Strain Name:

C3H/HeDiSnJ-Vamp1lew/GrsrJ

Stock Number:

004626

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Availability:

Cryopreserved - Ready for recovery

Mice homozygous for this recessive mutation are recognized by their wasting pre-weaning lethal phenotype, they curl up, waste away and die by 21 days. Mutants do not live to breed but no lesions have been found pathologically.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C3H/HeDiSnJ-Vamp1lew/J    (Changed: 22-JUN-09 )
Type Coisogenic; Mutant Strain; Spontaneous Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
H2 Haplotypek

Appearance
agouti, unaffected
Related Genotype: A/A Vamp1lew/+

Description
Mice homozygous for this recessive mutation are recognized by their wasting pre-weaning lethal phenotype, they curl up, waste away and die by 21 days. VAMP1 mRNA was found to be significantly downregulated in the lethal-wasting brain compared to wild-type littermates. Mutants do not live to breed but no lesions have been found pathologically.

Development
The lew mutation arose spontaneously in 1985 in a mutation-bearing C3H/HeDiSnJ subline at The Jackson Laboratory and has been maintained on the same background.

Control Information

  Control
   +/? untyped from the colony
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Spastic Ataxia 1, Autosomal Dominant; SPAX1   (VAMP1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Vamp1lew/Vamp1lew

        involves: C3H/HeDiSnJ
  • mortality/aging
  • complete postnatal lethality
    • mutants die by 3 weeks of age   (MGI Ref ID J:81354)
  • behavior/neurological phenotype
  • abnormal posture
    • mutants lie on their sides with their front paws curled inward and their back legs held tightly together   (MGI Ref ID J:81354)
  • growth/size/body phenotype
  • decreased body size
    • mutants can be recognized at 2 weeks by their small size (about 30% smaller than littermates)   (MGI Ref ID J:81354)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Vamp1lew/Vamp1lew

        involves: C3H/HeDiSnJ * CAST/EiJ
  • mortality/aging
  • postnatal lethality
    • affected mice die at around 15 days of age   (MGI Ref ID J:148602)
  • behavior/neurological phenotype
  • abnormal posture
    • 10 day old mice lay on their side   (MGI Ref ID J:148602)
  • akinesia
    • noticably immobile at 10 days of age   (MGI Ref ID J:148602)
  • impaired limb coordination
    • limb movement occurs in 10 day old mice but is not effective   (MGI Ref ID J:148602)
  • impaired righting response
    • 10 day old mice do not attempt to right themselves when place on their backs   (MGI Ref ID J:148602)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Vamp1lew related

Developmental Biology Research
Growth Defects
Postnatal Lethality
      Homozygous

Metabolism Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Vamp1lew
Allele Name lethal wasting
Allele Type Spontaneous
Common Name(s) Syb1lew;
Strain of OriginC3H/HeDiSnJ
Gene Symbol and Name Vamp1, vesicle-associated membrane protein 1
Chromosome 6
Gene Common Name(s) SPAX1; SYB1; Syb-1; Syb1; VAMP-1; lethal wasting; lew; synaptobrevin 1;
Molecular Note A spontaneous mutant identified at The Jackson Laboratory. A G190T transversion creates a premature stop codon. The predicted protein that results would lack both a transmembrane domain and a calmodulin interaction domain. mRNA levels are severely reduced and no protein can be detected by immunohistochemistry in the brain. [MGI Ref ID J:148602]

Genotyping

Genotyping Information

Genotyping Protocols

Vamp1lew, Pyrosequencing


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Harris BS; Ward-Bailey P; Johnson KR; Bronson R; Davisson MT. 2003. Lethal Wasting (lew), a New Prewean Lethal Mouse Mutation on Chromosome 6, 2003, Mouse Mutant Resource Web Site, The Jackson Laboratory, Bar Harbor, Maine. World Wide Web MGI Direct Data Submission :.  [MGI Ref ID J:81354]

Additional References

Vamp1lew related

Nouvian R; Neef J; Bulankina AV; Reisinger E; Pangrsic T; Frank T; Sikorra S; Brose N; Binz T; Moser T. 2011. Exocytosis at the hair cell ribbon synapse apparently operates without neuronal SNARE proteins. Nat Neurosci 14(4):411-3. [PubMed: 21378973]  [MGI Ref ID J:172303]

Nystuen AM; Schwendinger JK; Sachs AJ; Yang AW; Haider NB. 2007. A null mutation in VAMP1/synaptobrevin is associated with neurological defects and prewean mortality in the lethal-wasting mouse mutant. Neurogenetics 8(1):1-10. [PubMed: 17102983]  [MGI Ref ID J:148602]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryBecause mice homozygous for this recessive mutation die by wean age, this strain needs to be progeny tested at each generation to identify heterozygous breeder pairs.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

  • View the complete collection of spontaneous mutants in the Mouse Mutant Resource.

Control Information

  Control
   +/? untyped from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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