Strain Name: |
B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J |
|---|---|
Stock Number: |
004654 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6;CBA-Tg(Pou5f1-EGFP)2Mnn (Changed: 15-DEC-04
) |
| Genes & Alleles | GFP; Pou5f1; Tg(Pou5f1-EGFP)2Mnn; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Jeff Mann, Beckman Research Institute, City of Hope Generation F?+F10 (31-DEC-07) Strain Description
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein under the control of the POU domain, class 5, transcription factor 1, promoter and distal enhancer. Primordial germ cell specific markers, alkaline phosphatase II and stage-specific embryonic antigen, are co-expressed in EGFP positive cells. 9.5 and 10.5 dpc (days post-coitum) migratory primordial germ cells from hemizygotes and homozygotes can be sorted and isolated by flow cytometry. This strain represents an effective tool for studying genetic imprinting and early embryonic development.Strain Development
A transgenic construct containing an Enhanced Green Fluorescent Protein gene under the control of the promoter and distal enhancer of the POU domain, class 5, transcription factor 1, Pou5fl, was injected into (CBA/CaJ X C57BL/6J)F2 fertilized eggs.
Gene & Allele Details
| Allele Symbol | Tg(Pou5f1-EGFP)2Mnn | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Jeff Mann | ||
| Common Name(s) | OG2; TgOG2; | ||
| Mutation Made By | Jeff Mann, Beckman Research Institute, City of Hope | ||
| Strain of Origin | (CBA/CaJ X C57BL/6J)F2 | ||
| Site of Expression | Primordial germ cell species markers, alkaline phosphastase II and stage-specific, embryonic antigen, are co-expressed in EGFP positive cells. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Pou5f1, POU domain, class 5, transcription factor 1, mouse, laboratory | ||
| Molecular Note | The transgene contains an Enhanced Green Fluorescent Protein gene under the control of the promoter and distal enhancer of the POU domain, class 5, transcription factor 1, (Pou5fl). [J:77318] | ||
Control Information
| Control | ||
|---|---|---|
| 100011 B6CBAF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fluorescent Proteins
Colony Maintenance
| Breeding & Husbandry | Homozygous mice were generated and interbred. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
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Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedDevelopmental Biology Research
Research Tools
Developmental Biology Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: primordial germ cell marker)
Reproductive Biology Research (primordial germ cell marker)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesSzabo PE; Hubner K; Scholer H; Mann JR. 2002. Allele-specific expression of imprinted genes in mouse migratory primordial germ cells. Mech Dev 115(1-2):157-60. [PubMed: 12049782] [J:77318]
Price and Supply Information
| Strain Name: | B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J |
| Stock Number: | 004654 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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- Use of MICE by companies or for-profits entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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