Strain Name: |
B6.SJL-Tg(Vil-cre)997Gum/J |
|---|---|
Stock Number: |
004586 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6;SJL-Tg(Vil-cre)997Gum (Changed: 15-DEC-04
) |
| Genes & Alleles | Vill; cre; Tg(Vil-cre)997Gum; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Inbred x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Deborah Gumucio, University of Michigan Generation N5+12 (06-DEC-07) Strain Description
Mice hemizygous for this transgene express Cre recombinase under the direction of the mouse villin 1 promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in villi and crypt cells of the small and large intestines, closely patterning the endogenous gene expression. The Donating Investigator indicates that expression is generally continuous, but that a small amount of mosaicism is noted in the colon. Onset of transgene expression is at 12.5 dpc, which is delayed from the endogenous mouse Vil1 gene expression onset of 9.0 dpc. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of intestinal organogenesis.Strain Development
A transgenic construct containing the coding domain of Cre recombinase, driven by a 12.4 kb fragment of the mouse villin 1 gene, and a metallothionein polyadenylation site sequence was injected into the pronuclei of fertilized C57BL/6J X SJL/J mouse eggs. Founder animals were bred to C57BL/6 mice and then backcrossed to the same for 5 generations.
Gene & Allele Details
| Allele Symbol | Tg(Vil-cre)997Gum | ||
|---|---|---|---|
| Allele Name | transgene insertion 997, Deborah L Gumucio | ||
| Common Name(s) | 12.4KbVilCre; Villin-cre; | ||
| Mutation Made By | Blair Madison, University of Michigan | ||
| Strain of Origin | (C57BL/6J x SJL)F1 | ||
| Site of Expression | epithelial cells of the small and large intestines; onset of expression is at 12.5 d.p.c.; expression is generally continuous, but a small amount of mosaicism is noted in the colon | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Vill, villin-like, mouse, laboratory | ||
| General Note | Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. | ||
| Molecular Note | A 12.4-kb promoter fragment from the villin gene drives the expression of Cre recombinase. Recombination occurs in villi and crypt cells of the small and large intestines, closely patterning the endogenous gene expression. According to the corresponding author (J:78815), expression is generally continuous but a small amount of mosaicism is noted in the colon. Onset of transgene expression is at 12.5 dpc, which is delayed from the endogenous mouse Vil gene expression onset of 9.0 dpc. [MGI Ref ID J:78815] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Vil-cre)997Gum
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (115 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Developmental Biology Research (Cre-lox System)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesMadison BB; Dunbar L; Qiao XT; Braunstein K; Braunstein E; Gumucio DL. 2002. cis Elements of the Villin Gene Control Expression in Restricted Domains of the Vertical (Crypt) and Horizontal (Duodenum, Cecum) Axes of the Intestine. J Biol Chem 277(36):33275-83. [PubMed: 12065599] [MGI Ref ID J:78815]
Price and Supply Information
| Strain Name: | B6.SJL-Tg(Vil-cre)997Gum/J |
| Stock Number: | 004586 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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