| |||||||||
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N8+N2F10 (04-DEC-07) Donating Investigator Ronald Evans, The Salk Inst for Biological Studies Description
These mice possess loxP sites on either side of exons 1 and 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.When bred to a strain expressing Cre recombinase in adipose tissue (see Stock No. 005069 for example), this mutant mouse strain may be useful in studies of insulin resistance.
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Strains carrying other alleles of Pparg
008079 129S-Ppargtm2Yba/J 006142 B6.129S4-Ppargtm1Rev/J 008227 B6.129S4-Ppargtm3Yba/J View Strains carrying other alleles of Pparg (3 strains)
Congenic Nomenclature
Cre-lox Systems
Genetic Quality Control Annual Report
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Ppargtm2Rev/Ppargtm2Rev Tg(Fabp4-cre)1Rev/?
involves: 129S4/SvJae * C57BL/6 (conditional)
- adipose tissue phenotype
- abnormal brown adipose tissue morphology (MGI Ref ID J:88210)
- cells are larger and variable in size
- cell numbers reduced more than 95%
- vacuole form sometimes like white adipose cells rather than typical brown fat cells
- decreased brown adipose tissue amount (MGI Ref ID J:88210)
- brown adipose tissue mass reduced more than 70% by 6 months of age
- abnormal interscapular fat pad morphology (MGI Ref ID J:88210)
- diminution of interscapular fat pads detectable by 4-6 weeks
- abnormal white adipose tissue morphology (MGI Ref ID J:88210)
- more than 50% of white adipocytes are highly hypertrophic
- abnormal white adipose tissue amount (MGI Ref ID J:88210)
- cell loss greater than 80%
- growth/size phenotype
- weight gain (MGI Ref ID J:88210)
- weight gain on a high fat diet is decreased
- homeostasis/metabolism phenotype
- increased circulating insulin level (MGI Ref ID J:88210)
- insulin levels are increased
- insulin resistance (MGI Ref ID J:88210)
- insulin became less efficient at suppressing glucose production
- liver/biliary system phenotype
- enlarged liver (MGI Ref ID J:88210)
- hepatomegally eventually develops as well
- hepatic steatosis (MGI Ref ID J:88210)
- livers were beginning to be steatotic at 6 months of age
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
Ppargtm2Rev relatedResearch Tools
Cre-lox System (loxP-flanked Sequences)
Diabetes and Obesity Research (loxP)
Metabolism Research
Research Tools
Cardiovascular Research (Cre-lox System)
Diabetes and Obesity Research (loxP)
| Allele Symbol | Ppargtm2Rev | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Ronald M Evans | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | PPARgammaf; Ppargflox; gf; | ||
| Mutation Made By | Yaacov Barak, The Jackson Laboratory | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Gene Symbol and Name | Pparg, peroxisome proliferator activated receptor gamma | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | NR1C3; PPAR-gamma; PPARG1; PPARG2; PPARgamma; PPARgamma2; Ppar-gamma2; | ||
| Molecular Note | A single loxP site was introduced upstream of exon 1 and a floxed neomycin cassette was inserted downstream of exon 2. The neomycin gene was excised from correctly targeted ES cells by transient transfection with a Cre expression plasmid. [MGI Ref ID J:88210] | ||
Genotyping Protocols
Ppargtm2Rev, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
He W; Barak Y; Hevener A; Olson P; Liao D; Le J; Nelson M; Ong E; Olefsky JM; Evans RM. 2003. Adipose-specific peroxisome proliferator-activated receptor gamma knockout causes insulin resistance in fat and liver but not in muscle. Proc Natl Acad Sci U S A 100(26):15712-7. [PubMed: 14660788] [MGI Ref ID J:88210]
Ppargtm2Rev relatedDavies BS; Waki H; Beigneux AP; Farber E; Weinstein MM; Wilpitz DC; Tai LJ; Evans RM; Fong LG; Tontonoz P; Young SG. 2008. The expression of GHIBP1, an endothelial cell binding site for lipoprotein lipase and chylomicrons, is induced by peroxisome proliferator-activated receptor-gamma. Mol Endocrinol 22(11):2496-504. [PubMed: 18787041] [MGI Ref ID J:141250]
Hansmann G; de Jesus Perez VA; Alastalo TP; Alvira CM; Guignabert C; Bekker JM; Schellong S; Urashima T; Wang L; Morrell NW; Rabinovitch M. 2008. An antiproliferative BMP-2/PPARgamma/apoE axis in human and murine SMCs and its role in pulmonary hypertension. J Clin Invest 118(5):1846-57. [PubMed: 18382765] [MGI Ref ID J:136168]
Luo W; Cao J; Li J; He W. 2008. Adipose tissue-specific PPARgamma deficiency increases resistance to oxidative stress. Exp Gerontol 43(3):154-63. [PubMed: 18083318] [MGI Ref ID J:132506]
Wan Y; Chong LW; Evans RM. 2007. PPAR-gamma regulates osteoclastogenesis in mice. Nat Med 13(12):1496-503. [PubMed: 18059282] [MGI Ref ID J:130475]
Wan Y; Saghatelian A; Chong LW; Zhang CL; Cravatt BF; Evans RM. 2007. Maternal PPAR{gamma} protects nursing neonates by suppressing the production of inflammatory milk. Genes Dev 21(15):1895-908. [PubMed: 17652179] [MGI Ref ID J:123403]
Yang L; Chan CC; Kwon OS; Liu S; McGhee J; Stimpson SA; Chen LZ; Harrington WW; Symonds WT; Rockey DC. 2006. Regulation of peroxisome proliferator-activated receptor-gamma in liver fibrosis. Am J Physiol Gastrointest Liver Physiol 291(5):G902-11. [PubMed: 16798724] [MGI Ref ID J:116893]
Animal Health Reports
Room Number AX12
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $104.80 Female or Male Homozygous for Ppargtm2Rev *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $209.60 Homozygous for Ppargtm2Rev x Homozygous for Ppargtm2Rev
| Supply Notes |
|
|---|
| Pricing for International shipping destinations |
|
Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $136.30 Female or Male Homozygous for Ppargtm2Rev *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $272.50 Homozygous for Ppargtm2Rev x Homozygous for Ppargtm2Rev
| Supply Notes |
|
|---|
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
|
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license.
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. THE LABORATORY EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.
In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.
MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.
Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.