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| These transgenic mice express Cre recombinase under the control of the mouse transgelin (smooth muscle protein 22-alpha) promoter. Cre recombinase activity is observed in vascular smooth muscle cells of hepatic and pulmonary arteries. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in vascular smooth muscle cells. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful in studies of vascular diseases such as atherosclerosis. | |||||||||||||||||||
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Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation F?+F15 (24-MAR-11)
Generation DefinitionsDonating Investigator Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas Description
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse transgelin (smooth muscle protein 22-alpha) promoter. Cre recombinase expression (mRNA) closely patterns endogenous transgelin expression, with the highest levels detected in the aorta, intestine and uterus. Low levels of transcript are detected in all other organs tested, likely reflecting the vascular smooth muscle compartments in the these tissues. Cre recombinase activity is observed in vascular smooth muscle cells of hepatic and pulmonary arteries, with no activity detected outside the vascular walls. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in vascular smooth muscle cells. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful in studies of vascular diseases such as atherosclerosis.Development
A transgenic construct containing cre coding sequence under the control of the mouse transgelin promoter was introduced into B6SJLF2 donor oocytes.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(Tagln-cre)1Her allele
017491 B6.Cg-Tg(Tagln-cre)1Her/J View Strains carrying Tg(Tagln-cre)1Her (1 strain)
006878 B6.129S6-Taglntm2(cre)Yec/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 017602 C57BL/6-Tg(Tagln-Tfpi)1661Rdsi/J 006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Tagln-cre)1Her | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Joachim Herz | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | SM; SM22-Cre; SM22-Cretg; SM22a-Cre; Sm22alphaCre; Tag1-Cre; Tgln-Cre; | ||
| Mutation Made By | Dr. Joachim Herz, Univ of Texas Southwest Med Ctr Dallas | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | vascular smooth muscle cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Tagln, transgelin, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Tagln-cre)1Her, transgene insertion 1, Joachim Herz | ||
| Chromosome | UN | ||
| Gene Common Name(s) | SM22-Cre; SM22-Cretg; Sm22alphaCre; Tag1-Cre; | ||
| Driver Note | Tagln | ||
| General Note | Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. | ||
| Molecular Note | The transgene consists of a Tagln (transgelin) promoter and a cre gene modified to include translational consensus and nuclear localization signals. The Tagln promoter was used to drive transgene expression in smooth muscle cells. Cre recombinase mRNA expression closely patterns endogenous transgelin expression, with the highest levels detected in the aorta, intestine, and uterus. Low levels of Cre recombinase transcript are detected in all other organs. Recombination occurs in vascular smooth muscle cells of hepatic and pulmonary arteries. No recombination has been detected in non-muscle cells. [MGI Ref ID J:76460] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Quantitative PCR, QPCR
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Boucher P; Gotthardt M; Li WP; Anderson RG; Herz J. 2003. LRP: role in vascular wall integrity and protection from atherosclerosis. Science 300(5617):329-32. [PubMed: 12690199] [MGI Ref ID J:82871]
Additional References
Holtwick R; Gotthardt M; Skryabin B; Steinmetz M; Potthast R; Zetsche B; Hammer RE; Herz J; Kuhn M. 2002. Smooth muscle-selective deletion of guanylyl cyclase-A prevents the acute but not chronic effects of ANP on blood pressure. Proc Natl Acad Sci U S A 99(10):7142-7. [PubMed: 11997476] [MGI Ref ID J:76460]
Tg(Tagln-cre)1Her relatedAlbinsson S; Suarez Y; Skoura A; Offermanns S; Miano JM; Sessa WC. 2010. MicroRNAs are necessary for vascular smooth muscle growth, differentiation, and function. Arterioscler Thromb Vasc Biol 30(6):1118-26. [PubMed: 20378849] [MGI Ref ID J:176638]
Arboleda-Velasquez JF; Manent J; Lee JH; Tikka S; Ospina C; Vanderburg CR; Frosch MP; Rodriguez-Falcon M; Villen J; Gygi S; Lopera F; Kalimo H; Moskowitz MA; Ayata C; Louvi A; Artavanis-Tsakonas S. 2011. PNAS Plus: Hypomorphic Notch 3 alleles link Notch signaling to ischemic cerebral small-vessel disease. Proc Natl Acad Sci U S A 108(21):E128-35. [PubMed: 21555590] [MGI Ref ID J:171887]
Arboleda-Velasquez JF; Zhou Z; Shin HK; Louvi A; Kim HH; Savitz SI; Liao JK; Salomone S; Ayata C; Moskowitz MA; Artavanis-Tsakonas S. 2008. Linking Notch signaling to ischemic stroke. Proc Natl Acad Sci U S A 105(12):4856-61. [PubMed: 18347334] [MGI Ref ID J:133358]
Basford JE; Moore ZW; Zhou L; Herz J; Hui DY. 2009. Smooth muscle LDL receptor-related protein-1 inactivation reduces vascular reactivity and promotes injury-induced neointima formation. Arterioscler Thromb Vasc Biol 29(11):1772-8. [PubMed: 19729608] [MGI Ref ID J:167799]
Boucher P; Li WP; Matz RL; Takayama Y; Auwerx J; Anderson RG; Herz J. 2007. LRP1 functions as an atheroprotective integrator of TGFbeta and PDFG signals in the vascular wall: implications for Marfan syndrome. PLoS ONE 2(5):e448. [PubMed: 17505534] [MGI Ref ID J:129347]
Carvalho RL; Itoh F; Goumans MJ; Lebrin F; Kato M; Takahashi S; Ema M; Itoh S; van Rooijen M; Bertolino P; Ten Dijke P; Mummery CL. 2007. Compensatory signalling induced in the yolk sac vasculature by deletion of TGF receptors in mice. J Cell Sci 120(Pt 24):4269-77. [PubMed: 18029401] [MGI Ref ID J:128498]
Chen Z; Wu J; Yang C; Fan P; Balazs L; Jiao Y; Lu M; Gu W; Li C; Pfeffer LM; Tigyi G; Yue J. 2012. DiGeorge syndrome critical region 8 (DGCR8) protein-mediated microRNA biogenesis is essential for vascular smooth muscle cell development in mice. J Biol Chem 287(23):19018-28. [PubMed: 22511778] [MGI Ref ID J:186203]
Cyphert JM; Allen IC; Church RJ; Latour AM; Snouwaert JN; Coffman TM; Koller BH. 2012. Allergic inflammation induces a persistent mechanistic switch in thromboxane-mediated airway constriction in the mouse. Am J Physiol Lung Cell Mol Physiol 302(1):L140-51. [PubMed: 21984570] [MGI Ref ID J:183333]
Ding M; Carrao AC; Wagner RJ; Xie Y; Jin Y; Rzucidlo EM; Yu J; Li W; Tellides G; Hwa J; Aprahamian TR; Martin KA. 2012. Vascular smooth muscle cell-derived adiponectin: a paracrine regulator of contractile phenotype. J Mol Cell Cardiol 52(2):474-84. [PubMed: 21952104] [MGI Ref ID J:183565]
El-Bizri N; Guignabert C; Wang L; Cheng A; Stankunas K; Chang CP; Mishina Y; Rabinovitch M. 2008. SM22alpha-targeted deletion of bone morphogenetic protein receptor 1A in mice impairs cardiac and vascular development, and influences organogenesis. Development 135(17):2981-91. [PubMed: 18667463] [MGI Ref ID J:139945]
Feng X; Krebs LT; Gridley T. 2010. Patent ductus arteriosus in mice with smooth muscle-specific Jag1 deletion. Development 137(24):4191-9. [PubMed: 21068062] [MGI Ref ID J:166769]
Fox EA; Biddinger JE; Jones KR; McAdams J; Worman A. 2013. Mechanism of hyperphagia contributing to obesity in brain-derived neurotrophic factor knockout mice. Neuroscience 229:176-99. [PubMed: 23069761] [MGI Ref ID J:193539]
French WJ; Creemers EE; Tallquist MD. 2008. Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol 28(18):5646-57. [PubMed: 18606782] [MGI Ref ID J:140245]
Frutkin AD; Shi H; Otsuka G; Leveen P; Karlsson S; Dichek DA. 2006. A critical developmental role for tgfbr2 in myogenic cell lineages is revealed in mice expressing SM22-Cre, not SMMHC-Cre. J Mol Cell Cardiol 41(4):724-31. [PubMed: 16887142] [MGI Ref ID J:114624]
Genetic Resource Sciences at The Jackson Laboratory. 2013. Expression/Specificity Patterns of Cre Alleles, 2013 MGI Direct Data Submission :. [MGI Ref ID J:193672]
Gomez-Arroyo J; Saleem SJ; Mizuno S; Syed AA; Bogaard HJ; Abbate A; Taraseviciene-Stewart L; Sung Y; Kraskauskas D; Farkas D; Conrad DH; Nicolls MR; Voelkel NF. 2012. A brief overview of mouse models of pulmonary arterial hypertension: problems and prospects. Am J Physiol Lung Cell Mol Physiol 302(10):L977-91. [PubMed: 22307907] [MGI Ref ID J:190182]
Greif DM; Kumar M; Lighthouse JK; Hum J; An A; Ding L; Red-Horse K; Espinoza FH; Olson L; Offermanns S; Krasnow MA. 2012. Radial construction of an arterial wall. Dev Cell 23(3):482-93. [PubMed: 22975322] [MGI Ref ID J:189012]
Hang CT; Yang J; Han P; Cheng HL; Shang C; Ashley E; Zhou B; Chang CP. 2010. Chromatin regulation by Brg1 underlies heart muscle development and disease. Nature 466(7302):62-7. [PubMed: 20596014] [MGI Ref ID J:162637]
Hansmann G; de Jesus Perez VA; Alastalo TP; Alvira CM; Guignabert C; Bekker JM; Schellong S; Urashima T; Wang L; Morrell NW; Rabinovitch M. 2008. An antiproliferative BMP-2/PPARgamma/apoE axis in human and murine SMCs and its role in pulmonary hypertension. J Clin Invest 118(5):1846-57. [PubMed: 18382765] [MGI Ref ID J:136168]
Hassane S; Claij N; Jodar M; Dedman A; Lauritzen I; Duprat F; Koenderman JS; van der Wal A; Breuning MH; de Heer E; Honore E; Deruiter MC; Peters DJ. 2011. Pkd1-inactivation in vascular smooth muscle cells and adaptation to hypertension. Lab Invest 91(1):24-32. [PubMed: 20856231] [MGI Ref ID J:167185]
Hernando E; Charytonowicz E; Dudas ME; Menendez S; Matushansky I; Mills J; Socci ND; Behrendt N; Ma L; Maki RG; Pandolfi PP; Cordon-Cardo C. 2007. The AKT-mTOR pathway plays a critical role in the development of leiomyosarcomas. Nat Med 13(6):748-753. [PubMed: 17496901] [MGI Ref ID J:121901]
Hofmann JJ; Zovein AC; Koh H; Radtke F; Weinmaster G; Iruela-Arispe ML. 2010. Jagged1 in the portal vein mesenchyme regulates intrahepatic bile duct development: insights into Alagille syndrome. Development 137(23):4061-72. [PubMed: 21062863] [MGI Ref ID J:166890]
Holtwick R; Gotthardt M; Skryabin B; Steinmetz M; Potthast R; Zetsche B; Hammer RE; Herz J; Kuhn M. 2002. Smooth muscle-selective deletion of guanylyl cyclase-A prevents the acute but not chronic effects of ANP on blood pressure. Proc Natl Acad Sci U S A 99(10):7142-7. [PubMed: 11997476] [MGI Ref ID J:76460]
Horiguchi M; Inoue T; Ohbayashi T; Hirai M; Noda K; Marmorstein LY; Yabe D; Takagi K; Akama TO; Kita T; Kimura T; Nakamura T. 2009. Fibulin-4 conducts proper elastogenesis via interaction with cross-linking enzyme lysyl oxidase. Proc Natl Acad Sci U S A 106(45):19029-34. [PubMed: 19855011] [MGI Ref ID J:154659]
Huang J; Davis EC; Chapman SL; Budatha M; Marmorstein LY; Word RA; Yanagisawa H. 2010. Fibulin-4 deficiency results in ascending aortic aneurysms: a potential link between abnormal smooth muscle cell phenotype and aneurysm progression. Circ Res 106(3):583-92. [PubMed: 20019329] [MGI Ref ID J:170883]
Huang Z; Rivas B; Agoulnik AI. 2012. Insulin-like 3 signaling is important for testicular descent but dispensable for spermatogenesis and germ cell survival in adult mice. Biol Reprod 87(6):143. [PubMed: 23100620] [MGI Ref ID J:194006]
Jaffe M; Sesti C; Washington IM; Du L; Dronadula N; Chin MT; Stolz DB; Davis EC; Dichek DA. 2012. Transforming Growth Factor-beta Signaling in Myogenic Cells Regulates Vascular Morphogenesis, Differentiation, and Matrix Synthesis. Arterioscler Thromb Vasc Biol 32(1):e1-e11. [PubMed: 21979435] [MGI Ref ID J:179089]
Kanekiyo T; Liu CC; Shinohara M; Li J; Bu G. 2012. LRP1 in brain vascular smooth muscle cells mediates local clearance of Alzheimer's amyloid-beta. J Neurosci 32(46):16458-65. [PubMed: 23152628] [MGI Ref ID J:192453]
Kuhn M; Volker K; Schwarz K; Carbajo-Lozoya J; Flogel U; Jacoby C; Stypmann J; van Eickels M; Gambaryan S; Hartmann M; Werner M; Wieland T; Schrader J; Baba HA. 2009. The natriuretic peptide/guanylyl cyclase--a system functions as a stress-responsive regulator of angiogenesis in mice. J Clin Invest 119(7):2019-30. [PubMed: 19487812] [MGI Ref ID J:152580]
Lasater EA; Li F; Bessler WK; Estes ML; Vemula S; Hingtgen CM; Dinauer MC; Kapur R; Conway SJ; Ingram DA Jr. 2010. Genetic and cellular evidence of vascular inflammation in neurofibromin-deficient mice and humans. J Clin Invest 120(3):859-70. [PubMed: 20160346] [MGI Ref ID J:158570]
Li L; Lai EY; Huang Y; Eisner C; Mizel D; Wilcox CS; Schnermann J. 2012. Renal afferent arteriolar and tubuloglomerular feedback reactivity in mice with conditional deletions of adenosine 1 receptors. Am J Physiol Renal Physiol 303(8):F1166-75. [PubMed: 22896040] [MGI Ref ID J:189800]
Lin CY; Lin CJ; Chen CH; Chen RM; Zhou B; Chang CP. 2012. The secondary heart field is a new site of calcineurin/Nfatc1 signaling for semilunar valve development. J Mol Cell Cardiol 52(5):1096-102. [PubMed: 22300732] [MGI Ref ID J:183686]
Long JB; Jay SM; Segal SS; Madri JA. 2009. VEGF-A and Semaphorin3A: modulators of vascular sympathetic innervation. Dev Biol 334(1):119-32. [PubMed: 19631637] [MGI Ref ID J:153552]
Lopes M; Gooupille O; Saint Cloment C; Robert B. 2012. Msx1 is expressed in retina endothelial cells at artery branching sites Biol Open :376-384. [MGI Ref ID J:184582]
Lukowski R; Weinmeister P; Bernhard D; Feil S; Gotthardt M; Herz J; Massberg S; Zernecke A; Weber C; Hofmann F; Feil R. 2008. Role of smooth muscle cGMP/cGKI signaling in murine vascular restenosis. Arterioscler Thromb Vasc Biol 28(7):1244-50. [PubMed: 18420996] [MGI Ref ID J:149021]
Malhowski AJ; Hira H; Bashiruddin S; Warburton R; Goto J; Robert B; Kwiatkowski DJ; Finlay GA. 2011. Smooth muscle protein-22-mediated deletion of Tsc1 results in cardiac hypertrophy that is mTORC1-mediated and reversed by rapamycin. Hum Mol Genet 20(7):1290-305. [PubMed: 21212099] [MGI Ref ID J:169239]
Nanba D; Kinugasa Y; Morimoto C; Koizumi M; Yamamura H; Takahashi K; Takakura N; Mekada E; Hashimoto K; Higashiyama S. 2006. Loss of HB-EGF in smooth muscle or endothelial cell lineages causes heart malformation. Biochem Biophys Res Commun 350(2):315-21. [PubMed: 17010937] [MGI Ref ID J:116051]
Navankasattusas S; Whitehead KJ; Suli A; Sorensen LK; Lim AH; Zhao J; Park KW; Wythe JD; Thomas KR; Chien CB; Li DY. 2008. The netrin receptor UNC5B promotes angiogenesis in specific vascular beds. Development 135(4):659-67. [PubMed: 18223200] [MGI Ref ID J:131817]
Olson LE; Soriano P. 2011. PDGFRbeta Signaling Regulates Mural Cell Plasticity and Inhibits Fat Development. Dev Cell 20(6):815-26. [PubMed: 21664579] [MGI Ref ID J:173602]
Otsuka G; Stempien-Otero A; Frutkin AD; Dichek DA. 2007. Mechanisms of TGF-beta1-induced intimal growth: plasminogen-independent activities of plasminogen activator inhibitor-1 and heterogeneous origin of intimal cells. Circ Res 100(9):1300-7. [PubMed: 17431190] [MGI Ref ID J:137777]
Pan Y; Balazs L; Tigyi G; Yue J. 2011. Conditional deletion of Dicer in vascular smooth muscle cells leads to the developmental delay and embryonic mortality. Biochem Biophys Res Commun 408(3):369-74. [PubMed: 21371421] [MGI Ref ID J:172088]
Rateri DL; Moorleghen JJ; Balakrishnan A; Owens AP 3rd; Howatt DA; Subramanian V; Poduri A; Charnigo R; Cassis LA; Daugherty A. 2011. Endothelial Cell-Specific Deficiency of Ang II Type 1a Receptors Attenuates Ang II-Induced Ascending Aortic Aneurysms in LDL Receptor-/- Mice. Circ Res 108(5):574-81. [PubMed: 21252156] [MGI Ref ID J:170230]
Rindler TN; Dostanic I; Lasko VM; Nieman ML; Neumann JC; Lorenz JN; Lingrel JB. 2011. Knockout of the Na,K-ATPase {alpha}2-isoform in the cardiovascular system does not alter basal blood pressure but prevents ACTH-induced hypertension. Am J Physiol Heart Circ Physiol 301(4):H1396-404. [PubMed: 21856916] [MGI Ref ID J:177051]
Sabrane K; Gambaryan S; Brandes RP; Holtwick R; Voss M; Kuhn M. 2003. Increased sensitivity to endothelial nitric oxide (NO) contributes to arterial normotension in mice with vascular smooth muscle-selective deletion of the atrial natriuretic peptide (ANP) receptor. J Biol Chem 278(20):17963-8. [PubMed: 12637561] [MGI Ref ID J:124744]
Schreier B; Dohler M; Rabe S; Schneider B; Schwerdt G; Ruhs S; Sibilia M; Gotthardt M; Gekle M; Grossmann C. 2011. Consequences of epidermal growth factor receptor (ErbB1) loss for vascular smooth muscle cells from mice with targeted deletion of ErbB1. Arterioscler Thromb Vasc Biol 31(7):1643-52. [PubMed: 21512163] [MGI Ref ID J:191470]
Sharif-Naeini R; Folgering JH; Bichet D; Duprat F; Lauritzen I; Arhatte M; Jodar M; Dedman A; Chatelain FC; Schulte U; Retailleau K; Loufrani L; Patel A; Sachs F; Delmas P; Peters DJ; Honore E. 2009. Polycystin-1 and -2 dosage regulates pressure sensing. Cell 139(3):587-96. [PubMed: 19879844] [MGI Ref ID J:157302]
Shen Z; Li C; Frieler RA; Gerasimova AS; Lee SJ; Wu J; Wang MM; Lumeng CN; Brosius FC 3rd; Duan SZ; Mortensen RM. 2012. Smooth muscle protein 22 alpha-Cre is expressed in myeloid cells in mice. Biochem Biophys Res Commun 422(4):639-42. [PubMed: 22609406] [MGI Ref ID J:184892]
Stankunas K; Hang CT; Tsun ZY; Chen H; Lee NV; Wu JI; Shang C; Bayle JH; Shou W; Iruela-Arispe ML; Chang CP. 2008. Endocardial Brg1 represses ADAMTS1 to maintain the microenvironment for myocardial morphogenesis. Dev Cell 14(2):298-311. [PubMed: 18267097] [MGI Ref ID J:132583]
Steinmetz M; Potthast R; Sabrane K; Kuhn M. 2004. Diverging vasorelaxing effects of C-type natriuretic peptide in renal resistance arteries and aortas of GC-A-deficient mice. Regul Pept 119(1-2):31-7. [PubMed: 15093694] [MGI Ref ID J:102436]
Subramanian V; Golledge J; Ijaz T; Bruemmer D; Daugherty A. 2010. Pioglitazone-induced reductions in atherosclerosis occur via smooth muscle cell-specific interaction with PPAR{gamma}. Circ Res 107(8):953-8. [PubMed: 20798360] [MGI Ref ID J:178193]
Tang W; Zeve D; Suh JM; Bosnakovski D; Kyba M; Hammer RE; Tallquist MD; Graff JM. 2008. White fat progenitor cells reside in the adipose vasculature. Science 322(5901):583-6. [PubMed: 18801968] [MGI Ref ID J:178521]
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Turlo KA; Noel OD; Vora R; LaRussa M; Fassler R; Hall-Glenn F; Iruela-Arispe ML. 2012. An essential requirement for beta1 integrin in the assembly of extracellular matrix proteins within the vascular wall. Dev Biol 365(1):23-35. [PubMed: 22331032] [MGI Ref ID J:184940]
Umans L; Cox L; Tjwa M; Bito V; Vermeire L; Laperre K; Sipido K; Moons L; Huylebroeck D; Zwijsen A. 2007. Inactivation of Smad5 in Endothelial Cells and Smooth Muscle Cells Demonstrates that Smad5 Is Required for Cardiac Homeostasis. Am J Pathol 170(5):1460-72. [PubMed: 17456754] [MGI Ref ID J:121072]
Varadkar P; Kraman M; Despres D; Ma G; Lozier J; McCright B. 2008. Notch2 is required for the proliferation of cardiac neural crest-derived smooth muscle cells. Dev Dyn 237(4):1144-52. [PubMed: 18330927] [MGI Ref ID J:132939]
Viana R; Batourina E; Huang H; Dressler GR; Kobayashi A; Behringer RR; Shapiro E; Hensle T; Lambert S; Mendelsohn C. 2007. The development of the bladder trigone, the center of the anti-reflux mechanism. Development 134(20):3763-9. [PubMed: 17881488] [MGI Ref ID J:128348]
Wang N; Yang G; Jia Z; Zhang H; Aoyagi T; Soodvilai S; Symons JD; Schnermann JB; Gonzalez FJ; Litwin SE; Yang T. 2008. Vascular PPARgamma controls circadian variation in blood pressure and heart rate through Bmal1. Cell Metab 8(6):482-91. [PubMed: 19041764] [MGI Ref ID J:144377]
Welsh M; Saunders PT; Atanassova N; Sharpe RM; Smith LB. 2009. Androgen action via testicular peritubular myoid cells is essential for male fertility. FASEB J 23(12):4218-30. [PubMed: 19692648] [MGI Ref ID J:155167]
Weskamp G; Mendelson K; Swendeman S; Le Gall S; Ma Y; Lyman S; Hinoki A; Eguchi S; Guaiquil V; Horiuchi K; Blobel CP. 2010. Pathological neovascularization is reduced by inactivation of ADAM17 in endothelial cells but not in pericytes. Circ Res 106(5):932-40. [PubMed: 20110534] [MGI Ref ID J:170866]
Xin M; Small EM; van Rooij E; Qi X; Richardson JA; Srivastava D; Nakagawa O; Olson EN. 2007. Essential roles of the bHLH transcription factor Hrt2 in repression of atrial gene expression and maintenance of postnatal cardiac function. Proc Natl Acad Sci U S A 104(19):7975-80. [PubMed: 17468400] [MGI Ref ID J:121577]
Zeini M; Hang CT; Lehrer-Graiwer J; Dao T; Zhou B; Chang CP. 2009. Spatial and temporal regulation of coronary vessel formation by calcineurin-NFAT signaling. Development 136(19):3335-45. [PubMed: 19710169] [MGI Ref ID J:153636]
Zhang C; Yeh S; Chen YT; Wu CC; Chuang KH; Lin HY; Wang RS; Chang YJ; Mendis-Handagama C; Hu L; Lardy H; Chang C. 2006. Oligozoospermia with normal fertility in male mice lacking the androgen receptor in testis peritubular myoid cells. Proc Natl Acad Sci U S A 103(47):17718-23. [PubMed: 17095600] [MGI Ref ID J:117118]
Zhou L; Choi HY; Li WP; Xu F; Herz J. 2009. LRP1 controls cPLA2 phosphorylation, ABCA1 expression and cellular cholesterol export. PLoS One 4(8):e6853. [PubMed: 19718435] [MGI Ref ID J:152391]
Zhou L; Takayama Y; Boucher P; Tallquist MD; Herz J. 2009. LRP1 regulates architecture of the vascular wall by controlling PDGFRbeta-dependent phosphatidylinositol 3-kinase activation. PLoS One 4(9):e6922. [PubMed: 19742316] [MGI Ref ID J:153626]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry The strain is maintained as a homozygote on a mixed background of 129S5/SvEvBrd, C57BL6 and SJL. Expected coat color is black and agouti. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Tg(Tagln-cre)1Her
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Tg(Tagln-cre)1Her x Homozygous for Tg(Tagln-cre)1Her Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Tg(Tagln-cre)1Her
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Tg(Tagln-cre)1Her x Homozygous for Tg(Tagln-cre)1Her Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.