Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Generation N1F?+F11 (22-MAY-08)   Donating Investigator Elaine Fuchs,   The Rockefeller University

Description
These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

When bred to a strain expressing Cre recombinase in the epithelial cells of the intestine (see Stock No. 004586 for example), this mutant mouse strain may be useful in studies of intestinal hyperplasia.

Development
A loxP site flanked targeting vector containing neomycin resistance and phosphoglycerate kinase genes was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 3 of the targeted gene, and another loxP site was inserted upstream of exon 3. The construct was electroporated into 129X1/SvJ derived RW-4 embryonic stem (ES) cells which were transiently transfected with a vector containing the Cre-recombinase gene under the control of the cytomegalovirus promoter to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6J blastocysts.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Itgb1

003096

STOCK Itgb1tm1Lscd/J

View Strains carrying other alleles of Itgb1     (1 strain)

Additional Web Information

Cre-lox Systems

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX^® Services jaxservices@jax.org for customized breeding options.

Itgb1^tm1Efu/Itgb1^tm1Efu Tg(Vil-cre)997Gum/0

        involves: 129X1/SvJ * C57BL/6J * SJL   (conditional)

• lethality-postnatal
• postnatal lethality (MGI Ref ID J:119155)
  • die between P7 and P14 from severe malnutrition
• growth/size phenotype
• decreased body weight (MGI Ref ID J:119155)
  • by P4, mutants are less than half the body weight of controls
• digestive/alimentary phenotype
• abnormal intestine morphology (MGI Ref ID J:119155)
  • expansion of the intestinal stroma, muscularis, and extracellular matrix
• abnormal intestinal epithelium morphology (MGI Ref ID J:119155)
  • mutants exhibit an increase in intestinal epithelial cell proliferation in the crypts with dysplasia and polyps, resulting in an expanded epithelium
  • the small intestinal epithelium shows a defective microvillus brush border on the apical surfaces of the villous enterocytes
• abnormal crypts of Lieberkuhn morphology (MGI Ref ID J:119155)
  • intestinal crypt and villi expansion, enlargement, and dysplasia at P16
  • crypt hyperplasia is most severe in the distal small intestine, proximal large intestine, and cecum
• abnormal enterocyte morphology (MGI Ref ID J:119155)
  • the intestinal epithelium shows large lipid inclusions within the villous enterocytes that are not seen in controls
  • the intestinal microvilli are diminished in size and poorly formed, indicatiang defective enterocyte differentiation
• abnormal large intestine morphology (MGI Ref ID J:119155)
  • proximal large intestine is larger in external diameter than in controls
• abnormal small intestine morphology (MGI Ref ID J:119155)
  • distal small intestine is larger in external diameter than in controls
• intestinal polyps (MGI Ref ID J:119155)
  • multiple juvenile-like polyps in the small intestinal mucosa
• abnormal nutrient absorption (MGI Ref ID J:119155)
  • although mutants can feed, they are malnourished due to intestinal epithelium defects
• steatorrhea (MGI Ref ID J:119155)
  • fat malabsorption as indicated by the presence of large fat droplets in the intestinal contents
• homeostasis/metabolism phenotype
• abnormal circulating lipid level (MGI Ref ID J:119155)
  • total serum lipid levels are reduced
• endocrine/exocrine gland phenotype
• abnormal crypts of Lieberkuhn morphology (MGI Ref ID J:119155)
  • intestinal crypt and villi expansion, enlargement, and dysplasia at P16
  • crypt hyperplasia is most severe in the distal small intestine, proximal large intestine, and cecum

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System (loxP-flanked Sequences)

Itgb1^tm1Efu related

Developmental Biology Research
Defects in Cell Adhesion Molecules
Embryonic Lethality (Homozygous)
Postnatal Mortality

Genes & Alleles

Gene & Allele Information

Allele Symbol		Itgb1tm1Efu
Allele Name		targeted mutation 1, Elaine Fuchs
Allele Type		Targeted (Floxed/Frt)
Common Name(s)		CD29f; Itgb1flox; beta1flox; integrin beta1;
Mutation Made By		Elaine Fuchs,   The Rockefeller University
Strain of Origin		129X1/SvJ
ES Cell Line Name		RW-4
ES Cell Line Strain		129X1/SvJ
Gene Symbol and Name		Itgb1, integrin beta 1 (fibronectin receptor beta)
Chromosome		8
Gene Common Name(s)		4633401G24Rik; AA409975; AA960159; CD29; FNRB; Fnrb; GPIIA; MDF2; MSK12; RIKEN cDNA 4633401G24 gene; VLA-BETA; VLAB; beta1 integrin; expressed sequence AA409975; expressed sequence AA960159; fibronectin receptor beta (integrin);
Molecular Note		Insertion of loxP sites flanking the third exon of the Itgb1 gene. No effect on the function of the Itgb1 gene. [MGI Ref ID J:65039]

Genotyping

Genotyping Information

Genotyping Protocols

Itgb1^tm1Efu, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Raghavan S; Bauer C; Mundschau G; Li Q; Fuchs E. 2000. Conditional ablation of beta1 integrin in skin. Severe defects in epidermal proliferation, basement membrane formation, and hair follicle invagination. J Cell Biol 150(5):1149-60. [PubMed: 10974002]  [MGI Ref ID J:65039]

Additional References

Itgb1^tm1Efu related

Chan CS; Weeber EJ; Zong L; Fuchs E; Sweatt JD; Davis RL. 2006. Beta1-integrins are required for hippocampal AMPA receptor-dependent synaptic transmission, synaptic plasticity, and working memory. J Neurosci 26(1):223-32. [PubMed: 16399691]  [MGI Ref ID J:104150]

Chen H; Zou Z; Sarratt KL; Zhou D; Zhang M; Sebzda E; Hammer DA; Kahn ML. 2006. In vivo beta1 integrin function requires phosphorylation-independent regulation by cytoplasmic tyrosines. Genes Dev 20(8):927-32. [PubMed: 16618804]  [MGI Ref ID J:108331]

Jones RG; Li X; Gray PD; Kuang J; Clayton F; Samowitz WS; Madison BB; Gumucio DL; Kuwada SK. 2006. Conditional deletion of beta1 integrins in the intestinal epithelium causes a loss of Hedgehog expression, intestinal hyperplasia, and early postnatal lethality. J Cell Biol 175(3):505-14. [PubMed: 17088430]  [MGI Ref ID J:119155]

Kanasaki K; Kanda Y; Palmsten K; Tanjore H; Lee SB; Lebleu VS; Gattone VH Jr; Kalluri R. 2008. Integrin beta1-mediated matrix assembly and signaling are critical for the normal development and function of the kidney glomerulus. Dev Biol 313(2):584-93. [PubMed: 18082680]  [MGI Ref ID J:130822]

Li H; Oliver T; Jia W; He YW. 2006. Efficient dendritic cell priming of T lymphocytes depends on the extracellular matrix protein mindin. EMBO J 25(17):4097-107. [PubMed: 16917498]  [MGI Ref ID J:112875]

Pozzi A; Jarad G; Moeckel GW; Coffa S; Zhang X; Gewin L; Eremina V; Hudson BG; Borza DB; Harris RC; Holzman LB; Phillips CL; Fassler R; Quaggin SE; Miner JH; Zent R. 2008. Beta1 integrin expression by podocytes is required to maintain glomerular structural integrity. Dev Biol 316(2):288-301. [PubMed: 18328474]  [MGI Ref ID J:135414]

Raghavan S; Vaezi A; Fuchs E. 2003. A role for alphabeta1 integrins in focal adhesion function and polarized cytoskeletal dynamics. Dev Cell 5(3):415-27. [PubMed: 12967561]  [MGI Ref ID J:109020]

Simirskii VN; Wang Y; Duncan MK. 2007. Conditional deletion of beta1-integrin from the developing lens leads to loss of the lens epithelial phenotype. Dev Biol 306(2):658-68. [PubMed: 17493607]  [MGI Ref ID J:122566]

Tanjore H; Zeisberg EM; Gerami-Naini B; Kalluri R. 2008. Beta1 integrin expression on endothelial cells is required for angiogenesis but not for vasculogenesis. Dev Dyn 237(1):75-82. [PubMed: 18058911]  [MGI Ref ID J:130422]

Wang QQ; Li H; Oliver T; Glogauer M; Guo J; He YW. 2008. Integrin beta1 regulates phagosome maturation in macrophages through Rac expression. J Immunol 180(4):2419-28. [PubMed: 18250451]  [MGI Ref ID J:131992]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	Resulting chimeric animals were bred to C57BL/6J mice once before being made homozygous. Expected coat colors are: dark brown (almost black) it is suspected that they are a/a Tyr<c-ch>/Tyr<c-ch>.
Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

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“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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