Strain Name: |
STOCK Tg(Hoxb7-cre)13Amc/J |
|---|---|
Stock Number: |
004692 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
STOCK Tg(Hoxb7-cre)13Amc (Changed: 15-DEC-04
) |
| Genes & Alleles | Hoxb7; cre; Tg(Hoxb7-cre)13Amc; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Andrew McMahon, Harvard University Generation N1F2+F1p (19-FEB-06) Strain Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are not viable. These transgenic mice express the Cre recombinase under the control of the mouse homeobox B7 enhancer and promoter. Recombination closely patterns the endogenous gene expression. Cre recombinase expression is detected in the mesonephric duct as early as embryonic day 9.5, in the ureteric bud by embryonic day 10.25 and in all ureteric bud epithelial cells by embryonic day 12.5. Low levels of expression are detected in the dorsal root ganglia and the spinal cord. When crossed with a strain containing a loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in the mesonephric duct and its developmental derivatives (the Wolffian duct, the collecting duct epithelium of kidney and ureteral epithelium). This strain represents an effective tool for generating tissue-specific targeted mutants that would be useful in examining developmentally critical gene function in renal system development.Strain Development
A transgenic construct containing the 1.3Kb enhancer and promoter sequence upstream of the of mouse homeo box B7 gene, a human growth factor mini gene and the Cre coding sequence was injected into fertilized C57BL/6 mouse eggs.
Gene & Allele Details
| Allele Symbol | Tg(Hoxb7-cre)13Amc | ||
|---|---|---|---|
| Allele Name | transgene insertion 13, Andrew P McMahon | ||
| Common Name(s) | HoxB7/Cre; HoxB7Cre; | ||
| Mutation Made By | Tom Carroll, Harvard University | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | mesonephric duct and its developmental derivatives (the Wolffian duct, the collecting duct epithelium of kidney and ureteral epithelium), ureteric bud and all ureteric bud epithelial cells; low levels of expression in the dorsal root ganglia and the spinal cord | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Hoxb7, homeo box B7, mouse, laboratory | ||
| Molecular Note | A transgenic construct containing the 1.3Kb enhancer and promoter sequence upstream of the of mouse homeo box B7 gene, a human growth factor mini gene and the Cre coding sequence was injected into fertilized C57BL/6 mouse eggs. Cre recombinase expression is detected in the mesonephric duct as early as embryonic day 9.5, in the ureteric bud by embryonic day 10.25 and in all ureteric bud epithelial cells by embryonic day 12.5. Low levels of expression are detected in the dorsal root ganglia and the spinalcord. [MGI Ref ID J:100575] [MGI Ref ID J:79481] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Hoxb7-cre)13Amc
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (115 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Genetic Quality Control Annual Report
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesYu J; Carroll TJ; McMahon AP. 2002. Sonic hedgehog regulates proliferation and differentiation of mesenchymal cells in the mouse metanephric kidney. Development 129(22):5301-12. [PubMed: 12399320] [MGI Ref ID J:79481]
Price and Supply Information
| Strain Name: | STOCK Tg(Hoxb7-cre)13Amc/J |
| Stock Number: | 004692 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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