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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6;129S4-Gt(ROSA)26Sortm1(ALPP)Dym/J (Changed: 12-JAN-05 ) Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Donating Investigator Susan Dymecki, Harvard Medical School Description
These mice possess a disrupted human placental alkaline phosphatase (ALPP) transgene inserted into the Gt(ROSA)26Sor locus. Expression of the ALPP transgene is contingent upon Flp-mediated excision of the FRT-flanked STOP cassette placed between the ALPP peptide encoding sequence and the Gt(ROSA)26Sor promoter. This mutant strain serves as a reporter strain, with successful Flp recombinase-mediated excision indicated by ALPP activity. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.When bred to a strain with broad expression of FLP1 recombinase (see Stock No. 003946 for example), this mutant mouse strain may be useful as an indicator strain for marking most cells in the developing and adult mouse.
Development
A FRT-flanked targeting vector containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter and four head-to-tail SV40 polyadenylation sites was used to disrupt the human placental alkaline phosphatase (ALPP) transgene. This FRT-disrupted ALPP transgene was then inserted into the Gt(ROSA)26Sor locus, disrupting expression of the Gt(ROSA)26Sor gene. The final construct, R26FRAPki, was electroporated into 129S4/SvJaeSor derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (46 strains)
Additional Web Information
FLP-FRT Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Gt(ROSA)26Sortm1(FLP1)Dym/Gt(ROSA)26Sortm2Dym
involves: 129S4/SvJaeSor * C57BL/6J (conditional)
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:72406)
- mice show widespread human placental alkaline phosphatase activity
This mouse can be used to support research in many areas including:
Research Tools
FLP-FRT System (FRT-flanked Sequences: Test/Reporter)
Genetics Research (Tissue/Cell Markers)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm2Dym | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Susan Dymecki | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Gt(ROSA)26Sortm1(ALPP)Dym; R26:FRAP; R26FRAPki; | ||
| Mutation Made By | Susan Dymecki, Harvard Medical School | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | when crossed to a FLP recombinase-expressing strain, expression of human placental alkaline phosphatase (ALPP) is observed in the FLP-expressing tissues | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | An Flp-dependent alkaline phosphatase reporter locus was generated by inserting a construct containing an frt-flanked PGK-neo gene followed by sequence encoding human ALPP. The inserted construct contained a splice acceptor site at the 5' end. In addition to transcriptional and translational stop signals in the frt-flanked region, the frt-flanked neo cassette was inserted immediately downstream of the ALPP start codon to ensure a lack of ALPP activity in the absence of Flp-mediated excision. [MGI Ref ID J:72406] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm2Dym, STD PCR, vers. 2
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Awatramani R; Soriano P; Mai JJ; Dymecki S. 2001. An Flp indicator mouse expressing alkaline phosphatase from the ROSA26 locus. Nat Genet 29(3):257-9. [PubMed: 11687793] [MGI Ref ID J:72406]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry The resulting chimeric animals were bred to produce homozygous mice. When maintained in a live colony, this strain may be bred using homozygous matings. Expected coat color is: Black and agouti. Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00 Cryopreserved Embryos Fee $1600.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00 Cryopreserved Embryos Fee $2080.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Terms of Use
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General Terms and Conditions
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Contact information
General inquiries
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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