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Strain Name:

B6.Cg-Tg(PDGFB-APP)5Lms/J

Stock Number:

004662

Availability:

Repository- Live

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Genes & Alleles   APP;   PDGFB;   Tg(PDGFB-APP)5Lms;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating SystemHemizygote x Inbred         (Female x Male)
Specieslaboratory mouse
Donating Investigator Lennart Mucke,   Gladstone Inst of Neurological Disease
GenerationN11+11 (31-DEC-07)

Strain Description
These transgenic mice express a wildtype human amyloid protein precursor (APP) under the control of the human platelet-derived growth factor beta polypeptide (PDGFB) promoter. PCR primer modification was used to alter the sequence of the APPInd mutation to the wildtype sequence in this transgene. Hemizygotes express immunodetectable transgene product in cerebral neurons, with the highest level of expression occurring in the neocortex and hippocampus. Enzyme-linked immunosorbent assay (ELISA) analysis of neocortical and hippocampal tissue reveals approximate total amyloid beta peptides levels and 42 amino acid length amyloid beta peptides levels that are lower than levels found in the APP SwInd mutant line. No amyloid plaques are detected by immunohistochemistry at 8-10 months of age or at 24 months of age. Mutants display age dependent decrease in density of synaptophysin-immunoreactive presynaptic terminals indicative of neurodegeneration. This strain serves as the control for Stock No. 006293.

Strain Development
The APPInd transgene sequence was changed using PCR primer modification to the wildtype sequence. The transgenic construct containing the reverted wildtype human amyloid protein precursor, APP, under the control of human platelet-derived growth factor beta polypeptide, simian sarcoma viral (v-sis) oncogene homolog, (PDGFB) promoter, was injected into C57BL/6 X DBA/2 F2 one-cell embryos. Heterozygous founder animals were bred to C57BL/6 X DBA/2 F1 mice.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(PDGFB-APP)5Lms/?

        involves: C57BL/6 * DBA/2
  • nervous system phenotype
  • *normal* nervous system phenotype (MGI Ref ID J:62290)
    • no amyloid plaques are detected by immunohistochemistry up to the age of 24 months
    • neurodegeneration (MGI Ref ID J:62290)
      • neurodegeneration is indicated by an age dependent decrease in density of synaptophysin-immunoreactive presynaptic terminals

Gene & Allele Details

Allele Symbol Tg(PDGFB-APP)5Lms
Allele Name transgene insertion 5, Lennart Mucke
Common Name(s) I5; PDGF-APPWT; hAPPWT; line I5;
Mutation Made By Lennart Mucke,   Gladstone Inst of Neurological Disease
Strain of Origin(C57BL/6 x DBA/2)F2
Expressed Gene APP, amyloid beta (A4) precursor protein (peptidase nexin-II, Alzheimer disease), human
Promoter PDGFB, platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog), human
Molecular Note The transgene contains the reverted wild type human amyloid protein precursor, APP, under the control of the human platelet derived growth factor, B polypeptide, (PDGFB) promoter. Hemizygous transgenic mice express immunodetectable transgene product in cerebral neurons, with the highest level of expression occurring in the neocortex and hippocampus. ELISA analysis of neocortical and hippocampal tissue revealed approximate total amyloid beta peptides levels and 42 amino acid length amyloid beta peptides levels that were lower than levels found in mice carrying the mutant human amyloid protein precursor transgene Tg(PDGFB-APPSwInd)20Lms. [MGI Ref ID J:62290]

Control Information

   
   This strain serves as a control for Stock No. 006293.
 
  Considerations for Choosing Controls

Genotyping Protocols

PDGFB-APP

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying other alleles of APP     (15 strains)

Strains carrying other alleles of PDGFB
004661   B6.Cg-Tg(PDGFB-APPSwInd)20Lms/1J
006293   B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J
View Strains carrying other alleles of PDGFB     (2 strains)

Additional Web Information

Congenic Nomenclature
JAX Notes, Summer 2004; 494. New Mouse Models for Alzheimer's Disease Research.
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

APP related

Mouse/Human Gene Homologs
Alzheimer's

Neurobiology Research
Neurodegeneration

Tg(PDGFB-APP)5Lms related

Neurobiology Research
Alzheimer's Disease

References

Selected Reference(s)

Mucke L; Masliah E; Yu GQ; Mallory M; Rockenstein EM; Tatsuno G; Hu K; Kholodenko D; Johnson-Wood K; McConlogue L. 2000. High-level neuronal expression of abeta 1-42 in wild-type human amyloid protein precursor transgenic mice: synaptotoxicity without plaque formation. J Neurosci 20(11):4050-8. [PubMed: 10818140]  [MGI Ref ID J:62290]

Additional References

Price and Supply Information

Strain Name: B6.Cg-Tg(PDGFB-APP)5Lms/J
Stock Number: 004662

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE only available to non-profit entities.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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