Strain Name: |
B6;FVB-Tg(Pcp2-EGFP)2Yuza/J |
|---|---|
Stock Number: |
004690 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
B6;FVB-Tg(Pcp2-EGFP)146.244Yuza/J (Changed: 09-MAY-05
) |
| Genes & Alleles | GFP; Pcp2; Tg(Pcp2-EGFP)2Yuza; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Hemizygote x Noncarrier (Female x Male) Species laboratory mouse Donating Investigator IMR Colony, The Jackson Laboratory Generation N?+4F6 (19-DEC-07) Strain Description
These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the direction of the mouse Purkinje cell protein 2 promoter. Mice hemizygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Transgenic mice display distinct expression of EGFP in dendrites, axons and soma of Purkinje cells, allowing identification, isolation and purification of Purkinje cells by FACS. EGFP expression mimics endogenous Purkinje cell protein 2 expression pattern. Fluorescence is detectable at embryonic day 17 in Purkinje cells and also occurs in retinal bipolar cells. Low levels of fluorescence are seen in olfactory periglomerular cells, interpeduncular nucleus and superior colliculus neurons. This mutant mouse strain represents a model that may be useful for purification and in vivo tracking of Purkinje cells.Strain Development
A transgenic construct containing an Enhanced Green Fluorescent Protein (EGFP, Clontech) gene under the control of a mouse Purkinje cell protein 2 promoter was injected into fertilized FVB/N mouse eggs. The donating investigator intercrossed founder lines 146 and 244 prior to the strain's arrival at The Jackson Laboratory. During the development of the production stock at The Jackson Laboratory, the founder insert with the larger number of transgene copies was retained as two more backcrosses to C57BL/6J were performed. It is unknown if the retained insert originated from founder line 146 or 244.
Gene & Allele Details
| Allele Symbol | Tg(Pcp2-EGFP)2Yuza | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Michisuke Yuzaki | ||
| Common Name(s) | L7-GFP; | ||
| Mutation Made By | Michisuke Yuzaki, Keio University School of Medicine | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Dendrites, axons, and soma of Purkinje cells express GFP. GFP fluorescence is detectable at embryonic day 17 in Purkinje cells and also occurs in retinal bipolar cells. Low levels of GFP are seen in olfactory periglomerular cells, interpeduncular nucleus, and superior colliculus neurons. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Pcp2, Purkinje cell protein 2 (L7), mouse, laboratory | ||
| General Note | Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These mice display distinct expression of GFP in dendrites, axons and soma of Purkinje cells, allowing identification, isolation, and purification of Purkinje cells by FACS. GFP expression mimics endogenous Purkinje cell protein 2 expression pattern. Fluorescence is detectable at embryonic day 17 in Purkinje cells and also occurs in retinal bipolar cells. Low levels of fluorescence are seen in olfactory periglomerular cells, interpeduncular nucleus, and superior colliculus neurons. | ||
| Molecular Note | The transgene contains an Enhanced Green Fluorescent Protein gene under the control of the mouse Purkinje cell protein 2 promoter. [J:93484] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes. |
|---|
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (138 strains)
004146 B6.129-Tg(Pcp2-cre)2Mpin/J 005625 FVB-Tg(Pcp2-tTA)3Horr/J 006207 STOCK Tg(Pcp2-cre)1Amc/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesTomomura M; Rice DS; Morgan JI; Yuzaki M. 2001. Purification of Purkinje cells by fluorescence-activated cell sorting from transgenic mice that express green fluorescent protein. Eur J Neurosci 14(1):57-63. [PubMed: 11488949] [J:93484]
Price and Supply Information
| Strain Name: | B6;FVB-Tg(Pcp2-EGFP)2Yuza/J |
| Stock Number: | 004690 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $307.40 | Hemizygous for Tg(Pcp2-EGFP)2Yuza | |||
| Pair | $375.30 | Hemizygous for Tg(Pcp2-EGFP)2Yuza x Noncarrier | |||
| Pair | $375.30 | Noncarrier x Hemizygous for Tg(Pcp2-EGFP)2Yuza | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-207-288-5845. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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