Description

Strain Information

Former Names wdl/wdl    (Changed: 03-FEB-06 ) C57BLKS/J-wdl/J    (Changed: 03-FEB-06 ) Type Coisogenic; Mutant Strain; Spontaneous Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse H2 Haplotype d Generation F39 (07-DEC-07)

Appearance
black, waddles
Related Genotype: a/a Car8^wdl/Car8^wdl

black, unaffected
Related Genotype: a/a Car8^wdl/+

Description
wdl homozygotes display a side-to-side wobble in their gait as early as two weeks of age and this persists throughout life. Despite this, they can swim in a straight line.

Development
The waddles mutation arose spontaneously in the C57BLKS/J production colony at The Jackson Laboratory in 1995 when that strain was at F104. The waddles bearing strain has been maintained by sibling mating and in 2004 reached F24.

Control Information

	Control
	Heterozygote from the colony
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Car8^wdl/Car8^wdl

        C57BLKS/J

• behavior/neurological phenotype
• abnormal gait (MGI Ref ID J:81344)
  • this mutant has an odd walk and waddles

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Ataxia (Movement) Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Car8wdl
Allele Name		waddles
Allele Type		Spontaneous
Common Name(s)		rig; rigoletto;
Strain of Origin		C57BLKS/J
Gene Symbol and Name		Car8, carbonic anhydrase 8
Chromosome		4
Gene Common Name(s)		AW546993; CA-RP VIII; CA-VIII; CALS; CARP; Ca8; Cals1; Carp; MGC105764; MGC120502; MGC99509; carbonic anhydrase related polypeptide; carbonic anhydrase-like sequence 1; expressed sequence AW546993; waddles; wdl;
General Note		This mutation arose at The Jackson Laboratory. Homozygous mutant mice display a wobbly side-to-side gait which is noticable by two weeks of age and remains phenotypically similar throughout life. Some, but not all, of the homozygous mutant mice are slightly smaller than normal littermates. Homozygotes do not clasp their hind legs when picked up by the tail. Both mutant and control mice swim in a straight line when placed in water. Heterozygotes are indistinguishable from their normal littermates. Males and females are viable and fertile.
Molecular Note		Mutant mice contain a 19-nucleotide deletion, 15 of which are flanked by two AAGG motifs. Car8 transcript is absent in homozygous mice, suggesting that this is a null mutation. [MGI Ref ID J:103673]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Harris BS; Ward-Bailey PF; Johnson KR; Bronson R; Davisson MT. 2003. A new mutation, waddles (wdl), on mouse Chromosome 4. Mouse Mutant Resources Web Site, The Jackson Laboratory, Bar Harbor, Maine (http://www.jax.org/mmr) MGI Direct Data Submission :.  [MGI Ref ID J:81344]

Jiao Y; Yan J; Zhao Y; Donahue LR; Beamer WG; Li X; Roe BA; Ledoux MS; Gu W. 2005. Carbonic anhydrase-related protein VIII deficiency is associated with a distinctive lifelong gait disorder in waddles mice. Genetics 171(3):1239-46. [PubMed: 16118194]  [MGI Ref ID J:103673]

Additional References

Car8^wdl related

Hirasawa M; Xu X; Trask RB; Maddatu TP; Johnson BA; Naggert JK; Nishina PM; Ikeda A. 2007. Carbonic anhydrase related protein 8 mutation results in aberrant synaptic morphology and excitatory synaptic function in the cerebellum. Mol Cell Neurosci 35(1):161-70. [PubMed: 17376701]  [MGI Ref ID J:123229]

Yan J; Jiao Y; Jiao F; Stuart J; Donahue LR; Beamer WG; Li X; Roe BA; LeDoux MS; Gu W. 2007. Effects of carbonic anhydrase VIII deficiency on cerebellar gene expression profiles in the wdl mouse. Neurosci Lett 413(3):196-201. [PubMed: 17174474]  [MGI Ref ID J:119094]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           A1

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Mouse Mutant Resource collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Heterozygote from the colony
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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