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Description
Strain Information
Former Names B6;SJL-Tg(Vil-cre)997Gum (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation N5+18 (22-SEP-09)
Generation DefinitionsDonating Investigator Deborah Gumucio, University of Michigan Description
Mice hemizygous for this transgene express Cre recombinase under the direction of the mouse villin 1 promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in villi and crypt cells of the small and large intestines, closely patterning the endogenous gene expression. The Donating Investigator indicates that expression is generally continuous, but that a small amount of mosaicism is noted in the colon. Onset of transgene expression is at 12.5 dpc, which is delayed from the endogenous mouse Vil1 gene expression onset of 9.0 dpc. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of intestinal organogenesis.Development
A transgenic construct containing the coding domain of Cre recombinase, driven by a 12.4 kb fragment of the mouse villin 1 gene, and a metallothionein polyadenylation site sequence was injected into the pronuclei of fertilized C57BL/6J X SJL/J mouse eggs. Founder animals were bred to C57BL/6 mice and then backcrossed to the same for 5 generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (311 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Vil-cre)997Gum | ||
|---|---|---|---|
| Allele Name | transgene insertion 997, Deborah L Gumucio | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | 12.4KbVilCre; Tg(Vil-cre)997Gum; Vil-Cre; VilCre; Villin-cre; Villin.cre; VillinCre; | ||
| Mutation Made By | Blair Madison, University of Michigan | ||
| Strain of Origin | (C57BL/6J x SJL)F1 | ||
| Site of Expression | epithelial cells of the small and large intestines; onset of expression is at 12.5 d.p.c.; expression is generally continuous, but a small amount of mosaicism is noted in the colon | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Vil1, villin 1, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Vil-cre)997Gum, transgene insertion 997, Deborah L Gumucio | ||
| Chromosome | UN | ||
| Gene Common Name(s) | 12.4KbVilCre; Tg(Vil-cre)997Gum; Villin-cre; Villin.cre; VillinCre; | ||
| Driver Note | Vil | ||
| General Note | Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. | ||
| Molecular Note | A 12.4-kb promoter fragment from the villin 1 gene drives the expression of Cre recombinase. Recombination occurs in villi and crypt cells of the small and large intestines, closely patterning the endogenous gene expression. According to the corresponding author (J:78815), expression is generally continuous but a small amount of mosaicism is noted in the colon. Onset of transgene expression is at 12.5 dpc, which is delayed from the endogenous mouse Vil gene expression onset of 9.0 dpc. [MGI Ref ID J:78815] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Tg(Vil-cre)997Gum, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Madison BB; Dunbar L; Qiao XT; Braunstein K; Braunstein E; Gumucio DL. 2002. cis Elements of the Villin Gene Control Expression in Restricted Domains of the Vertical (Crypt) and Horizontal (Duodenum, Cecum) Axes of the Intestine. J Biol Chem 277(36):33275-83. [PubMed: 12065599] [MGI Ref ID J:78815]
Additional References
Tg(Vil-cre)997Gum relatedAllaire JM; Darsigny M; Marcoux SS; Roy SA; Schmouth JF; Umans L; Zwijsen A; Boudreau F; Perreault N. 2011. Loss of Smad5 leads to the disassembly of the apical junctional complex and increased susceptibility to experimental colitis. Am J Physiol Gastrointest Liver Physiol 300(4):G586-97. [PubMed: 21212325] [MGI Ref ID J:171163]
Auclair BA; Benoit YD; Rivard N; Mishina Y; Perreault N. 2007. Bone morphogenetic protein signaling is essential for terminal differentiation of the intestinal secretory cell lineage. Gastroenterology 133(3):887-96. [PubMed: 17678919] [MGI Ref ID J:128279]
Babeu JP; Darsigny M; Lussier CR; Boudreau F. 2009. Hepatocyte nuclear factor 4alpha contributes to an intestinal epithelial phenotype in vitro and plays a partial role in mouse intestinal epithelium differentiation. Am J Physiol Gastrointest Liver Physiol 297(1):G124-34. [PubMed: 19389805] [MGI Ref ID J:151206]
Basseres DS; Ebbs A; Levantini E; Baldwin AS. 2010. Requirement of the NF-kappaB subunit p65/RelA for K-Ras-induced lung tumorigenesis. Cancer Res 70(9):3537-46. [PubMed: 20406971] [MGI Ref ID J:159456]
Battle MA; Bondow BJ; Iverson MA; Adams SJ; Jandacek RJ; Tso P; Duncan SA. 2008. GATA4 is essential for jejunal function in mice. Gastroenterology 135(5):1676-1686.e1. [PubMed: 18812176] [MGI Ref ID J:145635]
Bennecke M; Kriegl L; Bajbouj M; Retzlaff K; Robine S; Jung A; Arkan MC; Kirchner T; Greten FR. 2010. Ink4a/Arf and oncogene-induced senescence prevent tumor progression during alternative colorectal tumorigenesis. Cancer Cell 18(2):135-46. [PubMed: 20708155] [MGI Ref ID J:163673]
Bollrath J; Phesse TJ; von Burstin VA; Putoczki T; Bennecke M; Bateman T; Nebelsiek T; Lundgren-May T; Canli O; Schwitalla S; Matthews V; Schmid RM; Kirchner T; Arkan MC; Ernst M; Greten FR. 2009. gp130-mediated Stat3 activation in enterocytes regulates cell survival and cell-cycle progression during colitis-associated tumorigenesis. Cancer Cell 15(2):91-102. [PubMed: 19185844] [MGI Ref ID J:144814]
Brunham LR; Kruit JK; Iqbal J; Fievet C; Timmins JM; Pape TD; Coburn BA; Bissada N; Staels B; Groen AK; Hussain MM; Parks JS; Kuipers F; Hayden MR. 2006. Intestinal ABCA1 directly contributes to HDL biogenesis in vivo. J Clin Invest 116(4):1052-62. [PubMed: 16543947] [MGI Ref ID J:107802]
Brunham LR; Kruit JK; Pape TD; Parks JS; Kuipers F; Hayden MR. 2006. Tissue-specific induction of intestinal ABCA1 expression with a liver X receptor agonist raises plasma HDL cholesterol levels. Circ Res 99(7):672-4. [PubMed: 16946132] [MGI Ref ID J:126507]
Byun DS; Ahmed N; Nasser S; Shin J; Al-Obaidi S; Goel S; Corner GA; Wilson AJ; Flanagan DJ; Williams DS; Augenlicht LH; Vincan E; Mariadason JM. 2011. Intestinal epithelial-specific PTEN inactivation results in tumor formation. Am J Physiol Gastrointest Liver Physiol 301(5):G856-64. [PubMed: 21836055] [MGI Ref ID J:178019]
Cadwell K; Liu JY; Brown SL; Miyoshi H; Loh J; Lennerz JK; Kishi C; Kc W; Carrero JA; Hunt S; Stone CD; Brunt EM; Xavier RJ; Sleckman BP; Li E; Mizushima N; Stappenbeck TS; Virgin HW 4th. 2008. A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells. Nature 456(7219):259-63. [PubMed: 18849966] [MGI Ref ID J:141419]
Cai J; Zhang N; Zheng Y; de Wilde RF; Maitra A; Pan D. 2010. The Hippo signaling pathway restricts the oncogenic potential of an intestinal regeneration program. Genes Dev 24(21):2383-8. [PubMed: 21041407] [MGI Ref ID J:165471]
Calcagno SR; Li S; Colon M; Kreinest PA; Thompson EA; Fields AP; Murray NR. 2008. Oncogenic K-ras promotes early carcinogenesis in the mouse proximal colon. Int J Cancer 122(11):2462-70. [PubMed: 18271008] [MGI Ref ID J:135568]
Chen C; Fang R; Davis C; Maravelias C; Sibley E. 2009. Pdx1 inactivation restricted to the intestinal epithelium in mice alters duodenal gene expression in enterocytes and enteroendocrine cells. Am J Physiol Gastrointest Liver Physiol 297(6):G1126-37. [PubMed: 19808654] [MGI Ref ID J:155907]
Cui Y; Niziolek PJ; Macdonald BT; Zylstra CR; Alenina N; Robinson DR; Zhong Z; Matthes S; Jacobsen CM; Conlon RA; Brommage R; Liu Q; Mseeh F; Powell DR; Yang QM; Zambrowicz B; Gerrits H; Gossen JA; He X; Bader M; Williams BO; Warman ML; Robling AG. 2011. Lrp5 functions in bone to regulate bone mass. Nat Med 17(6):684-91. [PubMed: 21602802] [MGI Ref ID J:172746]
Czeh M; Loddenkemper C; Shalapour S; Schon C; Robine S; Goldscheid E; Stein H; Schuler T; Willimsky G; Blankenstein T. 2010. The immune response to sporadic colorectal cancer in a novel mouse model. Oncogene 29(50):6591-602. [PubMed: 20818425] [MGI Ref ID J:167345]
Darsigny M; Babeu JP; Dupuis AA; Furth EE; Seidman EG; Levy E; Verdu EF; Gendron FP; Boudreau F. 2009. Loss of hepatocyte-nuclear-factor-4alpha affects colonic ion transport and causes chronic inflammation resembling inflammatory bowel disease in mice. PLoS One 4(10):e7609. [PubMed: 19898610] [MGI Ref ID J:158289]
Darsigny M; Babeu JP; Seidman EG; Gendron FP; Levy E; Carrier J; Perreault N; Boudreau F. 2010. Hepatocyte nuclear factor-4alpha promotes gut neoplasia in mice and protects against the production of reactive oxygen species. Cancer Res 70(22):9423-33. [PubMed: 21062980] [MGI Ref ID J:166855]
Davies PS; Powell AE; Swain JR; Wong MH. 2009. Inflammation and proliferation act together to mediate intestinal cell fusion. PLoS One 4(8):e6530. [PubMed: 19657387] [MGI Ref ID J:152479]
Eckmann L; Nebelsiek T; Fingerle AA; Dann SM; Mages J; Lang R; Robine S; Kagnoff MF; Schmid RM; Karin M; Arkan MC; Greten FR. 2008. Opposing functions of IKKbeta during acute and chronic intestinal inflammation. Proc Natl Acad Sci U S A 105(39):15058-63. [PubMed: 18815378] [MGI Ref ID J:143368]
Firestein R; Blander G; Michan S; Oberdoerffer P; Ogino S; Campbell J; Bhimavarapu A; Luikenhuis S; de Cabo R; Fuchs C; Hahn WC; Guarente LP; Sinclair DA. 2008. The SIRT1 deacetylase suppresses intestinal tumorigenesis and colon cancer growth. PLoS ONE 3(4):e2020. [PubMed: 18414679] [MGI Ref ID J:134301]
Fu J; Wei B; Wen T; Johansson ME; Liu X; Bradford E; Thomsson KA; McGee S; Mansour L; Tong M; McDaniel JM; Sferra TJ; Turner JR; Chen H; Hansson GC; Braun J; Xia L. 2011. Loss of intestinal core 1-derived O-glycans causes spontaneous colitis in mice. J Clin Invest 121(4):1657-66. [PubMed: 21383503] [MGI Ref ID J:172017]
Fujishima Y; Nishiumi S; Masuda A; Inoue J; Nguyen NM; Irino Y; Komatsu M; Tanaka K; Kutsumi H; Azuma T; Yoshida M. 2011. Autophagy in the intestinal epithelium reduces endotoxin-induced inflammatory responses by inhibiting NF-kappaB activation. Arch Biochem Biophys 506(2):223-35. [PubMed: 21156154] [MGI Ref ID J:169251]
Galy B; Ferring-Appel D; Kaden S; Grone HJ; Hentze MW. 2008. Iron regulatory proteins are essential for intestinal function and control key iron absorption molecules in the duodenum. Cell Metab 7(1):79-85. [PubMed: 18177727] [MGI Ref ID J:131013]
Gao N; Kaestner KH. 2010. Cdx2 regulates endo-lysosomal function and epithelial cell polarity. Genes Dev 24(12):1295-305. [PubMed: 20551175] [MGI Ref ID J:161201]
Ghaleb AM; McConnell BB; Kaestner KH; Yang VW. 2011. Altered intestinal epithelial homeostasis in mice with intestine-specific deletion of the Kruppel-like factor 4 gene. Dev Biol 349(2):310-20. [PubMed: 21070761] [MGI Ref ID J:168026]
Grivennikov S; Karin E; Terzic J; Mucida D; Yu GY; Vallabhapurapu S; Scheller J; Rose-John S; Cheroutre H; Eckmann L; Karin M. 2009. IL-6 and stat3 are required for survival of intestinal epithelial cells and development of colitis-associated cancer. Cancer Cell 15(2):103-13. [PubMed: 19185845] [MGI Ref ID J:144813]
Guilmeau S; Flandez M; Bancroft L; Sellers RS; Tear B; Stanley P; Augenlicht LH. 2008. Intestinal deletion of Pofut1 in the mouse inactivates notch signaling and causes enterocolitis. Gastroenterology 135(3):849-60, 860.e1-6. [PubMed: 18621050] [MGI Ref ID J:140424]
Harmon GS; Dumlao DS; Ng DT; Barrett KE; Dennis EA; Dong H; Glass CK. 2010. Pharmacological correction of a defect in PPAR-gamma signaling ameliorates disease severity in Cftr-deficient mice. Nat Med 16(3):313-8. [PubMed: 20154695] [MGI Ref ID J:158591]
Herbert DR; Yang JQ; Hogan SP; Groschwitz K; Khodoun M; Munitz A; Orekov T; Perkins C; Wang Q; Brombacher F; Urban JF Jr; Rothenberg ME; Finkelman FD. 2009. Intestinal epithelial cell secretion of RELM-beta protects against gastrointestinal worm infection. J Exp Med 206(13):2947-57. [PubMed: 19995957] [MGI Ref ID J:155675]
Hinoi T; Akyol A; Theisen BK; Ferguson DO; Greenson JK; Williams BO; Cho KR; Fearon ER. 2007. Mouse model of colonic adenoma-carcinoma progression based on somatic Apc inactivation. Cancer Res 67(20):9721-30. [PubMed: 17942902] [MGI Ref ID J:126018]
Ianari A; Natale T; Calo E; Ferretti E; Alesse E; Screpanti I; Haigis K; Gulino A; Lees JA. 2009. Proapoptotic function of the retinoblastoma tumor suppressor protein. Cancer Cell 15(3):184-94. [PubMed: 19249677] [MGI Ref ID J:145939]
Iqbal J; Li X; Chang BH; Chan L; Schwartz GJ; Chua SC Jr; Hussain MM. 2010. An intrinsic gut leptin-melanocortin pathway modulates intestinal microsomal triglyceride transfer protein and lipid absorption. J Lipid Res 51(7):1929-42. [PubMed: 20164094] [MGI Ref ID J:162835]
Ishikawa TO; Herschman HR. 2010. Conditional Bicistronic Cre Reporter Line Expressing Both Firefly Luciferase and beta-galactosidase. Mol Imaging Biol :. [PubMed: 20495880] [MGI Ref ID J:165837]
Ishikawa TO; Oshima M; Herschman HR. 2011. Cox-2 deletion in myeloid and endothelial cells, but not in epithelial cells, exacerbates murine colitis. Carcinogenesis 32(3):417-26. [PubMed: 21156970] [MGI Ref ID J:169324]
Jones RG; Li X; Gray PD; Kuang J; Clayton F; Samowitz WS; Madison BB; Gumucio DL; Kuwada SK. 2006. Conditional deletion of beta1 integrins in the intestinal epithelium causes a loss of Hedgehog expression, intestinal hyperplasia, and early postnatal lethality. J Cell Biol 175(3):505-14. [PubMed: 17088430] [MGI Ref ID J:119155]
Kajino-Sakamoto R; Inagaki M; Lippert E; Akira S; Robine S; Matsumoto K; Jobin C; Ninomiya-Tsuji J. 2008. Enterocyte-derived TAK1 signaling prevents epithelium apoptosis and the development of ileitis and colitis. J Immunol 181(2):1143-52. [PubMed: 18606667] [MGI Ref ID J:137419]
Kajino-Sakamoto R; Omori E; Nighot PK; Blikslager AT; Matsumoto K; Ninomiya-Tsuji J. 2010. TGF-beta-activated kinase 1 signaling maintains intestinal integrity by preventing accumulation of reactive oxygen species in the intestinal epithelium. J Immunol 185(8):4729-37. [PubMed: 20855879] [MGI Ref ID J:164727]
Kang YJ; Otsuka M; van den Berg A; Hong L; Huang Z; Wu X; Zhang DW; Vallance BA; Tobias PS; Han J. 2010. Epithelial p38alpha controls immune cell recruitment in the colonic mucosa. PLoS Pathog 6(6):e1000934. [PubMed: 20532209] [MGI Ref ID J:162332]
Kawaguchi M; Takeda N; Hoshiko S; Yorita K; Baba T; Sawaguchi A; Nezu Y; Yoshikawa T; Fukushima T; Kataoka H. 2011. Membrane-bound serine protease inhibitor HAI-1 is required for maintenance of intestinal epithelial integrity. Am J Pathol 179(4):1815-26. [PubMed: 21840293] [MGI Ref ID J:179937]
Kim I; Ahn SH; Inagaki T; Choi M; Ito S; Guo GL; Kliewer SA; Gonzalez FJ. 2007. Differential regulation of bile acid homeostasis by the farnesoid X receptor in liver and intestine. J Lipid Res 48(12):2664-72. [PubMed: 17720959] [MGI Ref ID J:129978]
Kim JY; Kajino-Sakamoto R; Omori E; Jobin C; Ninomiya-Tsuji J. 2009. Intestinal epithelial-derived TAK1 signaling is essential for cytoprotection against chemical-induced colitis. PLoS ONE 4(2):e4561. [PubMed: 19234607] [MGI Ref ID J:146227]
Kwon MC; Koo BK; Kim YY; Lee SH; Kim NS; Kim JH; Kong YY. 2009. Essential role of CR6-interacting factor 1 (Crif1) in E74-like factor 3 (ELF3)-mediated intestinal development. J Biol Chem 284(48):33634-41. [PubMed: 19801644] [MGI Ref ID J:156319]
Langlois MJ; Roy SA; Auclair BA; Jones C; Boudreau F; Carrier JC; Rivard N; Perreault N. 2009. Epithelial phosphatase and tensin homolog regulates intestinal architecture and secretory cell commitment and acts as a modifier gene in neoplasia. FASEB J 23(6):1835-44. [PubMed: 19168705] [MGI Ref ID J:150553]
Lee D; Yu M; Lee E; Kim H; Yang Y; Kim K; Pannicia C; Kurie JM; Threadgill DW. 2009. Tumor-specific apoptosis caused by deletion of the ERBB3 pseudo-kinase in mouse intestinal epithelium. J Clin Invest 119(9):2702-13. [PubMed: 19690388] [MGI Ref ID J:152703]
Lee YK; Schmidt DR; Cummins CL; Choi M; Peng L; Zhang Y; Goodwin B; Hammer RE; Mangelsdorf DJ; Kliewer SA. 2008. Liver receptor homolog-1 regulates bile Acid homeostasis but is not essential for feedback regulation of bile Acid synthesis. Mol Endocrinol 22(6):1345-56. [PubMed: 18323469] [MGI Ref ID J:135822]
List K; Kosa P; Szabo R; Bey AL; Wang CB; Molinolo A; Bugge TH. 2009. Epithelial integrity is maintained by a matriptase-dependent proteolytic pathway. Am J Pathol 175(4):1453-63. [PubMed: 19717635] [MGI Ref ID J:153070]
Liu JY; Seno H; Miletic AV; Mills JC; Swat W; Stappenbeck TS. 2009. Vav proteins are necessary for correct differentiation of mouse cecal and colonic enterocytes. J Cell Sci 122(Pt 3):324-34. [PubMed: 19139088] [MGI Ref ID J:146174]
Lozovatsky L; Abayasekara N; Piawah S; Walther Z. 2009. CASK deletion in intestinal epithelia causes mislocalization of LIN7C and the DLG1/Scrib polarity complex without affecting cell polarity. Mol Biol Cell 20(21):4489-99. [PubMed: 19726564] [MGI Ref ID J:164479]
Luebke-Wheeler J; Zhang K; Battle M; Si-Tayeb K; Garrison W; Chhinder S; Li J; Kaufman RJ; Duncan SA. 2008. Hepatocyte nuclear factor 4alpha is implicated in endoplasmic reticulum stress-induced acute phase response by regulating expression of cyclic adenosine monophosphate responsive element binding protein H. Hepatology 48(4):1242-50. [PubMed: 18704925] [MGI Ref ID J:140018]
Mensah-Osman E; Zavros Y; Merchant JL. 2008. Somatostatin stimulates menin gene expression by inhibiting protein kinase A. Am J Physiol Gastrointest Liver Physiol 295(4):G843-54. [PubMed: 18755809] [MGI Ref ID J:142454]
Mori-Akiyama Y; van den Born M; van Es JH; Hamilton SR; Adams HP; Zhang J; Clevers H; de Crombrugghe B. 2007. SOX9 is required for the differentiation of paneth cells in the intestinal epithelium. Gastroenterology 133(2):539-46. [PubMed: 17681175] [MGI Ref ID J:128277]
Mortier E; Advincula R; Kim L; Chmura S; Barrera J; Reizis B; Malynn BA; Ma A. 2009. Macrophage- and dendritic-cell-derived interleukin-15 receptor alpha supports homeostasis of distinct CD8+ T cell subsets. Immunity 31(5):811-22. [PubMed: 19913445] [MGI Ref ID J:155298]
Munera J; Cecena G; Jedlicka P; Wankell M; Oshima RG. 2011. Ets2 regulates colonic stem cells and sensitivity to tumorigenesis. Stem Cells 29(3):430-9. [PubMed: 21425406] [MGI Ref ID J:171112]
Munoz NM; Upton M; Rojas A; Washington MK; Lin L; Chytil A; Sozmen EG; Madison BB; Pozzi A; Moon RT; Moses HL; Grady WM. 2006. Transforming growth factor beta receptor type II inactivation induces the malignant transformation of intestinal neoplasms initiated by Apc mutation. Cancer Res 66(20):9837-44. [PubMed: 17047044] [MGI Ref ID J:114960]
Murray NR; Weems J; Braun U; Leitges M; Fields AP. 2009. Protein kinase C betaII and PKCiota/lambda: collaborating partners in colon cancer promotion and progression. Cancer Res 69(2):656-62. [PubMed: 19147581] [MGI Ref ID J:143707]
Nemere I; Garbi N; Hammerling GJ; Khanal RC. 2010. Intestinal cell calcium uptake and the targeted knockout of the 1,25D3-MARRS (membrane-associated, rapid response steroid-binding) receptor/PDIA3/Erp57. J Biol Chem 285(41):31859-66. [PubMed: 20682787] [MGI Ref ID J:166800]
Nenci A; Becker C; Wullaert A; Gareus R; van Loo G; Danese S; Huth M; Nikolaev A; Neufert C; Madison B; Gumucio D; Neurath MF; Pasparakis M. 2007. Epithelial NEMO links innate immunity to chronic intestinal inflammation. Nature 446(7135):557-61. [PubMed: 17361131] [MGI Ref ID J:120854]
Noah TK; Kazanjian A; Whitsett J; Shroyer NF. 2010. SAM pointed domain ETS factor (SPDEF) regulates terminal differentiation and maturation of intestinal goblet cells. Exp Cell Res 316(3):452-65. [PubMed: 19786015] [MGI Ref ID J:156638]
Nose Y; Kim BE; Thiele DJ. 2006. Ctr1 drives intestinal copper absorption and is essential for growth, iron metabolism, and neonatal cardiac function. Cell Metab 4(3):235-44. [PubMed: 16950140] [MGI Ref ID J:129741]
Nose Y; Wood LK; Kim BE; Prohaska JR; Fry RS; Spears JW; Thiele DJ. 2010. Ctr1 is an apical copper transporter in mammalian intestinal epithelial cells in vivo that is controlled at the level of protein stability. J Biol Chem 285(42):32385-92. [PubMed: 20699218] [MGI Ref ID J:166794]
Owen KA; Abshire MY; Tilghman RW; Casanova JE; Bouton AH. 2011. FAK Regulates Intestinal Epithelial Cell Survival and Proliferation during Mucosal Wound Healing. PLoS One 6(8):e23123. [PubMed: 21887232] [MGI Ref ID J:176155]
Patankar JV; Chandak PG; Obrowsky S; Pfeifer T; Diwoky C; Uellen A; Sattler W; Stollberger R; Hoefler G; Heinemann A; Battle M; Duncan S; Kratky D; Levak-Frank S. 2011. Loss of intestinal GATA4 prevents diet-induced obesity and promotes insulin sensitivity in mice. Am J Physiol Endocrinol Metab 300(3):E478-88. [PubMed: 21177287] [MGI Ref ID J:172316]
Petrova TV; Nykanen A; Norrmen C; Ivanov KI; Andersson LC; Haglund C; Puolakkainen P; Wempe F; von Melchner H; Gradwohl G; Vanharanta S; Aaltonen LA; Saharinen J; Gentile M; Clarke A; Taipale J; Oliver G; Alitalo K. 2008. Transcription factor PROX1 induces colon cancer progression by promoting the transition from benign to highly dysplastic phenotype. Cancer Cell 13(5):407-19. [PubMed: 18455124] [MGI Ref ID J:134772]
Pickert G; Neufert C; Leppkes M; Zheng Y; Wittkopf N; Warntjen M; Lehr HA; Hirth S; Weigmann B; Wirtz S; Ouyang W; Neurath MF; Becker C. 2009. STAT3 links IL-22 signaling in intestinal epithelial cells to mucosal wound healing. J Exp Med 206(7):1465-72. [PubMed: 19564350] [MGI Ref ID J:150265]
Qiao XT; Ziel JW; McKimpson W; Madison BB; Todisco A; Merchant JL; Samuelson LC; Gumucio DL. 2007. Prospective identification of a multilineage progenitor in murine stomach epithelium. Gastroenterology 133(6):1989-98. [PubMed: 18054570] [MGI Ref ID J:135613]
Rigby RJ; Simmons JG; Greenhalgh CJ; Alexander WS; Lund PK. 2007. Suppressor of cytokine signaling 3 (SOCS3) limits damage-induced crypt hyper-proliferation and inflammation-associated tumorigenesis in the colon. Oncogene 26(33):4833-41. [PubMed: 17297444] [MGI Ref ID J:125546]
Roulis M; Armaka M; Manoloukos M; Apostolaki M; Kollias G. 2011. Intestinal epithelial cells as producers but not targets of chronic TNF suffice to cause murine Crohn-like pathology. Proc Natl Acad Sci U S A 108(13):5396-401. [PubMed: 21402942] [MGI Ref ID J:171234]
Sato T; Mushiake S; Kato Y; Sato K; Sato M; Takeda N; Ozono K; Miki K; Kubo Y; Tsuji A; Harada R; Harada A. 2007. The Rab8 GTPase regulates apical protein localization in intestinal cells. Nature 448(7151):366-9. [PubMed: 17597763] [MGI Ref ID J:123415]
Shroyer NF; Helmrath MA; Wang VY; Antalffy B; Henning SJ; Zoghbi HY. 2007. Intestine-specific ablation of mouse atonal homolog 1 (Math1) reveals a role in cellular homeostasis. Gastroenterology 132(7):2478-88. [PubMed: 17570220] [MGI Ref ID J:128312]
Smalley-Freed WG; Efimov A; Burnett PE; Short SP; Davis MA; Gumucio DL; Washington MK; Coffey RJ; Reynolds AB. 2010. p120-catenin is essential for maintenance of barrier function and intestinal homeostasis in mice. J Clin Invest 120(6):1824-35. [PubMed: 20484816] [MGI Ref ID J:161638]
Sodhi CP; Li J; Duncan SA. 2006. Generation of mice harbouring a conditional loss-of-function allele of Gata6. BMC Dev Biol 6:19. [PubMed: 16611361] [MGI Ref ID J:111388]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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 |
Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(Vil-cre)997Gum
Pairs /Price (US dollars $) Pair Genotype $280.40 C57BL/6J (000664) x Hemizygous for Tg(Vil-cre)997Gum Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
|
Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(Vil-cre)997Gum
Pairs /Price (US dollars $) Pair Genotype $364.60 C57BL/6J (000664) x Hemizygous for Tg(Vil-cre)997Gum Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
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Tel: 1-800-422-6423 or 1-207-288-5845
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For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiriesContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.