Strain Name: |
B6.Cg-Tg(TIE2GFP)287Sato/1J |
|---|---|
Stock Number: |
004659 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | GFP; Tek; Tg(TIE2GFP)287Sato; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Inbred x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N10 (01-FEB-07) Strain Description
This strain expresses Green Fluorescent Protein (GFP) under the direction of the endothelial-specific receptor tyrosine kinase (Tek, formerly, Tie2) promoter. Endothelial cells expressing GFP can be visualized via fluorescent microscopy or purified by FACS.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
This strain originated on an FVB/N background, and was backcrossed to C57BL/6J for 5 generations using a speed congenic protocol. The C57BL/6J component of this speed congenic background is estimated at >95% via genome scanning.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(TIE2GFP)287Sato | ||
|---|---|---|---|
| Allele Name | transgene insertion 287, Thomas N Sato | ||
| Common Name(s) | TIE2-GFP; | ||
| Mutation Made By | Thomas Sato, Weill Medical College of Cornell Univ | ||
| Site of Expression | Blood vessel endothelial cells fluoresce green allowing the visualization of all vessels under fluorescent microscopy or purification of endothelial cells by FACS. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Tek, endothelial-specific receptor tyrosine kinase, mouse, laboratory | ||
| General Note | Endothelial cells in transgenic mice express GFP, and can be visualized via fluorescent microscopy or purified by FACS. | ||
| Molecular Note | The transgene contains Green Fluorescent Protein (GFP) under the control of the endothelial specific receptor tyrosine kinase (Tek, previous symbol Tie2) promoter. [J:93360] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Tg(TIE2GFP)287Sato
Colony Maintenance
| Breeding & Husbandry | Transgenic animals on the C57BL/6J background experience a 60% mortality rate in hemizygotes with the majority of death occurring at birth. Comparatively, this same transgenic line is available from The Jackson Laboratory on a STOCK background (stock number 003658), which is not subject to problems with mortality when maintained as a homozygote. Expected coat color: black |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (138 strains)
003658 STOCK Tg(TIE2GFP)287Sato/J
004128 B6.Cg-Tg(Tek-cre)12Flv/J 002856 FVB/N-Tg(TIE2-lacZ)182Sato/J 005707 STOCK Rag1tm1Mom Tg(TIE2-lacZ)182Sato/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedCardiovascular Research
Vascular Defects
Developmental Biology Research
Internal/Organ Defects (vasculature)
Endocrine Deficiency Research
Vasculature Defects
Research Tools
Cancer Research (endothelial cell marker for neovascularization)
Cardiovascular Research (endothelial cell marker for neovascularization)
Developmental Biology Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: endothelial cell markers for neovascularization)
Genetics Research (Tissue/Cell Markers: endothelial cells)
Immunology and Inflammation Research
Neurobiology Research
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesMotoike T; Loughna S; Perens E; Roman BL; Liao W; Chau TC; Richardson CD; Kawate T; Kuno J; Weinstein BM; Stainier DY; Sato TN. 2000. Universal GFP reporter for the study of vascular development. Genesis 28(2):75-81. [PubMed: 11064424] [J:93360]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(TIE2GFP)287Sato/1J |
| Stock Number: | 004659 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $307.40 | Hemizygous for Tg(TIE2GFP)287Sato | |||
| Pair | $375.30 | Hemizygous for Tg(TIE2GFP)287Sato x Noncarrier | |||
| Pair | $375.30 | Noncarrier x Hemizygous for Tg(TIE2GFP)287Sato | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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