Strain Name:

B6;129-Ctnna1tm1Efu/J

Stock Number:

004604

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6;129-Catna1tm1Efu/J    (Changed: 02-JUN-05 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Elaine Fuchs,   The Rockefeller University

Description
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain expressing Cre recombinase in the female germline (see Stock No. 003551 for example), this mutant mouse strain may be useful in studies of mammary epithelium.

When bred to a strain expressing Cre recombinase in the mammary gland (see Stock No. 003552 for example), this mutant mouse strain may be useful in studies of mammary epithelium.

When bred to a strain expressing Cre recombinase in the nervous system (see Stock No. 003771 for example), this mutant mouse strain may be useful in studies of the cerebral cortex and the hedgehog signalling pathway.

When bred to a strain expressing tamoxifen-inducible Cre recombinase in juvenile and adult cardiac myocytes (see Stock No. 005657 for example), this mutant mouse strain may be useful in studies of dilated cardiomyopathy.

Development
A loxP site flanked targeting vector containing a neomycin resistance gene was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 2 of the targeted gene, and another loxP site was inserted upstream of exon 2. This construct was electroporated into 129/Sv derived GS1 embryonic stem (ES) cells which were transiently transfected with a vector containing the Cre-recombinase gene under the control of the cytomegalovirus promoter to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6J blastocysts.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Ctnna1tm1Efu/Ctnna1tm1Efu

        involves: 129X1/SvJ
  • integument phenotype
  • abnormal skin physiology
    • mice exhibit increased apoptosis in the epidermis compared to wild-type mice   (MGI Ref ID J:144091)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Ctnna1tm1Efu/Ctnna1tm1Efu Tg(MMTV-cre)4Mam/?

        involves: 129X1/SvJ * FVB   (conditional)
  • endocrine/exocrine gland phenotype
  • abnormal mammary gland epithelium morphology
    • mammary glands have reduced numbers of epithelial cells   (MGI Ref ID J:87469)
    • some epithelial cells remain condensed and disorganized without forming alveolar structures   (MGI Ref ID J:87469)
    • lumens are absent from clusters of epithelium   (MGI Ref ID J:87469)
  • abnormal mammary gland epithelium physiology
    • apoptosis in mammary epithelium is increased   (MGI Ref ID J:87469)
  • abnormal milk composition
    • milk proteins are reduced   (MGI Ref ID J:87469)
  • integument phenotype
  • abnormal hair follicle development
    • occasionally hair development is not on schedule   (MGI Ref ID J:87469)
  • abnormal mammary gland epithelium morphology
    • mammary glands have reduced numbers of epithelial cells   (MGI Ref ID J:87469)
    • some epithelial cells remain condensed and disorganized without forming alveolar structures   (MGI Ref ID J:87469)
    • lumens are absent from clusters of epithelium   (MGI Ref ID J:87469)
  • abnormal mammary gland epithelium physiology
    • apoptosis in mammary epithelium is increased   (MGI Ref ID J:87469)
  • abnormal milk composition
    • milk proteins are reduced   (MGI Ref ID J:87469)

Ctnna1tm1Efu/Ctnna1tm1Efu Tg(Myh6-cre/Esr1*)1Jmk/0

        involves: 129X1/SvJ * FVB/N   (conditional)
  • cardiovascular system phenotype
  • dilated cardiomyopathy
    • progressive 8 months after tamoxifen treatment   (MGI Ref ID J:197814)
  • muscle phenotype
  • dilated cardiomyopathy
    • progressive 8 months after tamoxifen treatment   (MGI Ref ID J:197814)

Ctnna1tm1Efu/Ctnna1tm1Efu Tg(Wap-cre)11738Mam/?

        involves: 129X1/SvJ * C57BL/6 * SJL   (conditional)
  • growth/size/body phenotype
  • decreased body weight
    • pups with homozygous mothers weight 50% of normal mice   (MGI Ref ID J:87469)
  • postnatal growth retardation
    • pups with homozygous mothers fail to thrive   (MGI Ref ID J:87469)
  • endocrine/exocrine gland phenotype
  • abnormal mammary gland epithelium morphology
    • mammary glands have reduced numbers of epithelial cells   (MGI Ref ID J:87469)
    • some epithelial cells remain condensed and disorganized without forming alveolar structures   (MGI Ref ID J:87469)
    • lumens are absent from clusters of epithelium   (MGI Ref ID J:87469)
    • apoptosis in mammary epithelium is increased   (MGI Ref ID J:87469)
  • abnormal milk composition
    • milk proteins are reduced   (MGI Ref ID J:87469)
  • other phenotype
  • maternal effect
    • pups with homozygous mothers fail to thrive, are about 50% of normal weight and some do not survive lactation   (MGI Ref ID J:87469)
    • in litters that experience lethality, the litters are reduced to 50% at day 5 and 25% by day 10   (MGI Ref ID J:87469)
  • integument phenotype
  • abnormal mammary gland epithelium morphology
    • mammary glands have reduced numbers of epithelial cells   (MGI Ref ID J:87469)
    • some epithelial cells remain condensed and disorganized without forming alveolar structures   (MGI Ref ID J:87469)
    • lumens are absent from clusters of epithelium   (MGI Ref ID J:87469)
    • apoptosis in mammary epithelium is increased   (MGI Ref ID J:87469)
  • abnormal milk composition
    • milk proteins are reduced   (MGI Ref ID J:87469)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Developmental Biology Research
      Cre-lox System
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Cre-lox System

Ctnna1tm1Efu related

Cell Biology Research
Defects in Cell Adhesion Molecules

Developmental Biology Research
Defects in Cell Adhesion Molecules

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ctnna1tm1Efu
Allele Name targeted mutation 1, Elaine Fuchs
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) alpha-cat fl; floxed alpha-E-catenin;
Strain of Origin129X1/SvJ
ES Cell Line NameRW-4
ES Cell Line Strain129X1/SvJ
Gene Symbol and Name Ctnna1, catenin (cadherin associated protein), alpha 1
Chromosome 18
Gene Common Name(s) 2010010M04Rik; AA517462; AI988031; CAP102; Catna1; RIKEN cDNA 2010010M04 gene; alpha E catenin; alpha(E)-catenin; catenin alpha 1; expressed sequence AA517462; expressed sequence AI988031;
Molecular Note LoxP sites were inserted by homologous recombination that flanked exon 2. An adjacent floxed neomycin selection cassette was removed by Cre-mediated recombination in ES cells prior to the production of chimeric mice. [MGI Ref ID J:67797]

Genotyping

Genotyping Information

Genotyping Protocols

Ctnna1tm1Efu, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Vasioukhin V; Bauer C; Degenstein L; Wise B; Fuchs E. 2001. Hyperproliferation and defects in epithelial polarity upon conditional ablation of alpha-catenin in skin. Cell 104(4):605-17. [PubMed: 11239416]  [MGI Ref ID J:67797]

Additional References

Ctnna1tm1Efu related

Beronja S; Livshits G; Williams S; Fuchs E. 2010. Rapid functional dissection of genetic networks via tissue-specific transduction and RNAi in mouse embryos. Nat Med :. [PubMed: 20526348]  [MGI Ref ID J:160810]

Chen S; Lewis B; Moran A; Xie T. 2012. Cadherin-mediated cell adhesion is critical for the closing of the mouse optic fissure. PLoS One 7(12):e51705. [PubMed: 23240058]  [MGI Ref ID J:195672]

Joeng KS; Schumacher CA; Zylstra-Diegel CR; Long F; Williams BO. 2011. Lrp5 and Lrp6 redundantly control skeletal development in the mouse embryo. Dev Biol 359(2):222-9. [PubMed: 21924256]  [MGI Ref ID J:178499]

Kobielak A; Fuchs E. 2006. Links between alpha-catenin, NF-kappaB, and squamous cell carcinoma in skin. Proc Natl Acad Sci U S A 103(7):2322-7. [PubMed: 16452166]  [MGI Ref ID J:106064]

Li J; Goossens S; van Hengel J; Gao E; Cheng L; Tyberghein K; Shang X; De Rycke R; van Roy F; Radice GL. 2012. Loss of alphaT-catenin alters the hybrid adhering junctions in the heart and leads to dilated cardiomyopathy and ventricular arrhythmia following acute ischemia. J Cell Sci 125(Pt 4):1058-67. [PubMed: 22421363]  [MGI Ref ID J:197814]

Lien WH; Klezovitch O; Fernandez TE; Delrow J; Vasioukhin V. 2006. alphaE-catenin controls cerebral cortical size by regulating the hedgehog signaling pathway. Science 311(5767):1609-12. [PubMed: 16543460]  [MGI Ref ID J:106707]

Lien WH; Klezovitch O; Null M; Vasioukhin V. 2008. alphaE-catenin is not a significant regulator of beta-catenin signaling in the developing mammalian brain. J Cell Sci 121(Pt 9):1357-62. [PubMed: 18397997]  [MGI Ref ID J:139820]

Livshits G; Kobielak A; Fuchs E. 2012. Governing epidermal homeostasis by coupling cell-cell adhesion to integrin and growth factor signaling, proliferation, and apoptosis. Proc Natl Acad Sci U S A 109(13):4886-91. [PubMed: 22411810]  [MGI Ref ID J:182219]

Nemade RV; Bierie B; Nozawa M; Bry C; Smith GH; Vasioukhin V; Fuchs E; Hennighausen L. 2004. Biogenesis and function of mouse mammary epithelium depends on the presence of functional alpha-catenin. Mech Dev 121(1):91-9. [PubMed: 14706703]  [MGI Ref ID J:87469]

Sheikh F; Chen Y; Liang X; Hirschy A; Stenbit AE; Gu Y; Dalton ND; Yajima T; Lu Y; Knowlton KU; Peterson KL; Perriard JC; Chen J. 2006. alpha-E-catenin inactivation disrupts the cardiomyocyte adherens junction, resulting in cardiomyopathy and susceptibility to wall rupture. Circulation 114(10):1046-55. [PubMed: 16923756]  [MGI Ref ID J:124719]

Silvis MR; Kreger BT; Lien WH; Klezovitch O; Rudakova GM; Camargo FD; Lantz DM; Seykora JT; Vasioukhin V. 2011. alpha-catenin is a tumor suppressor that controls cell accumulation by regulating the localization and activity of the transcriptional coactivator Yap1. Sci Signal 4(174):ra33. [PubMed: 21610251]  [MGI Ref ID J:185995]

Stocker AM; Chenn A. 2009. Focal reduction of alphaE-catenin causes premature differentiation and reduction of beta-catenin signaling during cortical development. Dev Biol 328(1):66-77. [PubMed: 19389371]  [MGI Ref ID J:149461]

Takezawa Y; Yoshida K; Miyado K; Sato M; Nakamura A; Kawano N; Sakakibara K; Kondo T; Harada Y; Ohnami N; Kanai S; Miyado M; Saito H; Takahashi Y; Akutsu H; Umezawa A. 2011. beta-catenin is a molecular switch that regulates transition of cell-cell adhesion to fusion. Sci Rep 1:68. [PubMed: 22355587]  [MGI Ref ID J:206123]

Tinkle CL; Pasolli HA; Stokes N; Fuchs E. 2008. New insights into cadherin function in epidermal sheet formation and maintenance of tissue integrity. Proc Natl Acad Sci U S A 105(40):15405-10. [PubMed: 18809908]  [MGI Ref ID J:144091]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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