Strain Name:

B6.129S1-Wnt3atm1Amc/J

Stock Number:

004581

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Andrew P McMahon,   University of Southern California

Description
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and, with the exception of a kinked tail, do not display any gross physical or behavioral abnormalities. Homozygous null mice have an embryonic lethal phenotype, failing to develop past embryonic days 10.5 to 12.5 dpc. The gene targeting strategy introduced a stop codon in exon 3 to produce a shortened inactive gene product. No full-length wildtype gene product (protein) is detected by Southern blot analysis of homozygous embryos. By 9.5 dpc homozygous mutant embryos exhibit loss or disruption of posterior structures caudal to forelimb bud, including truncated trunk, kinked neural tube, lost or disrupted somites and lack of tailbud formation.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 3 by introducing a stop codon. The construct was electroporated into 129S1/Sv-p+ Tyr+ KitlSl-J/+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts.

Related Strains

Strains carrying other alleles of Wnt3a
000553   B6EiC3Sn a/A-Egfrwa2 Wnt3avt/J
View Strains carrying other alleles of Wnt3a     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Wnt3atm1Amc/Wnt3atm1Amc

        involves: 129S1/Sv
  • mortality/aging
  • partial embryonic lethality during organogenesis
    • most homozygotes die around E10.5, but a few survive until just before birth   (MGI Ref ID J:69781)
  • skeleton phenotype
  • abnormal cervical vertebrae morphology   (MGI Ref ID J:69781)
    • abnormal cervical axis morphology
      • cervical vertebra 2 (C2) is fused to C1 in the dorsal region   (MGI Ref ID J:69781)
      • C2 has the anterior arcus atlantis that is normally seen on the ventral part of C1   (MGI Ref ID J:69781)
    • cervical vertebral transformation
      • homeotic transformation of cervical vertebra 2 (C2) to C1   (MGI Ref ID J:69781)
      • C2 has the anterior arcus atlantis that is normally seen on the ventral part of C1   (MGI Ref ID J:69781)
  • kyphosis
    • seen in surviving embryos at E18.5   (MGI Ref ID J:69781)
  • embryogenesis phenotype
  • abnormal somite development
    • only abnormal somite formation is seen posterior to the forelimb bud   (MGI Ref ID J:31335)
  • sirenomelia
    • truncation caudal to the forelimb level   (MGI Ref ID J:69781)
  • limbs/digits/tail phenotype
  • sirenomelia
    • truncation caudal to the forelimb level   (MGI Ref ID J:69781)

Wnt3atm1Amc/Wnt3atm1Amc

        involves: 129S1/Sv * C57BL/6J
  • mortality/aging
  • complete embryonic lethality during organogenesis
    • homozygous mutant embryos die between E10.5 and E12.5   (MGI Ref ID J:16716)
  • embryogenesis phenotype
  • abnormal gastrulation
    • at E9.5, severe axial truncations suggest impaired gastrulation   (MGI Ref ID J:16716)
    • abnormal mesoderm development
      • homozygous mutant embryos exhibit impaired dorsal mesoderm development   (MGI Ref ID J:16716)
  • abnormal rostral-caudal axis patterning
    • at E9.5, mutants show massive cell death in the caudal-most region of the axis; cell death is mainly restricted to the dorsal-most mesodermal cells   (MGI Ref ID J:16716)
    • at E12.5, caudal development terminates at the level of umbilicus, just anterior to where hindlimbs normally form   (MGI Ref ID J:16716)
    • caudal body truncation
      • at E9.5, all mutant embryos exhibit severe axial truncation; however, yolk sac mesoderm and allantois formation appears unaffected   (MGI Ref ID J:16716)
      • by E12.5, mutants with little or no caudal development posterior to the forelimb display massive axial truncation   (MGI Ref ID J:16716)
  • absent tail bud
    • at E9.5, homozygous mutant embryos lack a visible tail bud formation   (MGI Ref ID J:16716)
  • incomplete somite formation
    • at E9.5, formation of the first 7 to 9 somites up to the level of the forelimb bud is normal; however, no somites are detected from the forelimbs caudal   (MGI Ref ID J:16716)
  • kinked neural tube
    • at E9.5, mutant embryos have a severely kinked and folded neural tube   (MGI Ref ID J:16716)
    • at E9.75, dorsal fusion of the neural tube is often absent   (MGI Ref ID J:16716)
  • truncated notochord
    • at E9.5, mutant notochord is present at the level of forelimbs, but is missing or disrupted in more posterior regions just caudal to the forelimb buds   (MGI Ref ID J:16716)
  • nervous system phenotype
  • abnormal nervous system morphology
    • mutant embryos exhibit abnormal CNS morphology and ectopic expression of a dorsal CNS maker   (MGI Ref ID J:16716)
    • abnormal spinal cord morphology
      • at E9.5, the mutant spinal cord is severely dysmorphic (kinked)   (MGI Ref ID J:16716)
    • kinked neural tube
      • at E9.5, mutant embryos have a severely kinked and folded neural tube   (MGI Ref ID J:16716)
      • at E9.75, dorsal fusion of the neural tube is often absent   (MGI Ref ID J:16716)
  • growth/size/body phenotype
  • decreased body length
    • at E9.5, mutant embryos display a shortened trunk caudal to the forelimbs; anterior development proceeds normally   (MGI Ref ID J:16716)
  • limbs/digits/tail phenotype
  • absent tail bud
    • at E9.5, homozygous mutant embryos lack a visible tail bud formation   (MGI Ref ID J:16716)
  • absent tail
    • at E12.5, mutants occasionally display a small degenerate structure just caudal to the forelimbs, i.e. a single midline hindlimb in the normal position of the tail   (MGI Ref ID J:16716)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Wnt3atm1Amc related

Developmental Biology Research
Embryonic Lethality (Homozygous)

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Wnt3atm1Amc
Allele Name targeted mutation 1, Andrew P McMahon
Allele Type Targeted (Null/Knockout)
Common Name(s) Wnt-3a-; Wnt3a-; Wnt3aneo;
Mutation Made By Andrew McMahon,   University of Southern California
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line NameCJ7
ES Cell Line Strain129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Wnt3a, wingless-type MMTV integration site family, member 3A
Chromosome 11
Gene Common Name(s) Wnt-3a; vestigial tail; vt;
Molecular Note The insertion of neomycin selection cassette into exon 3 introduced a stop codon that results in a truncated peptide approximately one-third the normal size. Transcription of the targeted loci was detected in homozygous mutant embryos via in situ hybridization. [MGI Ref ID J:16716] [MGI Ref ID J:39344]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Takada S; Stark KL; Shea MJ; Vassileva G; McMahon JA; McMahon AP. 1994. Wnt-3a regulates somite and tailbud formation in the mouse embryo. Genes Dev 8(2):174-89. [PubMed: 8299937]  [MGI Ref ID J:16716]

Additional References

Greco TL; Takada S; Newhouse MM; McMahon JA; McMahon AP; Camper SA. 1996. Analysis of the vestigial tail mutation demonstrates that Wnt-3a gene dosage regulates mouse axial development. Genes Dev 10(3):313-24. [PubMed: 8595882]  [MGI Ref ID J:31335]

Wnt3atm1Amc related

Alvarez-Medina R; Cayuso J; Okubo T; Takada S; Marti E. 2008. Wnt canonical pathway restricts graded Shh/Gli patterning activity through the regulation of Gli3 expression. Development 135(2):237-47. [PubMed: 18057099]  [MGI Ref ID J:130428]

Bialecka M; Young T; Chuva de Sousa Lopes S; ten Berge D; Sanders A; Beck F; Deschamps J. 2012. Cdx2 contributes to the expansion of the early primordial germ cell population in the mouse. Dev Biol 371(2):227-34. [PubMed: 22960234]  [MGI Ref ID J:190539]

Biris KK; Dunty WC Jr; Yamaguchi TP. 2007. Mouse Ripply2 is downstream of Wnt3a and is dynamically expressed during somitogenesis. Dev Dyn 236(11):3167-72. [PubMed: 17937396]  [MGI Ref ID J:125766]

Camper SA; Greco TL; Newhouse MM; Takada S; McMahon AP. 1994. Wnt-3a is critical for caudal embryonic development Am J Hum Genet 55(Suppl):A238 (Abstr.).  [MGI Ref ID J:25690]

Caronia G; Wilcoxon J; Feldman P; Grove EA. 2010. Bone morphogenetic protein signaling in the developing telencephalon controls formation of the hippocampal dentate gyrus and modifies fear-related behavior. J Neurosci 30(18):6291-301. [PubMed: 20445055]  [MGI Ref ID J:160559]

Chalamalasetty RB; Dunty WC Jr; Biris KK; Ajima R; Iacovino M; Beisaw A; Feigenbaum L; Chapman DL; Yoon JK; Kyba M; Yamaguchi TP. 2011. The Wnt3a/beta-catenin target gene Mesogenin1 controls the segmentation clock by activating a Notch signalling program. Nat Commun 2:390. [PubMed: 21750544]  [MGI Ref ID J:205649]

Greco TL; Takada S; Newhouse MM; McMahon JA; McMahon AP; Camper SA. 1996. Analysis of the vestigial tail mutation demonstrates that Wnt-3a gene dosage regulates mouse axial development. Genes Dev 10(3):313-24. [PubMed: 8595882]  [MGI Ref ID J:31335]

Hadjantonakis AK; Pisano E; Papaioannou VE. 2008. Tbx6 regulates left/right patterning in mouse embryos through effects on nodal cilia and perinodal signaling. PLoS ONE 3(6):e2511. [PubMed: 18575602]  [MGI Ref ID J:137163]

Ikeya M; Lee SM; Johnson JE; McMahon AP; Takada S. 1997. Wnt signalling required for expansion of neural crest and CNS progenitors. Nature 389(6654):966-70. [PubMed: 9353119]  [MGI Ref ID J:44094]

Ikeya M; Takada S. 1998. Wnt signaling from the dorsal neural tube is required for the formation of the medial dermomyotome. Development 125(24):4969-76. [PubMed: 9811581]  [MGI Ref ID J:50279]

Ikeya M; Takada S. 2001. Wnt-3a is required for somite specification along the anteroposterior axis of the mouse embryo and for regulation of cdx-1 expression. Mech Dev 103(1-2):27-33. [PubMed: 11335109]  [MGI Ref ID J:69781]

Kamata T; Katsube K; Michikawa M; Yamada M; Takada S; Mizusawa H. 2004. R-spondin, a novel gene with thrombospondin type 1 domain, was expressed in the dorsal neural tube and affected in Wnts mutants Biochim Biophys Acta 1676(1):51-62. [PubMed: 14732490]  [MGI Ref ID J:77554]

Lee SM; Tole S; Grove E; McMahon AP. 2000. A local Wnt-3a signal is required for development of the mammalian hippocampus. Development 127(3):457-67. [PubMed: 10631167]  [MGI Ref ID J:59283]

Luis TC; Weerkamp F; Naber BA; Baert MR; de Haas EF; Nikolic T; Heuvelmans S; De Krijger RR; van Dongen JJ; Staal FJ. 2009. Wnt3a deficiency irreversibly impairs hematopoietic stem cell self-renewal and leads to defects in progenitor cell differentiation. Blood 113(3):546-54. [PubMed: 18832654]  [MGI Ref ID J:144426]

Nakaya MA; Biris K; Tsukiyama T; Jaime S; Rawls JA; Yamaguchi TP. 2005. Wnt3alinks left-right determination with segmentation and anteroposterior axis elongation. Development 132(24):5425-36. [PubMed: 16291790]  [MGI Ref ID J:104377]

Riccomagno MM; Takada S; Epstein DJ. 2005. Wnt-dependent regulation of inner ear morphogenesis is balanced by the opposing and supporting roles of Shh. Genes Dev 19(13):1612-23. [PubMed: 15961523]  [MGI Ref ID J:99408]

Sewell W; Sparrow DB; Smith AJ; Gonzalez DM; Rappaport EF; Dunwoodie SL; Kusumi K. 2009. Cyclical expression of the Notch/Wnt regulator Nrarp requires modulation by Dll3 in somitogenesis. Dev Biol 329(2):400-9. [PubMed: 19268448]  [MGI Ref ID J:148363]

Shimizu T; Kagawa T; Wada T; Muroyama Y; Takada S; Ikenaka K. 2005. Wnt signaling controls the timing of oligodendrocyte development in the spinal cord. Dev Biol 282(2):397-410. [PubMed: 15950605]  [MGI Ref ID J:104602]

Suriben R; Kivimae S; Fisher DA; Moon RT; Cheyette BN. 2009. Posterior malformations in Dact1 mutant mice arise through misregulated Vangl2 at the primitive streak. Nat Genet 41(9):977-85. [PubMed: 19701191]  [MGI Ref ID J:152701]

Yamaguchi TP; Takada S; Yoshikawa Y; Wu N; McMahon AP. 1999. T (Brachyury) is a direct target of Wnt3a during paraxial mesoderm specification. Genes Dev 13(24):3185-90. [PubMed: 10617567]  [MGI Ref ID J:59271]

Yoshikawa Y; Fujimori T; McMahon AP; Takada S. 1997. Evidence that absence of Wnt-3a signaling promotes neuralization instead of paraxial mesoderm development in the mouse. Dev Biol 183(2):234-42. [PubMed: 9126297]  [MGI Ref ID J:39344]

Yu H; Ye X; Guo N; Nathans J. 2012. Frizzled 2 and frizzled 7 function redundantly in convergent extension and closure of the ventricular septum and palate: evidence for a network of interacting genes. Development 139(23):4383-94. [PubMed: 23095888]  [MGI Ref ID J:189062]

van de Ven C; Bialecka M; Neijts R; Young T; Rowland JE; Stringer EJ; Van Rooijen C; Meijlink F; Novoa A; Freund JN; Mallo M; Beck F; Deschamps J. 2011. Concerted involvement of Cdx/Hox genes and Wnt signaling in morphogenesis of the caudal neural tube and cloacal derivatives from the posterior growth zone. Development 138(16):3451-62. [PubMed: 21752936]  [MGI Ref ID J:180902]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThe resulting chimeric male animals were crossed to C57BL/6J females. The strain is maintained as a heterozygote. The Donating Investigator reports that heterozygotes are poor breeders.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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