Strain Name: |
B6.Cg-Tg(PDGFB-APP)5Lms/J |
|---|---|
Stock Number: |
004662 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | APP; PDGFB; Tg(PDGFB-APP)5Lms; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Hemizygote x Inbred (Female x Male) Species laboratory mouse Donating Investigator Lennart Mucke, Gladstone Inst of Neurological Disease Generation N11+11 (31-DEC-07) Strain Description
These transgenic mice express a wildtype human amyloid protein precursor (APP) under the control of the human platelet-derived growth factor beta polypeptide (PDGFB) promoter. PCR primer modification was used to alter the sequence of the APPInd mutation to the wildtype sequence in this transgene. Hemizygotes express immunodetectable transgene product in cerebral neurons, with the highest level of expression occurring in the neocortex and hippocampus. Enzyme-linked immunosorbent assay (ELISA) analysis of neocortical and hippocampal tissue reveals approximate total amyloid beta peptides levels and 42 amino acid length amyloid beta peptides levels that are lower than levels found in the APP SwInd mutant line. No amyloid plaques are detected by immunohistochemistry at 8-10 months of age or at 24 months of age. Mutants display age dependent decrease in density of synaptophysin-immunoreactive presynaptic terminals indicative of neurodegeneration. This strain serves as the control for Stock No. 006293.Strain Development
The APPInd transgene sequence was changed using PCR primer modification to the wildtype sequence. The transgenic construct containing the reverted wildtype human amyloid protein precursor, APP, under the control of human platelet-derived growth factor beta polypeptide, simian sarcoma viral (v-sis) oncogene homolog, (PDGFB) promoter, was injected into C57BL/6 X DBA/2 F2 one-cell embryos. Heterozygous founder animals were bred to C57BL/6 X DBA/2 F1 mice.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Tg(PDGFB-APP)5Lms | ||
|---|---|---|---|
| Allele Name | transgene insertion 5, Lennart Mucke | ||
| Common Name(s) | I5; PDGF-APPWT; hAPPWT; line I5; | ||
| Mutation Made By | Lennart Mucke, Gladstone Inst of Neurological Disease | ||
| Strain of Origin | (C57BL/6 x DBA/2)F2 | ||
| Expressed Gene | APP, amyloid beta (A4) precursor protein (peptidase nexin-II, Alzheimer disease), human | ||
| Promoter | PDGFB, platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog), human | ||
| Molecular Note | The transgene contains the reverted wild type human amyloid protein precursor, APP, under the control of the human platelet derived growth factor, B polypeptide, (PDGFB) promoter. Hemizygous transgenic mice express immunodetectable transgene product in cerebral neurons, with the highest level of expression occurring in the neocortex and hippocampus. ELISA analysis of neocortical and hippocampal tissue revealed approximate total amyloid beta peptides levels and 42 amino acid length amyloid beta peptides levels that were lower than levels found in mice carrying the mutant human amyloid protein precursor transgene Tg(PDGFB-APPSwInd)20Lms. [MGI Ref ID J:62290] | ||
| This strain serves as a control for Stock No. 006293. | ||
| Considerations for Choosing Controls | ||
PDGFB-APP
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Strains carrying other alleles of APP
006409 129S1.Cg-Tg(APPSw)40Btla/J 006555 A.129(B6)-Tg(APPSw)40Btla/J 005300 B6.129-Tg(APPSw)40Btla/J 005301 B6.129S2-Tg(APP)8.9Btla/J 006406 B6.129S4-Tg(APPSwLon)96Btla/J 005864 B6.Cg-Tg(APPswe,PSEN1dE9)85Dbo/J 004661 B6.Cg-Tg(PDGFB-APPSwInd)20Lms/1J 006293 B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J 006006 B6.Cg-Tg(Prnp-APP)A-2Dbo/J 006005 B6.Cg-Tg(Prnp-App/APPswe)E1-2Dbo/J 007049 B6.Cg-Tg(tetO-APPSwInd)885Dbo/J 004462 B6C3-Tg(APPswe,PSEN1dE9)85Dbo/J 006004 B6C3-Tg(tetO-APPSwInd)885Dbo/J 007027 C57BL/6-Tg(Thy1-APPSwDutIowa)BWevn/J 006472 D2.129(B6)-Tg(APPSw)40Btla/J View Strains carrying other alleles of APP (15 strains)
Strains carrying other alleles of PDGFB
004661 B6.Cg-Tg(PDGFB-APPSwInd)20Lms/1J 006293 B6.Cg-Tg(PDGFB-APPSwInd)20Lms/2J View Strains carrying other alleles of PDGFB (2 strains)
Congenic Nomenclature
JAX Notes, Summer 2004; 494. New Mouse Models for Alzheimer's Disease Research.
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Room Number AX12
APP related
Tg(PDGFB-APP)5Lms relatedMouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Neurodegeneration
Neurobiology Research
Alzheimer's Disease
Selected Reference(s)
Additional ReferencesMucke L; Masliah E; Yu GQ; Mallory M; Rockenstein EM; Tatsuno G; Hu K; Kholodenko D; Johnson-Wood K; McConlogue L. 2000. High-level neuronal expression of abeta 1-42 in wild-type human amyloid protein precursor transgenic mice: synaptotoxicity without plaque formation. J Neurosci 20(11):4050-8. [PubMed: 10818140] [MGI Ref ID J:62290]
| Strain Name: | B6.Cg-Tg(PDGFB-APP)5Lms/J |
| Stock Number: | 004662 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE only available to non-profit entities.
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