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Genes & Alleles

Npy;   Npytm1Rpa;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Richard Palmiter,   University of Washington Generation N?+F?+F10 (31-DEC-07)

Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by Northern or Western blot analysis of brain or adrenal gland tissue. Beta-galactosidase activity assays and in situ hybridization demonstrate similar expression patterns for the lacZ gene and the endogenous wildtype gene. Spontaneous seizures are exhibited by some mice at age 6 to 8 weeks. Homozygous mice are susceptible to seizures induced by GABA antagonist treatment. Mutant mice have an increased sensitivity to leptin treatment which results in a greater initial reduction of food intake and weight loss when compared to wildtype mice. This mutant mouse strain may be useful in studies related to the role of neuropeptide Y in obesity.

Strain Development
A targeting vector containing neomycin resistance, thymidine kinase and lacZ reporter genes was used to disrupt exon 2 of the targeted gene. The construct was electroporated into 129S6/SvEvBrd-Hprt⁺ derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.

Mammalian Phenotype Terms assigned by genotype Npytm1Rpa/Npytm1Rpa         involves: 129S7/SvEvBrd behavior/neurological phenotype sporadic seizures (J:33261) mice exhibited occasional mild seizures at 6-8 weeks of age, with decreased frequency until 9 weeks and were not seen thereafter; seizures consisted of jerking of the head and body, tail extension and vocalization susceptibility to pharmacologically induced seizures (J:33261) pentylenetrazole (PTZ) treatment at 50 or 60 mg/kg induced convulsions in 80% of mutant mice compared to 33% of controls convulsions in the mutant mice were also scored as more severe homeostasis/metabolism phenotype *normal* homeostasis/metabolism phenotype (J:33261) no defects were observed in food intake, body weight and adiposity normal glucose homeostasis was also noted responses to food deprivation was similar to controls susceptibility to pharmacologically induced seizures (J:33261) pentylenetrazole (PTZ) treatment at 50 or 60 mg/kg induced convulsions in 80% of mutant mice compared to 33% of controls convulsions in the mutant mice were also scored as more severe nervous system phenotype sporadic seizures (J:33261) mice exhibited occasional mild seizures at 6-8 weeks of age, with decreased frequency until 9 weeks and were not seen thereafter; seizures consisted of jerking of the head and body, tail extension and vocalization susceptibility to pharmacologically induced seizures (J:33261) pentylenetrazole (PTZ) treatment at 50 or 60 mg/kg induced convulsions in 80% of mutant mice compared to 33% of controls convulsions in the mutant mice were also scored as more severe

Gene & Allele Details

Allele Symbol		Npytm1Rpa
Allele Name		targeted mutation 1, Richard D Palmiter
Common Name(s)		Npy-;
Mutation Made By		Richard Palmiter,   University of Washington
Strain of Origin		129S7/SvEvBrd-Hprt1<+>
ES Cell Line Name		AB1
ES Cell Line Strain		129S7/SvEvBrd-Hprt1<+>
Site of Expression		lacZ replaces/mimics endogenous gene expression
Gene Symbol and Name		Npy, neuropeptide Y
Chromosome		6
Gene Common Name(s)		0710005A05Rik; NPY02; PYY4; RATNPY; RATNPY02; RIKEN cDNA 0710005A05 gene;
Molecular Note		Exon 2, which encodes the signal peptide and 35 amino acids, was replaced by a lacZ-neo fusion gene. The expression pattern of the beta-galactosidase was shown to resemble that of the endogenous gene via comparisons of X-gal staining and previous in situhybridization studies. RT-PCR analysis of brain tissue showed an absence of transcript in homozygous mutant mice. [J:33261]

Control Information

Allele	Control
Npytm1Rpa	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls

Genotyping Protocols

Npy^tm1Rpa

Colony Maintenance

Breeding & Husbandry	The resulting chimeric animals were crossed to 129S1/SvImJ mice. This strain is maintained as a homozygote.
Diet Information	LabDiet® 5K52/5K67

Related Strains

lacZ Expression Strains

002484	129-Alpltm1Sor/J
002292	129-Gt(ROSA)26Sor/J
006050	129-Sirt6tm1Fwa/J
003451	129-Smad3tm1Par/J
003310	129S-Gt(ROSA)26Sortm1Sor/J
003383	129S-Nogtm1Amc/J
005091	129S-Pnpla6tm1Blw/J
007199	129S-Sgpl1Gt(ROSA)78Sor/J
003082	129S1/SvImJ-Bcl2tm1Mpin/J
004478	B6.129-Foxd1tm1Lai/J
006939	B6.129-Fut1tm1Sdo/J
005768	B6.129-Htr5atm1Dgen/J
002938	B6.129-Kdrtm1Jrt/J
004158	B6.129-Maftm1Gsb/J
006497	B6.129-Skiltm2Spw/J
005772	B6.129P2-Acvrl1tm1Dgen/J
006431	B6.129P2-Adam21tm1Dgen/J
005770	B6.129P2-Adamts4tm1Dgen/J
005771	B6.129P2-Adamts5tm1Dgen/J
005773	B6.129P2-Adcy3tm1Dgen/J
005774	B6.129P2-Adcy7tm1Dgen/J
005775	B6.129P2-Adipor2tm1Dgen/J
005776	B6.129P2-Avpr1atm1Dgen/J
005777	B6.129P2-Axltm1Dgen/J
005783	B6.129P2-Cacna1ctm1Dgen/J
005780	B6.129P2-Cacna2d3tm1Dgen/J
005781	B6.129P2-Cacng3tm1Dgen/J
005782	B6.129P2-Cacng4tm1Dgen/J
005784	B6.129P2-Capn5tm1Dgen/J
005785	B6.129P2-Capn7tm1Dgen/J
005792	B6.129P2-Ccr1l1tm1Dgen/J
005793	B6.129P2-Ccr6tm1Dgen/J
005794	B6.129P2-Ccr7tm1Dgen/J
005779	B6.129P2-Celsr2tm1Dgen/J
005797	B6.129P2-Chrna2tm1Dgen/J
005787	B6.129P2-Ctsctm1Dgen/J
005796	B6.129P2-Cxcr3tm1Dgen/J
005798	B6.129P2-Drd5tm1Dgen/J
005800	B6.129P2-Efemp2tm1Dgen/J
005801	B6.129P2-Esrratm1Dgen/J
005802	B6.129P2-Faim2tm1Dgen/J
006262	B6.129P2-Fut2tm1Sdo/J
005803	B6.129P2-Fzd1tm1Dgen/J
005804	B6.129P2-Fzd8tm1Dgen/J
005811	B6.129P2-Gabra3tm1Dgen/J
005812	B6.129P2-Gabra4tm1Dgen/J
005810	B6.129P2-Gabrptm1Dgen/J
005809	B6.129P2-Galr1tm1Dgen/J
005816	B6.129P2-Glra3tm1Dgen/J
005805	B6.129P2-Gpr151tm1Dgen/J
005806	B6.129P2-Gpr37tm1Dgen/J
005807	B6.129P2-Gpr6tm1Dgen/J
005813	B6.129P2-Grik5tm1Dgen/J
005808	B6.129P2-Grk5tm1Dgen/J
005814	B6.129P2-Grm1tm1Dgen/J
005815	B6.129P2-Grm3tm1Dgen/J
005817	B6.129P2-Gsk3btm1Dgen/J
005818	B6.129P2-Hcrtr1tm1Dgen/J
005767	B6.129P2-Htr4tm1Dgen/J
005769	B6.129P2-Htr7tm1Dgen/J
005830	B6.129P2-Kcnq2tm1Dgen/J
005821	B6.129P2-Lats2tm1Dgen/J
005822	B6.129P2-Lmbr1tm1Dgen/J
005850	B6.129P2-Mapkapk2tm1Dgen/J
005824	B6.129P2-Mmp17tm1Dgen/J
005825	B6.129P2-Mtmr1tm1Dgen/J
005778	B6.129P2-Naip1tm1Dgen/J
005826	B6.129P2-Ntsr1tm1Dgen/J
005829	B6.129P2-Pkd2l2tm1Dgen/J
005828	B6.129P2-Ppardtm1Dgen/J
005831	B6.129P2-Ppm1ftm1Dgen/J
005827	B6.129P2-Ptch2tm1Dgen/J
005832	B6.129P2-Ptprotm1Dgen/J
005799	B6.129P2-S1pr4tm1Dgen/J
005837	B6.129P2-Scn11atm1Dgen/J
005836	B6.129P2-Scn9atm1Dgen/J
005834	B6.129P2-Sema5atm1Dgen/J
005835	B6.129P2-Sema6ctm1Dgen/J
006432	B6.129P2-Slc18a1tm1Dgen/J
005839	B6.129P2-Slc22a12tm1Dgen/J
005838	B6.129P2-Slc22a6tm1Dgen/J
005840	B6.129P2-Slc40a1tm1Dgen/J
005841	B6.129P2-Slc6a9tm1Dgen/J
005842	B6.129P2-Slc7a8tm1Dgen/J
005843	B6.129P2-Slc9a6tm1Dgen/J
005844	B6.129P2-Sstr1tm1Dgen/J
005847	B6.129P2-Tgfbr1tm1Dgen/J
005845	B6.129P2-Thbs4tm1Dgen/J
005790	B6.129P2-Tpp1tm1Dgen/J
005848	B6.129P2-Trpm5tm1Dgen/J
005791	B6.129P2-Xcr1tm1Dgen/J
003474	B6.129S4-Gt(ROSA)26Sortm1Sor/J
005901	B6.129S4-Ppardtm2Rev/J
006142	B6.129S4-Ppargtm1Rev/J
003754	B6.129S4-Shroom3Gt(ROSA)53Sor/J
005119	B6.129S6-Npas2tm1Slm/J
002741	B6.129S7-Alpltm1Sor/J
005970	B6.129S7-Atoh1tm2Hzo/J
006039	B6.129S7-Efnb2tm1And/J
002192	B6.129S7-Gt(ROSA)26Sor/J
005981	B6.129S7-Rai1tm1Jrl/J
005039	B6.129X1-Adra1atm1Pcs/J
005085	B6.Cg-Cd44tm1Hbg/J
007745	B6.Cg-Mirn155tm1.1Rsky/J
005317	B6.Cg-Tg(BAT-lacZ)3Picc/J
006055	B6.Cg-Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
004178	B6.Cg-Tg(CAG-Bgeo/GFP)21Lbe/J
006477	B6.Cg-Tg(CAG-lacZ-WGA)330Bbm/J
003139	B6.Cg-Tg(DBHn-lacZ)8Rpk/J
006229	B6.Cg-Tg(DRE-lacZ)2Gswz/J
002982	B6.Cg-Tg(xstpx-lacZ)32And/J
003504	B6;129-Gt(ROSA)26Sortm1Sho/J
005064	B6;129-Slc30a3tm1Rpa/J
005788	B6;129P2-Cd97tm1Dgen/J
005833	B6;129P2-Rgs4tm1Dgen/J
002073	B6;129S-Gt(ROSA)26Sor/J
006470	B6;129S-Hopxtm1Eno/J
004153	B6;129S-Mtap7Gt(ROSABetageo)1Sor/J
006958	B6;129S-Nkd1tm1Kwha/J
006960	B6;129S-Nkd2tm1Kwha/J
007204	B6;129S4-2610005L07RikGt(ROSA)73Sor/J
003309	B6;129S4-Gt(ROSA)26Sortm1Sor/J
004365	B6;129S6-Srebf1tm1Mbr/J
002317	B6;129S7-Alpltm1Sor/J
003266	B6;129S7-Epas1tm1Rus/J
006044	B6;129S7-Ephb4tm1And/J
003471	B6;C3H-Tg(CNP-GEO)1Ldh/J
006465	B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J
006680	B6;CBA-Tg(Olfr16*,taulacZ)19Mom/MomJ
006671	B6;CBA-Tg(Olfr16*,taulacZ)5Mom/MomJ
006672	B6;CBA-Tg(Olfr16*,taulacZ)7Mom/MomJ
006673	B6;CBA-Tg(Olfr16,taulacZ)sn2Mom/MomJ
004141	B6;CBA-Tg(UAS-lacZ)65Rth/J
002369	B6;SJL-Tg(c177-lacZ)226Bri/J
002372	B6;SJL-Tg(c177-lacZ)227Bri/J
002621	B6;SJL-Tg(tetop-lacZ)2Mam/J
003299	B6;SWJ-Tg(TIMP3-lacZ)7Jeb/J
002865	B6CBA-Tg(Wnt1-lacZ)206Amc/J
002955	C.129S7-Gt(ROSA)26Sor/J
002754	C57BL/6-Tg(LacZpl)60Vij/J
002193	C57BL/6J-Tg(MTn-lacZ)204Bri/J
002981	DBA/2-Tg(xstpx-lacZ)36And/J
004127	FVB-Tg(Nes-rtTA)306Rvs/J
007225	FVB.129(B6)-Usp18tm1Dzh/J
008203	FVB.Cg-Smn1tm1Msd Tg(ACTA1-SMN)63Ahmb Tg(SMN2)89Ahmb/J
008209	FVB.Cg-Smn1tm1Msd Tg(ACTA1-SMN)69Ahmb Tg(SMN2)89Ahmb/J
006214	FVB.Cg-Smn1tm1Msd/J
005024	FVB.Cg-Tg(SMN2)89Ahmb Smn1tm1Msd/J
005026	FVB.Cg-Tg(SMN2)89Ahmb Tg(SMN1*A2G)2023Ahmb Smn1tm1Msd/J
005025	FVB.Cg-Tg(SMN2*delta7)4299Ahmb Tg(SMN2)89Ahmb Smn1tm1Msd/J
003140	FVB/N-Tg(PAI1-lacZ)1Jjb/J
002856	FVB/N-Tg(TIE2-lacZ)182Sato/J
005941	FVB/N-Tg(tetO-Aurkb,lacZ)41Kra/J
003315	FVB/N-Tg(tetORo1-lacZ)3Conk/J
003487	FVB/NJ-Tg(XGFAP-lacZ)3Mes/J
005878	NOD.Cg-Cd44tm1Hbg/J
003899	STOCK Cd44tm1Hbg/J
006241	STOCK Hhiptm1Amc/J
006578	STOCK Myoz2tm1Eno/J
005707	STOCK Rag1tm1Mom Tg(TIE2-lacZ)182Sato/J
008212	STOCK Smn1tm1Msd Tg(Prnp-SMN)92Ahmb Tg(SMN2)89Ahmb/J
006882	STOCK Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe/J
005438	STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
006850	STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876	STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
006613	STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J
003919	STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J
003920	STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
004623	STOCK Tg(Fos-lacZ)34Efu/J
006674	STOCK Tg(Olfr16,taulacZ)2030Mom/MomJ
005493	STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J
002395	STOCK Tg(Zfy1-lacZ)218Bri/J
003274	STOCK Tg(tetNZL)2Bjd/J
005728	STOCK Tg(tetO-Ipf1,lacZ)958.1Macd/J

View lacZ Expression Strains     (174 strains)

Strains carrying other alleles of Npy

006417

B6.FVB-Tg(Npy-hrGFP)1Lowl/J

View Strains carrying other alleles of Npy     (1 strain)

Additional Web Information

Fluorescent Proteins/lacZ Systems

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Obesity Without Diabetes (leptin hypersensitive)

Neurobiology Research
Channel and Transporter Defects
lacZ expression in neural tissue

Research Tools
Diabetes and Obesity Research (lacZ)
lacZ Expression

Npy^tm1Rpa related

Diabetes and Obesity Research
Obesity Without Diabetes (leptin hypersensitive)

Neurobiology Research
Epilepsy

Research Tools
Neurobiology Research (cell marker)

References

Selected Reference(s)

Erickson JC; Clegg KE; Palmiter RD. 1996. Sensitivity to leptin and susceptibility to seizures of mice lacking neuropeptide Y [see comments] Nature 381(6581):415-21. [PubMed: 8632796]  [J:33261]

Additional References

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Price and Supply Information

Strain Name:	129S-Npytm1Rpa/J
Stock Number:	004545

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

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For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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