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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse H2 Haplotype g7 Generation N9F12p (16-MAY-04) Donating Investigator David Serreze, The Jackson Laboratory Appearance
albino
Related Genotype: A/A Tyrc/TyrcDevelopment
Zinkernagel et al, established a set of LCMV-specific cytotoxic T-cell clones from lethally irradiated B10.BR/SgSn(H-2K) reconstituted with T-cell depleted C57BL/10Sn ({/i H-2b}) bone marrow cells. The variable beta chain from clone P14, containing Tcrb-V8.1, TcrB-D, TcrB-J2.4 gene segments under the control of the H-2Kb promoter and variable alpha chain from the P14 clone, containing Tcra-V2 and Tcra-J TA31 gene segments under the control of the H-2Kb promoter were co-injected into B6D2F2 fertilized embryos. 10-20 copies of both the alpha and beta transgenes co-integrated on the same chromosome in male founder number 327. Founder 327 was backcrossed to C57BL/6J for one generation prior to being intercrossed to make this a homozygous strain. Serezze, et al. obtained JR2331-B6;D2-Tg(TcrLCMV)327Sdz/J mice, backcrossed to NOD/Lt for 9 generations and then mated Tg/? x Tg/?. A genome scan for NOD derived Idd loci was done as described in J. Exp. Med. 1996 184:2049. This T cell receptor is specific for an LCMV peptide in the context of H2-Db and requires the presence of H2-Dbto be positively selected. (Serreze et al., Diabetes, 2001.) In 2003, the T1DR received NOD.Cg-Tg(TcrLCMV)327Sdz/DvsJ from Dr. Serezze. This strain is maintained Tg/? x Tg/?
Control Information
| Control | ||
|---|---|---|
| 004694 B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(TcrLCMV)327Sdz allele
004694 B6;D2-Tg(TcrLCMV)327Sdz/JDvsJ 004257 NOD.Cg-Prkdcscid Tg(TcrLCMV)327Sdz/Dvs View Strains carrying Tg(TcrLCMV)327Sdz (2 strains)
Additional Web Information
Congenic Nomenclature
Phenotype
Phenotype Information
assigned by genotype
Tg(TcrLCMV)327Sdz/Tg(TcrLCMV)327Sdz
NOD.Cg-Tg(TcrLCMV)327Sdz/Dvs
- immune system phenotype
- autoimmune response (MGI Ref ID J:71050)
- the rate and frequency of type 1 diabetes development in transgenic NOD females is the same as that in NOD females lacking the transgene
This mouse can be used to support research in many areas including:
Tcra relatedResearch Tools
Immunology and Inflammation Research (T Cell Receptor Transgenics)
Tcrb relatedHematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Research Tools
Cancer Research (specific T cell deficiency)
Hematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(TcrLCMV)327Sdz | ||
|---|---|---|---|
| Allele Name | transgene insertion 327, Birgit Ledermann | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | P14; P14 TCR; P14 TCR-alphabeta (Valpha2Vbeta8.1); P14 TCRValpha2Vbeta8; P14-TCR; TCR LCMV; TCR P14; TCR-Tg; TgTcr; | ||
| Mutation Made By | Rolf Zinkernagel, University of Zurich | ||
| Strain of Origin | (C57BL/6 x DBA/2)F2 | ||
| Expressed Gene | Tcrb, T-cell receptor beta chain, mouse, laboratory | ||
| Expressed Gene | Tcra, T-cell receptor alpha chain, mouse, laboratory | ||
| General Note | Transgenic mice carry a T-cell receptor (Tcra-V2, Tcra-J TA31 / Tcrb-V8.1, Tcrb-D, Tcrb-J 2.4) specific for LCMV (lymphocytic choriomeningitis virus), H2-Db. | ||
| Molecular Note | The transgene carries both Tcra and Tcrb. Ten to twenty copies of the Tcr transgene were reported to have integrated on the same chromosome. At least two transgenic lines are known to exist. Line 327 is the representative line. The second line is 318. [MGI Ref ID J:77696] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(TcrLCMV)327Sdz, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Serreze DV; Johnson EA; Chapman HD; Graser RT; Marron MP; DiLorenzo TP; Silveira P; Yoshimura Y; Nathenson SG; Joyce S. 2001. Autoreactive diabetogenic T-cells in NOD mice can efficiently expand from a greatly reduced precursor pool. Diabetes 50(9):1992-2000. [PubMed: 11522664] [MGI Ref ID J:71050]
Additional References
Pircher H; Burki K; Lang R; Hengartner H; Zinkernagel RM. 1989. Tolerance induction in double specific T-cell receptor transgenic mice varies with antigen. Nature 342(6249):559-61. [PubMed: 2573841] [MGI Ref ID J:77696]
Tg(TcrLCMV)327Sdz relatedAichele P; Unsoeld H; Koschella M; Schweier O; Kalinke U; Vucikuja S. 2006. CD8 T cells specific for lymphocytic choriomeningitis virus require type I IFN receptor for clonal expansion. J Immunol 176(8):4525-9. [PubMed: 16585541] [MGI Ref ID J:131168]
Azzam HS; DeJarnette JB; Huang K; Emmons R; Park CS; Sommers CL; El-Khoury D; Shores EW; Love PE. 2001. Fine tuning of TCR signaling by CD5. J Immunol 166(9):5464-72. [PubMed: 11313384] [MGI Ref ID J:106735]
Bachmann MF; Waterhouse P; Speiser DE; McKall-Faienza K; Mak TW; Ohashi PS. 1998. Normal responsiveness of CTLA-4-deficient anti-viral cytotoxic T cells. J Immunol 160(1):95-100. [PubMed: 9551960] [MGI Ref ID J:111103]
Bahl K; Kim SK; Calcagno C; Ghersi D; Puzone R; Celada F; Selin LK; Welsh RM. 2006. IFN-induced attrition of CD8 T cells in the presence or absence of cognate antigen during the early stages of viral infections. J Immunol 176(7):4284-95. [PubMed: 16547266] [MGI Ref ID J:129873]
Beisner DR; Chu IH; Arechiga AF; Hedrick SM; Walsh CM. 2003. The requirements for Fas-associated death domain signaling in mature T cell activation and survival. J Immunol 171(1):247-56. [PubMed: 12817005] [MGI Ref ID J:132831]
Berg-Brown NN; Gronski MA; Jones RG; Elford AR; Deenick EK; Odermatt B; Littman DR; Ohashi PS. 2004. PKCtheta signals activation versus tolerance in vivo. J Exp Med 199(6):743-52. [PubMed: 15024044] [MGI Ref ID J:123989]
Biro J; Wurch A; Potocnik AJ; Falk I; Mossmann H; Eichmann K. 1999. Regulation of T cell receptor (TCR) beta gene expression by CD3 complex signaling in immature thymocytes: implications for TCRbeta allelic exclusion. Proc Natl Acad Sci U S A 96(7):3882-7. [PubMed: 10097132] [MGI Ref ID J:123031]
Blohm U; Potthoff D; van der Kogel AJ; Pircher H. 2006. Solid tumors 'melt' from the inside after successful CD8 T cell attack. Eur J Immunol 36(2):468-77. [PubMed: 16385625] [MGI Ref ID J:113858]
Bosco N; Agenes F; Ceredig R. 2005. Effects of increasing IL-7 availability on lymphocytes during and after lymphopenia-induced proliferation. J Immunol 175(1):162-70. [PubMed: 15972643] [MGI Ref ID J:100611]
Boyman O; Ramsey C; Kim DM; Sprent J; Surh CD. 2008. IL-7/anti-IL-7 mAb complexes restore T cell development and induce homeostatic T Cell expansion without lymphopenia. J Immunol 180(11):7265-75. [PubMed: 18490726] [MGI Ref ID J:136336]
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Brehm MA; Daniels KA; Welsh RM. 2005. Rapid production of TNF-alpha following TCR engagement of naive CD8 T cells. J Immunol 175(8):5043-9. [PubMed: 16210607] [MGI Ref ID J:119124]
Calzascia T; Pellegrini M; Lin A; Garza KM; Elford AR; Shahinian A; Ohashi PS; Mak TW. 2008. CD4 T cells, lymphopenia, and IL-7 in a multistep pathway to autoimmunity. Proc Natl Acad Sci U S A 105(8):2999-3004. [PubMed: 18287017] [MGI Ref ID J:132821]
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Christensen JE; Nansen A; Moos T; Lu B; Gerard C; Christensen JP; Thomsen AR. 2004. Efficient T-cell surveillance of the CNS requires expression of the CXC chemokine receptor 3. J Neurosci 24(20):4849-58. [PubMed: 15152045] [MGI Ref ID J:96905]
Crompton T; Moore M; MacDonald HR; Malissen B. 1994. Double-negative thymocyte subsets in CD3 zeta chain-deficient mice: absence of HSA+CD44-CD25- cells. Eur J Immunol 24(8):1903-7. [PubMed: 7520000] [MGI Ref ID J:112990]
Davidson D; Schraven B; Veillette A. 2007. PAG-associated FynT regulates calcium signaling and promotes anergy in T lymphocytes. Mol Cell Biol 27(5):1960-73. [PubMed: 17210649] [MGI Ref ID J:118860]
Ehl S; Hombach J; Aichele P; Hengartner H; Zinkernagel RM. 1997. Bystander activation of cytotoxic T cells: studies on the mechanism and evaluation of in vivo significance in a transgenic mouse model. J Exp Med 185(7):1241-51. [PubMed: 9104811] [MGI Ref ID J:107413]
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Gronski MA; Boulter JM; Moskophidis D; Nguyen LT; Holmberg K; Elford AR; Deenick EK; Kim HO; Penninger JM; Odermatt B; Gallimore A; Gascoigne NR; Ohashi PS. 2004. TCR affinity and negative regulation limit autoimmunity. Nat Med 10(11):1234-9. [PubMed: 15467726] [MGI Ref ID J:94025]
Gu X; Laouar A; Wan J; Daheshia M; Lieberman J; Yokoyama WM; Katz HR; Manjunath N. 2003. The gp49B1 inhibitory receptor regulates the IFN-gamma responses of T cells and NK cells. J Immunol 170(8):4095-101. [PubMed: 12682239] [MGI Ref ID J:134418]
Gu Y; Chae HD; Siefring JE; Jasti AC; Hildeman DA; Williams DA. 2006. RhoH GTPase recruits and activates Zap70 required for T cell receptor signaling and thymocyte development. Nat Immunol 7(11):1182-1190. [PubMed: 17028588] [MGI Ref ID J:113565]
Hermans IF; Ritchie DS; Daish A; Yang J; Kehry MR; Ronchese F. 1999. Impaired ability of MHC class II-/- dendritic cells to provide tumor protection is rescued by CD40 ligation. J Immunol 163(1):77-81. [PubMed: 10384102] [MGI Ref ID J:55653]
Iannacone M; Sitia G; Isogawa M; Whitmire JK; Marchese P; Chisari FV; Ruggeri ZM; Guidotti LG. 2008. Platelets prevent IFN-alpha/beta-induced lethal hemorrhage promoting CTL-dependent clearance of lymphocytic choriomeningitis virus. Proc Natl Acad Sci U S A 105(2):629-34. [PubMed: 18184798] [MGI Ref ID J:131089]
Intlekofer AM; Takemoto N; Kao C; Banerjee A; Schambach F; Northrop JK; Shen H; Wherry EJ; Reiner SL. 2007. Requirement for T-bet in the aberrant differentiation of unhelped memory CD8+ T cells. J Exp Med 204(9):2015-21. [PubMed: 17698591] [MGI Ref ID J:126087]
Intlekofer AM; Takemoto N; Wherry EJ; Longworth SA; Northrup JT; Palanivel VR; Mullen AC; Gasink CR; Kaech SM; Miller JD; Gapin L; Ryan K; Russ AP; Lindsten T; Orange JS; Goldrath AW; Ahmed R; Reiner SL. 2005. Effector and memory CD8+ T cell fate coupled by T-bet and eomesodermin. Nat Immunol 6(12):1236-44. [PubMed: 16273099] [MGI Ref ID J:112679]
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Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry This strain is maintained Tg/? x Tg/?.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00 Cryopreserved Embryos Fee $1600.00
Additional Supply Details
| Pricing for International shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00 Cryopreserved Embryos Fee $2080.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
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Fax: 207.288.6150
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. THE LABORATORY EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.
No Liability
In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.
MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.
Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.