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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.Cg-Tg(cre/Esr1)5Amc/J (Changed: 14-JUL-08 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Inbred x Hemizygote (Female x Male) 20-AUG-09 Species laboratory mouse Generation N6F1N8 (18-AUG-09) Donating Investigator IMR Colony, The Jackson Laboratory Description
These transgenic mice have a tamoxifen-inducible cre-mediated recombination system driven by the chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice. Homozygous mice are not viable or fertile. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A transgenic construct was designed with a cre/Esr1 fusion gene under the control of a chicken beta actin promoter/enhancer coupled with the cytomegalovirus (CMV) immediate-early enhancer. This transgene was introduced into B6CBF1 donor eggs. The resulting male transgenic mice were first backcrossed for 2 generations on the SWR background. Next, mice were backcrossed onto the C57BL/6J background for 5 generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(CAG-cre/Esr1)5Amc allele
004453 STOCK Tg(CAG-cre/Esr1)5Amc/J View Strains carrying Tg(CAG-cre/Esr1)5Amc (1 strain)
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 004744 B6.129P2-Esr1tm1Ksk/J 005657 B6.Cg-Tg(Myh6-cre/Esr1)1Jmk/J 005650 B6;129-Tg(Myh6-cre/Esr1)1Jmk/J 006774 FVB-Tg(Col2a1-cre/ESR1)KA3Smac/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 008464 STOCK Foxa2tm2.1(cre/Esr1)Moon/J 008122 STOCK Tg(Ins2-cre/Esr1)1Dam/J 005107 STOCK Tg(KRT14-cre/Esr1)20Efu/J 008119 STOCK Tg(Neurog3-cre/Esr1)1Dam/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(CAG-cre/Esr1)5Amc/?
involves: C57BL/6 * CBA
- tumorigenesis
- *normal* tumorigenesis (MGI Ref ID J:114992)
- no tumors are observed up to 12 months of age
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression: Inducible
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(CAG-cre/Esr1)5Amc | ||
|---|---|---|---|
| Allele Name | transgene insertion 5, Andrew P McMahon | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | CAG-CreERT2; CAGG-CreERT2; CAGGCre-ER; CAGGCre-ERTM; CAGGCre-ERtm line 5.8; CAGGS-CreER; CAGGS-CreErT; CMV-creERT; Cre-ER; Cre-ERTM; ER-cre; Tg(cre/Esr1)5Amc; | ||
| Mutation Made By | Shigemi Hayashi, Columbia University | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | tamoxifen-inducible cre; widespread pattern of expression; tamoxifen administration will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | Esr1, estrogen receptor 1 (alpha), mouse, laboratory | ||
| Promoter | ACTB, actin, beta, chicken | ||
| Driver Note | CAG | ||
| General Note |
Homozygous transgenic mice are not viable or fertile. Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In transgenic mice the mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 fusion protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing loxP sites flanking a sequence of interest, tamoxifen induced, Cre-mediated targeted deletions are generated in the offspring. Tamoxifen administration will induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice. | ||
| Inducible Note | Induced by tamoxifen. | ||
| Molecular Note | This transgene expresses a fusion protein consisting of Cre recombinase joined to the ligand-binding domain of a mouse estrogen receptor modified to bind to 4-hydroxytamoxifen, but not to endogenous estrogen. The CAG promoter, containing a chicken beta actin promoter/enhancer coupled with the cytomegalovirus immediate-early (CMV-IE) enhancer, drives high levels of expression in most tissues. In the presence of tamoxifen, the fusion protein is transported into the nucleus, where Cre can excise loxP-flanked segments from conditionally modified genes. [MGI Ref ID J:76130] | ||
| Gene Symbol and Name | Tg(CAG-cre/Esr1)5Amc, transgene insertion 5, Andrew P McMahon | ||
| Chromosome | UN | ||
| Gene Common Name(s) | CAG-CreERT2; CAGG-CreERT2; CAGGCre-ER; CAGGCre-ERTM; CAGGCre-ERtm line 5.8; CAGGS-CreER; CAGGS-CreErT; CMV-creERT; Cre-ER; Cre-ERTM; ER-cre; Tg(cre/Esr1)5Amc; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Hayashi S; McMahon AP. 2002. Efficient recombination in diverse tissues by a tamoxifen-inducible form of cre: a tool for temporally regulated gene activation/inactivation in the mouse. Dev Biol 244(2):305-18. [PubMed: 11944939] [MGI Ref ID J:76130]
Additional References
Tg(CAG-cre/Esr1)5Amc relatedBhaskara S; Chyla BJ; Amann JM; Knutson SK; Cortez D; Sun ZW; Hiebert SW. 2008. Deletion of histone deacetylase 3 reveals critical roles in S phase progression and DNA damage control. Mol Cell 30(1):61-72. [PubMed: 18406327] [MGI Ref ID J:134665]
Brenner-Anantharam A; Cebrian C; Guillaume R; Hurtado R; Sun TT; Herzlinger D. 2007. Tailbud-derived mesenchyme promotes urinary tract segmentation via BMP4 signaling. Development 134(10):1967-75. [PubMed: 17442697] [MGI Ref ID J:121412]
Briggs LE; Takeda M; Cuadra AE; Wakimoto H; Marks MH; Walker AJ; Seki T; Oh SP; Lu JT; Sumners C; Raizada MK; Horikoshi N; Weinberg EO; Yasui K; Ikeda Y; Chien KR; Kasahara H. 2008. Perinatal loss of Nkx2-5 results in rapid conduction and contraction defects. Circ Res 103(6):580-90. [PubMed: 18689573] [MGI Ref ID J:143802]
Brydges SD; Mueller JL; McGeough MD; Pena CA; Misaghi A; Gandhi C; Putnam CD; Boyle DL; Firestein GS; Horner AA; Soroosh P; Watford WT; O'Shea JJ; Kastner DL; Hoffman HM. 2009. Inflammasome-mediated disease animal models reveal roles for innate but not adaptive immunity. Immunity 30(6):875-87. [PubMed: 19501000] [MGI Ref ID J:150054]
Cai J; Chen Y; Cai WH; Hurlock EC; Wu H; Kernie SG; Parada LF; Lu QR. 2007. A crucial role for Olig2 in white matter astrocyte development. Development 134(10):1887-99. [PubMed: 17428828] [MGI Ref ID J:121422]
Chan G; Kalaitzidis D; Usenko T; Kutok JL; Yang W; Mohi MG; Neel BG. 2009. Leukemogenic Ptpn11 causes fatal myeloproliferative disorder via cell-autonomous effects on multiple stages of hematopoiesis. Blood 113(18):4414-24. [PubMed: 19179468] [MGI Ref ID J:148430]
Chang H; Gao F; Guillou F; Taketo MM; Huff V; Behringer RR. 2008. Wt1 negatively regulates {beta}-catenin signaling during testis development. Development 135(10):1875-85. [PubMed: 18403409] [MGI Ref ID J:134687]
Cheng J; Du J. 2007. Mechanical stretch simulates proliferation of venous smooth muscle cells through activation of the insulin-like growth factor-1 receptor. Arterioscler Thromb Vasc Biol 27(8):1744-51. [PubMed: 17541019] [MGI Ref ID J:134907]
Cho JH; Mu X; Wang SW; Klein WH. 2009. Retinal ganglion cell death and optic nerve degeneration by genetic ablation in adult mice. Exp Eye Res 88(3):542-52. [PubMed: 19109949] [MGI Ref ID J:146558]
Connerney J; Andreeva V; Leshem Y; Mercado MA; Dowell K; Yang X; Lindner V; Friesel RE; Spicer DB. 2008. Twist1 homodimers enhance FGF responsiveness of the cranial sutures and promote suture closure. Dev Biol 318(2):323-34. [PubMed: 18471809] [MGI Ref ID J:136965]
Fukuda S; Singh P; Moh A; Abe M; Conway EM; Boswell HS; Yamaguchi S; Fu XY; Pelus LM. 2009. Survivin mediates aberrant hematopoietic progenitor cell proliferation and acute leukemia in mice induced by internal tandem duplication of Flt3. Blood 114(2):394-403. [PubMed: 19411632] [MGI Ref ID J:150766]
Garrett AM; Weiner JA. 2009. Control of CNS synapse development by {gamma}-protocadherin-mediated astrocyte-neuron contact. J Neurosci 29(38):11723-31. [PubMed: 19776259] [MGI Ref ID J:153046]
Gould TW; Yonemura S; Oppenheim RW; Ohmori S; Enomoto H. 2008. The neurotrophic effects of glial cell line-derived neurotrophic factor on spinal motoneurons are restricted to fusimotor subtypes. J Neurosci 28(9):2131-46. [PubMed: 18305247] [MGI Ref ID J:132854]
Guy J; Gan J; Selfridge J; Cobb S; Bird A. 2007. Reversal of neurological defects in a mouse model of Rett syndrome. Science 315(5815):1143-7. [PubMed: 17289941] [MGI Ref ID J:118365]
Herrmann S; Stieber J; Stockl G; Hofmann F; Ludwig A. 2007. HCN4 provides a 'depolarization reserve' and is not required for heart rate acceleration in mice. EMBO J 26(21):4423-32. [PubMed: 17914461] [MGI Ref ID J:139560]
Higgins DF; Kimura K; Bernhardt WM; Shrimanker N; Akai Y; Hohenstein B; Saito Y; Johnson RS; Kretzler M; Cohen CD; Eckardt KU; Iwano M; Haase VH. 2007. Hypoxia promotes fibrogenesis in vivo via HIF-1 stimulation of epithelial-to-mesenchymal transition. J Clin Invest 117(12):3810-20. [PubMed: 18037992] [MGI Ref ID J:130751]
Jacoby M; Cox JJ; Gayral S; Hampshire DJ; Ayub M; Blockmans M; Pernot E; Kisseleva MV; Compere P; Schiffmann SN; Gergely F; Riley JH; Perez-Morga D; Woods CG; Schurmans S. 2009. INPP5E mutations cause primary cilium signaling defects, ciliary instability and ciliopathies in human and mouse. Nat Genet 41(9):1027-31. [PubMed: 19668215] [MGI Ref ID J:152712]
Ji B; Song J; Tsou L; Bi Y; Gaiser S; Mortensen R; Logsdon C. 2008. Robust acinar cell transgene expression of CreErT via BAC recombineering. Genesis 46(8):390-5. [PubMed: 18693271] [MGI Ref ID J:148748]
Johnson NC; Dillard ME; Baluk P; McDonald DM; Harvey NL; Frase SL; Oliver G. 2008. Lymphatic endothelial cell identity is reversible and its maintenance requires Prox1 activity. Genes Dev 22(23):3282-91. [PubMed: 19056883] [MGI Ref ID J:142041]
Knapp JR; Davie JK; Myer A; Meadows E; Olson EN; Klein WH. 2006. Loss of myogenin in postnatal life leads to normal skeletal muscle but reduced body size. Development 133(4):601-10. [PubMed: 16407395] [MGI Ref ID J:105925]
Kobayashi T; Lu J; Cobb BS; Rodda SJ; McMahon AP; Schipani E; Merkenschlager M; Kronenberg HM. 2008. Dicer-dependent pathways regulate chondrocyte proliferation and differentiation. Proc Natl Acad Sci U S A 105(6):1949-54. [PubMed: 18238902] [MGI Ref ID J:131828]
Lepper C; Conway SJ; Fan CM. 2009. Adult satellite cells and embryonic muscle progenitors have distinct genetic requirements. Nature 460(7255):627-31. [PubMed: 19554048] [MGI Ref ID J:150962]
Li G; Adesnik H; Li J; Long J; Nicoll RA; Rubenstein JL; Pleasure SJ. 2008. Regional distribution of cortical interneurons and development of inhibitory tone are regulated by Cxcl12/Cxcr4 signaling. J Neurosci 28(5):1085-98. [PubMed: 18234887] [MGI Ref ID J:131835]
List K; Kosa P; Szabo R; Bey AL; Wang CB; Molinolo A; Bugge TH. 2009. Epithelial integrity is maintained by a matriptase-dependent proteolytic pathway. Am J Pathol 175(4):1453-63. [PubMed: 19717635] [MGI Ref ID J:153070]
Liu W; Lagutin OV; Mende M; Streit A; Oliver G. 2006. Six3 activation of Pax6 expression is essential for mammalian lens induction and specification. EMBO J 25(22):5383-95. [PubMed: 17066077] [MGI Ref ID J:116102]
Mao J; Ligon KL; Rakhlin EY; Thayer SP; Bronson RT; Rowitch D; McMahon AP. 2006. A novel somatic mouse model to survey tumorigenic potential applied to the Hedgehog pathway. Cancer Res 66(20):10171-8. [PubMed: 17047082] [MGI Ref ID J:114992]
Matsuki T; Pramatarova A; Howell BW. 2008. Reduction of Crk and CrkL expression blocks reelin-induced dendritogenesis. J Cell Sci 121(Pt 11):1869-75. [PubMed: 18477607] [MGI Ref ID J:139781]
Matsunobu T; Torigoe K; Ishikawa M; de Vega S; Kulkarni AB; Iwamoto Y; Yamada Y. 2009. Critical roles of the TGF-beta type I receptor ALK5 in perichondrial formation and function, cartilage integrity, and osteoblast differentiation during growth plate development. Dev Biol 332(2):325-38. [PubMed: 19501582] [MGI Ref ID J:152864]
Meadows E; Cho JH; Flynn JM; Klein WH. 2008. Myogenin regulates a distinct genetic program in adult muscle stem cells. Dev Biol 322(2):406-414. [PubMed: 18721801] [MGI Ref ID J:141955]
Minamishima YA; Moslehi J; Bardeesy N; Cullen D; Bronson RT; Kaelin WG Jr. 2008. Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure. Blood 111(6):3236-44. [PubMed: 18096761] [MGI Ref ID J:132718]
Mudhasani R; Zhu Z; Hutvagner G; Eischen CM; Lyle S; Hall LL; Lawrence JB; Imbalzano AN; Jones SN. 2008. Loss of miRNA biogenesis induces p19Arf-p53 signaling and senescence in primary cells. J Cell Biol 181(7):1055-63. [PubMed: 18591425] [MGI Ref ID J:139257]
Nishide K; Nakatani Y; Kiyonari H; Kondo T. 2009. Glioblastoma formation from cell population depleted of Prominin1-expressing cells. PLoS One 4(8):e6869. [PubMed: 19718438] [MGI Ref ID J:152390]
Oda H; Okamoto I; Murphy N; Chu J; Price SM; Shen MM; Torres-Padilla ME; Heard E; Reinberg D. 2009. Monomethylation of histone H4-lysine 20 is involved in chromosome structure and stability and is essential for mouse development. Mol Cell Biol 29(8):2278-95. [PubMed: 19223465] [MGI Ref ID J:147765]
Paek H; Gutin G; Hebert JM. 2009. FGF signaling is strictly required to maintain early telencephalic precursor cell survival. Development 136(14):2457-65. [PubMed: 19542358] [MGI Ref ID J:150347]
Park SG; Schulze-Luehrman J; Hayden MS; Hashimoto N; Ogawa W; Kasuga M; Ghosh S. 2009. The kinase PDK1 integrates T cell antigen receptor and CD28 coreceptor signaling to induce NF-kappaB and activate T cells. Nat Immunol 10(2):158-66. [PubMed: 19122654] [MGI Ref ID J:144512]
Pascual A; Hidalgo-Figueroa M; Piruat JI; Pintado CO; Gomez-Diaz R; Lopez-Barneo J. 2008. Absolute requirement of GDNF for adult catecholaminergic neuron survival. Nat Neurosci 11(7):755-61. [PubMed: 18536709] [MGI Ref ID J:139371]
Paul E; Cronan R; Weston PJ; Boekelheide K; Sedivy JM; Lee SY; Wiest DL; Resnick MB; Klysik JE. 2009. Disruption of Supv3L1 damages the skin and causes sarcopenia, loss of fat, and death. Mamm Genome 20(2):92-108. [PubMed: 19145458] [MGI Ref ID J:144991]
Penzo-Mendez A; Dy P; Pallavi B; Lefebvre V. 2007. Generation of mice harboring a Sox4 conditional null allele. Genesis 45(12):776-80. [PubMed: 18064674] [MGI Ref ID J:130461]
Perera D; Tilston V; Hopwood JA; Barchi M; Boot-Handford RP; Taylor SS. 2007. Bub1 maintains centromeric cohesion by activation of the spindle checkpoint. Dev Cell 13(4):566-79. [PubMed: 17925231] [MGI Ref ID J:128455]
Perry JM; Harandi OF; Porayette P; Hegde S; Kannan AK; Paulson RF. 2009. Maintenance of the BMP4-dependent stress erythropoiesis pathway in the murine spleen requires hedgehog signaling. Blood 113(4):911-8. [PubMed: 18927434] [MGI Ref ID J:144571]
Piontek K; Menezes LF; Garcia-Gonzalez MA; Huso DL; Germino GG. 2007. A critical developmental switch defines the kinetics of kidney cyst formation after loss of Pkd1. Nat Med 13(12):1490-5. [PubMed: 17965720] [MGI Ref ID J:141463]
Qin J; Tsai MJ; Tsai SY. 2008. Essential roles of COUP-TFII in Leydig cell differentiation and male fertility. PLoS ONE 3(9):e3285. [PubMed: 18818749] [MGI Ref ID J:144248]
Quignodon L; Vincent S; Winter H; Samarut J; Flamant F. 2007. A Point Mutation in the Activation Function 2 Domain of Thyroid Hormone Receptor {alpha}1 Expressed after CRE-Mediated Recombination Partially Recapitulates Hypothyroidism. Mol Endocrinol 21(10):2350-2360. [PubMed: 17622582] [MGI Ref ID J:125353]
Regard JB; Kataoka H; Cano DA; Camerer E; Yin L; Zheng YW; Scanlan TS; Hebrok M; Coughlin SR. 2007. Probing cell type-specific functions of Gi in vivo identifies GPCR regulators of insulin secretion. J Clin Invest 117(12):4034-43. [PubMed: 17992256] [MGI Ref ID J:130781]
Silk AD; Holland AJ; Cleveland DW. 2009. Requirements for NuMA in maintenance and establishment of mammalian spindle poles. J Cell Biol 184(5):677-90. [PubMed: 19255246] [MGI Ref ID J:146214]
Takeda M; Briggs LE; Wakimoto H; Marks MH; Warren SA; Lu JT; Weinberg EO; Robertson KD; Chien KR; Kasahara H. 2009. Slow progressive conduction and contraction defects in loss of Nkx2-5 mice after cardiomyocyte terminal differentiation. Lab Invest 89(9):983-93. [PubMed: 19546853] [MGI Ref ID J:151895]
Tang H; Macpherson P; Marvin M; Meadows E; Klein WH; Yang XJ; Goldman D. 2009. A histone deacetylase 4/myogenin positive feedback loop coordinates denervation-dependent gene induction and suppression. Mol Biol Cell 20(4):1120-31. [PubMed: 19109424] [MGI Ref ID J:153383]
Tokunaga A; Oya T; Ishii Y; Motomura H; Nakamura C; Ishizawa S; Fujimori T; Nabeshima Y; Umezawa A; Kanamori M; Kimura T; Sasahara M. 2008. PDGF receptor beta is a potent regulator of mesenchymal stromal cell function. J Bone Miner Res 23(9):1519-28. [PubMed: 18410236] [MGI Ref ID J:152593]
Uesaka T; Jain S; Yonemura S; Uchiyama Y; Milbrandt J; Enomoto H. 2007. Conditional ablation of GFRalpha1 in postmigratory enteric neurons triggers unconventional neuronal death in the colon and causes a Hirschsprung's disease phenotype. Development 134(11):2171-81. [PubMed: 17507417] [MGI Ref ID J:122607]
Uesaka T; Nagashimada M; Yonemura S; Enomoto H. 2008. Diminished Ret expression compromises neuronal survival in the colon and causes intestinal aganglionosis in mice. J Clin Invest 118(5):1890-8. [PubMed: 18414682] [MGI Ref ID J:135153]
Wang M; Crisostomo P; Wairiuko GM; Meldrum DR. 2006. Estrogen receptor-alpha mediates acute myocardial protection in females. Am J Physiol Heart Circ Physiol 290(6):H2204-9. [PubMed: 16415070] [MGI Ref ID J:111852]
Wang P; Bowl MR; Bender S; Peng J; Farber L; Chen J; Ali A; Zhang Z; Alberts AS; Thakker RV; Shilatifard A; Williams BO; Teh BT. 2008. Parafibromin, a component of the human PAF complex, regulates growth factors and is required for embryonic development and survival in adult mice. Mol Cell Biol 28(9):2930-40. [PubMed: 18212049] [MGI Ref ID J:135255]
Welle S; Bhatt K; Pinkert CA; Tawil R; Thornton CA. 2007. Muscle growth after postdevelopmental myostatin gene knockout. Am J Physiol Endocrinol Metab 292(4):E985-91. [PubMed: 17148752] [MGI Ref ID J:121233]
Xu H; Cerrato F; Baldini A. 2005. Timed mutation and cell-fate mapping reveal reiterated roles of Tbx1 during embryogenesis, and a crucial function during segmentation of the pharyngeal system via regulation of endoderm expansion. Development 132(19):4387-95. [PubMed: 16141220] [MGI Ref ID J:127213]
Xu H; Chen L; Baldini A. 2007. In vivo genetic ablation of the periotic mesoderm affects cell proliferation survival and differentiation in the cochlea. Dev Biol 310(2):329-40. [PubMed: 17825816] [MGI Ref ID J:128008]
Xu H; Viola A; Zhang Z; Gerken CP; Lindsay-Illingworth EA; Baldini A. 2007. Tbx1 regulates population, proliferation and cell fate determination of otic epithelial cells. Dev Biol 302(2):670-82. [PubMed: 17074316] [MGI Ref ID J:119950]
Yang B; Zhao D; Qian L; Verkman AS. 2006. Mouse model of inducible nephrogenic diabetes insipidus produced by floxed aquaporin-2 gene deletion. Am J Physiol Renal Physiol 291(2):F465-72. [PubMed: 16434568] [MGI Ref ID J:110648]
Yang B; Zhao D; Verkman AS. 2009. Hsp90 inhibitor partially corrects nephrogenic diabetes insipidus in a conditional knock-in mouse model of aquaporin-2 mutation. FASEB J 23(2):503-12. [PubMed: 18854434] [MGI Ref ID J:146024]
Yang X; Harkins LK; Zubanova O; Harrington A; Kovalenko D; Nadeau RJ; Chen PY; Toher JL; Lindner V; Liaw L; Friesel R. 2008. Overexpression of Spry1 in chondrocytes causes attenuated FGFR ubiquitination and sustained ERK activation resulting in chondrodysplasia. Dev Biol 321(1):64-76. [PubMed: 18582454] [MGI Ref ID J:138616]
Yingling J; Youn YH; Darling D; Toyo-Oka K; Pramparo T; Hirotsune S; Wynshaw-Boris A. 2008. Neuroepithelial stem cell proliferation requires LIS1 for precise spindle orientation and symmetric division. Cell 132(3):474-86. [PubMed: 18267077] [MGI Ref ID J:135521]
Young AP; Schlisio S; Minamishima YA; Zhang Q; Li L; Grisanzio C; Signoretti S; Kaelin WG Jr. 2008. VHL loss actuates a HIF-independent senescence programme mediated by Rb and p400. Nat Cell Biol 10(3):361-9. [PubMed: 18297059] [MGI Ref ID J:145670]
Yu PB; Deng DY; Lai CS; Hong CC; Cuny GD; Bouxsein ML; Hong DW; McManus PM; Katagiri T; Sachidanandan C; Kamiya N; Fukuda T; Mishina Y; Peterson RT; Bloch KD. 2008. BMP type I receptor inhibition reduces heterotopic ossification. Nat Med 14(12):1363-9. [PubMed: 19029982] [MGI Ref ID J:142253]
Yuan X; Yao J; Norris D; Tran DD; Bram RJ; Chen G; Luscher B. 2008. Calcium-modulating cyclophilin ligand regulates membrane trafficking of postsynaptic GABA(A) receptors. Mol Cell Neurosci 38(2):277-89. [PubMed: 18424167] [MGI Ref ID J:136913]
Zhang CL; Zou Y; He W; Gage FH; Evans RM. 2008. A role for adult TLX-positive neural stem cells in learning and behaviour. Nature 451(7181):1004-7. [PubMed: 18235445] [MGI Ref ID J:132664]
Zhang Y; Singh MK; Degenhardt KR; Lu MM; Bennett J; Yoshida Y; Epstein JA. 2009. Tie2Cre-mediated inactivation of plexinD1 results in congenital heart, vascular and skeletal defects. Dev Biol 325(1):82-93. [PubMed: 18992737] [MGI Ref ID J:143763]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, carrier mice may be bred with wildtype (noncarrier) mice from the colony. The expected coat color is black nonagouti. Mating System Inbred x Hemizygote (Female x Male) 20-AUG-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Hemizygous for Tg(CAG-cre/Esr1)5Amc
Pairs /Price (US dollars $) Pair Genotype $297.85 Noncarrier x Hemizygous for Tg(cre/Esr1)5Amc $297.85 Hemizygous for Tg(CAG-cre/Esr1)5Amc x Noncarrier
Additional Supply Details
| Pricing for International shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Hemizygous for Tg(CAG-cre/Esr1)5Amc
Pairs /Price (US dollars $) Pair Genotype $387.30 Noncarrier x Hemizygous for Tg(cre/Esr1)5Amc $387.30 Hemizygous for Tg(CAG-cre/Esr1)5Amc x Noncarrier
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.