Strain Name: |
PWD/PhJ |
|---|---|
Stock Number: |
004660 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
PWD/Ph (Changed: 15-DEC-04
) |
Type Inbred Strain Additional information on Inbred Strains. Type Wild-Derived Additional information on Wild-Derived Mice. Mating System Sibling x Sibling (Female x Male) Species M. m. musculus (Prague) H2 Haplotype ? Generation ?+F9F4 (05-DEC-07) Geographic Origin Kunratice, near Prague, the Czech Republic Appearance
agouti
Related Genotype: A/AStrain Description
PWD/PhJ is an inbred mouse strain of the subspecies Mus musculus musculus. M. m. musculus is estimated to have diverged approximately 1 million years ago from M. m. domesticus, the subspecies from which derives most of the genome of practically every laboratory mouse strain. However, the extent of polymorphism for randomly selected microsatellite markers between the two subspecies (70-80%) is nearly as great as between M. m. domesticus and Mus spretus (84%), which diverged about 3 million years ago (Montagutelli et al. 1991; Gergorova and Forejt 2000).PWD/Ph mice exhibit differences from mice of common laboratory strains for a number of complex phenotypic characteristics, including body mass, distribution of adipose tissue, serum concentrations of intermediary metabolites, susceptibility to type I diabetes and various behavioral traits (Gregorova and Forejt 2000).
Male, but not female, F1 hybrid offspring of crosses between mice of most laboratory strains and PWD/PhJ are sterile. Known exceptions are male (C3H x PWD)F1 and (CBA x PWD)F1 mice. The locus responsible for this difference in fertility of male PWD hybrids, called hybrid sterility 1 (symbol Hst1), has been mapped to a 580 kb region of M. m. domesticus Chr 17, approximately 8.4 cM distal to the centromere, wherein reside 10 potential candidate genes that are expressed in the testes (Forejt et al. 1991; Trachtulec et al. 1997). It has been estimated that no more than 3-4 loci contribute to the sterility phenotype of laboratory strain x M. m. musculus hybrid males (Gregorova and Forejt 2000).
Typing of PWD/Ph for 37 biochemical markers revealed numerous allelic differences with standard laboratory mouse strains (von Deimling et al. 1988). Electrophoretic alleles of 25 enzymes examined were shared between PWD and laboratory strains; nine of these markers were previously analyzed in wild-caught M. m. musculus mice, whose prevalent allele of all except one marker, Mod1, was fixed in PWD. PWD exhibited alleles at three loci that were uncommon among laboratory mice, but were identified in wild-caught M. m. musculus. Six esterase genes were identified in PWD that had also been detected in Mus molossinus but whose products were absent from laboratory strains. Unique alleles were identified in PWD for three enzymes.
Genome-wide microsatellite typing for 248 loci of PWD/Ph, PWK/Ph (distributed as PWK/PhJ, Stock No. 0003715) and/or PWB/Ph - very similar inbred strains derived from M. m. musculus pairs caught in the central Czech Republic - versus mice of three inbred laboratory strains revealed upto 80% of the loci to be polymorphic between at least one PW strain and at least one inbred strain analyzed.
The high level of both phenotypic variation and DNA sequence polymorphism between PWD/PhJ and inbred laboratory strains and the fertility of hybrid offspring of both sexes from crosses between PWD/PhJ and some laboratory strain mice make this strain an excellent tool for mapping both single-gene (Mendelian) traits and quantitative trait loci (QTL) contributing to complex phenotypes. Polymorphism at the RNA level permits investigation of monoallelic expression, e.g., from parentally imprinted genes (Forejt and Gregorova 1992; Forejt et al. 1999) or in cancer cell lines (Forejt et al. 1999). Sterility of male hybrid offspring of crosses between PWD and most inbred laboratory strains provides a tool for analysis of the genetic bases for interspecies hybrid sterility.
Strain Development
PWD/Ph is an inbred mouse strain descended from a single pair of mice of the subspecies Mus musculus musculus caught in 1972 in the "Kunratice" locality near Prague, in the central part of the Czech Repubic. The founder mice were selected on the basis of their producing sterile male offspring in matings with C57BL/10J mice. The strain was maintained from its inception by sister-brother inbreeding (Gregorova and Forejt 2000). In July 2003, PWD/Ph mice, then at generation F81, were donated to The Jackson Laboratory by Dr. Jiri Forejt, at the Institute for Molecular Genetics, Academy of Sciences of the Czech Republic, Prague. The colony at The Jackson Laboratory is descended from 8 female and 2 male mice from Dr. Forejt's colony.
| Breeding & Husbandry | These mice are still very wild after 80 generations of inbreeding, and special care is needed to prevent their escape during handling. PWD/Ph mice breed well. Females produce their first litters at about 8 weeks of age and will give birth approximately every 4 weeks. The average litter size is 7. Females continue breeding until at least 8 months old; the average number of litters produced during their reproductive lifetimes has been reported to be 6-7. |
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| Diet Information | LabDiet® 5K52/5K67 |
Mouse Phenome Database
Festing Inbred Strain Characteristics: PWD
JAX Notes, Winter 2006; 504. JAX® Mice: the Gold Standard Just Got Better.
Room Number AX11
Reproductive Biology Research
Fertility Defects (male progeny from outcrosses to inbred are sterile)
Research Tools
Genetics Research (Gene Mapping: Polymorphisms)
Selected Reference(s)
Additional ReferencesChubb C; Nolan C. 1987. Mouse hybrid sterility and testicular function. Biol Reprod 36(5):1343-8. [PubMed: 3620567] [J:109944]
Gregorova S; Forejt J. 2000. PWD/Ph and PWK/Ph inbred mouse strains of Mus m. musculus subspecies--a valuable resource of phenotypic variations and genomic polymorphisms. Folia Biol (Praha) 46(1):31-41. [PubMed: 10730880] [J:62922]
von Deimling OH; Forejt J; Wienker TF. 1988. Allelic profile at 37 biochemical loci of two inbred strains of the house mouse derived from wild Mus musculus musculus. Lab Anim 22(1):61-6. [PubMed: 3352221] [J:9097]
von Deimling OH; Groning J; Gaa A. 1991. Genetic and biochemical characterization of a new chymotrypsin isozyme of the house mouse, CTRA-1. Biochem Genet 29(11-12):545-57. [PubMed: 1820020] [J:1547]
| Strain Name: | PWD/PhJ |
| Stock Number: | 004660 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Mouse Mutant Resource collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below |
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