Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6;129-Catna1tm1Efu/J    (Changed: 02-JUN-05 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?+F3+N1p (31-JUL-05) Generation Definitions   Donating Investigator Elaine Fuchs,   The Rockefeller University

Description
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain expressing Cre recombinase in the female germline (see Stock No. 003551 for example), this mutant mouse strain may be useful in studies of mammary epithelium.

When bred to a strain expressing Cre recombinase in the mammary gland (see Stock No. 003552 for example), this mutant mouse strain may be useful in studies of mammary epithelium.

When bred to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771 for example), this mutant mouse strain may be useful in studies of the cerebral cortex and the hedgehog signalling pathway.

Development
A loxP site flanked targeting vector containing a neomycin resistance gene was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 2 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into 129/Sv derived GS1 embryonic stem (ES) cells which were transiently transfected with a vector containing the Cre-recombinase gene under the control of the cytomegalovirus promoter to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6J blastocysts.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Ctnna1^tm1Efu/Ctnna1^tm1Efu

        involves: 129X1/SvJ

• integument phenotype
• abnormal skin physiology
  • mice exhibit increased apoptosis in the epidermis compared to wild-type mice   (MGI Ref ID J:144091)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX^® Services jaxservices@jax.org for customized breeding options.

Ctnna1^tm1Efu/Ctnna1^tm1Efu Tg(MMTV-cre)4Mam/?

        involves: 129X1/SvJ * FVB   (conditional)

• endocrine/exocrine gland phenotype
• abnormal mammary gland epithelium morphology
  • mammary glands have reduced numbers of epithelial cells   (MGI Ref ID J:87469)
  • some epithelial cells remain condensed and disorganized without forming alveolar structures   (MGI Ref ID J:87469)
  • lumens are absent from clusters of epithelium   (MGI Ref ID J:87469)
• abnormal mammary gland epithelium physiology
  • apoptosis in mammary epithelium is increased   (MGI Ref ID J:87469)
• abnormal milk composition
  • milk proteins are reduced   (MGI Ref ID J:87469)
• integument phenotype
• abnormal hair follicle development
  • occasionally hair development is not on schedule   (MGI Ref ID J:87469)
• abnormal mammary gland epithelium morphology
  • mammary glands have reduced numbers of epithelial cells   (MGI Ref ID J:87469)
  • some epithelial cells remain condensed and disorganized without forming alveolar structures   (MGI Ref ID J:87469)
  • lumens are absent from clusters of epithelium   (MGI Ref ID J:87469)
• abnormal mammary gland epithelium physiology
  • apoptosis in mammary epithelium is increased   (MGI Ref ID J:87469)
• abnormal milk composition
  • milk proteins are reduced   (MGI Ref ID J:87469)

Ctnna1^tm1Efu/Ctnna1^tm1Efu Tg(Wap-cre)11738Mam/?

        involves: 129X1/SvJ * C57BL/6 * SJL   (conditional)

• growth/size phenotype
• decreased body weight
  • pups with homozygous mothers weight 50% of normal mice   (MGI Ref ID J:87469)
• postnatal growth retardation
  • pups with homozygous mothers fail to thrive   (MGI Ref ID J:87469)
• endocrine/exocrine gland phenotype
• abnormal mammary gland epithelium morphology
  • mammary glands have reduced numbers of epithelial cells   (MGI Ref ID J:87469)
  • some epithelial cells remain condensed and disorganized without forming alveolar structures   (MGI Ref ID J:87469)
  • lumens are absent from clusters of epithelium   (MGI Ref ID J:87469)
  • apoptosis in mammary epithelium is increased   (MGI Ref ID J:87469)
• abnormal milk composition
  • milk proteins are reduced   (MGI Ref ID J:87469)
• other phenotype
• maternal effect
  • pups with homozygous mothers fail to thrive, are about 50% of normal weight and some do not survive lactation   (MGI Ref ID J:87469)
  • in litters that experience lethality, the litters are reduced to 50% at day 5 and 25% by day 10   (MGI Ref ID J:87469)
• integument phenotype
• abnormal mammary gland epithelium morphology
  • mammary glands have reduced numbers of epithelial cells   (MGI Ref ID J:87469)
  • some epithelial cells remain condensed and disorganized without forming alveolar structures   (MGI Ref ID J:87469)
  • lumens are absent from clusters of epithelium   (MGI Ref ID J:87469)
  • apoptosis in mammary epithelium is increased   (MGI Ref ID J:87469)
• abnormal milk composition
  • milk proteins are reduced   (MGI Ref ID J:87469)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Cre-lox System

Ctnna1^tm1Efu related

Cell Biology Research
Defects in Cell Adhesion Molecules

Developmental Biology Research
Defects in Cell Adhesion Molecules

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Ctnna1tm1Efu
Allele Name		targeted mutation 1, Elaine Fuchs
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		alpha-cat fl; floxed alpha-E-catenin;
Strain of Origin		129X1/SvJ
ES Cell Line Name		RW-4
ES Cell Line Strain		129X1/SvJ
Gene Symbol and Name		Ctnna1, catenin (cadherin associated protein), alpha 1
Chromosome		18
Gene Common Name(s)		2010010M04Rik; AA517462; AI988031; CAP102; Catna1; RIKEN cDNA 2010010M04 gene; alpha E catenin; alpha(E)-catenin; catenin alpha 1; expressed sequence AA517462; expressed sequence AI988031;
Molecular Note		LoxP sites were inserted by homologous recombination that flanked exon 2. An adjacent floxed neomycin selection cassette was removed by Cre-mediated recombination in ES cells prior to the production of chimeric mice. [MGI Ref ID J:67797]

Genotyping

Genotyping Information

Genotyping Protocols

Ctnna1^tm1Efu, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Vasioukhin V; Bauer C; Degenstein L; Wise B; Fuchs E. 2001. Hyperproliferation and defects in epithelial polarity upon conditional ablation of alpha-catenin in skin. Cell 104(4):605-17. [PubMed: 11239416]  [MGI Ref ID J:67797]

Additional References

Ctnna1^tm1Efu related

Beronja S; Livshits G; Williams S; Fuchs E. 2010. Rapid functional dissection of genetic networks via tissue-specific transduction and RNAi in mouse embryos. Nat Med :. [PubMed: 20526348]  [MGI Ref ID J:160810]

Chen S; Lewis B; Moran A; Xie T. 2012. Cadherin-mediated cell adhesion is critical for the closing of the mouse optic fissure. PLoS One 7(12):e51705. [PubMed: 23240058]  [MGI Ref ID J:195672]

Joeng KS; Schumacher CA; Zylstra-Diegel CR; Long F; Williams BO. 2011. Lrp5 and Lrp6 redundantly control skeletal development in the mouse embryo. Dev Biol 359(2):222-9. [PubMed: 21924256]  [MGI Ref ID J:178499]

Kobielak A; Fuchs E. 2006. Links between alpha-catenin, NF-kappaB, and squamous cell carcinoma in skin. Proc Natl Acad Sci U S A 103(7):2322-7. [PubMed: 16452166]  [MGI Ref ID J:106064]

Lien WH; Klezovitch O; Fernandez TE; Delrow J; Vasioukhin V. 2006. alphaE-catenin controls cerebral cortical size by regulating the hedgehog signaling pathway. Science 311(5767):1609-12. [PubMed: 16543460]  [MGI Ref ID J:106707]

Lien WH; Klezovitch O; Null M; Vasioukhin V. 2008. alphaE-catenin is not a significant regulator of beta-catenin signaling in the developing mammalian brain. J Cell Sci 121(Pt 9):1357-62. [PubMed: 18397997]  [MGI Ref ID J:139820]

Livshits G; Kobielak A; Fuchs E. 2012. Governing epidermal homeostasis by coupling cell-cell adhesion to integrin and growth factor signaling, proliferation, and apoptosis. Proc Natl Acad Sci U S A 109(13):4886-91. [PubMed: 22411810]  [MGI Ref ID J:182219]

Nemade RV; Bierie B; Nozawa M; Bry C; Smith GH; Vasioukhin V; Fuchs E; Hennighausen L. 2004. Biogenesis and function of mouse mammary epithelium depends on the presence of functional alpha-catenin. Mech Dev 121(1):91-9. [PubMed: 14706703]  [MGI Ref ID J:87469]

Sheikh F; Chen Y; Liang X; Hirschy A; Stenbit AE; Gu Y; Dalton ND; Yajima T; Lu Y; Knowlton KU; Peterson KL; Perriard JC; Chen J. 2006. alpha-E-catenin inactivation disrupts the cardiomyocyte adherens junction, resulting in cardiomyopathy and susceptibility to wall rupture. Circulation 114(10):1046-55. [PubMed: 16923756]  [MGI Ref ID J:124719]

Silvis MR; Kreger BT; Lien WH; Klezovitch O; Rudakova GM; Camargo FD; Lantz DM; Seykora JT; Vasioukhin V. 2011. alpha-catenin is a tumor suppressor that controls cell accumulation by regulating the localization and activity of the transcriptional coactivator Yap1. Sci Signal 4(174):ra33. [PubMed: 21610251]  [MGI Ref ID J:185995]

Stocker AM; Chenn A. 2009. Focal reduction of alphaE-catenin causes premature differentiation and reduction of beta-catenin signaling during cortical development. Dev Biol 328(1):66-77. [PubMed: 19389371]  [MGI Ref ID J:149461]

Tinkle CL; Pasolli HA; Stokes N; Fuchs E. 2008. New insights into cadherin function in epidermal sheet formation and maintenance of tissue integrity. Proc Natl Acad Sci U S A 105(40):15405-10. [PubMed: 18809908]  [MGI Ref ID J:144091]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.