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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Hemizygote x Inbred (Female x Male) 01-MAR-06 Species laboratory mouse Generation N11+13 (24-DEC-08)
Generation DefinitionsDonating Investigator Lennart Mucke, Gladstone Inst of Neurological Disease Description
These transgenic mice express a wildtype human amyloid protein precursor (APP) under the control of the human platelet-derived growth factor beta polypeptide (PDGFB) promoter. PCR primer modification was used to alter the sequence of the APPInd mutation to the wildtype sequence in this transgene. Hemizygotes express immunodetectable transgene product in cerebral neurons, with the highest level of expression occurring in the neocortex and hippocampus. Enzyme-linked immunosorbent assay (ELISA) analysis of neocortical and hippocampal tissue reveals approximate total amyloid beta peptides levels and 42 amino acid length amyloid beta peptides levels that are lower than levels found in the APP SwInd mutant line. No amyloid plaques are detected by immunohistochemistry at 8-10 months of age or at 24 months of age. Mutants display age dependent decrease in density of synaptophysin-immunoreactive presynaptic terminals indicative of neurodegeneration. This strain serves as the control for Stock No. 006293.Development
The APPInd transgene sequence was changed using PCR primer modification to the wildtype sequence. The transgenic construct containing the reverted wildtype human amyloid protein precursor, APP, under the control of human platelet-derived growth factor beta polypeptide, simian sarcoma viral (v-sis) oncogene homolog, (PDGFB) promoter, was injected into C57BL/6 X DBA/2 F2 one-cell embryos. Heterozygous founder animals were bred to C57BL/6 X DBA/2 F1 mice.
Control Information
| Control | ||
|---|---|---|
| See control note: | This strain serves as a control for Stock No. 006293. | |
| Considerations for Choosing Controls | ||
Related Strains
Alzheimer's Disease Models
View Alzheimer's Disease Models (108 strains)
006293 B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2Mmjax 016575 B6;C3-Tg(PDGFB-LRRK2*G2019S)340Djmo/J 016576 B6;C3-Tg(PDGFB-LRRK2*R1441C)574Djmo/J 008073 FVB-Tg(PDGFB-Adra2b)13Hag/J
Additional Web Information
JAX® NOTES, Summer 2004; 494. New Mouse Models for Alzheimer's Disease Research.
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(PDGFB-APP)5Lms/?
involves: C57BL/6 * DBA/2
- nervous system phenotype
- *normal* nervous system phenotype
- no amyloid plaques are detected by immunohistochemistry up to the age of 24 months (MGI Ref ID J:62290)
- amyloid beta deposits
- neurodegeneration
- neurodegeneration is indicated by an age dependent decrease in density of synaptophysin-immunoreactive presynaptic terminals (MGI Ref ID J:62290)
- other phenotype
- amyloid beta deposits
This mouse can be used to support research in many areas including:APP related
Tg(PDGFB-APP)5Lms relatedMouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Neurodegeneration
Neurobiology Research
Alzheimer's Disease
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(PDGFB-APP)5Lms | ||
|---|---|---|---|
| Allele Name | transgene insertion 5, Lennart Mucke | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | I5; PDGF-APPWT; hAPPWT; line I5; | ||
| Mutation Made By | Lennart Mucke, Gladstone Inst of Neurological Disease | ||
| Strain of Origin | (C57BL/6 x DBA/2)F2 | ||
| Expressed Gene | APP, amyloid beta (A4) precursor protein, human | ||
| Promoter | PDGFB, platelet-derived growth factor beta polypeptide, human | ||
| Molecular Note | The transgene contains the reverted wild-type human amyloid protein precursor, APP, under the control of the human platelet derived growth factor, B polypeptide, (PDGFB) promoter. Hemizygous transgenic mice express immunodetectable transgene product in cerebral neurons, with the highest level of expression occurring in the neocortex and hippocampus. ELISA analysis of neocortical and hippocampal tissue revealed approximate total amyloid beta peptides levels and 42 amino acid length amyloid beta peptides levels that were lower than levels found in mice carrying the mutant human amyloid protein precursor transgene Tg(PDGFB-APPSwInd)20Lms. [MGI Ref ID J:62290] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(PDGFB-APP) MCA, Melt Curve Analysis
Tg(PDGFB-APP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Mucke L; Masliah E; Yu GQ; Mallory M; Rockenstein EM; Tatsuno G; Hu K; Kholodenko D; Johnson-Wood K; McConlogue L. 2000. High-level neuronal expression of abeta 1-42 in wild-type human amyloid protein precursor transgenic mice: synaptotoxicity without plaque formation. J Neurosci 20(11):4050-8. [PubMed: 10818140] [MGI Ref ID J:62290]
Additional References
Tg(PDGFB-APP)5Lms relatedChin J; Massaro CM; Palop JJ; Thwin MT; Yu GQ; Bien-Ly N; Bender A; Mucke L. 2007. Reelin depletion in the Enthorhinal cortex of human amyloid precursor protein transgenic mice and humans with Alzheimer's disease J Neurosci 27(11):2727-2733. [PubMed: 17360894] [MGI Ref ID J:118589]
Ho RC; Fujii N; Witters LA; Hirshman MF; Goodyear LJ. 2007. Dissociation of AMP-activated protein kinase and p38 mitogen-activated protein kinase signaling in skeletal muscle. Biochem Biophys Res Commun 362(2):354-9. [PubMed: 17709097] [MGI Ref ID J:125174]
Hook VY; Kindy M; Reinheckel T; Peters C; Hook G. 2009. Genetic cathepsin B deficiency reduces beta-amyloid in transgenic mice expressing human wild-type amyloid precursor protein. Biochem Biophys Res Commun 386(2):284-8. [PubMed: 19501042] [MGI Ref ID J:151423]
Palop JJ; Chin J; Roberson ED; Wang J; Thwin MT; Bien-Ly N; Yoo J; Ho KO; Yu GQ; Kreitzer A; Finkbeiner S; Noebels JL; Mucke L. 2007. Aberrant excitatory neuronal activity and compensatory remodeling of inhibitory hippocampal circuits in mouse models of Alzheimer's disease. Neuron 55(5):697-711. [PubMed: 17785178] [MGI Ref ID J:126808]
Seeger G; Gartner U; Ueberham U; Rohn S; Arendt T. 2009. FAD-mutation of APP is associated with a loss of its synaptotrophic activity. Neurobiol Dis 35(2):258-63. [PubMed: 19450683] [MGI Ref ID J:150433]
Simon AM; Schiapparelli L; Salazar-Colocho P; Cuadrado-Tejedor M; Escribano L; Lopez de Maturana R; Del Rio J; Perez-Mediavilla A; Frechilla D. 2009. Overexpression of wild-type human APP in mice causes cognitive deficits and pathological features unrelated to Abeta levels. Neurobiol Dis 33(3):369-78. [PubMed: 19101630] [MGI Ref ID J:146301]
Thirumangalakudi L; Prakasam A; Zhang R; Bimonte-Nelson H; Sambamurti K; Kindy MS; Bhat NR. 2008. High cholesterol-induced neuroinflammation and amyloid precursor protein processing correlate with loss of working memory in mice. J Neurochem 106(1):475-85. [PubMed: 18410513] [MGI Ref ID J:137264]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Mating System Hemizygote x Inbred (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(PDGFB-APP)5Lms
Pairs /Price (US dollars $) Pair Genotype $280.40 C57BL/6J (000664) x Hemizygous for Tg(PDGFB-APP)5Lms $279.00 Hemizygous for Tg(PDGFB-APP)5Lms x C57BL/6J (000664) Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(PDGFB-APP)5Lms
Pairs /Price (US dollars $) Pair Genotype $364.60 C57BL/6J (000664) x Hemizygous for Tg(PDGFB-APP)5Lms $362.70 Hemizygous for Tg(PDGFB-APP)5Lms x C57BL/6J (000664) Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| See control note: | This strain serves as a control for Stock No. 006293. | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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