Strain Name: |
FVB-Tg(GFAP-cre)25Mes/J |
|---|---|
Stock Number: |
004600 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
FVB-Tg(GFAP-cre)25Mes (Changed: 15-DEC-04
) |
| Genes & Alleles | GFAP; cre; Tg(GFAP-cre)25Mes; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Hemizygote x Inbred (Female x Male) Species laboratory mouse Donating Investigator Albee Messing, University of Wisconsin-Madison Generation (N?+1p)N11 (08-JAN-08) Strain Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Mice that are homozygous for the transgene are not viable. This transgenic mouse strain expresses Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP). When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons. Expression activity is also present in periportal cells of the liver. Developmental onset of transgene expression occurs in the dorsal and medial regions of the telencephalon by embryonic day 13.5. In adult cerebellum, only astrocytes are immunoreactive for GFAP or Cre recombinase. This mutant mouse strain represents an effective tool for generating central nervous system specific-targeted mutants.Strain Development
A transgenic construct containing a 2.2Kb 5' flanking region of the human GFAP gene, cre coding sequence, the SV40 nuclear localization signal, and a portion of the mouse protamine (Prm1) gene which supplies intronic sequence and a polyadenylation site, was injected into fertilized FVB/N mouse eggs.
Gene & Allele Details
| Allele Symbol | Tg(GFAP-cre)25Mes | ||
|---|---|---|---|
| Allele Name | transgene insertion 25, Albee Messing | ||
| Common Name(s) | GFAP-Cre; GFAP-Cre-m; Tg95.2; TgN(GFAPcre)25Mes; TgN25Mes; hGFAP-cre; hGFAP::Cre; | ||
| Mutation Made By | Albee Messing, University of Wisconsin-Madison | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons; also periportal cells of the liver | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | GFAP, glial fibrillary acidic protein, human | ||
| General Note | Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Mice that are homozygous for the transgene are not viable. | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a human glial fibrillary acidic protein (GFAP) promoter. Cre-mediated recombination occurs primarily in the central nervous system, affecting astrocytes, oligodendroglia, ependyma and some neurons. Expression activity is also present in periportal cells of the liver. Developmental onset of transgene expression occurs in the dorsal and medial regions of the telencephalon by embryonic day 13.5. In adult cerebellum, only astrocytes are immunoreactive for GFAP or Cre recombinase. [J:72509] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(GFAP-cre)25Mes
Colony Maintenance
| Breeding & Husbandry | The strain is maintained as a hemizygote on the FVB/N background. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of GFAP
View Strains carrying other alleles of GFAP (7 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Genetic Quality Control Annual Report
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System (Cre Recombinase expression in neural tissue)
Research Tools
Cre-lox-System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
Neurobiology Research
Research Tools
Cre-lox-System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
References
Selected Reference(s)
Additional ReferencesZhuo L; Theis M; Alvarez-Maya I; Brenner M; Willecke K; Messing A. 2001. hGFAP-cre transgenic mice for manipulation of glial and neuronal function in vivo. Genesis 31(2):85-94. [PubMed: 11668683] [J:72509]
Price and Supply Information
| Strain Name: | FVB-Tg(GFAP-cre)25Mes/J |
| Stock Number: | 004600 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
*NO Shipping Destination selected!
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form