Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6Ei.Cg-TWis    (Changed: 15-DEC-04 ) B6Ei.Cg-TWis/Ei    (Changed: 15-DEC-04 ) Type Congenic; Mutant Stock; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N10p

Related Strains

Strains carrying other alleles of T

003879	B10;TFLe-a/a T tf/+ tf/J
000405	B10ScSn.Cg-T/J
000953	B6 x BALB/cBy-T4J/J
001518	B6 x STOCK T tf/th45 tf/J
000567	B6.Cg-T2J +/+ Qkqk/J
001015	B6.Cg-T4Or/J
001054	B6.Cg-TOrl/EiJ
000350	B6By.Cg-KitW-v MitfMi-wh T/J
002282	BTBR T+ tf/J
001053	C3Sn.AK-Thp/EiJ
000545	C57BL/6J-T2J/J
001199	C57BL/6J-T5J/J
001961	C57BL/6JEi x STOCK T T(16;17)43H/+ T(16;17)43H/Ei
001814	STOCK Tc/J

View Strains carrying other alleles of T     (14 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

T^Wis/T⁺

        involves: A/J

• limbs/digits/tail phenotype
• absent tail (MGI Ref ID J:9032)

T^Wis/T^Wis

        Background Not Specified

• lethality-prenatal/perinatal
• embryonic lethality before turning of embryo (MGI Ref ID J:78124)
• embryogenesis phenotype
• abnormal axial mesoderm (MGI Ref ID J:78124)
  • formation is disrupted
• abnormal notochord morphology (MGI Ref ID J:78124)
  • at E8 extension of the notochord precursor is disrupted
• abnormal primitive streak formation (MGI Ref ID J:78124)
• absent allantois (MGI Ref ID J:78124)

T^Wis/T^Wis

        involves: A/J

• lethality-prenatal/perinatal
• embryonic lethality (MGI Ref ID J:9032)

Genes & Alleles

Gene & Allele Information

Allele Symbol		TWis
Allele Name		brachyury Wisconsin
Allele Type		Spontaneous
Strain of Origin		A/J
Gene Symbol and Name		T, brachyury
Chromosome		17
Gene Common Name(s)		Bra; Low; Lr; MGC104817; T1; TFT; Tl2; Tl3; brachyury-like 2; brachyury-like 3; cou; coupe; low ratio; me75;
Molecular Note		Restriction mapping showed that this allele comprises a 5.5 kb early transposon-like insertion element. RT-PCR based analysis shows that at least 8 different mutant transcripts are produced from the TWis allele. These transcripts bypass the mutated exon 7 splice donor site and no wild type T transcripts are produced. [MGI Ref ID J:107619] [MGI Ref ID J:21263]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Additional References

T^Wis related

Beddington RS; Rashbass P; Wilson V. 1992. Brachyury--a gene affecting mouse gastrulation and early organogenesis. Dev Suppl :157-65. [PubMed: 1299362]  [MGI Ref ID J:4153]

Chapman DL; Cooper-Morgan A; Harrelson Z; Papaioannou VE. 2003. Critical role for Tbx6 in mesoderm specification in the mouse embryo. Mech Dev 120(7):837-47. [PubMed: 12915233]  [MGI Ref ID J:85153]

Conlon FL; Wright CV; Robertson EJ. 1995. Effects of the TWis mutation on notochord formation and mesodermal patterning. Mech Dev 49(3):201-9. [PubMed: 7734393]  [MGI Ref ID J:23131]

Goldin SN; Papaioannou VE. 2003. Unusual misregulation of RNA splicing caused by insertion of a transposable element into the T (Brachyury) locus. BMC Genomics 4(1):14. [PubMed: 12702217]  [MGI Ref ID J:107619]

Herrmann BG. 1991. Expression pattern of the Brachyury gene in whole-mount TWis/TWis mutant embryos. Development 113(3):913-7. [PubMed: 1821859]  [MGI Ref ID J:1975]

Herrmann BG; Labeit S; Poustka A; King TR; Lehrach H. 1990. Cloning of the T gene required in mesoderm formation in the mouse [see comments] Nature 343(6259):617-22. [PubMed: 2154694]  [MGI Ref ID J:21263]

Shedlovsky A; King TR; Dove WF. 1988. Saturation germ line mutagenesis of the murine t region including a lethal allele at the quaking locus. Proc Natl Acad Sci U S A 85(1):180-4. [PubMed: 3422415]  [MGI Ref ID J:9032]

Yamaguchi TP; Takada S; Yoshikawa Y; Wu N; McMahon AP. 1999. T (Brachyury) is a direct target of Wnt3a during paraxial mesoderm specification. Genes Dev 13(24):3185-90. [PubMed: 10617567]  [MGI Ref ID J:59271]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). Genomic DNA is available for this strain from the Mouse DNA Resource.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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