Strain Name: |
STOCK Tg(Fos-lacZ)34Efu/J |
|---|---|
Stock Number: |
004623 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Fos; Gnat2; Gnat2cpfl3; lacZ; Tg(Fos-lacZ)34Efu; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Elaine Fuchs, The Rockefeller University Generation N1F10 (05-DEC-07) Important Note
This strain is homozygous for Gnat2cpfl3, cone photoreceptor function loss 3, which affects bright light (photopic) vision.Strain Description
These TOPGAL transgenic mice are a reporter strain that express Beta-galactosidase in the presence of the lymphoid enhancer binding factor 1/transcription factor 3 (LEF/TCF) mediated signaling pathway and activated Beta-catenin. The transgene contains the lacZ gene under the control of a regulatory sequence consisting of three consensus LEF/TCF-binding motifs upstream of a minimal c-fos promoter. Transgenic mice display TOPGAL activity (Beta-galactosidase activity) during early embryonic development in a subset of pluripotent embryonic basal cells of the epithelium and dermis of developing hair follicles, but not during the next stage of hair follicle development; formation of hair germs. TOPGAL transgene activity reappears in hair follicles at E16.5 and TOPGAL expression is strongly upregulated in the postnatal hair shaft precursor cells in both whisker and body hair anagen follicles (active periods of hair growth). TOPGAL expression ceases during catagen (regression and shortening) and telogen (rest) periods of the postnatal hair growth cycle. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain represents an effective tool for generating mutants that would be useful in studies of the Wnt signaling pathway.Strain Development
A transgenic construct containing the lacZ gene under the control of a promoter consisting of three consensus LEF-/TCF-binding motifs upstream of a minimal c-fos promoter was used to create transgenic animals on a CD1 background.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Fos-lacZ)34Efu | ||
|---|---|---|---|
| Allele Name | transgene insertion 34, Elaine Fuchs | ||
| Common Name(s) | TCF-betagal; TOPGAL; Top-Gal; | ||
| Mutation Made By | Elaine Fuchs, The Rockefeller University | ||
| Site of Expression | lacZ expression occurs during early embryonic development in a subset of pluripotent embryonic basel cells of the epithelium and dermis of developing hair follicles. lacZ expression disappears during formation of hair germ and then reappears at E16.5 in hair follicles until 18 days after birth. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | Fos, FBJ osteosarcoma oncogene, rat | ||
| General Note |
Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Transgenic mice express beta-galactosidase in the presence of the lymphoid enhancer binding factor 1/transcription factor 3 (LEF1/TCF3) mediated signaling pathway and activated Beta-catenin (CATNB). Transgenic mice display Beta-galactosidase activity during early embryonic development in a subset of pluripotent embryonic basal cells of the epithelium and dermis of developing hair follicles. Beta-galactosidase activity is not detected in the next stage of hair follicle development, formation of hair germs. At E16.5, transgene activity reappears in hair follicles and is detectable until 18 days after birth. | ||
| Molecular Note | The transgene contains the lacZ gene under the control of a promoter consisting of three consensus LEF-/TCF-binding motifs upstream of a minimal Fos promoter. [MGI Ref ID J:55937] | ||
| Allele Symbol | Gnat2cpfl3 | ||
| Allele Name | cone photoreceptor function loss 3 | ||
| Common Name(s) | Gnat2; | ||
| Strain of Origin | various | ||
| Gene Symbol and Name | Gnat2, guanine nucleotide binding protein, alpha transducing 2 | ||
| Chromosome | 3 | ||
| Gene Common Name(s) | ACHM4; AW490837; GNATC; Gnat-2; Gt-2; Tcalpha; expressed sequence AW490837; | ||
| General Note |
This allele has been detected in the following strains either by genotyping or complementation testing: ALS/LtJ, SENCARA/PtJ, SENCARB/PtJ, SENCARC/PtJ, PN/nBSwUmabJ. (J:122428) Phenotypic Similarity to Human Syndrome in Orthologous Human Gene: OMIM +139340 ACHROMATOPSIA 4. | ||
| Molecular Note | A single nucleotide substitution of G to A at position 598 in exon 6. This mutation converts codon 200 from apartic acid to asparagine. [MGI Ref ID J:122428] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Colony Maintenance
| Breeding & Husbandry | This strain is maintained as a hemizygote on a stock CD1 background. Coat color expected from breeding:Albino |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (174 strains)
002448 129S1/SvImJ 003072 ALS/LtJ 006180 CD10/JlsJ 005052 PN/nBSwUmabJ 002746 SENCARA/PtJ 002747 SENCARB/PtJ 002748 SENCARC/PtJ 006135 STOCK Sgk3fz-ica/McirJ 003773 STOCK Tg(CAG-ECFP)CK6Nagy/J 005645 STOCK Tg(CAG-mRFP1)1F1Hadj/J 005667 STOCK Tg(Neurog3-cre)C1Able/J 003262 STOCK Tg(Trp53A135V)L3Ber/J 005104 STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J 005699 STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
003479 B6.C3-Tg(Fos-luc)1Rnd/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
lacZ relatedCell Biology Research
Signal Transduction
Dermatology Research
Other
Developmental Biology Research
Skin and Hair Texture Defects
Research Tools
lacZ Expression
Dermatology Research
Developmental Biology Research
Genetics Research (Tissue/Cell Markers)
Research Tools
lacZ Expression
References
Selected Reference(s)
Additional ReferencesDasGupta R; Fuchs E. 1999. Multiple roles for activated LEF/TCF transcription complexes during hair follicle development and differentiation. Development 126(20):4557-68. [PubMed: 10498690] [MGI Ref ID J:55937]
Price and Supply Information
| Strain Name: | STOCK Tg(Fos-lacZ)34Efu/J |
| Stock Number: | 004623 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $301.60 | Hemizygous for Tg(Fos-lacZ)34Efu | |||
| Pair | $369.60 | Hemizygous for Tg(Fos-lacZ)34Efu x Noncarrier | |||
| Pair | $369.60 | Noncarrier x Hemizygous for Tg(Fos-lacZ)34Efu | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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