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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6;CBA-Tg(Pou5f1-EGFP)2Mnn (Changed: 15-DEC-04 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation F?+F11 (29-SEP-08) Donating Investigator Jeff Mann, Beckman Research Institute, City of Hope Description
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein under the control of the POU domain, class 5, transcription factor 1, promoter and distal enhancer. Primordial germ cell specific markers, alkaline phosphatase II and stage-specific embryonic antigen, are co-expressed in EGFP positive cells. 9.5 and 10.5 dpc (days post-coitum) migratory primordial germ cells from hemizygotes and homozygotes can be sorted and isolated by flow cytometry. This strain represents an effective tool for studying genetic imprinting and early embryonic development.Development
A transgenic construct containing an Enhanced Green Fluorescent Protein gene under the control of the promoter and distal enhancer of the POU domain, class 5, transcription factor 1, Pou5fl, was injected into (CBA/CaJ X C57BL/6J)F2 fertilized eggs.
Control Information
| Control | ||
|---|---|---|
| 100011 B6CBAF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedDevelopmental Biology Research
Research Tools
Developmental Biology Research
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: primordial germ cell marker
Reproductive Biology Research
primordial germ cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Pou5f1-EGFP)2Mnn | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Jeff Mann | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | OG2; TgOG2; | ||
| Mutation Made By | Jeff Mann, Beckman Research Institute, City of Hope | ||
| Strain of Origin | (CBA/CaJ X C57BL/6J)F2 | ||
| Site of Expression | Primordial germ cell species markers, alkaline phosphastase II and stage-specific, embryonic antigen, are co-expressed in EGFP positive cells. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Pou5f1, POU domain, class 5, transcription factor 1, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Pou5f1-EGFP)2Mnn, transgene insertion 2, Jeff Mann | ||
| Chromosome | UN | ||
| Gene Common Name(s) | OG2; TgOG2; | ||
| Molecular Note | The transgene contains an Enhanced Green Fluorescent Protein gene under the control of the promoter and distal enhancer of the POU domain, class 5, transcription factor 1, (Pou5fl). [MGI Ref ID J:77318] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Szabo PE; Hubner K; Scholer H; Mann JR. 2002. Allele-specific expression of imprinted genes in mouse migratory primordial germ cells. Mech Dev 115(1-2):157-60. [PubMed: 12049782] [MGI Ref ID J:77318]
Additional References
Tg(Pou5f1-EGFP)2Mnn relatedCoussens M; Maresh JG; Yanagimachi R; Maeda G; Allsopp R. 2008. Sirt1 deficiency attenuates spermatogenesis and germ cell function. PLoS ONE 3(2):e1571. [PubMed: 18270565] [MGI Ref ID J:132188]
Shi Y; Desponts C; Do JT; Hahm HS; Scholer HR; Ding S. 2008. Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds. Cell Stem Cell 3(5):568-74. [PubMed: 18983970] [MGI Ref ID J:149867]
Zhao M; Rohozinski J; Sharma M; Ju J; Braun RE; Bishop CE; Meistrich ML. 2007. Utp14b: a unique retrogene within a gene that has acquired multiple promoters and a specific function in spermatogenesis. Dev Biol 304(2):848-59. [PubMed: 17291484] [MGI Ref ID J:122508]
van den Bergen JA; Miles DC; Sinclair AH; Western PS. 2009. Normalizing gene expression levels in mouse fetal germ cells. Biol Reprod 81(2):362-70. [PubMed: 19403927] [MGI Ref ID J:152931]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry Homozygous mice were generated and interbred. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $107.90 Female or Male Homozygous for Tg(Pou5f1-EGFP)2Mnn
Pairs /Price (US dollars $) Pair Genotype $215.80 Homozygous for Tg(Pou5f1-EGFP)2Mnn x Homozygous for Tg(Pou5f1-EGFP)2Mnn
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $140.30 Female or Male Homozygous for Tg(Pou5f1-EGFP)2Mnn
Pairs /Price (US dollars $) Pair Genotype $280.60 Homozygous for Tg(Pou5f1-EGFP)2Mnn x Homozygous for Tg(Pou5f1-EGFP)2Mnn
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 100011 B6CBAF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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