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Former Names B6Ei.Cg-TWis (Changed: 15-DEC-04 ) B6Ei.Cg-TWis/Ei (Changed: 15-DEC-04 ) Type Congenic; Mutant Stock; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N10p
Strains carrying other alleles of T
003879 B10;TFLe-a/a T tf/+ tf/J 000405 B10ScSn.Cg-T/J 000953 B6 x BALB/cBy-T4J/J 001518 B6 x STOCK T tf/th45 tf/J 000567 B6.Cg-T2J +/+ Qkqk/J 001015 B6.Cg-T4Or/J 001054 B6.Cg-TOrl/EiJ 000350 B6By.Cg-KitW-v MitfMi-wh T/J 002282 BTBR T+ tf/J 001053 C3Sn.AK-Thp/EiJ 000545 C57BL/6J-T2J/J 001199 C57BL/6J-T5J/J 001961 C57BL/6JEi x STOCK T T(16;17)43H/+ T(16;17)43H/Ei 001814 STOCK Tc/J View Strains carrying other alleles of T (14 strains)
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
TWis/T+
involves: A/J
- limbs/digits/tail phenotype
- absent tail (MGI Ref ID J:9032)
TWis/TWis
Background Not Specified
- lethality-prenatal/perinatal
- embryonic lethality before turning of embryo (MGI Ref ID J:78124)
- embryogenesis phenotype
- abnormal axial mesoderm (MGI Ref ID J:78124)
- formation is disrupted
- abnormal notochord morphology (MGI Ref ID J:78124)
- at E8 extension of the notochord precursor is disrupted
- abnormal primitive streak formation (MGI Ref ID J:78124)
- absent allantois (MGI Ref ID J:78124)
TWis/TWis
involves: A/J
- lethality-prenatal/perinatal
- embryonic lethality (MGI Ref ID J:9032)
| Allele Symbol | TWis | ||
|---|---|---|---|
| Allele Name | brachyury Wisconsin | ||
| Allele Type | Spontaneous | ||
| Strain of Origin | A/J | ||
| Gene Symbol and Name | T, brachyury | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | Bra; Low; Lr; MGC104817; T1; TFT; Tl2; Tl3; brachyury-like 2; brachyury-like 3; cou; coupe; low ratio; me75; | ||
| Molecular Note | Restriction mapping showed that this allele comprises a 5.5 kb early transposon-like insertion element. RT-PCR based analysis shows that at least 8 different mutant transcripts are produced from the TWis allele. These transcripts bypass the mutated exon 7 splice donor site and no wild type T transcripts are produced. [MGI Ref ID J:107619] [MGI Ref ID J:21263] | ||
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
TWis relatedBeddington RS; Rashbass P; Wilson V. 1992. Brachyury--a gene affecting mouse gastrulation and early organogenesis. Dev Suppl :157-65. [PubMed: 1299362] [MGI Ref ID J:4153]
Chapman DL; Cooper-Morgan A; Harrelson Z; Papaioannou VE. 2003. Critical role for Tbx6 in mesoderm specification in the mouse embryo. Mech Dev 120(7):837-47. [PubMed: 12915233] [MGI Ref ID J:85153]
Conlon FL; Wright CV; Robertson EJ. 1995. Effects of the TWis mutation on notochord formation and mesodermal patterning. Mech Dev 49(3):201-9. [PubMed: 7734393] [MGI Ref ID J:23131]
Goldin SN; Papaioannou VE. 2003. Unusual misregulation of RNA splicing caused by insertion of a transposable element into the T (Brachyury) locus. BMC Genomics 4(1):14. [PubMed: 12702217] [MGI Ref ID J:107619]
Herrmann BG. 1991. Expression pattern of the Brachyury gene in whole-mount TWis/TWis mutant embryos. Development 113(3):913-7. [PubMed: 1821859] [MGI Ref ID J:1975]
Herrmann BG; Labeit S; Poustka A; King TR; Lehrach H. 1990. Cloning of the T gene required in mesoderm formation in the mouse [see comments] Nature 343(6259):617-22. [PubMed: 2154694] [MGI Ref ID J:21263]
Shedlovsky A; King TR; Dove WF. 1988. Saturation germ line mutagenesis of the murine t region including a lethal allele at the quaking locus. Proc Natl Acad Sci U S A 85(1):180-4. [PubMed: 3422415] [MGI Ref ID J:9032]
Yamaguchi TP; Takada S; Yoshikawa Y; Wu N; McMahon AP. 1999. T (Brachyury) is a direct target of Wnt3a during paraxial mesoderm specification. Genes Dev 13(24):3185-90. [PubMed: 10617567] [MGI Ref ID J:59271]
Currently there no information available for this strain. This may be due to the supply level of this strain.
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Purchasing Information
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