Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   01-MAR-06 Species laboratory mouse Generation N8+N2F13 (30-DEC-08)   Donating Investigator Ronald Evans,   The Salk Inst for Biological Studies

Description
These mice possess loxP sites on either side of exons 1 and 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain expressing Cre recombinase in adipose tissue (see Stock No. 005069 for example), this mutant mouse strain may be useful in studies of insulin resistance.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Pparg

008079	129S-Ppargtm2Yba/J
006142	B6.129S4-Ppargtm1Rev/J
008227	B6.129S4-Ppargtm3Yba/J

View Strains carrying other alleles of Pparg     (3 strains)

Additional Web Information

Genetic Quality Control Annual Report
Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX^® Services jaxservices@jax.org for customized breeding options.

Pparg^tm2Rev/Pparg^tm2Rev Tg(Fabp4-cre)1Rev/?

        involves: 129S4/SvJae * C57BL/6   (conditional)

• adipose tissue phenotype
• abnormal brown adipose tissue morphology (MGI Ref ID J:88210)
  • vacuole form sometimes like white adipose cells rather than typical brown fat cells
• abnormal brown fat cell size (MGI Ref ID J:88210)
• increased brown fat cell size (MGI Ref ID J:88210)
  • cells are larger and variable in size
• decreased brown adipose tissue amount (MGI Ref ID J:88210)
  • brown adipose tissue mass reduced more than 70% by 6 months of age
• decreased brown fat cell number (MGI Ref ID J:88210)
  • cell numbers reduced more than 95%
• abnormal white adipose tissue amount (MGI Ref ID J:88210)
  • cell loss greater than 80%
• decreased interscapular fat pad weight (MGI Ref ID J:88210)
  • diminution of interscapular fat pads detectable by 4-6 weeks
• increased white fat cell size (MGI Ref ID J:88210)
  • more than 50% of white adipocytes are highly hypertrophic
• growth/size phenotype
• postnatal slow weight gain (MGI Ref ID J:88210)
  • weight gain on a high fat diet is decreased
• homeostasis/metabolism phenotype
• increased circulating insulin level (MGI Ref ID J:88210)
  • insulin levels are increased
• insulin resistance (MGI Ref ID J:88210)
  • insulin became less efficient at suppressing glucose production
• liver/biliary system phenotype
• enlarged liver (MGI Ref ID J:88210)
  • hepatomegaly eventually develops as well
• hepatic steatosis (MGI Ref ID J:88210)
  • livers were beginning to be steatotic at 6 months of age

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Diabetes and Obesity Research
      loxP
Metabolism Research

Pparg^tm2Rev related

Research Tools
Cardiovascular Research
      Cre-lox System
Diabetes and Obesity Research
      loxP

Genes & Alleles

Gene & Allele Information

Allele Symbol		Ppargtm2Rev
Allele Name		targeted mutation 2, Ronald M Evans
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		PPARgammaf; Ppargflox; gf;
Mutation Made By		Yaacov Barak,   The Jackson Laboratory
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Pparg, peroxisome proliferator activated receptor gamma
Chromosome		6
Gene Common Name(s)		CIMT1; GLM1; NR1C3; PPAR-gamma; PPARG1; PPARG2; PPARgamma; PPARgamma2; Ppar-gamma2;
Molecular Note		A single loxP site was introduced upstream of exon 1 and a floxed neomycin cassette was inserted downstream of exon 2. The neomycin gene was excised from correctly targeted ES cells by transient transfection with a Cre expression plasmid. [MGI Ref ID J:88210]

Genotyping

Genotyping Information

Genotyping Protocols

Pparg^tm2Rev, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

He W; Barak Y; Hevener A; Olson P; Liao D; Le J; Nelson M; Ong E; Olefsky JM; Evans RM. 2003. Adipose-specific peroxisome proliferator-activated receptor gamma knockout causes insulin resistance in fat and liver but not in muscle. Proc Natl Acad Sci U S A 100(26):15712-7. [PubMed: 14660788]  [MGI Ref ID J:88210]

Additional References

Pparg^tm2Rev related

Davies BS; Waki H; Beigneux AP; Farber E; Weinstein MM; Wilpitz DC; Tai LJ; Evans RM; Fong LG; Tontonoz P; Young SG. 2008. The expression of GHIBP1, an endothelial cell binding site for lipoprotein lipase and chylomicrons, is induced by peroxisome proliferator-activated receptor-gamma. Mol Endocrinol 22(11):2496-504. [PubMed: 18787041]  [MGI Ref ID J:141250]

Hansmann G; de Jesus Perez VA; Alastalo TP; Alvira CM; Guignabert C; Bekker JM; Schellong S; Urashima T; Wang L; Morrell NW; Rabinovitch M. 2008. An antiproliferative BMP-2/PPARgamma/apoE axis in human and murine SMCs and its role in pulmonary hypertension. J Clin Invest 118(5):1846-57. [PubMed: 18382765]  [MGI Ref ID J:136168]

Karnik P; Tekeste Z; McCormick TS; Gilliam AC; Price VH; Cooper KD; Mirmirani P. 2009. Hair follicle stem cell-specific PPARgamma deletion causes scarring alopecia. J Invest Dermatol 129(5):1243-57. [PubMed: 19052558]  [MGI Ref ID J:150977]

Klotz L; Burgdorf S; Dani I; Saijo K; Flossdorf J; Hucke S; Alferink J; Novak N; Beyer M; Mayer G; Langhans B; Klockgether T; Waisman A; Eberl G; Schultze J; Famulok M; Kolanus W; Glass C; Kurts C; Knolle PA. 2009. The nuclear receptor PPAR gamma selectively inhibits Th17 differentiation in a T cell-intrinsic fashion and suppresses CNS autoimmunity. J Exp Med 206(10):2079-89. [PubMed: 19737866]  [MGI Ref ID J:153358]

Klotz L; Dani I; Edenhofer F; Nolden L; Evert B; Paul B; Kolanus W; Klockgether T; Knolle P; Diehl L. 2007. Peroxisome proliferator-activated receptor gamma control of dendritic cell function contributes to development of CD4+ T cell anergy. J Immunol 178(4):2122-31. [PubMed: 17277116]  [MGI Ref ID J:143994]

Luo W; Cao J; Li J; He W. 2008. Adipose tissue-specific PPARgamma deficiency increases resistance to oxidative stress. Exp Gerontol 43(3):154-63. [PubMed: 18083318]  [MGI Ref ID J:132506]

Necela BM; Su W; Thompson EA. 2008. Toll-like receptor 4 mediates cross-talk between peroxisome proliferator-activated receptor gamma and nuclear factor-kappaB in macrophages. Immunology 125(3):344-58. [PubMed: 18422969]  [MGI Ref ID J:144451]

Wan Y; Chong LW; Evans RM. 2007. PPAR-gamma regulates osteoclastogenesis in mice. Nat Med 13(12):1496-503. [PubMed: 18059282]  [MGI Ref ID J:130475]

Wan Y; Saghatelian A; Chong LW; Zhang CL; Cravatt BF; Evans RM. 2007. Maternal PPAR{gamma} protects nursing neonates by suppressing the production of inflammatory milk. Genes Dev 21(15):1895-908. [PubMed: 17652179]  [MGI Ref ID J:123403]

Yang L; Chan CC; Kwon OS; Liu S; McGhee J; Stimpson SA; Chen LZ; Harrington WW; Symonds WT; Rockey DC. 2006. Regulation of peroxisome proliferator-activated receptor-gamma in liver fibrosis. Am J Physiol Gastrointest Liver Physiol 291(5):G902-11. [PubMed: 16798724]  [MGI Ref ID J:116893]

Zhao X; Strong R; Zhang J; Sun G; Tsien JZ; Cui Z; Grotta JC; Aronowski J. 2009. Neuronal PPARgamma deficiency increases susceptibility to brain damage after cerebral ischemia. J Neurosci 29(19):6186-95. [PubMed: 19439596]  [MGI Ref ID J:148757]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Mating System	Homozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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