Strain Name:

B6(Cg)-Ncf1m1J/J

Stock Number:

004742

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Peritoneal neutrophils and macrophages, bone marrow cells and neutrophils isolated from bone marrow of mice homozygous for Ncf1m1J spontaneous mutation fail to produce superoxide upon stimulation in vitro with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate 13-acetate (PMA).

Description

Strain Information

Former Names C57BL/6J-Ncf1m1J/J    (Changed: 08-DEC-05 )
Type Coisogenic; Mutant Strain; Spontaneous Mutation;
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Mating SystemHomozygote x Homozygote         (Female x Male)   13-SEP-13
Specieslaboratory mouse
GenerationN3+N2F3 (12-DEC-13)
Generation Definitions

Description
Peritoneal neutrophils and macrophages, bone marrow cells and neutrophils isolated from bone marrow of mice homozygous for Ncf1m1J fail to produce superoxide upon stimulation in vitro with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate 13-acetate (PMA), as determined by kinetic spectrophotometric analysis of cytochrome c reduction. Western blot analysis detected no full-length NCF1/p47phox protein in cells from these mice; a faint band of slightly smaller molecular size than the wild type NCF1 protein was observed on probing with antibodies to NCF1. To exclude the possibility that the NCF1 protein is produced in cells of mutant mice but is degraded rapidly by endogenous proteases, bone marrow cells were isolated and samples prepared for western blot analysis in the presence of diisopropyl fluorophosphate (DFP); no difference was observed upon analysis of freshly prepared cell lysates made with and without DFP, indicating that NCF1 protein is not expressed in mutant cells (Huang et al. 2000).

Analysis for other NADPH oxidases involved in neutrophil superoxide production revealed that NCF2/p67phox was present at wild type levels and CYBB/gp91phox and CYBA/p22phox were expressed at higher than wild type levels. Other neutrophil products wereassayed and found not to differ in bone marrow cells of C57BL/6J vs. mutant mice: GR-1, a granulocyte marker present on approximately 40% of marrow cells by fluorescence activated cell sorting (FACS) analysis; NCF4/p40phox and RAC1/RAC2 proteins, analyzed by western blot analysis; and expression of mRNAs encoding the granule proteins myeloperoxidase, elastase, cathepsin, lysozyme, lactoferrin and gelatinase as detected by reverse transcriptase - polymerase chain reaction (RT-PCR) (Huang et al. 2000).

The response of Ncf1m1J/Ncf1m1J mice to infectious or irritant (inflammatory stimulus) challenge has not been investigated (Huang et al. 2000). However, mice homozygous for the targeted mutation Ncf1tm1Shl - which disrupts the gene near the point of the Ncf1m1J mutation and, like the latter, results in failure of phagocytes to generate a superoxide burst following PMA stimulation in vitro - developed spontaneous infections with a variety of microorganisms including Staphylococcus xylosus, Lactobacillus, a hyaline septate mold and the fungus Paecilomyces, despite being maintained under specific pathogen free conditions. Ten-fold more Staphyloccoccus aureus bacteria survived intracellularly following in vitro uptake by phagocytes inblood from these mice than from control littermates, demonstrating a defect in the intracellular bactericidal activity of the mutant mice. Following intraperitoneal injection of the sterile irritant thioglycolate, twice as many leukocytes were recruited to the peritoneal cavities of Ncf1tm1Shl homozygous mice than of littermate controls; proportions of neutrophils in mutant and control exudates were similar (Jackson et al., J Exp Med 182:751-758 1995).

Development
This spontaneous mutation arose in the B6.BKS(D)-Leprdb/J (Stock No. 000697) production colony at The Jackson Laboratory. The mutation was identified in 1999. (Huang CK. et al. 2000) The mutations Leprdb and m have been selectively bred out of this strain by crossing to C57BL/6J at least three times.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Ncf1m1J allele
010606   NOD.Cg-Ncf1m1J/MxJ
View Strains carrying   Ncf1m1J     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-Positive, Type I
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Ncf1m1J/Ncf1m1J

        B6.Cg-Dock7m +/+ Leprdb/J
  • immune system phenotype
  • abnormal macrophage physiology
    • peritoneal macrophages fail to produce superoxide upon stimulation in vitro with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate 13-acetate (PMA)   (MGI Ref ID J:83428)
  • decreased susceptibility to viral infection
    • following exposure to inactivated H5N1 virus, acute-lung injury is attenuated compared to in wild-type mice   (MGI Ref ID J:145306)
  • impaired granulocyte bactericidal activity
    • peritoneal neutrophils, bone marrow cells and neutrophils isolated from bone marrow fail to produce superoxide upon stimulation in vitro with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate 13-acetate (PMA)   (MGI Ref ID J:83428)
  • hematopoietic system phenotype
  • abnormal macrophage physiology
    • peritoneal macrophages fail to produce superoxide upon stimulation in vitro with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate 13-acetate (PMA)   (MGI Ref ID J:83428)
  • impaired granulocyte bactericidal activity
    • peritoneal neutrophils, bone marrow cells and neutrophils isolated from bone marrow fail to produce superoxide upon stimulation in vitro with N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate 13-acetate (PMA)   (MGI Ref ID J:83428)

Ncf1m1J/Ncf1m1J

        involves: C57BL/6
  • cellular phenotype
  • oxidative stress
    • in the sinoatrial node of STZ-treated mice   (MGI Ref ID J:207291)
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Research Applications
This mouse can be used to support research in many areas including:

Ncf1m1J related

Hematological Research
Immunological Defects
Neutrophil Defects
      NADPH oxydase deficiency

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      Neutrophil Defects
Inflammation
      Neutrophil defects

Research Tools
Immunology, Inflammation and Autoimmunity Research
      neutrophil NADPH oxydase deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ncf1m1J
Allele Name mutation 1, Jackson
Allele Type Spontaneous
Common Name(s) Ncf1*; p47-; p47phox; p47phox-`;
Strain of OriginB6.Cg-Dock7 +/+ Lepr/J
Gene Symbol and Name Ncf1, neutrophil cytosolic factor 1
Chromosome 5
Gene Common Name(s) NADPH oxidase subunit (47kDa); NCF1A; NOXO2; Ncf-1; SH3PXD1A; meutrophil cytosolic factor 1; p47phox; p47phox;
Molecular Note The mutation is an A to C transversion at -2 position at the 5' end of exon 8 of the Ncf1 gene, resulting in aberrant splicing of the Ncf1 transcript. Immunoblotting detected no intact NCF1 protein in cells from these mice. [MGI Ref ID J:83428]

Genotyping

Genotyping Information

Genotyping Protocols

Ncf1m1J-Endpoint, End Point Analysis
Ncf1m1J, Pyrosequencing
Ncf1m1J, Restriction Enzyme Digest


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Huang CK; Zhan L; Hannigan MO; Ai Y; Leto TL. 2000. P47(phox)-deficient NADPH oxidase defect in neutrophils of diabetic mouse strains, C57BL/6J-m db/db and db/+. J Leukoc Biol 67(2):210-5. [PubMed: 10670582]  [MGI Ref ID J:83428]

Additional References

Hultqvist M; Olofsson P; Holmberg J; Backstrom BT; Tordsson J; Holmdahl R. 2004. Enhanced autoimmunity, arthritis, and encephalomyelitis in mice with a reduced oxidative burst due to a mutation in the Ncf1 gene. Proc Natl Acad Sci U S A 101(34):12646-51. [PubMed: 15310853]  [MGI Ref ID J:92437]

Ncf1m1J related

Aachoui Y; Leaf IA; Hagar JA; Fontana MF; Campos CG; Zak DE; Tan MH; Cotter PA; Vance RE; Aderem A; Miao EA. 2013. Caspase-11 protects against bacteria that escape the vacuole. Science 339(6122):975-8. [PubMed: 23348507]  [MGI Ref ID J:193387]

Aoki T; Nishimura M; Kataoka H; Ishibashi R; Nozaki K; Hashimoto N. 2009. Reactive oxygen species modulate growth of cerebral aneurysms: a study using the free radical scavenger edaravone and p47phox(-/-) mice. Lab Invest 89(7):730-41. [PubMed: 19381132]  [MGI Ref ID J:149896]

Barakat DJ; Dvoriantchikova G; Ivanov D; Shestopalov VI. 2012. Astroglial NF-kappaB mediates oxidative stress by regulation of NADPH oxidase in a model of retinal ischemia reperfusion injury. J Neurochem 120(4):586-97. [PubMed: 22118627]  [MGI Ref ID J:182276]

Batsalova T; Dzhambazov B; Klaczkowska D; Holmdahl R. 2010. Mice producing less reactive oxygen species are relatively resistant to collagen glycopeptide vaccination against arthritis. J Immunol 185(5):2701-9. [PubMed: 20686129]  [MGI Ref ID J:163242]

Bauernfeind F; Bartok E; Rieger A; Franchi L; Nunez G; Hornung V. 2011. Cutting edge: reactive oxygen species inhibitors block priming, but not activation, of the NLRP3 inflammasome. J Immunol 187(2):613-7. [PubMed: 21677136]  [MGI Ref ID J:178032]

Brennan AM; Suh SW; Won SJ; Narasimhan P; Kauppinen TM; Lee H; Edling Y; Chan PH; Swanson RA. 2009. NADPH oxidase is the primary source of superoxide induced by NMDA receptor activation. Nat Neurosci 12(7):857-63. [PubMed: 19503084]  [MGI Ref ID J:152577]

Campuzano V; Segura-Puimedon M; Terrado V; Sanchez-Rodriguez C; Coustets M; Menacho-Marquez M; Nevado J; Bustelo XR; Francke U; Perez-Jurado LA. 2012. Reduction of NADPH-oxidase activity ameliorates the cardiovascular phenotype in a mouse model of Williams-Beuren Syndrome. PLoS Genet 8(2):e1002458. [PubMed: 22319452]  [MGI Ref ID J:181479]

Carlsen ED; Hay C; Henard CA; Popov V; Garg NJ; Soong L. 2013. Leishmania amazonensis Amastigotes Trigger Neutrophil Activation but Resist Neutrophil Microbicidal Mechanisms. Infect Immun 81(11):3966-74. [PubMed: 23918780]  [MGI Ref ID J:201897]

Chiriac MT; Roesler J; Sindrilaru A; Scharffetter-Kochanek K; Zillikens D; Sitaru C. 2007. NADPH oxidase is required for neutrophil-dependent autoantibody-induced tissue damage. J Pathol 212(1):56-65. [PubMed: 17380558]  [MGI Ref ID J:122096]

Croker BA; Lewis RS; Babon JJ; Mintern JD; Jenne DE; Metcalf D; Zhang JG; Cengia LH; O'Donnell JA; Roberts AW. 2011. Neutrophils require SHP1 to regulate IL-1beta production and prevent inflammatory skin disease. J Immunol 186(2):1131-9. [PubMed: 21160041]  [MGI Ref ID J:168769]

D'Angelo C; De Luca A; Zelante T; Bonifazi P; Moretti S; Giovannini G; Iannitti RG; Zagarella S; Bozza S; Campo S; Salvatori G; Romani L. 2009. Exogenous pentraxin 3 restores antifungal resistance and restrains inflammation in murine chronic granulomatous disease. J Immunol 183(7):4609-18. [PubMed: 19734205]  [MGI Ref ID J:152794]

Deffert C; Carnesecchi S; Yuan H; Rougemont AL; Kelkka T; Holmdahl R; Krause KH; Schappi MG. 2012. Hyperinflammation of chronic granulomatous disease is abolished by NOX2 reconstitution in macrophages and dendritic cells. J Pathol 228(3):341-50. [PubMed: 22685019]  [MGI Ref ID J:188429]

Dietlin TA; Hofman FM; Lund BT; Gilmore W; Stohlman SA; van der Veen RC. 2007. Mycobacteria-induced Gr-1+ subsets from distinct myeloid lineages have opposite effects on T cell expansion. J Leukoc Biol 81(5):1205-12. [PubMed: 17307863]  [MGI Ref ID J:121711]

Doyle T; Esposito E; Bryant L; Cuzzocrea S; Salvemini D. 2013. NADPH-oxidase 2 activation promotes opioid-induced antinociceptive tolerance in mice. Neuroscience 241:1-9. [PubMed: 23454539]  [MGI Ref ID J:201366]

Efimova O; Szankasi P; Kelley TW. 2011. Ncf1 (p47phox) is essential for direct regulatory T cell mediated suppression of CD4+ effector T cells. PLoS One 6(1):e16013. [PubMed: 21253614]  [MGI Ref ID J:180830]

Erickson JR; Joiner ML; Guan X; Kutschke W; Yang J; Oddis CV; Bartlett RK; Lowe JS; O'Donnell SE; Aykin-Burns N; Zimmerman MC; Zimmerman K; Ham AJ; Weiss RM; Spitz DR; Shea MA; Colbran RJ; Mohler PJ; Anderson ME. 2008. A dynamic pathway for calcium-independent activation of CaMKII by methionine oxidation. Cell 133(3):462-74. [PubMed: 18455987]  [MGI Ref ID J:145301]

Goldmann O; von Kockritz-Blickwede M; Holtje C; Chhatwal GS; Geffers R; Medina E. 2007. Transcriptome analysis of murine macrophages in response to infection with Streptococcus pyogenes reveals an unusual activation program. Infect Immun 75(8):4148-57. [PubMed: 17526748]  [MGI Ref ID J:123374]

Grimm MJ; Vethanayagam RR; Almyroudis NG; Dennis CG; Khan AN; D'Auria AC; Singel KL; Davidson BA; Knight PR; Blackwell TS; Hohl TM; Mansour MK; Vyas JM; Rohm M; Urban CF; Kelkka T; Holmdahl R; Segal BH. 2013. Monocyte- and macrophage-targeted NADPH oxidase mediates antifungal host defense and regulation of acute inflammation in mice. J Immunol 190(8):4175-84. [PubMed: 23509361]  [MGI Ref ID J:195295]

Gupte SA; Kaminski PM; George S; Kouznestova L; Olson SC; Mathew R; Hintze TH; Wolin MS. 2009. Peroxide generation by p47phox-Src activation of Nox2 has a key role in protein kinase C-induced arterial smooth muscle contraction. Am J Physiol Heart Circ Physiol 296(4):H1048-57. [PubMed: 19168729]  [MGI Ref ID J:150905]

Hagenow K; Gelderman KA; Hultqvist M; Merky P; Backlund J; Frey O; Kamradt T; Holmdahl R. 2009. Ncf1-associated reduced oxidative burst promotes IL-33R+ T cell-mediated adjuvant-free arthritis in mice. J Immunol 183(2):874-81. [PubMed: 19553535]  [MGI Ref ID J:151401]

He BJ; Joiner ML; Singh MV; Luczak ED; Swaminathan PD; Koval OM; Kutschke W; Allamargot C; Yang J; Guan X; Zimmerman K; Grumbach IM; Weiss RM; Spitz DR; Sigmund CD; Blankesteijn WM; Heymans S; Mohler PJ; Anderson ME. 2011. Oxidation of CaMKII determines the cardiotoxic effects of aldosterone. Nat Med 17(12):1610-8. [PubMed: 22081025]  [MGI Ref ID J:180205]

Hultqvist M; Backlund J; Bauer K; Gelderman KA; Holmdahl R. 2007. Lack of reactive oxygen species breaks T cell tolerance to collagen type II and allows development of arthritis in mice. J Immunol 179(3):1431-7. [PubMed: 17641008]  [MGI Ref ID J:149954]

Hultqvist M; Olofsson P; Holmberg J; Backstrom BT; Tordsson J; Holmdahl R. 2004. Enhanced autoimmunity, arthritis, and encephalomyelitis in mice with a reduced oxidative burst due to a mutation in the Ncf1 gene. Proc Natl Acad Sci U S A 101(34):12646-51. [PubMed: 15310853]  [MGI Ref ID J:92437]

Imai Y; Kuba K; Neely GG; Yaghubian-Malhami R; Perkmann T; van Loo G; Ermolaeva M; Veldhuizen R; Leung YH; Wang H; Liu H; Sun Y; Pasparakis M; Kopf M; Mech C; Bavari S; Peiris JS; Slutsky AS; Akira S; Hultqvist M; Holmdahl R; Nicholls J; Jiang C; Binder CJ; Penninger JM. 2008. Identification of oxidative stress and Toll-like receptor 4 signaling as a key pathway of acute lung injury. Cell 133(2):235-49. [PubMed: 18423196]  [MGI Ref ID J:145306]

Jun J; Savransky V; Nanayakkara A; Bevans S; Li J; Smith PL; Polotsky VY. 2008. Intermittent hypoxia has organ-specific effects on oxidative stress. Am J Physiol Regul Integr Comp Physiol 295(4):R1274-81. [PubMed: 18703411]  [MGI Ref ID J:148563]

Kelkka T; Hultqvist M; Nandakumar KS; Holmdahl R. 2012. Enhancement of Antibody-Induced Arthritis via Toll-Like Receptor 2 Stimulation Is Regulated by Granulocyte Reactive Oxygen Species. Am J Pathol 181(1):141-50. [PubMed: 22642907]  [MGI Ref ID J:185524]

Liu G; Vogel SM; Gao X; Javaid K; Hu G; Danilov SM; Malik AB; Minshall RD. 2011. Src phosphorylation of endothelial cell surface intercellular adhesion molecule-1 mediates neutrophil adhesion and contributes to the mechanism of lung inflammation. Arterioscler Thromb Vasc Biol 31(6):1342-50. [PubMed: 21474822]  [MGI Ref ID J:191473]

Luo M; Guan X; Luczak ED; Lang D; Kutschke W; Gao Z; Yang J; Glynn P; Sossalla S; Swaminathan PD; Weiss RM; Yang B; Rokita AG; Maier LS; Efimov IR; Hund TJ; Anderson ME. 2013. Diabetes increases mortality after myocardial infarction by oxidizing CaMKII. J Clin Invest 123(3):1262-74. [PubMed: 23426181]  [MGI Ref ID J:207291]

Mao H; Kano G; Hudson SA; Brummet M; Zimmermann N; Zhu Z; Bochner BS. 2013. Mechanisms of Siglec-F-induced eosinophil apoptosis: a role for caspases but not for SHP-1, Src kinases, NADPH oxidase or reactive oxygen. PLoS One 8(6):e68143. [PubMed: 23840825]  [MGI Ref ID J:204321]

Miao EA; Leaf IA; Treuting PM; Mao DP; Dors M; Sarkar A; Warren SE; Wewers MD; Aderem A. 2010. Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria. Nat Immunol 11(12):1136-42. [PubMed: 21057511]  [MGI Ref ID J:167323]

Owens AP 3rd; Subramanian V; Moorleghen JJ; Guo Z; McNamara CA; Cassis LA; Daugherty A. 2010. Angiotensin II induces a region-specific hyperplasia of the ascending aorta through regulation of inhibitor of differentiation 3. Circ Res 106(3):611-9. [PubMed: 20019328]  [MGI Ref ID J:170884]

Pizzolla A; Hultqvist M; Nilson B; Grimm MJ; Eneljung T; Jonsson IM; Verdrengh M; Kelkka T; Gjertsson I; Segal BH; Holmdahl R. 2012. Reactive oxygen species produced by the NADPH oxidase 2 complex in monocytes protect mice from bacterial infections. J Immunol 188(10):5003-11. [PubMed: 22491245]  [MGI Ref ID J:188685]

Pizzolla A; Wing K; Holmdahl R. 2013. A Glucose-6-Phosphate Isomerase Peptide Induces T and B Cell-Dependent Chronic Arthritis in C57BL/10 Mice: Arthritis without Reactive Oxygen Species and Complement. Am J Pathol 183(4):1144-55. [PubMed: 23911657]  [MGI Ref ID J:200919]

Purushothaman D; Sarin A. 2009. Cytokine-dependent regulation of NADPH oxidase activity and the consequences for activated T cell homeostasis. J Exp Med 206(7):1515-23. [PubMed: 19546249]  [MGI Ref ID J:150270]

Reyes RC; Brennan AM; Shen Y; Baldwin Y; Swanson RA. 2012. Activation of Neuronal NMDA Receptors Induces Superoxide-Mediated Oxidative Stress in Neighboring Neurons and Astrocytes. J Neurosci 32(37):12973-8. [PubMed: 22973021]  [MGI Ref ID J:188112]

Ronis MJ; Sharma N; Vantrease J; Borengasser SJ; Ferguson M; Mercer KE; Cleves MA; Gomez-Acevedo H; Badger TM. 2013. Female mice lacking p47phox have altered adipose tissue gene expression and are protected against high fat-induced obesity. Physiol Genomics 45(9):351-66. [PubMed: 23482812]  [MGI Ref ID J:197531]

Sareila O; Jaakkola N; Olofsson P; Kelkka T; Holmdahl R. 2013. Identification of a region in p47phox/NCF1 crucial for phagocytic NADPH oxidase (NOX2) activation. J Leukoc Biol 93(3):427-35. [PubMed: 23271700]  [MGI Ref ID J:198082]

Seleme MC; Lei W; Burg AR; Goh KY; Metz A; Steele C; Tse HM. 2012. Dysregulated TLR3-dependent signaling and innate immune activation in superoxide-deficient macrophages from nonobese diabetic mice. Free Radic Biol Med 52(9):2047-56. [PubMed: 22361747]  [MGI Ref ID J:183254]

Semprun-Prieto LC; Sukhanov S; Yoshida T; Rezk BM; Gonzalez-Villalobos RA; Vaughn C; Michael Tabony A; Delafontaine P. 2011. Angiotensin II induced catabolic effect and muscle atrophy are redox dependent. Biochem Biophys Res Commun 409(2):217-21. [PubMed: 21570954]  [MGI Ref ID J:172592]

Seo JS; Park JY; Choi J; Kim TK; Shin JH; Lee JK; Han PL. 2012. NADPH oxidase mediates depressive behavior induced by chronic stress in mice. J Neurosci 32(28):9690-9. [PubMed: 22787054]  [MGI Ref ID J:186471]

Shakya AK; Kumar A; Klaczkowska D; Hultqvist M; Hagenow K; Holmdahl R; Nandakumar KS. 2011. Collagen Type II and a Thermo-Responsive Polymer of N-Isopropylacrylamide Induce Arthritis Independent of Toll-Like Receptors A Strong Influence by Major Histocompatibility Complex Class II and Ncf1 Genes. Am J Pathol 179(5):2490-500. [PubMed: 21933654]  [MGI Ref ID J:177359]

Spencer NY; Yan Z; Boudreau RL; Zhang Y; Luo M; Li Q; Tian X; Shah AM; Davisson RL; Davidson B; Banfi B; Engelhardt JF. 2011. Control of hepatic nuclear superoxide production by glucose 6-phosphate dehydrogenase and NADPH oxidase-4. J Biol Chem 286(11):8977-87. [PubMed: 21212270]  [MGI Ref ID J:170529]

Suh SW; Gum ET; Hamby AM; Chan PH; Swanson RA. 2007. Hypoglycemic neuronal death is triggered by glucose reperfusion and activation of neuronal NADPH oxidase. J Clin Invest 117(4):910-8. [PubMed: 17404617]  [MGI Ref ID J:121255]

Tadie JM; Bae HB; Banerjee S; Zmijewski JW; Abraham E. 2012. Differential activation of RAGE by HMGB1 modulates neutrophil-associated NADPH oxidase activity and bacterial killing. Am J Physiol Cell Physiol 302(1):C249-56. [PubMed: 22012330]  [MGI Ref ID J:180623]

Thayer TC; Delano M; Liu C; Chen J; Padgett LE; Tse HM; Annamali M; Piganelli JD; Moldawer LL; Mathews CE. 2011. Superoxide production by macrophages and T cells is critical for the induction of autoreactivity and type 1 diabetes. Diabetes 60(8):2144-51. [PubMed: 21715554]  [MGI Ref ID J:186804]

Tse HM; Thayer TC; Steele C; Cuda CM; Morel L; Piganelli JD; Mathews CE. 2010. NADPH oxidase deficiency regulates Th lineage commitment and modulates autoimmunity. J Immunol 185(9):5247-58. [PubMed: 20881184]  [MGI Ref ID J:165189]

Walton JC; Selvakumar B; Weil ZM; Snyder SH; Nelson RJ. 2013. Neuronal nitric oxide synthase and NADPH oxidase interact to affect cognitive, affective, and social behaviors in mice. Behav Brain Res 256:320-7. [PubMed: 23948215]  [MGI Ref ID J:202345]

Wheeler ML; Defranco AL. 2012. Prolonged production of reactive oxygen species in response to B cell receptor stimulation promotes B cell activation and proliferation. J Immunol 189(9):4405-16. [PubMed: 23024271]  [MGI Ref ID J:190609]

Xu X; Yavar Z; Verdin M; Ying Z; Mihai G; Kampfrath T; Wang A; Zhong M; Lippmann M; Chen LC; Rajagopalan S; Sun Q. 2010. Effect of early particulate air pollution exposure on obesity in mice: role of p47phox. Arterioscler Thromb Vasc Biol 30(12):2518-27. [PubMed: 20864666]  [MGI Ref ID J:183206]

Yang Z; Sharma AK; Marshall M; Kron IL; Laubach VE. 2009. NADPH oxidase in bone marrow-derived cells mediates pulmonary ischemia-reperfusion injury. Am J Respir Cell Mol Biol 40(3):375-81. [PubMed: 18787174]  [MGI Ref ID J:157427]

Yao H; Edirisinghe I; Yang SR; Rajendrasozhan S; Kode A; Caito S; Adenuga D; Rahman I. 2008. Genetic ablation of NADPH oxidase enhances susceptibility to cigarette smoke-induced lung inflammation and emphysema in mice. Am J Pathol 172(5):1222-37. [PubMed: 18403597]  [MGI Ref ID J:134306]

Youn JY; Gao L; Cai H. 2012. The p47phox- and NADPH oxidase organiser 1 (NOXO1)-dependent activation of NADPH oxidase 1 (NOX1) mediates endothelial nitric oxide synthase (eNOS) uncoupling and endothelial dysfunction in a streptozotocin-induced murine model of diabetes. Diabetologia 55(7):2069-79. [PubMed: 22549734]  [MGI Ref ID J:186449]

Yu G; Bolon M; Laird DW; Tyml K. 2010. Hypoxia and reoxygenation-induced oxidant production increase in microvascular endothelial cells depends on connexin40. Free Radic Biol Med 49(6):1008-13. [PubMed: 20541007]  [MGI Ref ID J:164462]

Zhang A; Jia Z; Wang N; Tidwell TJ; Yang T. 2011. Relative contributions of mitochondria and NADPH oxidase to deoxycorticosterone acetate-salt hypertension in mice. Kidney Int 80(1):51-60. [PubMed: 21368743]  [MGI Ref ID J:194724]

Zhang W; Wang T; Pei Z; Miller DS; Wu X; Block ML; Wilson B; Zhang W; Zhou Y; Hong JS; Zhang J. 2005. Aggregated alpha-synuclein activates microglia: a process leading to disease progression in Parkinson's disease. FASEB J 19(6):533-42. [PubMed: 15791003]  [MGI Ref ID J:105146]

Zhang WJ; Wei H; Frei B. 2009. Genetic deficiency of NADPH oxidase does not diminish, but rather enhances, LPS-induced acute inflammatory responses in vivo. Free Radic Biol Med 46(6):791-8. [PubMed: 19124074]  [MGI Ref ID J:145980]

Zhang WJ; Wei H; Tien YT; Frei B. 2011. Genetic ablation of phagocytic NADPH oxidase in mice limits TNFalpha-induced inflammation in the lungs but not other tissues. Free Radic Biol Med 50(11):1517-25. [PubMed: 21376114]  [MGI Ref ID J:172087]

Zmijewski JW; Lorne E; Banerjee S; Abraham E. 2009. Participation of mitochondrial respiratory complex III in neutrophil activation and lung injury. Am J Physiol Lung Cell Mol Physiol 296(4):L624-34. [PubMed: 19168575]  [MGI Ref ID J:149706]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Mating SystemHomozygote x Homozygote         (Female x Male)   13-SEP-13
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $195.00Female or MaleHomozygous for Ncf1m1J  
Price per Pair (US dollars $)Pair Genotype
$390.00Homozygous for Ncf1m1J x Homozygous for Ncf1m1J  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $253.50Female or MaleHomozygous for Ncf1m1J  
Price per Pair (US dollars $)Pair Genotype
$507.00Homozygous for Ncf1m1J x Homozygous for Ncf1m1J  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

General Supply Notes

  • View the complete collection of spontaneous mutants in the Mouse Mutant Resource.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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