Strain Name:

B6;129S4-Tsc2tm1Djk/J

Stock Number:

004686

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This Tsc2 KO mutant mouse strain may be useful in studies of Tuberous sclerosis.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator David J. Kwiatkowski,   Brigham and Women's Hospital

Description
Exon 2 of the tuberous sclerosis 2 (Tsc2) gene has been replaced by a neo cassette, abolishing gene expression. TSC2 is a putative tumor supressor. Mutation of TSC2 have been associated with the onset of tuberous sclerosis complex (TSC) which is characterized by the formation of non-malignant tumors in many different organs. Mice that are heterozygous for the targeted mutation are viable and fertile. Homozygous null mice have an embryonic lethal phenotype, failing to develop past embryonic days 9.5 to 12.5 due to hepatic hypoplasia. Cultured neuroepithelial progenitor cells isolated from embryonic day 10.5 embryos display abnormal growth and differentiation. All heterozygotes develop multiple bilateral renal cystadenomas by 12-15 months of age. By 15 months, about half develop liver hemangiomas (more common in females than in males). Less than 10% develop extremity angiosarcomas or renal carcinoma. Little or no gene product (protein) is detected by Western blot in renal cystadenomas. PCR analysis reveals loss of the wildtype allele in about 30% of lesions. Phenotype variability is dependent on genetic background.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 2 of the targeted gene. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.

Control Information

  Control
   Wild-type from the colony
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Tsc2
014564   C57BL/6-Tg(CMV-Tsc2*)1Arbi/KlanJ
View Strains carrying other alleles of Tsc2     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Tuberous Sclerosis 2; TSC2
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Lymphangioleiomyomatosis; LAM   (TSC2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tsc2tm1Djk/Tsc2+

        either: (involves: 129S4/SvJae * BALB/cJ) or (involves: 129S4/SvJae * Black Swiss) or (involves: 129S4/SvJae * C57BL/6J)
  • tumorigenesis
  • increased tumor incidence
    • reported at 15 months of age   (MGI Ref ID J:57631)
    • note that the tumor expression pattern is influenced by genetic background; authors note fewer large renal cystadenomas in outbred Black Swiss background and more angiosarcomas in 129S4/SvJae chimeric mice note that the tumor expression pattern is influenced by genetic background; authors note fewer large renal cystadenomas in outbred Black Swiss background and more angiosarcomas in 129S4/SvJae chimeric mice   (MGI Ref ID J:57631)
    • increased extremity angiosarcoma incidence
      • less than 10% incidence   (MGI Ref ID J:57631)
    • increased hepatic hemangioma incidence
      • 50% incidence; causes fatal bleeding in 10% of these cases   (MGI Ref ID J:57631)
    • increased lung adenoma incidence
      • 32% incidence   (MGI Ref ID J:57631)
    • increased renal carcinoma incidence
      • less than 10% incidence   (MGI Ref ID J:57631)
    • increased renal cystadenoma incidence
      • 100% incidence   (MGI Ref ID J:57631)

Tsc2tm1Djk/Tsc2+

        involves: 129S4/SvJae * C57BL/6NCrl
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • mice exhibit normal motor skills, exploratory behavior, anxiety and social approach behavior   (MGI Ref ID J:138621)
    • abnormal contextual conditioning behavior
      • following contextual conditioning, mice exhibit reduced freezing compared to wild-type mice   (MGI Ref ID J:138621)
      • however, treatment with rapamycin re-establishes normal contextual conditioning   (MGI Ref ID J:138621)
    • abnormal spatial learning
      • in a hippocampus-dependent version of the Morris water maze test, spatial learning is impaired compared to in wild-type mice   (MGI Ref ID J:138621)
      • however, spatial learning in a hippocampus-independent water maze is normal and treatment with rapamycin re-establishes normal spatial learning   (MGI Ref ID J:138621)
      • mice exhibit more across-phase errors compared to in wild-type mice in a hippocampus-dependent win-shift version of the eight-arm radial maze   (MGI Ref ID J:138621)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • mice exhibit normal basal synaptic transmission, paired-pulse facilitation and early-phase long term potentiation   (MGI Ref ID J:138621)
    • enhanced long term potentiation
      • mice exhibit lowered late-phase threshold for long term potentiation compared to in wild-type mice   (MGI Ref ID J:138621)
      • however, treatment with rapamycin re-establishes normal late-phase long term potentiation   (MGI Ref ID J:138621)

Tsc2tm1Djk/Tsc2tm1Djk

        either: (involves: 129S4/SvJae * BALB/cJ) or (involves: 129S4/SvJae * Black Swiss) or (involves: 129S4/SvJae * C57BL/6J)
  • mortality/aging
  • complete embryonic lethality during organogenesis
    • age of onset E9.5   (MGI Ref ID J:57631)
  • liver/biliary system phenotype
  • liver hypoplasia   (MGI Ref ID J:57631)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence
      Other Tissues/Organs: multiple

Developmental Biology Research
Embryonic Lethality (Homozygous)

Tsc2tm1Djk related

Cancer Research
Increased Tumor Incidence
      Adenomas
      Other Tissues/Organs
      Other Tissues/Organs: lung

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tsc2tm1Djk
Allele Name targeted mutation 1, David J Kwiatkowski
Allele Type Targeted (knock-out)
Common Name(s) Tsc2-; Tsc-;
Mutation Made By David Kwiatkowski,   Brigham and Women's Hospital
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Tsc2, tuberous sclerosis 2
Chromosome 17
Gene Common Name(s) LAM; Nafld; Rc; TSC4; tuberin;
Molecular Note A neomycin cassette was inserted into the second coding exon of the gene. [MGI Ref ID J:57631]

Genotyping

Genotyping Information

Genotyping Protocols

Tsc2tm1Djkalternate2,

SEPARATED MELT


Tsc2tm1Djkalternate2, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Onda H; Lueck A; Marks PW; Warren HB; Kwiatkowski DJ. 1999. Tsc2(+/-) mice develop tumors in multiple sites that express gelsolin and are influenced by genetic background. J Clin Invest 104(6):687-95. [PubMed: 10491404]  [MGI Ref ID J:57631]

Additional References

Uhlmann EJ; Apicelli AJ; Baldwin RL; Burke SP; Bajenaru ML; Onda H; Kwiatkowski D; Gutmann DH. 2002. Heterozygosity for the tuberous sclerosis complex (TSC) gene products results in increased astrocyte numbers and decreased p27-Kip1 expression in TSC2+/- cells. Oncogene 21(25):4050-9. [PubMed: 12037687]  [MGI Ref ID J:77179]

Tsc2tm1Djk related

Auerbach BD; Osterweil EK; Bear MF. 2011. Mutations causing syndromic autism define an axis of synaptic pathophysiology. Nature 480(7375):63-8. [PubMed: 22113615]  [MGI Ref ID J:178293]

Bae SH; Sung SH; Oh SY; Lim JM; Lee SK; Park YN; Lee HE; Kang D; Rhee SG. 2013. Sestrins activate Nrf2 by promoting p62-dependent autophagic degradation of Keap1 and prevent oxidative liver damage. Cell Metab 17(1):73-84. [PubMed: 23274085]  [MGI Ref ID J:195093]

Blando J; Portis M; Benavides F; Alexander A; Mills G; Dave B; Conti CJ; Kim J; Walker CL. 2009. PTEN deficiency is fully penetrant for prostate adenocarcinoma in C57BL/6 mice via mTOR-dependent growth. Am J Pathol 174(5):1869-79. [PubMed: 19395652]  [MGI Ref ID J:148013]

Bonnet CS; Aldred M; von Ruhland C; Harris R; Sandford R; Cheadle JP. 2009. Defects in cell polarity underlie TSC and ADPKD-associated cystogenesis. Hum Mol Genet 18(12):2166-76. [PubMed: 19321600]  [MGI Ref ID J:148543]

Brown AB; Mahmood U; Cortes ML; Tang Y; Dai G; Stemmer-Rachamimov A; Prabhakar S; Leishear K; Onda H; Kwiatkowski D; Weissleder R; Breakefield X. 2005. Magnetic resonance imaging and characterization of spontaneous lesions in a transgenic mouse model of tuberous sclerosis as a model for endothelial cell-based transgene delivery. Hum Gene Ther 16(12):1367-76. [PubMed: 16390268]  [MGI Ref ID J:107438]

Cao J; Gong L; Guo DC; Mietzsch U; Kuang SQ; Kwartler CS; Safi H; Estrera A; Gambello MJ; Milewicz DM. 2010. Thoracic aortic disease in tuberous sclerosis complex: molecular pathogenesis and potential therapies in Tsc2+/- mice. Hum Mol Genet 19(10):1908-20. [PubMed: 20159776]  [MGI Ref ID J:159339]

Dibble CC; Elis W; Menon S; Qin W; Klekota J; Asara JM; Finan PM; Kwiatkowski DJ; Murphy LO; Manning BD. 2012. TBC1D7 Is a Third Subunit of the TSC1-TSC2 Complex Upstream of mTORC1. Mol Cell 47(4):535-46. [PubMed: 22795129]  [MGI Ref ID J:187614]

Ehninger D; Han S; Shilyansky C; Zhou Y; Li W; Kwiatkowski DJ; Ramesh V; Silva AJ. 2008. Reversal of learning deficits in a Tsc2+/- mouse model of tuberous sclerosis. Nat Med 14(8):843-8. [PubMed: 18568033]  [MGI Ref ID J:138621]

Gilbert ER; Eby JM; Hammer AM; Klarquist J; Christensen DG; Barfuss AJ; Boissy RE; Picken MM; Love RB; Dilling DF; Le Poole IC. 2013. Positioning ganglioside d3 as an immunotherapeutic target in lymphangioleiomyomatosis. Am J Pathol 183(1):226-34. [PubMed: 23665200]  [MGI Ref ID J:197440]

Hartman TR; Liu D; Zilfou JT; Robb V; Morrison T; Watnick T; Henske EP. 2009. The tuberous sclerosis proteins regulate formation of the primary cilium via a rapamycin-insensitive and polycystin 1-independent pathway. Hum Mol Genet 18(1):151-63. [PubMed: 18845692]  [MGI Ref ID J:143404]

Huang J; Wu S; Wu CL; Manning BD. 2009. Signaling events downstream of mammalian target of rapamycin complex 2 are attenuated in cells and tumors deficient for the tuberous sclerosis complex tumor suppressors. Cancer Res 69(15):6107-14. [PubMed: 19602587]  [MGI Ref ID J:150957]

Kwiatkowski DJ; Zhang H; Bandura JL; Heiberger KM; Glogauer M; el-Hashemite N; Onda H. 2002. A mouse model of TSC1 reveals sex-dependent lethality from liver hemangiomas, and up-regulation of p70S6 kinase activity in Tsc1 null cells. Hum Mol Genet 11(5):525-34. [PubMed: 11875047]  [MGI Ref ID J:75243]

Lee L; Sudentas P; Donohue B; Asrican K; Worku A; Walker V; Sun Y; Schmidt K; Albert MS; El-Hashemite N; Lader AS; Onda H; Zhang H; Kwiatkowski DJ; Dabora SL. 2005. Efficacy of a rapamycin analog (CCI-779) and IFN-gamma in tuberous sclerosis mouse models. Genes Chromosomes Cancer 42(3):213-27. [PubMed: 15578690]  [MGI Ref ID J:95229]

Ma L; Teruya-Feldstein J; Behrendt N; Chen Z; Noda T; Hino O; Cordon-Cardo C; Pandolfi PP. 2005. Genetic analysis of Pten and Tsc2 functional interactions in the mouse reveals asymmetrical haploinsufficiency in tumor suppression. Genes Dev 19(15):1779-86. [PubMed: 16027168]  [MGI Ref ID J:100094]

Mak BC; Kenerson HL; Aicher LD; Barnes EA; Yeung RS. 2005. Aberrant beta-catenin signaling in tuberous sclerosis. Am J Pathol 167(1):107-16. [PubMed: 15972957]  [MGI Ref ID J:99509]

Manning BD; Logsdon MN; Lipovsky AI; Abbott D; Kwiatkowski DJ; Cantley LC. 2005. Feedback inhibition of Akt signaling limits the growth of tumors lacking Tsc2. Genes Dev 19(15):1773-8. [PubMed: 16027169]  [MGI Ref ID J:100095]

Nie D; Di Nardo A; Han JM; Baharanyi H; Kramvis I; Huynh T; Dabora S; Codeluppi S; Pandolfi PP; Pasquale EB; Sahin M. 2010. Tsc2-Rheb signaling regulates EphA-mediated axon guidance. Nat Neurosci 13(2):163-72. [PubMed: 20062052]  [MGI Ref ID J:156683]

Obayashi Y; Campbell JS; Fausto N; Yeung RS. 2013. Impaired lipid accumulation in the liver of Tsc2-heterozygous mice during liver regeneration. Biochem Biophys Res Commun 437(1):146-50. [PubMed: 23810393]  [MGI Ref ID J:205324]

Onda H; Crino PB; Zhang H; Murphey RD; Rastelli L; Gould Rothberg BE; Kwiatkowski DJ. 2002. Tsc2 Null Murine Neuroepithelial Cells Are a Model for Human Tuber Giant Cells, and Show Activation of an mTOR Pathway. Mol Cell Neurosci 21(4):561-74. [PubMed: 12504590]  [MGI Ref ID J:81245]

Parkhitko A; Myachina F; Morrison TA; Hindi KM; Auricchio N; Karbowniczek M; Wu JJ; Finkel T; Kwiatkowski DJ; Yu JJ; Henske EP. 2011. Tumorigenesis in tuberous sclerosis complex is autophagy and p62/sequestosome 1 (SQSTM1)-dependent. Proc Natl Acad Sci U S A 108(30):12455-60. [PubMed: 21746920]  [MGI Ref ID J:174534]

Pollizzi K; Malinowska-Kolodziej I; Doughty C; Betz C; Ma J; Goto J; Kwiatkowski DJ. 2009. A hypomorphic allele of Tsc2 highlights the role of TSC1/TSC2 in signaling to AKT and models mild human TSC2 alleles. Hum Mol Genet 18(13):2378-87. [PubMed: 19357198]  [MGI Ref ID J:149326]

Pollizzi K; Malinowska-Kolodziej I; Stumm M; Lane H; Kwiatkowski D. 2009. Equivalent benefit of mTORC1 blockade and combined PI3K-mTOR blockade in a mouse model of tuberous sclerosis. Mol Cancer 8:38. [PubMed: 19527517]  [MGI Ref ID J:157342]

Potter WB; Basu T; O'Riordan KJ; Kirchner A; Rutecki P; Burger C; Roopra A. 2013. Reduced juvenile long-term depression in tuberous sclerosis complex is mitigated in adults by compensatory recruitment of mGluR5 and Erk signaling. PLoS Biol 11(8):e1001627. [PubMed: 23966835]  [MGI Ref ID J:201589]

Settembre C; Zoncu R; Medina DL; Vetrini F; Erdin S; Erdin S; Huynh T; Ferron M; Karsenty G; Vellard MC; Facchinetti V; Sabatini DM; Ballabio A. 2012. A lysosome-to-nucleus signalling mechanism senses and regulates the lysosome via mTOR and TFEB. EMBO J 31(5):1095-108. [PubMed: 22343943]  [MGI Ref ID J:182149]

Short JD; Houston KD; Dere R; Cai SL; Kim J; Johnson CL; Broaddus RR; Shen J; Miyamoto S; Tamanoi F; Kwiatkowski D; Mills GB; Walker CL. 2008. AMP-activated protein kinase signaling results in cytoplasmic sequestration of p27. Cancer Res 68(16):6496-506. [PubMed: 18701472]  [MGI Ref ID J:139145]

Uhlmann EJ; Apicelli AJ; Baldwin RL; Burke SP; Bajenaru ML; Onda H; Kwiatkowski D; Gutmann DH. 2002. Heterozygosity for the tuberous sclerosis complex (TSC) gene products results in increased astrocyte numbers and decreased p27-Kip1 expression in TSC2+/- cells. Oncogene 21(25):4050-9. [PubMed: 12037687]  [MGI Ref ID J:77179]

Wang CY; X738b X798e X9685; Stapleton DS; Schueler KL; Rabaglia ME; Oler AT; Keller MP; Kendziorski CM; Broman KW; Yandell BS; Schadt EE; Attie AD. 2012. Tsc2, a positional candidate gene underlying a quantitative trait locus for hepatic steatosis. J Lipid Res 53(8):1493-1501. [PubMed: 22628617]  [MGI Ref ID J:186560]

Yang J; Kalogerou M; Gallacher J; Sampson JR; Shen MH. 2013. Renal tumours in a Tsc1+/- mouse model show epigenetic suppression of organic cation transporters Slc22a1, Slc22a2 and Slc22a3, and do not respond to metformin. Eur J Cancer 49(6):1479-90. [PubMed: 23228442]  [MGI Ref ID J:196914]

Yuan E; Tsai PT; Greene-Colozzi E; Sahin M; Kwiatkowski DJ; Malinowska IA. 2012. Graded loss of tuberin in an allelic series of brain models of TSC correlates with survival, and biochemical, histological and behavioral features. Hum Mol Genet 21(19):4286-300. [PubMed: 22752306]  [MGI Ref ID J:187405]

Zhang H; Cicchetti G; Onda H; Koon HB; Asrican K; Bajraszewski N; Vazquez F; Carpenter CL; Kwiatkowski DJ. 2003. Loss of Tsc1/Tsc2 activates mTOR and disrupts PI3K-Akt signaling through downregulation of PDGFR. J Clin Invest 112(8):1223-33. [PubMed: 14561707]  [MGI Ref ID J:162300]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThe resulting chimeric animals were crossed to C57BL/6 mice. Heterozygotes were then intercrossed. Expected coat color is: Black

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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