- to 4-fold increase   (MGI Ref ID J:58641)
      • increased follicular B cell number
        • higher in spleen relative to wild-type and Faslpr homozygotes   (MGI Ref ID J:132217)
      • increased immature B cell number
        • plasmablast numbers in spleen are increased relative to wild-type   (MGI Ref ID J:132217)
        • increased transitional stage B cell number
          • higher numbers of T1 and T2 B cells in spleen relative to wild-type   (MGI Ref ID J:132217)
      • increased marginal zone B cell number
        • higher in spleen relative to wild-type and Faslpr homozygotes   (MGI Ref ID J:132217)
    • increased T cell number
      • 2- to 4-fold increase involving mostly mature T cells is seen in adult mice   (MGI Ref ID J:58641)
      • increased CD4-positive T cell number
        • 2- to 3-fold increase in the number of CD4+ T cells is seen in adult mice   (MGI Ref ID J:58641)
      • increased CD8-positive T cell number
        • 2- to 3-fold increase in the number of CD8+ T cells is seen in adult mice   (MGI Ref ID J:58641)
      • increased double-negative T cell number
        • 2- to 3-fold increase is seen in adult mice   (MGI Ref ID J:58641)
    • increased granulocyte number
      • 2- to 4-fold increase   (MGI Ref ID J:58641)
    • increased monocyte cell number
      • 2- to 4-fold increase   (MGI Ref ID J:58641)
  • cardiovascular system phenotype
  • vasculitis
    • about 20% of terminally ill homozygotes show signs of vasculitis or cardiac infarction   (MGI Ref ID J:58641)
  • cellular phenotype
  • decreased B cell apoptosis
    • IL-21 fails to induce apoptosis in LPS-activated B cells as it does in wild-type mice   (MGI Ref ID J:90963)
  • increased renal glomerulus apoptosis
    • higher numbers of apoptotic cells are detected in glomeruli compared to wild-type   (MGI Ref ID J:132217)

Bcl2l11tm1.1Ast/Bcl2l11tm1.1Ast

        involves: 129S1/Sv
  • immune system phenotype
  • abnormal CD4-positive T cell number
    • 2- to 4- fold increase   (MGI Ref ID J:132819)
  • abnormal CD8-positive T cell physiology
    • HSV-1 antigen specific CD8+ T cells show nearly complete resistance to cytokine withdrawal induced apoptosis in culture   (MGI Ref ID J:132819)
  • abnormal splenic cell ratio
    • increase in the number of HSV-1 specific T cells in the spleen but not lymph nodes after infection   (MGI Ref ID J:132819)
  • increased B cell number
    • 2- to 4- fold increase in B220+ positive cells   (MGI Ref ID J:132819)
  • hematopoietic system phenotype
  • *normal* hematopoietic system phenotype
    • unlike in Bnip3ltm1Ney homozygotes, reticulocytes clearance of mitochondria is normal   (MGI Ref ID J:141545)
    • abnormal CD4-positive T cell number
      • 2- to 4- fold increase   (MGI Ref ID J:132819)
    • abnormal CD8-positive T cell physiology
      • HSV-1 antigen specific CD8+ T cells show nearly complete resistance to cytokine withdrawal induced apoptosis in culture   (MGI Ref ID J:132819)
    • abnormal splenic cell ratio
      • increase in the number of HSV-1 specific T cells in the spleen but not lymph nodes after infection   (MGI Ref ID J:132819)
    • increased B cell number
      • 2- to 4- fold increase in B220+ positive cells   (MGI Ref ID J:132819)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Bcl2l11tm1.1Ast related

Apoptosis Research
Endogenous Regulators

Cancer Research
Genes Regulating Growth and Proliferation

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
Intracellular Signaling Molecules

Internal/Organ Research
Kidney Defects
      polycystic kidney disease
Spleen Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Bcl2l11tm1.1Ast
Allele Name targeted mutation 1.1, Andreas Strasser
Allele Type Targeted (Null/Knockout)
Common Name(s) bim-;
Mutation Made By Andreas Strasser,   Walter and Eliza Hall Inst of Med Res
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line NameW9.5/W95
ES Cell Line Strain129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Bcl2l11, BCL2-like 11 (apoptosis facilitator)
Chromosome 2
Gene Common Name(s) 1500006F24Rik; BAM; BIM; BOD; Bcl2 interacting mediator of cell death; Bim; BimL; RIKEN cDNA 1500006F24 gene;
Molecular Note Insertion of a floxed PGK-neomycin cassette deleted the exon encoding the BH3 domain. Cre-mediated recombination in vivo subsequently removed the inserted cassette. In the final allele a single loxP site remains in place of the exon. Immunoblot analysis using a rat monoclonal antibody did not detect full-length protein, but did detect a small amount of a truncated polypeptide in spleen cells from homozygous mutant mice. [MGI Ref ID J:58641]

Genotyping

Genotyping Information

Genotyping Protocols

Bcl2l11tm1.1Ast, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Bouillet P; Metcalf D; Huang DC; Tarlinton DM; Kay TW; Kontgen F; Adams JM; Strasser A. 1999. Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity. Science 286(5445):1735-8. [PubMed: 10576740]  [MGI Ref ID J:58641]

Additional References

Akiyama T; Bouillet P; Miyazaki T; Kadono Y; Chikuda H; Chung UI; Fukuda A; Hikita A; Seto H; Okada T; Inaba T; Sanjay A; Baron R; Kawaguchi H; Oda H; Nakamura K; Strasser A; Tanaka S. 2003. Regulation of osteoclast apoptosis by ubiquitylation of proapoptotic BH3-only Bcl-2 family member Bim. EMBO J 22(24):6653-6664. [PubMed: 14657036]  [MGI Ref ID J:87072]

Bouillet P; Purton JF; Godfrey DI; Zhang LC; Coultas L; Puthalakath H; Pellegrini M; Cory S; Adams JM; Strasser A. 2002. BH3-only Bcl-2 family member Bim is required for apoptosis of autoreactive thymocytes. Nature 415(6874):922-6. [PubMed: 11859372]  [MGI Ref ID J:74991]

Bouillet P; Zhang LC; Huang DC; Webb GC; Bottema CD; Shore P; Eyre HJ; Sutherland GR; Adams JM. 2001. Gene structure alternative splicing, and chromosomal localization of pro-apoptotic Bcl-2 relative Bim. Mamm Genome 12(2):163-8. [PubMed: 11210187]  [MGI Ref ID J:69112]

Bcl2l11tm1.1Ast related

Akiyama T; Bouillet P; Miyazaki T; Kadono Y; Chikuda H; Chung UI; Fukuda A; Hikita A; Seto H; Okada T; Inaba T; Sanjay A; Baron R; Kawaguchi H; Oda H; Nakamura K; Strasser A; Tanaka S. 2003. Regulation of osteoclast apoptosis by ubiquitylation of proapoptotic BH3-only Bcl-2 family member Bim. EMBO J 22(24):6653-6664. [PubMed: 14657036]  [MGI Ref ID J:87072]

Alfredsson J; Puthalakath H; Martin H; Strasser A; Nilsson G. 2005. Proapoptotic Bcl-2 family member Bim is involved in the control of mast cell survival and is induced together with Bcl-XL upon IgE-receptor activation. Cell Death Differ 12(2):136-44. [PubMed: 15592435]  [MGI Ref ID J:106372]

Andina N; Conus S; Schneider EM; Fey MF; Simon HU. 2009. Induction of Bim limits cytokine-mediated prolonged survival of neutrophils. Cell Death Differ 16(9):1248-55. [PubMed: 19407828]  [MGI Ref ID J:164177]

Barnes MJ; Aksoylar H; Krebs P; Bourdeau T; Arnold CN; Xia Y; Khovananth K; Engel I; Sovath S; Lampe K; Laws E; Saunders A; Butcher GW; Kronenberg M; Steinbrecher K; Hildeman D; Grimes HL; Beutler B; Hoebe K. 2010. Loss of T cell and B cell quiescence precedes the onset of microbial flora-dependent wasting disease and intestinal inflammation in Gimap5-deficient mice. J Immunol 184(7):3743-54. [PubMed: 20190135]  [MGI Ref ID J:160090]

Barone MC; Desouza LA; Freeman RS. 2008. Pin1 promotes cell death in NGF-dependent neurons through a mechanism requiring c-Jun activity. J Neurochem 106(2):734-45. [PubMed: 18419764]  [MGI Ref ID J:139389]

Bauer A; Kirschnek S; Hacker G. 2007. Inhibition of apoptosis can be accompanied by increased Bim levels in T lymphocytes and neutrophil granulocytes. Cell Death Differ 14(9):1714-6. [PubMed: 17585338]  [MGI Ref ID J:141495]

Bauer A; Villunger A; Labi V; Fischer SF; Strasser A; Wagner H; Schmid RM; Hacker G. 2006. The NF-kappaB regulator Bcl-3 and the BH3-only proteins Bim and Puma control the death of activated T cells. Proc Natl Acad Sci U S A 103(29):10979-84. [PubMed: 16832056]  [MGI Ref ID J:111822]

Bohgaki T; Mozo J; Salmena L; Matysiak-Zablocki E; Bohgaki M; Sanchez O; Strasser A; Hakem A; Hakem R. 2011. Caspase-8 inactivation in T cells increases necroptosis and suppresses autoimmunity in Bim-/- mice. J Cell Biol 195(2):277-91. [PubMed: 22006951]  [MGI Ref ID J:177715]

Bouillet P; Cory S; Zhang LC; Strasser A; Adams JM. 2001. Degenerative Disorders Caused by Bcl-2 Deficiency Prevented by Loss of Its BH3-Only Antagonist Bim. Dev Cell 1(5):645-53. [PubMed: 11709185]  [MGI Ref ID J:73316]

Bouillet P; Robati M; Bath M; Strasser A. 2005. Polycystic kidney disease prevented by transgenic RNA interference. Cell Death Differ 12(7):831-3. [PubMed: 15818405]  [MGI Ref ID J:128559]

Bradfute SB; Swanson PE; Smith MA; Watanabe E; McDunn JE; Hotchkiss RS; Bavari S. 2010. Mechanisms and consequences of ebolavirus-induced lymphocyte apoptosis. J Immunol 184(1):327-35. [PubMed: 20028660]  [MGI Ref ID J:158994]

Busuttil V; Droin N; McCormick L; Bernassola F; Candi E; Melino G; Green DR. 2010. NF-kappaB inhibits T-cell activation-induced, p73-dependent cell death by induction of MDM2. Proc Natl Acad Sci U S A 107(42):18061-6. [PubMed: 20921405]  [MGI Ref ID J:165536]

Campbell KJ; Bath ML; Turner ML; Vandenberg CJ; Bouillet P; Metcalf D; Scott CL; Cory S. 2010. Elevated Mcl-1 perturbs lymphopoiesis, promotes transformation of hematopoietic stem/progenitor cells, and enhances drug resistance. Blood 116(17):3197-207. [PubMed: 20631380]  [MGI Ref ID J:165867]

Cante-Barrett K; Winslow MM; Crabtree GR. 2007. Selective role of NFATc3 in positive selection of thymocytes. J Immunol 179(1):103-10. [PubMed: 17579027]  [MGI Ref ID J:141917]

Chang KC; Unsinger J; Davis CG; Schwulst SJ; Muenzer JT; Strasser A; Hotchkiss RS. 2007. Multiple triggers of cell death in sepsis: death receptor and mitochondrial-mediated apoptosis. FASEB J 21(3):708-19. [PubMed: 17307841]  [MGI Ref ID J:134855]

Chao JR; Parganas E; Boyd K; Hong CY; Opferman JT; Ihle JN. 2008. Hax1-mediated processing of HtrA2 by Parl allows survival of lymphocytes and neurons. Nature 452(7183):98-102. [PubMed: 18288109]  [MGI Ref ID J:132627]

Chino T; Draves KE; Clark EA. 2009. Regulation of dendritic cell survival and cytokine production by osteoprotegerin. J Leukoc Biol 86(4):933-40. [PubMed: 19641036]  [MGI Ref ID J:153427]

Chougnet CA; Tripathi P; Lages CS; Raynor J; Sholl A; Fink P; Plas DR; Hildeman DA. 2011. A major role for Bim in regulatory T cell homeostasis. J Immunol 186(1):156-63. [PubMed: 21098226]  [MGI Ref ID J:168007]

Cippa PE; Gabriel SS; Chen J; Bardwell PD; Bushell A; Guimezanes A; Kraus AK; Wekerle T; Wuthrich RP; Fehr T. 2013. Targeting apoptosis to induce stable mixed hematopoietic chimerism and long-term allograft survival without myelosuppressive conditioning in mice. Blood 122(9):1669-77. [PubMed: 23869083]  [MGI Ref ID J:202232]

Clybouw C; Merino D; Nebl T; Masson F; Robati M; O'Reilly L; Hubner A; Davis RJ; Strasser A; Bouillet P. 2012. Alternative splicing of Bim and Erk-mediated Bim(EL) phosphorylation are dispensable for hematopoietic homeostasis in vivo. Cell Death Differ 19(6):1060-8. [PubMed: 22240894]  [MGI Ref ID J:203181]

Concannon CG; Tuffy LP; Weisova P; Bonner HP; Davila D; Bonner C; Devocelle MC; Strasser A; Ward MW; Prehn JH. 2010. AMP kinase-mediated activation of the BH3-only protein Bim couples energy depletion to stress-induced apoptosis. J Cell Biol 189(1):83-94. [PubMed: 20351066]  [MGI Ref ID J:158799]

Corazza N; Jakob S; Schaer C; Frese S; Keogh A; Stroka D; Kassahn D; Torgler R; Mueller C; Schneider P; Brunner T. 2006. TRAIL receptor-mediated JNK activation and Bim phosphorylation critically regulate Fas-mediated liver damage and lethality. J Clin Invest 116(9):2493-9. [PubMed: 16955144]  [MGI Ref ID J:114519]

Coultas L; Bouillet P; Loveland KL; Meachem S; Perlman H; Adams JM; Strasser A. 2005. Concomitant loss of proapoptotic BH3-only Bcl-2 antagonists Bik and Bim arrests spermatogenesis. EMBO J 24(22):3963-73. [PubMed: 16270031]  [MGI Ref ID J:103605]

Coultas L; Terzano S; Thomas T; Voss A; Reid K; Stanley EG; Scott CL; Bouillet P; Bartlett P; Ham J; Adams JM; Strasser A. 2007. Hrk/DP5 contributes to the apoptosis of select neuronal populations but is dispensable for haematopoietic cell apoptosis. J Cell Sci 120(Pt 12):2044-52. [PubMed: 17535852]  [MGI Ref ID J:124044]

Craxton A; Draves KE; Clark EA. 2007. Bim regulates BCR-induced entry of B cells into the cell cycle. Eur J Immunol 37(10):2715-22. [PubMed: 17705137]  [MGI Ref ID J:125270]

Craxton A; Draves KE; Gruppi A; Clark EA. 2005. BAFF regulates B cell survival by downregulating the BH3-only family member Bim via the ERK pathway. J Exp Med 202(10):1363-74. [PubMed: 16301744]  [MGI Ref ID J:118846]

D'Souza WN; Chang CF; Fischer AM; Li M; Hedrick SM. 2008. The Erk2 MAPK regulates CD8 T cell proliferation and survival. J Immunol 181(11):7617-29. [PubMed: 19017950]  [MGI Ref ID J:142200]

Davila D; Connolly NM; Bonner H; Weisova P; Dussmann H; Concannon CG; Huber HJ; Prehn JH. 2012. Two-step activation of FOXO3 by AMPK generates a coherent feed-forward loop determining excitotoxic cell fate. Cell Death Differ 19(10):1677-88. [PubMed: 22539004]  [MGI Ref ID J:204794]

Doonan F; Donovan M; Gomez-Vicente V; Bouillet P; Cotter TG. 2007. Bim expression indicates the pathway to retinal cell death in development and degeneration. J Neurosci 27(40):10887-94. [PubMed: 17913922]  [MGI Ref ID J:125591]

Dunkle A; Dzhagalov I; He YW. 2010. Mcl-1 promotes survival of thymocytes by inhibition of Bak in a pathway separate from Bcl-2. Cell Death Differ 17(6):994-1002. [PubMed: 20057504]  [MGI Ref ID J:186374]

Edgar CE; Lindquist LD; McKean DL; Strasser A; Bram RJ. 2010. CAML regulates Bim-dependent thymocyte death. Cell Death Differ 17(10):1566-76. [PubMed: 20300112]  [MGI Ref ID J:186363]

Egle A; Harris AW; Bouillet P; Cory S. 2004. Bim is a suppressor of Myc-induced mouse B cell leukemia. Proc Natl Acad Sci U S A 101(16):6164-9. [PubMed: 15079075]  [MGI Ref ID J:89600]

Ekoff M; Kaufmann T; Engstrom M; Motoyama N; Villunger A; Jonsson JI; Strasser A; Nilsson G. 2007. The BH3-only protein Puma plays an essential role in cytokine deprivation induced apoptosis of mast cells. Blood 110(9):3209-17. [PubMed: 17634411]  [MGI Ref ID J:149129]

Enders A; Bouillet P; Puthalakath H; Xu Y; Tarlinton DM; Strasser A. 2003. Loss of the pro-apoptotic BH3-only Bcl-2 family member Bim inhibits BCR stimulation-induced apoptosis and deletion of autoreactive B cells. J Exp Med 198(7):1119-26. [PubMed: 14517273]  [MGI Ref ID J:86002]

Erlacher M; Labi V; Manzl C; Bock G; Tzankov A; Hacker G; Michalak E; Strasser A; Villunger A. 2006. Puma cooperates with Bim, the rate-limiting BH3-only protein in cell death during lymphocyte development, in apoptosis induction. J Exp Med 203(13):2939-51. [PubMed: 17178918]  [MGI Ref ID J:120274]

Erlacher M; Michalak EM; Kelly PN; Labi V; Niederegger H; Coultas L; Adams JM; Strasser A; Villunger A. 2005. BH3-only proteins Puma and Bim are rate-limiting for gamma-radiation- and glucocorticoid-induced apoptosis of lymphoid cells in vivo. Blood 106(13):4131-8. [PubMed: 16118324]  [MGI Ref ID J:124060]

Fassett MS; Jiang W; D'Alise AM; Mathis D; Benoist C. 2012. Nuclear receptor Nr4a1 modulates both regulatory T-cell (Treg) differentiation and clonal deletion. Proc Natl Acad Sci U S A 109(10):3891-6. [PubMed: 22345564]  [MGI Ref ID J:182146]

Fischer SF; Belz GT; Strasser A. 2008. BH3-only protein Puma contributes to death of antigen-specific T cells during shutdown of an immune response to acute viral infection. Proc Natl Acad Sci U S A 105(8):3035-40. [PubMed: 18287039]  [MGI Ref ID J:132819]

Fischer SF; Bouillet P; O'Donnell K; Light A; Tarlinton DM; Strasser A. 2007. Proapoptotic BH3-only protein Bim is essential for developmentally programmed death of germinal center-derived memory B cells and antibody-forming cells. Blood 110(12):3978-84. [PubMed: 17720882]  [MGI Ref ID J:149102]

Fortner KA; Bouillet P; Strasser A; Budd RC. 2010. Apoptosis regulators Fas and Bim synergistically control T-lymphocyte homeostatic proliferation. Eur J Immunol 40(11):3043-53. [PubMed: 21061436]  [MGI Ref ID J:165842]

Frenzel A; Labi V; Chmelewskij W; Ploner C; Geley S; Fiegl H; Tzankov A; Villunger A. 2010. Suppression of B-cell lymphomagenesis by the BH3-only proteins Bmf and Bad. Blood 115(5):995-1005. [PubMed: 19965635]  [MGI Ref ID J:156718]

Gallo EM; Winslow MM; Cante-Barrett K; Radermacher AN; Ho L; McGinnis L; Iritani B; Neilson JR; Crabtree GR. 2007. Calcineurin sets the bandwidth for discrimination of signals during thymocyte development. Nature 450(7170):731-5. [PubMed: 18046413]  [MGI Ref ID J:130376]

Garrison SP; Phillips DC; Jeffers JR; Chipuk JE; Parsons MJ; Rehg JE; Opferman JT; Green DR; Zambetti GP. 2012. Genetically defining the mechanism of Puma- and Bim-induced apoptosis. Cell Death Differ 19(4):642-9. [PubMed: 22015606]  [MGI Ref ID J:203075]

Gautier EL; Ivanov S; Lesnik P; Randolph GJ. 2013. Local apoptosis mediates clearance of macrophages from resolving inflammation in mice. Blood 122(15):2714-22. [PubMed: 23974197]  [MGI Ref ID J:203440]

Gavalda N; Perez-Navarro E; Garcia-Martinez JM; Marco S; Benito A; Alberch J. 2008. Bax deficiency promotes an up-regulation of Bim(EL) and Bak during striatal and cortical postnatal development, and after excitotoxic injury. Mol Cell Neurosci 37(4):663-72. [PubMed: 18272391]  [MGI Ref ID J:135298]

Ghosh AP; Klocke BJ; Ballestas ME; Roth KA. 2012. CHOP potentially co-operates with FOXO3a in neuronal cells to regulate PUMA and BIM expression in response to ER stress. PLoS One 7(6):e39586. [PubMed: 22761832]  [MGI Ref ID J:190239]

Goodyear CS; Corr M; Sugiyama F; Boyle DL; Silverman GJ. 2007. Cutting Edge: Bim is required for superantigen-mediated B cell death. J Immunol 178(5):2636-40. [PubMed: 17312102]  [MGI Ref ID J:144121]

Gray DH; Kupresanin F; Berzins SP; Herold MJ; O'Reilly LA; Bouillet P; Strasser A. 2012. The BH3-Only Proteins Bim and Puma Cooperate to Impose Deletional Tolerance of Organ-Specific Antigens. Immunity 37(3):451-62. [PubMed: 22960223]  [MGI Ref ID J:187669]

Gross AJ; Proekt I; DeFranco AL. 2011. Elevated BCR signaling and decreased survival of Lyn-deficient transitional and follicular B cells. Eur J Immunol 41(12):3645-55. [PubMed: 21928281]  [MGI Ref ID J:179511]

Hale JS; Nelson LT; Simmons KB; Fink PJ. 2011. Bcl-2-interacting mediator of cell death influences autoantigen-driven deletion and TCR revision. J Immunol 186(2):799-806. [PubMed: 21148799]  [MGI Ref ID J:168782]

Harder JM; Fernandes KA; Libby RT. 2012. The Bcl-2 family member BIM has multiple glaucoma-relevant functions in DBA/2J mice. Sci Rep 2:530. [PubMed: 22833783]  [MGI Ref ID J:207269]

Harder JM; Libby RT. 2011. BBC3 (PUMA) regulates developmental apoptosis but not axonal injury induced death in the retina. Mol Neurodegener 6:50. [PubMed: 21762490]  [MGI Ref ID J:207282]

Harder JM; Libby RT. 2013. Deficiency in Bim, Bid and Bbc3 (Puma) do not prevent axonal injury induced death. Cell Death Differ 20(1):182. [PubMed: 22996683]  [MGI Ref ID J:205628]

He S; Wang J; Kato K; Xie F; Varambally S; Mineishi S; Kuick R; Mochizuki K; Liu Y; Nieves E; Mani RS; Chinnaiyan AM; Marquez VE; Zhang Y. 2012. Inhibition of histone methylation arrests ongoing graft-versus-host disease in mice by selectively inducing apoptosis of alloreactive effector T cells. Blood 119(5):1274-82. [PubMed: 22117046]  [MGI Ref ID J:181783]

Henao-Mejia J; Williams A; Goff LA; Staron M; Licona-Limon P; Kaech SM; Nakayama M; Rinn JL; Flavell RA. 2013. The microRNA miR-181 is a critical cellular metabolic rheostat essential for NKT cell ontogenesis and lymphocyte development and homeostasis. Immunity 38(5):984-97. [PubMed: 23623381]  [MGI Ref ID J:203157]

Hu Q; Sader A; Parkman JC; Baldwin TA. 2009. Bim-mediated apoptosis is not necessary for thymic negative selection to ubiquitous self-antigens. J Immunol 183(12):7761-7. [PubMed: 19933852]  [MGI Ref ID J:157488]

Hubner A; Barrett T; Flavell RA; Davis RJ. 2008. Multisite phosphorylation regulates Bim stability and apoptotic activity. Mol Cell 30(4):415-25. [PubMed: 18498746]  [MGI Ref ID J:137061]

Hubner A; Cavanagh-Kyros J; Rincon M; Flavell RA; Davis RJ. 2009. Functional co-operation of the pro-apoptotic Bcl2-family proteins Bmf and Bim in vivo. Mol Cell Biol :. [PubMed: 19841067]  [MGI Ref ID J:153652]

Hughes P; Robati M; Lu W; Zhou J; Strasser A; Bouillet P. 2006. Loss of PKD1 and loss of Bcl-2 elicit polycystic kidney disease through distinct mechanisms. Cell Death Differ 13(7):1123-7. [PubMed: 16282979]  [MGI Ref ID J:126158]

Hughes PD; Belz GT; Fortner KA; Budd RC; Strasser A; Bouillet P. 2008. Apoptosis regulators Fas and Bim cooperate in shutdown of chronic immune responses and prevention of autoimmunity. Immunity 28(2):197-205. [PubMed: 18275830]  [MGI Ref ID J:132218]

Huntington ND; Labi V; Cumano A; Vieira P; Strasser A; Villunger A; Di Santo JP; Alves NL. 2009. Loss of the pro-apoptotic BH3-only Bcl-2 family member Bim sustains B lymphopoiesis in the absence of IL-7. Int Immunol 21(6):715-25. [PubMed: 19454543]  [MGI Ref ID J:149442]

Huntington ND; Puthalakath H; Gunn P; Naik E; Michalak EM; Smyth MJ; Tabarias H; Degli-Esposti MA; Dewson G; Willis SN; Motoyama N; Huang DC; Nutt SL; Tarlinton DM; Strasser A. 2007. Interleukin 15-mediated survival of natural killer cells is determined by interactions among Bim, Noxa and Mcl-1. Nat Immunol 8(8):856-863. [PubMed: 17618288]  [MGI Ref ID J:123409]

Hutcheson J; Perlman H. 2007. Loss of Bim results in abnormal accumulation of mature CD4-CD8-CD44-CD25- thymocytes. Immunobiology 212(8):629-36. [PubMed: 17869640]  [MGI Ref ID J:129934]

Hutcheson J; Scatizzi JC; Bickel E; Brown NJ; Bouillet P; Strasser A; Perlman H. 2005. Combined loss of proapoptotic genes Bak or Bax with Bim synergizes to cause defects in hematopoiesis and in thymocyte apoptosis. J Exp Med 201(12):1949-60. [PubMed: 15967824]  [MGI Ref ID J:99285]

Hutcheson J; Scatizzi JC; Siddiqui AM; Haines GK 3rd; Wu T; Li QZ; Davis LS; Mohan C; Perlman H. 2008. Combined deficiency of proapoptotic regulators Bim and Fas results in the early onset of systemic autoimmunity. Immunity 28(2):206-17. [PubMed: 18275831]  [MGI Ref ID J:132217]

Jabbour AM; Heraud JE; Daunt CP; Kaufmann T; Sandow J; O'Reilly LA; Callus BA; Lopez A; Strasser A; Vaux DL; Ekert PG. 2009. Puma indirectly activates Bax to cause apoptosis in the absence of Bid or Bim. Cell Death Differ 16(4):555-63. [PubMed: 19079139]  [MGI Ref ID J:158076]

Jin H; Carrio R; Yu A; Malek TR. 2004. Distinct activation signals determine whether IL-21 induces B cell costimulation, growth arrest, or Bim-dependent apoptosis. J Immunol 173(1):657-65. [PubMed: 15210829]  [MGI Ref ID J:90963]

Jones-Mason ME; Zhao X; Kappes D; Lasorella A; Iavarone A; Zhuang Y. 2012. E protein transcription factors are required for the development of CD4(+) lineage T cells. Immunity 36(3):348-61. [PubMed: 22425249]  [MGI Ref ID J:187334]

Jurado S; Gleeson K; O'Donnell K; Izon DJ; Walkley CR; Strasser A; Tarlinton DM; Heierhorst J. 2012. The Zinc-finger protein ASCIZ regulates B cell development via DYNLL1 and Bim. J Exp Med 209(9):1629-39. [PubMed: 22891272]  [MGI Ref ID J:191832]

Kaufmann T; Jost PJ; Pellegrini M; Puthalakath H; Gugasyan R; Gerondakis S; Cretney E; Smyth MJ; Silke J; Hakem R; Bouillet P; Mak TW; Dixit VM; Strasser A. 2009. Fatal hepatitis mediated by tumor necrosis factor TNFalpha requires caspase-8 and involves the BH3-only proteins Bid and Bim. Immunity 30(1):56-66. [PubMed: 19119023]  [MGI Ref ID J:143730]

Kelly PN; White MJ; Goschnick MW; Fairfax KA; Tarlinton DM; Kinkel SA; Bouillet P; Adams JM; Kile BT; Strasser A. 2010. Individual and overlapping roles of BH3-only proteins Bim and Bad in apoptosis of lymphocytes and platelets and in suppression of thymic lymphoma development. Cell Death Differ 17(10):1655-64. [PubMed: 20431598]  [MGI Ref ID J:186358]

Kerr JB; Hutt KJ; Michalak EM; Cook M; Vandenberg CJ; Liew SH; Bouillet P; Mills A; Scott CL; Findlay JK; Strasser A. 2012. DNA damage-induced primordial follicle oocyte apoptosis and loss of fertility require TAp63-mediated induction of Puma and Noxa. Mol Cell 48(3):343-52. [PubMed: 23000175]  [MGI Ref ID J:191005]

Kiss I; Oskolas H; Toth R; Bouillet P; Toth K; Fulop A; Scholtz B; Ledent C; Fesus L; Szondy Z. 2006. Adenosine A2A receptor-mediated cell death of mouse thymocytes involves adenylate cyclase and Bim and is negatively regulated by Nur77. Eur J Immunol 36(6):1559-71. [PubMed: 16673448]  [MGI Ref ID J:115069]

Kittipatarin C; Li W; Durum SK; Khaled AR. 2010. Cdc25A-driven proliferation regulates CD62L levels and lymphocyte movement in response to interleukin-7. Exp Hematol 38(12):1143-56. [PubMed: 20831893]  [MGI Ref ID J:170214]

Knell J; Best JA; Lind NA; Yang E; D'Cruz LM; Goldrath AW. 2013. Id2 Influences Differentiation of Killer Cell Lectin-like Receptor G1hi Short-Lived CD8+ Effector T Cells. J Immunol 190(4):1501-9. [PubMed: 23325888]  [MGI Ref ID J:193405]

Koralov SB; Muljo SA; Galler GR; Krek A; Chakraborty T; Kanellopoulou C; Jensen K; Cobb BS; Merkenschlager M; Rajewsky N; Rajewsky K. 2008. Dicer ablation affects antibody diversity and cell survival in the B lymphocyte lineage. Cell 132(5):860-74. [PubMed: 18329371]  [MGI Ref ID J:135783]

Koulnis M; Porpiglia E; Porpiglia PA; Liu Y; Hallstrom K; Hidalgo D; Socolovsky M. 2012. Contrasting dynamic responses in vivo of the Bcl-xL and Bim erythropoietic survival pathways. Blood 119(5):1228-39. [PubMed: 22086418]  [MGI Ref ID J:181805]

Labi V; Erlacher M; Kiessling S; Manzl C; Frenzel A; O'Reilly L; Strasser A; Villunger A. 2008. Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development. J Exp Med 205(3):641-55. [PubMed: 18299399]  [MGI Ref ID J:133084]

Labi V; Woess C; Tuzlak S; Erlacher M; Bouillet P; Strasser A; Tzankov A; Villunger A. 2014. Deregulated cell death and lymphocyte homeostasis cause premature lethality in mice lacking the BH3-only proteins Bim and Bmf. Blood 123(17):2652-62. [PubMed: 24632712]  [MGI Ref ID J:210878]

Lalanne AI; Moraga I; Hao Y; Pereira JP; Alves NL; Huntington ND; Freitas AA; Cumano A; Vieira P. 2010. CpG inhibits pro-B cell expansion through a cathepsin B-dependent mechanism. J Immunol 184(10):5678-85. [PubMed: 20400700]  [MGI Ref ID J:160995]

Lauer C; Brunner T; Corazza N. 2012. The proapoptotic Bcl-2 family member Bim plays a central role during the development of virus-induced hepatitis. J Immunol 188(2):916-22. [PubMed: 22156338]  [MGI Ref ID J:180886]

Leverrier S; Salvesen GS; Walsh CM. 2011. Enzymatically active single chain caspase-8 maintains T-cell survival during clonal expansion. Cell Death Differ 18(1):90-8. [PubMed: 20523353]  [MGI Ref ID J:186349]

Li WQ; Guszczynski T; Hixon JA; Durum SK. 2010. Interleukin-7 regulates Bim proapoptotic activity in peripheral T-cell survival. Mol Cell Biol 30(3):590-600. [PubMed: 19933849]  [MGI Ref ID J:156385]

Lin KR; Li CL; Yen JJ; Yang-Yen HF. 2013. Constitutive phosphorylation of GATA-1 at serine(2)(6) attenuates the colony-forming activity of erythrocyte-committed progenitors. PLoS One 8(5):e64269. [PubMed: 23717580]  [MGI Ref ID J:200716]

Lindemann RK; Newbold A; Whitecross KF; Cluse LA; Frew AJ; Ellis L; Williams S; Wiegmans AP; Dear AE; Scott CL; Pellegrini M; Wei A; Richon VM; Marks PA; Lowe SW; Smyth MJ; Johnstone RW. 2007. Analysis of the apoptotic and therapeutic activities of histone deacetylase inhibitors by using a mouse model of B cell lymphoma. Proc Natl Acad Sci U S A 104(19):8071-6. [PubMed: 17470784]  [MGI Ref ID J:121589]

Ludwinski MW; Sun J; Hilliard B; Gong S; Xue F; Carmody RJ; DeVirgiliis J; Chen YH. 2009. Critical roles of Bim in T cell activation and T cell-mediated autoimmune inflammation in mice. J Clin Invest 119(6):1706-13. [PubMed: 19411758]  [MGI Ref ID J:150453]

Maeda T; Ito K; Merghoub T; Poliseno L; Hobbs RM; Wang G; Dong L; Maeda M; Dore LC; Zelent A; Luzzatto L; Teruya-Feldstein J; Weiss MJ; Pandolfi PP. 2009. LRF is an essential downstream target of GATA1 in erythroid development and regulates BIM-dependent apoptosis. Dev Cell 17(4):527-40. [PubMed: 19853566]  [MGI Ref ID J:154780]

Man K; Miasari M; Shi W; Xin A; Henstridge DC; Preston S; Pellegrini M; Belz GT; Smyth GK; Febbraio MA; Nutt SL; Kallies A. 2013. The transcription factor IRF4 is essential for TCR affinity-mediated metabolic programming and clonal expansion of T cells. Nat Immunol 14(11):1155-65. [PubMed: 24056747]  [MGI Ref ID J:208205]

Marechal Y; Pesesse X; Jia Y; Pouillon V; Perez-Morga D; Daniel J; Izui S; Cullen PJ; Leo O; Luo HR; Erneux C; Schurmans S. 2007. Inositol 1,3,4,5-tetrakisphosphate controls proapoptotic Bim gene expression and survival in B cells. Proc Natl Acad Sci U S A 104(35):13978-83. [PubMed: 17709751]  [MGI Ref ID J:124904]

Marsden VS; O'Connor L; O'Reilly LA; Silke J; Metcalf D; Ekert PG; Huang DC; Cecconi F; Kuida K; Tomaselli KJ; Roy S; Nicholson DW; Vaux DL; Bouillet P; Adams JM; Strasser A. 2002. Apoptosis initiated by Bcl-2-regulated caspase activation independently of the cytochrome c/Apaf-1/caspase-9 apoptosome. Nature 419(6907):634-7. [PubMed: 12374983]  [MGI Ref ID J:131246]

Masson F; Kupresanin F; Mount A; Strasser A; Belz GT. 2011. Bid and Bim Collaborate during Induction of T Cell Death in Persistent Infection. J Immunol 186(7):4059-66. [PubMed: 21339359]  [MGI Ref ID J:170848]

Melum E; Franke A; Schramm C; Weismuller TJ; Gotthardt DN; Offner FA; Juran BD; Laerdahl JK; Labi V; Bjornsson E; Weersma RK; Henckaerts L; Teufel A; Rust C; Ellinghaus E; Balschun T; Boberg KM; Ellinghaus D; Bergquist A; Sauer P; Ryu E; Hov JR; Wedemeyer J; Lindkvist B; Wittig M; Porte RJ; Holm K; Gieger C; Wichmann HE; Stokkers P; Ponsioen CY; Runz H; Stiehl A; Wijmenga C; Sterneck M; Vermeire S; Beuers U; Villunger A; Schrumpf E; Lazaridis KN; Manns MP; Schreiber S; Karlsen TH. 2011. Genome-wide association analysis in primary sclerosing cholangitis identifies two non-HLA susceptibility loci. Nat Genet 43(1):17-9. [PubMed: 21151127]  [MGI Ref ID J:169033]

Merino D; Giam M; Hughes PD; Siggs OM; Heger K; O'Reilly LA; Adams JM; Strasser A; Lee EF; Fairlie WD; Bouillet P. 2009. The role of BH3-only protein Bim extends beyond inhibiting Bcl-2-like prosurvival proteins. J Cell Biol 186(3):355-62. [PubMed: 19651893]  [MGI Ref ID J:153846]

Merino D; Khaw SL; Glaser SP; Anderson DJ; Belmont LD; Wong C; Yue P; Robati M; Phipson B; Fairlie WD; Lee EF; Campbell KJ; Vandenberg CJ; Cory S; Roberts AW; Ludlam MJ; Huang DC; Bouillet P. 2012. Bcl-2, Bcl-x(L), and Bcl-w are not equivalent targets of ABT-737 and navitoclax (ABT-263) in lymphoid and leukemic cells. Blood 119(24):5807-16. [PubMed: 22538851]  [MGI Ref ID J:188645]

Murphy BM; Engel T; Paucard A; Hatazaki S; Mouri G; Tanaka K; Tuffy LP; Jimenez-Mateos EM; Woods I; Dunleavy M; Bonner HP; Meller R; Simon RP; Strasser A; Prehn JH; Henshall DC. 2010. Contrasting patterns of Bim induction and neuroprotection in Bim-deficient mice between hippocampus and neocortex after status epilepticus. Cell Death Differ 17(3):459-68. [PubMed: 19779495]  [MGI Ref ID J:169433]

Nagase Y; Iwasawa M; Akiyama T; Kadono Y; Nakamura M; Oshima Y; Yasui T; Matsumoto T; Hirose J; Nakamura H; Miyamoto T; Bouillet P; Nakamura K; Tanaka S. 2009. Anti-apoptotic molecule Bcl-2 regulates the differentiation, activation, and survival of both osteoblasts and osteoclasts. J Biol Chem 284(52):36659-69. [PubMed: 19846553]  [MGI Ref ID J:158200]

Naik E; Michalak EM; Villunger A; Adams JM; Strasser A. 2007. Ultraviolet radiation triggers apoptosis of fibroblasts and skin keratinocytes mainly via the BH3-only protein Noxa. J Cell Biol 176(4):415-24. [PubMed: 17283183]  [MGI Ref ID J:119726]

Nordigarden A; Kraft M; Eliasson P; Labi V; Lam EW; Villunger A; Jonsson JI. 2009. BH3-only protein Bim more critical than Puma in tyrosine kinase inhibitor-induced apoptosis of human leukemic cells and transduced hematopoietic progenitors carrying oncogenic FLT3. Blood 113(10):2302-11. [PubMed: 19064725]  [MGI Ref ID J:146067]

Ohgushi M; Kuroki S; Fukamachi H; O'Reilly LA; Kuida K; Strasser A; Yonehara S. 2005. Transforming growth factor beta-dependent sequential activation of Smad, Bim, and caspase-9 mediates physiological apoptosis in gastric epithelial cells. Mol Cell Biol 25(22):10017-28. [PubMed: 16260615]  [MGI Ref ID J:102384]

Oliver PM; Vass T; Kappler J; Marrack P. 2006. Loss of the proapoptotic protein, Bim, breaks B cell anergy. J Exp Med 203(3):731-41. [PubMed: 16520387]  [MGI Ref ID J:123717]

Oliver PM; Wang M; Zhu Y; White J; Kappler J; Marrack P. 2004. Loss of Bim allows precursor B cell survival but not precursor B cell differentiation in the absence of interleukin 7. J Exp Med 200(9):1179-87. [PubMed: 15520248]  [MGI Ref ID J:94909]

Ou X; Xu S; Lam KP. 2012. Deficiency in TNFRSF13B (TACI) expands T-follicular helper and germinal center B cells via increased ICOS-ligand expression but impairs plasma cell survival. Proc Natl Acad Sci U S A 109(38):15401-6. [PubMed: 22949644]  [MGI Ref ID J:190155]

Pandiyan P; Zheng L; Ishihara S; Reed J; Lenardo MJ. 2007. CD4(+)CD25(+)Foxp3(+) regulatory T cells induce cytokine deprivation-mediated apoptosis of effector CD4(+) T cells. Nat Immunol 8(12):1353-62. [PubMed: 17982458]  [MGI Ref ID J:127761]

Pellegrini M; Belz G; Bouillet P; Strasser A. 2003. Shutdown of an acute T cell immune response to viral infection is mediated by the proapoptotic Bcl-2 homology 3-only protein Bim. Proc Natl Acad Sci U S A 100(24):14175-80. [PubMed: 14623954]  [MGI Ref ID J:86700]

Pellegrini M; Bouillet P; Robati M; Belz GT; Davey GM; Strasser A. 2004. Loss of Bim increases T cell production and function in interleukin 7 receptor-deficient mice. J Exp Med 200(9):1189-95. [PubMed: 15504823]  [MGI Ref ID J:94907]

Perier C; Bove J; Wu DC; Dehay B; Choi DK; Jackson-Lewis V; Rathke-Hartlieb S; Bouillet P; Strasser A; Schulz JB; Przedborski S; Vila M. 2007. Two molecular pathways initiate mitochondria-dependent dopaminergic neurodegeneration in experimental Parkinson's disease. Proc Natl Acad Sci U S A 104(19):8161-6. [PubMed: 17483459]  [MGI Ref ID J:121592]

Ramos SJ; Hernandez JB; Gatzka M; Walsh CM. 2008. Enhanced T cell apoptosis within Drak2-deficient mice promotes resistance to autoimmunity. J Immunol 181(11):7606-16. [PubMed: 19017949]  [MGI Ref ID J:142201]

Raynor J; Sholl A; Plas DR; Bouillet P; Chougnet CA; Hildeman DA. 2013. IL-15 Fosters Age-Driven Regulatory T Cell Accrual in the Face of Declining IL-2 Levels. Front Immunol 4:161. [PubMed: 23805138]  [MGI Ref ID J:208318]

Reckling S; Divanovic S; Karp CL; Wojciechowski S; Belkaid Y; Hildeman D. 2008. Proapoptotic Bcl-2 family member Bim promotes persistent infection and limits protective immunity. Infect Immun 76(3):1179-85. [PubMed: 18086806]  [MGI Ref ID J:131639]

Redmond WL; Wei CH; Kreuwel HT; Sherman LA. 2008. The apoptotic pathway contributing to the deletion of naive CD8 T cells during the induction of peripheral tolerance to a cross-presented self-antigen. J Immunol 180(8):5275-82. [PubMed: 18390708]  [MGI Ref ID J:134242]

Richter-Larrea JA; Robles EF; Fresquet V; Beltran E; Rullan AJ; Agirre X; Calasanz MJ; Panizo C; Richter JA; Hernandez JM; Roman-Gomez J; Prosper F; Martinez-Climent JA. 2010. Reversion of epigenetically mediated BIM silencing overcomes chemoresistance in Burkitt lymphoma. Blood 116(14):2531-42. [PubMed: 20570860]  [MGI Ref ID J:165889]

Ruppert SM; Li W; Zhang G; Carlson AL; Limaye A; Durum SK; Khaled AR. 2012. The major isoforms of Bim contribute to distinct biological activities that govern the processes of autophagy and apoptosis in interleukin-7 dependent lymphocytes. Biochim Biophys Acta 1823(10):1877-93. [PubMed: 22728771]  [MGI Ref ID J:188054]

Sandoval H; Thiagarajan P; Dasgupta SK; Schumacher A; Prchal JT; Chen M; Wang J. 2008. Essential role for Nix in autophagic maturation of erythroid cells. Nature 454(7201):232-5. [PubMed: 18454133]  [MGI Ref ID J:137609]

Santidrian AF; Gonzalez-Girones DM; Iglesias-Serret D; Coll-Mulet L; Cosialls AM; de Frias M; Campas C; Gonzalez-Barca E; Alonso E; Labi V; Viollet B; Benito A; Pons G; Villunger A; Gil J. 2010. AICAR induces apoptosis independently of AMPK and p53 through up-regulation of the BH3-only proteins BIM and NOXA in chronic lymphocytic leukemia cells. Blood 116(16):3023-32. [PubMed: 20664053]  [MGI Ref ID J:165862]

Schweers RL; Zhang J; Randall MS; Loyd MR; Li W; Dorsey FC; Kundu M; Opferman JT; Cleveland JL; Miller JL; Ney PA. 2007. NIX is required for programmed mitochondrial clearance during reticulocyte maturation. Proc Natl Acad Sci U S A 104(49):19500-5. [PubMed: 18048346]  [MGI Ref ID J:141545]

Sheibani N; Morrison ME; Gurel Z; Park S; Sorenson CM. 2012. BIM deficiency differentially impacts the function of kidney endothelial and epithelial cells through modulation of their local microenvironment. Am J Physiol Renal Physiol 302(7):F809-19. [PubMed: 22169007]  [MGI Ref ID J:182456]

Siggs OM; Arnold CN; Huber C; Pirie E; Xia Y; Lin P; Nemazee D; Beutler B. 2011. The P4-type ATPase ATP11C is essential for B lymphopoiesis in adult bone marrow. Nat Immunol 12(5):434-40. [PubMed: 21423172]  [MGI Ref ID J:171925]

Siggs OM; Li X; Xia Y; Beutler B. 2012. ZBTB1 is a determinant of lymphoid development J Exp Med :. [PubMed: 22201126]  [MGI Ref ID J:178820]

Siggs OM; Schnabl B; Webb B; Beutler B. 2011. X-linked cholestasis in mouse due to mutations of the P4-ATPase ATP11C. Proc Natl Acad Sci U S A 108(19):7890-5. [PubMed: 21518881]  [MGI Ref ID J:172799]

Silva DG; Daley SR; Hogan J; Lee SK; Teh CE; Hu DY; Lam KP; Goodnow CC; Vinuesa CG. 2011. Anti-islet autoantibodies trigger autoimmune diabetes in the presence of an increased frequency of islet-reactive CD4 T cells. Diabetes 60(8):2102-11. [PubMed: 21788582]  [MGI Ref ID J:186792]

Singh N; Yamamoto M; Takami M; Seki Y; Takezaki M; Mellor AL; Iwashima M. 2010. CD4(+)CD25(+) regulatory T cells resist a novel form of CD28- and Fas-dependent p53-induced T cell apoptosis. J Immunol 184(1):94-104. [PubMed: 19949106]  [MGI Ref ID J:159002]

Staton TL; Lazarevic V; Jones DC; Lanser AJ; Takagi T; Ishii S; Glimcher LH. 2011. Dampening of death pathways by schnurri-2 is essential for T-cell development. Nature 472(7341):105-9. [PubMed: 21475200]  [MGI Ref ID J:170984]

Steckley D; Karajgikar M; Dale LB; Fuerth B; Swan P; Drummond-Main C; Poulter MO; Ferguson SS; Strasser A; Cregan SP. 2007. Puma is a dominant regulator of oxidative stress induced Bax activation and neuronal apoptosis. J Neurosci 27(47):12989-99. [PubMed: 18032672]  [MGI Ref ID J:127640]

Swiecki M; Wang Y; Vermi W; Gilfillan S; Schreiber RD; Colonna M. 2011. Type I interferon negatively controls plasmacytoid dendritic cell numbers in vivo. J Exp Med 208(12):2367-74. [PubMed: 22084408]  [MGI Ref ID J:178613]

Szymczak-Workman AL; Delgoffe GM; Green DR; Vignali DA. 2011. Cutting edge: Regulatory T cells do not mediate suppression via programmed cell death pathways. J Immunol 187(9):4416-20. [PubMed: 21949016]  [MGI Ref ID J:179446]

Tischner D; Wiegers GJ; Fiegl H; Drach M; Villunger A. 2012. Mutual antagonism of TGF-beta and Interleukin-2 in cell survival and lineage commitment of induced regulatory T cells. Cell Death Differ 19(8):1277-87. [PubMed: 22322859]  [MGI Ref ID J:204633]

Tsuchiya T; Bonner HP; Engel T; Woods I; Matsushima S; Ward MW; Taki W; Henshall DC; Concannon CG; Prehn JH. 2011. Bcl-2 homology domain 3-only proteins Puma and Bim mediate the vulnerability of CA1 hippocampal neurons to proteasome inhibition in vivo. Eur J Neurosci 33(3):401-8. [PubMed: 21198986]  [MGI Ref ID J:174462]

Tsukamoto H; Huston GE; Dibble J; Duso DK; Swain SL. 2010. Bim dictates naive CD4 T cell lifespan and the development of age-associated functional defects. J Immunol 185(8):4535-44. [PubMed: 20844198]  [MGI Ref ID J:164881]

Uldrich AP; Berzins SP; Malin MA; Bouillet P; Strasser A; Smyth MJ; Boyd RL; Godfrey DI. 2006. Antigen challenge inhibits thymic emigration. J Immunol 176(8):4553-61. [PubMed: 16585545]  [MGI Ref ID J:131166]

Uldrich AP; Crowe NY; Kyparissoudis K; Pellicci DG; Zhan Y; Lew AM; Bouillet P; Strasser A; Smyth MJ; Godfrey DI. 2005. NKT cell stimulation with glycolipid antigen in vivo: costimulation-dependent expansion, Bim-dependent contraction, and hyporesponsiveness to further antigenic challenge. J Immunol 175(5):3092-101. [PubMed: 16116198]  [MGI Ref ID J:113331]

Van Dyken SJ; Green RS; Marth JD. 2007. Structural and mechanistic features of protein O glycosylation linked to CD8+ T-cell apoptosis. Mol Cell Biol 27(3):1096-111. [PubMed: 17101770]  [MGI Ref ID J:118162]

Villunger A; Marsden VS; Zhan Y; Erlacher M; Lew AM; Bouillet P; Berzins S; Godfrey DI; Heath WR; Strasser A. 2004. Negative selection of semimature CD4(+)8(-)HSA+ thymocytes requires the BH3-only protein Bim but is independent of death receptor signaling. Proc Natl Acad Sci U S A 101(18):7052-7. [PubMed: 15118096]  [MGI Ref ID J:89947]

Wang JQ; Jeelall YS; Beutler B; Horikawa K; Goodnow CC. 2014. Consequences of the recurrent MYD88(L265P) somatic mutation for B cell tolerance. J Exp Med 211(3):413-26. [PubMed: 24534189]  [MGI Ref ID J:210476]

Wang S; Park S; Fei P; Sorenson CM. 2011. Bim is responsible for the inherent sensitivity of the developing retinal vasculature to hyperoxia. Dev Biol 349(2):296-309. [PubMed: 21047504]  [MGI Ref ID J:168031]

Weant AE; Michalek RD; Crump KE; Liu C; Konopitski AP; Grayson JM. 2011. Defects in apoptosis increase memory CD8(+) T cells following infection of Bim(-/-)Fas(lpr/lpr) mice. Cell Immunol 271(2):256-66. [PubMed: 21839428]  [MGI Ref ID J:176721]

Weant AE; Michalek RD; Khan IU; Holbrook BC; Willingham MC; Grayson JM. 2008. Apoptosis regulators Bim and Fas function concurrently to control autoimmunity and CD8+ T cell contraction. Immunity 28(2):218-30. [PubMed: 18275832]  [MGI Ref ID J:132216]

Williams EL; Tutt AL; French RR; Chan HT; Lau B; Penfold CA; Mockridge CI; Roghanian A; Cox KL; Verbeek JS; Glennie MJ; Cragg MS. 2012. Development and characterisation of monoclonal antibodies specific for the murine inhibitory FcgammaRIIB (CD32B). Eur J Immunol 42(8):2109-20. [PubMed: 22760702]  [MGI Ref ID J:187928]

Wojciechowski S; Jordan MB; Zhu Y; White J; Zajac AJ; Hildeman DA. 2006. Bim mediates apoptosis of CD127(lo) effector T cells and limits T cell memory. Eur J Immunol 36(7):1694-706. [PubMed: 16761315]  [MGI Ref ID J:115833]

Wojciechowski S; Tripathi P; Bourdeau T; Acero L; Grimes HL; Katz JD; Finkelman FD; Hildeman DA. 2007. Bim/Bcl-2 balance is critical for maintaining naive and memory T cell homeostasis. J Exp Med 204(7):1665-75. [PubMed: 17591857]  [MGI Ref ID J:125868]

Xiang Z; Cutler AJ; Brownlie RJ; Fairfax K; Lawlor KE; Severinson E; Walker EU; Manz RA; Tarlinton DM; Smith KG. 2007. FcgammaRIIb controls bone marrow plasma cell persistence and apoptosis. Nat Immunol 8(4):419-29. [PubMed: 17322888]  [MGI Ref ID J:120734]

Xiao C; Srinivasan L; Calado DP; Patterson HC; Zhang B; Wang J; Henderson JM; Kutok JL; Rajewsky K. 2008. Lymphoproliferative disease and autoimmunity in mice with increased miR-17-92 expression in lymphocytes. Nat Immunol 9(4):405-14. [PubMed: 18327259]  [MGI Ref ID J:133215]

Xing Y; Jameson SC; Hogquist KA. 2013. Thymoproteasome subunit-beta5T generates peptide-MHC complexes specialized for positive selection. Proc Natl Acad Sci U S A 110(17):6979-84. [PubMed: 23569244]  [MGI Ref ID J:196156]

Yano T; Ito K; Fukamachi H; Chi XZ; Wee HJ; Inoue K; Ida H; Bouillet P; Strasser A; Bae SC; Ito Y. 2006. The RUNX3 tumor suppressor upregulates Bim in gastric epithelial cells undergoing transforming growth factor beta-induced apoptosis. Mol Cell Biol 26(12):4474-88. [PubMed: 16738314]  [MGI Ref ID J:109611]

de Kouchkovsky D; Esensten JH; Rosenthal WL; Morar MM; Bluestone JA; Jeker LT. 2013. microRNA-17-92 regulates IL-10 production by regulatory T cells and control of experimental autoimmune encephalomyelitis. J Immunol 191(4):1594-605. [PubMed: 23858035]  [MGI Ref ID J:205831]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & HusbandryThe resulting chimeric animals were crossed to a C57BL/6 Cre-deleter strain to remove the neo cassette, and then backcrossed to C57BL/6 for 13 generations. Donating Investigator reports that homozygous females, although fertile, tend to be poor mothers; uses the following breeding scheme: heterozygous females X homozygous males . 5-7 pups per litter.
Mating SystemHeterozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHeterozygous for Bcl2l11tm1.1Ast  
$239.00Female or MaleHomozygous for Bcl2l11tm1.1Ast  
Price per Pair (US dollars $)Pair Genotype
$478.00Heterozygous for Bcl2l11tm1.1Ast x Homozygous for Bcl2l11tm1.1Ast  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHeterozygous for Bcl2l11tm1.1Ast  
$310.70Female or MaleHomozygous for Bcl2l11tm1.1Ast  
Price per Pair (US dollars $)Pair Genotype
$621.40Heterozygous for Bcl2l11tm1.1Ast x Homozygous for Bcl2l11tm1.1Ast  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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