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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names STOCK Tg(Hoxb7-cre)13Amc (Changed: 15-DEC-04 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N1F2+F1p (19-FEB-06)
Generation DefinitionsDonating Investigator Andrew McMahon, Harvard University Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are not viable. These transgenic mice express the Cre recombinase under the control of the mouse homeobox B7 enhancer and promoter. Recombination closely patterns the endogenous gene expression. Cre recombinase expression is detected in the mesonephric duct as early as embryonic day 9.5, in the ureteric bud by embryonic day 10.25 and in all ureteric bud epithelial cells by embryonic day 12.5. Low levels of expression are detected in the dorsal root ganglia and the spinal cord. When crossed with a strain containing a loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in the mesonephric duct and its developmental derivatives (the Wolffian duct, the collecting duct epithelium of kidney and ureteral epithelium). This strain represents an effective tool for generating tissue-specific targeted mutants that would be useful in examining developmentally critical gene function in renal system development.View cre expression characterization.
Development
A transgenic construct containing the 1.3Kb enhancer and promoter sequence upstream of the of mouse homeo box B7 gene, a human growth factor mini gene and the Cre coding sequence was injected into fertilized C57BL/6 mouse eggs.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Hoxb7
016251 129S.Cg-Tg(Hoxb7-EGFP)33Cos/J 016567 129S.Cg-Tg(Hoxb7-rtTA*M2)2Cos/J 016252 STOCK Tg(Hoxb7-Venus*)17Cos/J View Strains carrying other alleles of Hoxb7 (3 strains)
Additional Web Information
Genetic Quality Control Annual Report
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Hoxb7-cre)13Amc | ||
|---|---|---|---|
| Allele Name | transgene insertion 13, Andrew P McMahon | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | HoxB7/Cre; HoxB7Cre; Hoxb7-Cre; | ||
| Mutation Made By | Tom Carroll, Harvard University | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | mesonephric duct and its developmental derivatives (the Wolffian duct, the collecting duct epithelium of kidney and ureteral epithelium), ureteric bud and all ureteric bud epithelial cells; low levels of expression in the dorsal root ganglia and the spinal cord | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Hoxb7, homeobox B7, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Hoxb7-cre)13Amc, transgene insertion 13, Andrew P McMahon | ||
| Chromosome | UN | ||
| Gene Common Name(s) | HoxB7/Cre; HoxB7Cre; | ||
| Driver Note | Hoxb7 | ||
| Molecular Note | A transgenic construct containing the 1.3Kb enhancer and promoter sequence upstream of the of mouse homeo box B7 gene, a human growth factor mini gene and the Cre coding sequence was injected into fertilized C57BL/6 mouse eggs. Cre recombinase expression is detected in the mesonephric duct as early as embryonic day 9.5, in the ureteric bud by embryonic day 10.25 and in all ureteric bud epithelial cells by embryonic day 12.5. Low levels of expression are detected in the dorsal root ganglia and the spinalcord. [MGI Ref ID J:100575] [MGI Ref ID J:79481] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Hoxb7-cre)13Amc, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Yu J; Carroll TJ; McMahon AP. 2002. Sonic hedgehog regulates proliferation and differentiation of mesenchymal cells in the mouse metanephric kidney. Development 129(22):5301-12. [PubMed: 12399320] [MGI Ref ID J:79481]
Additional References
Li C; Xiao J; Hormi K; Borok Z; Minoo P. 2002. Wnt5a participates in distal lung morphogenesis. Dev Biol 248(1):68-81. [PubMed: 12142021] [MGI Ref ID J:78384]
Tg(Hoxb7-cre)13Amc relatedBasson MA; Akbulut S; Watson-Johnson J; Simon R; Carroll TJ; Shakya R; Gross I; Martin GR; Lufkin T; McMahon AP; Wilson PD; Costantini FD; Mason IJ; Licht JD. 2005. Sprouty1 is a critical regulator of GDNF/RET-mediated kidney induction. Dev Cell 8(2):229-39. [PubMed: 15691764] [MGI Ref ID J:96485]
Basson MA; Watson-Johnson J; Shakya R; Akbulut S; Hyink D; Costantini FD; Wilson PD; Mason IJ; Licht JD. 2006. Branching morphogenesis of the ureteric epithelium during kidney development is coordinated by the opposing functions of GDNF and Sprouty1. Dev Biol 299(2):466-77. [PubMed: 17022962] [MGI Ref ID J:115303]
Carroll TJ; Park JS; Hayashi S; Majumdar A; McMahon AP. 2005. Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system. Dev Cell 9(2):283-92. [PubMed: 16054034] [MGI Ref ID J:100575]
Chia I; Grote D; Marcotte M; Batourina E; Mendelsohn C; Bouchard M. 2011. Nephric duct insertion is a crucial step in urinary tract maturation that is regulated by a Gata3-Raldh2-Ret molecular network in mice. Development 138(10):2089-97. [PubMed: 21521737] [MGI Ref ID J:171426]
Fenton RA; Moeller HB; Hoffert JD; Yu MJ; Nielsen S; Knepper MA. 2008. Acute regulation of aquaporin-2 phosphorylation at Ser-264 by vasopressin. Proc Natl Acad Sci U S A 105(8):3134-9. [PubMed: 18287043] [MGI Ref ID J:132818]
Genetic Resource Sciences at The Jackson Laboratory. 2008. Expression/Specificity patterns of Cre transgenes MGI Direct Data Submission :. [MGI Ref ID J:137887]
Grote D; Boualia SK; Souabni A; Merkel C; Chi X; Costantini F; Carroll T; Bouchard M. 2008. Gata3 acts downstream of beta-catenin signaling to prevent ectopic metanephric kidney induction. PLoS Genet 4(12):e1000316. [PubMed: 19112489] [MGI Ref ID J:142876]
Humphreys BD; Lin SL; Kobayashi A; Hudson TE; Nowlin BT; Bonventre JV; Valerius MT; McMahon AP; Duffield JS. 2010. Fate tracing reveals the pericyte and not epithelial origin of myofibroblasts in kidney fibrosis. Am J Pathol 176(1):85-97. [PubMed: 20008127] [MGI Ref ID J:158051]
Ishibe S; Karihaloo A; Ma H; Zhang J; Marlier A; Mitobe M; Togawa A; Schmitt R; Czyczk J; Kashgarian M; Geller DS; Thorgeirsson SS; Cantley LG. 2009. Met and the epidermal growth factor receptor act cooperatively to regulate final nephron number and maintain collecting duct morphology. Development 136(2):337-45. [PubMed: 19103805] [MGI Ref ID J:143511]
Jeong HW; Jeon US; Koo BK; Kim WY; Im SK; Shin J; Cho Y; Kim J; Kong YY. 2009. Inactivation of Notch signaling in the renal collecting duct causes nephrogenic diabetes insipidus in mice. J Clin Invest 119(11):3290-300. [PubMed: 19855135] [MGI Ref ID J:154589]
Jonassen JA; San Agustin J; Follit JA; Pazour GJ. 2008. Deletion of IFT20 in the mouse kidney causes misorientation of the mitotic spindle and cystic kidney disease. J Cell Biol 183(3):377-84. [PubMed: 18981227] [MGI Ref ID J:141071]
Karner CM; Dietrich MF; Johnson EB; Kappesser N; Tennert C; Percin F; Wollnik B; Carroll TJ; Herz J. 2010. Lrp4 regulates initiation of ureteric budding and is crucial for kidney formation--a mouse model for Cenani-Lenz syndrome. PLoS One 5(4):e10418. [PubMed: 20454682] [MGI Ref ID J:160547]
Kobayashi A; Kwan KM; Carroll TJ; McMahon AP; Mendelsohn CL; Behringer RR. 2005. Distinct and sequential tissue-specific activities of the LIM-class homeobox gene Lim1 for tubular morphogenesis during kidney development. Development 132(12):2809-23. [PubMed: 15930111] [MGI Ref ID J:98831]
Kovacikova J; Winter C; Loffing-Cueni D; Loffing J; Finberg KE; Lifton RP; Hummler E; Rossier B; Wagner CA. 2006. The connecting tubule is the main site of the furosemide-induced urinary acidification by the vacuolar H+-ATPase. Kidney Int 70(10):1706-16. [PubMed: 16985514] [MGI Ref ID J:136484]
Kuure S; Chi X; Lu B; Costantini F. 2010. The transcription factors Etv4 and Etv5 mediate formation of the ureteric bud tip domain during kidney development. Development 137(12):1975-9. [PubMed: 20463033] [MGI Ref ID J:161409]
Leviel F; Hubner CA; Houillier P; Morla L; El Moghrabi S; Brideau G; Hatim H; Parker MD; Kurth I; Kougioumtzes A; Sinning A; Pech V; Riemondy KA; Miller RL; Hummler E; Shull GE; Aronson PS; Doucet A; Wall SM; Chambrey R; Eladari D. 2010. The Na+-dependent chloride-bicarbonate exchanger SLC4A8 mediates an electroneutral Na+ reabsorption process in the renal cortical collecting ducts of mice. J Clin Invest 120(5):1627-35. [PubMed: 20389022] [MGI Ref ID J:161474]
Ma H; Saenko M; Opuko A; Togawa A; Soda K; Marlier A; Moeckel GW; Cantley LG; Ishibe S. 2009. Deletion of the Met receptor in the collecting duct decreases renal repair following ureteral obstruction. Kidney Int 76(8):868-76. [PubMed: 19675527] [MGI Ref ID J:166323]
Marose TD; Merkel CE; McMahon AP; Carroll TJ. 2008. Beta-catenin is necessary to keep cells of ureteric bud/Wolffian duct epithelium in a precursor state. Dev Biol 314(1):112-26. [PubMed: 18177851] [MGI Ref ID J:131012]
Mugford JW; Sipila P; Kobayashi A; Behringer RR; McMahon AP. 2008. Hoxd11 specifies a program of metanephric kidney development within the intermediate mesoderm of the mouse embryo. Dev Biol 319(2):396-405. [PubMed: 18485340] [MGI Ref ID J:137524]
Olsen O; Funke L; Long JF; Fukata M; Kazuta T; Trinidad JC; Moore KA; Misawa H; Welling PA; Burlingame AL; Zhang M; Bredt DS. 2007. Renal defects associated with improper polarization of the CRB and DLG polarity complexes in MALS-3 knockout mice. J Cell Biol 179(1):151-64. [PubMed: 17923534] [MGI Ref ID J:134806]
Pastorelli LM; Wells S; Fray M; Smith A; Hough T; Harfe BD; McManus MT; Smith L; Woolf AS; Cheeseman M; Greenfield A. 2009. Genetic analyses reveal a requirement for Dicer1 in the mouse urogenital tract. Mamm Genome 20(3):140-51. [PubMed: 19169742] [MGI Ref ID J:146873]
Reginensi A; Clarkson M; Neirijnck Y; Lu B; Ohyama T; Groves AK; Sock E; Wegner M; Costantini F; Chaboissier MC; Schedl A. 2011. SOX9 controls epithelial branching by activating RET effector genes during kidney development. Hum Mol Genet 20(6):1143-53. [PubMed: 21212101] [MGI Ref ID J:168845]
Rojek A; Fuchtbauer EM; Kwon TH; Frokiaer J; Nielsen S. 2006. Severe urinary concentrating defect in renal collecting duct-selective AQP2 conditional-knockout mice. Proc Natl Acad Sci U S A 103(15):6037-42. [PubMed: 16581908] [MGI Ref ID J:108291]
Rosselot C; Spraggon L; Chia I; Batourina E; Riccio P; Lu B; Niederreither K; Dolle P; Duester G; Chambon P; Costantini F; Gilbert T; Molotkov A; Mendelsohn C. 2010. Non-cell-autonomous retinoid signaling is crucial for renal development. Development 137(2):283-92. [PubMed: 20040494] [MGI Ref ID J:157254]
Rubera I; Loffing J; Palmer LG; Frindt G; Fowler-Jaeger N; Sauter D; Carroll T; McMahon A; Hummler E; Rossier BC. 2003. Collecting duct-specific gene inactivation of alphaENaC in the mouse kidney does not impair sodium and potassium balance. J Clin Invest 112(4):554-65. [PubMed: 12925696] [MGI Ref ID J:85156]
Smeeton J; Zhang X; Bulus N; Mernaugh G; Lange A; Karner CM; Carroll TJ; Fassler R; Pozzi A; Rosenblum ND; Zent R. 2010. Integrin-linked kinase regulates p38 MAPK-dependent cell cycle arrest in ureteric bud development. Development 137(19):3233-43. [PubMed: 20823064] [MGI Ref ID J:165815]
Willecke R; Heuberger J; Grossmann K; Michos O; Schmidt-Ott K; Walentin K; Costantini F; Birchmeier W. 2011. The tyrosine phosphatase Shp2 acts downstream of GDNF/Ret in branching morphogenesis of the developing mouse kidney. Dev Biol 360(2):310-7. [PubMed: 22015719] [MGI Ref ID J:178713]
Wu W; Kitamura S; Truong DM; Rieg T; Vallon V; Sakurai H; Bush KT; Vera DR; Ross RS; Nigam SK. 2009. {beta}1-Integrin is required for kidney collecting duct morphogenesis and maintenance of renal function. Am J Physiol Renal Physiol 297(1):F210-7. [PubMed: 19439520] [MGI Ref ID J:149831]
Yu J; Carroll TJ; Rajagopal J; Kobayashi A; Ren Q; McMahon AP. 2009. A Wnt7b-dependent pathway regulates the orientation of epithelial cell division and establishes the cortico-medullary axis of the mammalian kidney. Development 136(1):161-71. [PubMed: 19060336] [MGI Ref ID J:142685]
Zhang X; Mernaugh G; Yang DH; Gewin L; Srichai MB; Harris RC; Iturregui JM; Nelson RD; Kohan DE; Abrahamson D; Fassler R; Yurchenco P; Pozzi A; Zent R. 2009. beta1 integrin is necessary for ureteric bud branching morphogenesis and maintenance of collecting duct structural integrity. Development 136(19):3357-66. [PubMed: 19710172] [MGI Ref ID J:153635]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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