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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names STOCK Tg(Tagln-cre)1Her/J (Changed: 15-DEC-04 ) STOCK Tg(Tagln-cre)1Kuhn/J (Changed: 15-DEC-04 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation F?+F9 (31-DEC-07) Donating Investigator Joachim Herz, Univ of Texas Southwest Med Ctr Dallas Description
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse transgelin (smooth muscle protein 22-alpha) promoter. Cre recombinase expression (mRNA) closely patterns endogenous transgelin expression, with the highest levels detected in the aorta, intestine and uterus. Low levels of transcript are detected in all other organs tested, likely reflecting the vascular smooth muscle compartments in the these tissues. Cre recombinase activity is observed in vascular smooth muscle cells of hepatic and pulmonary arteries, with no activity detected outside the vascular walls. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in vascular smooth muscle cells. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful in studies of vascular diseases such as atherosclerosis.Development
A transgenic construct containing cre coding sequence under the control of the mouse transgelin promoter was introduced into B6SJLF2 donor oocytes.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Tagln
006878 B6.129S6-Taglntm2(cre)Yec/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J View Strains carrying other alleles of Tagln (3 strains)
Additional Web Information
Cre-lox Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Tagln-cre)1Her | ||
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| Allele Name | transgene insertion 1, Joachim Herz | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | SM22-Cre; SM22-Cretg; Sm22alphaCre; Tag1-Cre; | ||
| Mutation Made By | Joachim Herz, Univ of Texas Southwest Med Ctr Dallas | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | vascular smooth muscle cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Tagln, transgelin, mouse, laboratory | ||
| General Note | Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. | ||
| Molecular Note | The transgene consists of a Tagln promoter and a cre gene modified to include translational consensus and nuclear localization signals. The Tagln promoter was used to drive transgene expression in smooth muscle cells. Cre recombinase mRNA expression closely patterns endogenous transgelin expression, with the highest levels detected in the aorta, intestine, and uterus. Low levels of Cre recombinase transcript are detected in all other organs. Recombination occurs in vascular smooth muscle cells of hepaticand pulmonary arteries. No recombination has been detected in non-muscle cells. [MGI Ref ID J:76460] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Quantitative PCR, QPCR, vers. 1
Generic Cre, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Boucher P; Gotthardt M; Li WP; Anderson RG; Herz J. 2003. LRP: role in vascular wall integrity and protection from atherosclerosis. Science 300(5617):329-32. [PubMed: 12690199] [MGI Ref ID J:82871]
Holtwick R; Gotthardt M; Skryabin B; Steinmetz M; Potthast R; Zetsche B; Hammer RE; Herz J; Kuhn M. 2002. Smooth muscle-selective deletion of guanylyl cyclase-A prevents the acute but not chronic effects of ANP on blood pressure. Proc Natl Acad Sci U S A 99(10):7142-7. [PubMed: 11997476] [MGI Ref ID J:76460]
Additional References
Tg(Tagln-cre)1Her relatedBoucher P; Li WP; Matz RL; Takayama Y; Auwerx J; Anderson RG; Herz J. 2007. LRP1 functions as an atheroprotective integrator of TGFbeta and PDFG signals in the vascular wall: implications for Marfan syndrome. PLoS ONE 2(5):e448. [PubMed: 17505534] [MGI Ref ID J:129347]
Carvalho RL; Itoh F; Goumans MJ; Lebrin F; Kato M; Takahashi S; Ema M; Itoh S; van Rooijen M; Bertolino P; Ten Dijke P; Mummery CL. 2007. Compensatory signalling induced in the yolk sac vasculature by deletion of TGF receptors in mice. J Cell Sci 120(Pt 24):4269-77. [PubMed: 18029401] [MGI Ref ID J:128498]
El-Bizri N; Guignabert C; Wang L; Cheng A; Stankunas K; Chang CP; Mishina Y; Rabinovitch M. 2008. SM22alpha-targeted deletion of bone morphogenetic protein receptor 1A in mice impairs cardiac and vascular development, and influences organogenesis. Development 135(17):2981-91. [PubMed: 18667463] [MGI Ref ID J:139945]
French WJ; Creemers EE; Tallquist MD. 2008. Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol 28(18):5646-57. [PubMed: 18606782] [MGI Ref ID J:140245]
Frutkin AD; Shi H; Otsuka G; Leveen P; Karlsson S; Dichek DA. 2006. A critical developmental role for tgfbr2 in myogenic cell lineages is revealed in mice expressing SM22-Cre, not SMMHC-Cre. J Mol Cell Cardiol 41(4):724-31. [PubMed: 16887142] [MGI Ref ID J:114624]
Hansmann G; de Jesus Perez VA; Alastalo TP; Alvira CM; Guignabert C; Bekker JM; Schellong S; Urashima T; Wang L; Morrell NW; Rabinovitch M. 2008. An antiproliferative BMP-2/PPARgamma/apoE axis in human and murine SMCs and its role in pulmonary hypertension. J Clin Invest 118(5):1846-57. [PubMed: 18382765] [MGI Ref ID J:136168]
Hernando E; Charytonowicz E; Dudas ME; Menendez S; Matushansky I; Mills J; Socci ND; Behrendt N; Ma L; Maki RG; Pandolfi PP; Cordon-Cardo C. 2007. The AKT-mTOR pathway plays a critical role in the development of leiomyosarcomas. Nat Med 13(6):748-753. [PubMed: 17496901] [MGI Ref ID J:121901]
Nanba D; Kinugasa Y; Morimoto C; Koizumi M; Yamamura H; Takahashi K; Takakura N; Mekada E; Hashimoto K; Higashiyama S. 2006. Loss of HB-EGF in smooth muscle or endothelial cell lineages causes heart malformation. Biochem Biophys Res Commun 350(2):315-21. [PubMed: 17010937] [MGI Ref ID J:116051]
Navankasattusas S; Whitehead KJ; Suli A; Sorensen LK; Lim AH; Zhao J; Park KW; Wythe JD; Thomas KR; Chien CB; Li DY. 2008. The netrin receptor UNC5B promotes angiogenesis in specific vascular beds. Development 135(4):659-67. [PubMed: 18223200] [MGI Ref ID J:131817]
Otsuka G; Stempien-Otero A; Frutkin AD; Dichek DA. 2007. Mechanisms of TGF-beta1-induced intimal growth: plasminogen-independent activities of plasminogen activator inhibitor-1 and heterogeneous origin of intimal cells. Circ Res 100(9):1300-7. [PubMed: 17431190] [MGI Ref ID J:137777]
Sabrane K; Gambaryan S; Brandes RP; Holtwick R; Voss M; Kuhn M. 2003. Increased sensitivity to endothelial nitric oxide (NO) contributes to arterial normotension in mice with vascular smooth muscle-selective deletion of the atrial natriuretic peptide (ANP) receptor. J Biol Chem 278(20):17963-8. [PubMed: 12637561] [MGI Ref ID J:124744]
Stankunas K; Hang CT; Tsun ZY; Chen H; Lee NV; Wu JI; Shang C; Bayle JH; Shou W; Iruela-Arispe ML; Chang CP. 2008. Endocardial Brg1 represses ADAMTS1 to maintain the microenvironment for myocardial morphogenesis. Dev Cell 14(2):298-311. [PubMed: 18267097] [MGI Ref ID J:132583]
Steinmetz M; Potthast R; Sabrane K; Kuhn M. 2004. Diverging vasorelaxing effects of C-type natriuretic peptide in renal resistance arteries and aortas of GC-A-deficient mice. Regul Pept 119(1-2):31-7. [PubMed: 15093694] [MGI Ref ID J:102436]
Umans L; Cox L; Tjwa M; Bito V; Vermeire L; Laperre K; Sipido K; Moons L; Huylebroeck D; Zwijsen A. 2007. Inactivation of Smad5 in Endothelial Cells and Smooth Muscle Cells Demonstrates that Smad5 Is Required for Cardiac Homeostasis. Am J Pathol 170(5):1460-72. [PubMed: 17456754] [MGI Ref ID J:121072]
Varadkar P; Kraman M; Despres D; Ma G; Lozier J; McCright B. 2008. Notch2 is required for the proliferation of cardiac neural crest-derived smooth muscle cells. Dev Dyn 237(4):1144-52. [PubMed: 18330927] [MGI Ref ID J:132939]
Viana R; Batourina E; Huang H; Dressler GR; Kobayashi A; Behringer RR; Shapiro E; Hensle T; Lambert S; Mendelsohn C. 2007. The development of the bladder trigone, the center of the anti-reflux mechanism. Development 134(20):3763-9. [PubMed: 17881488] [MGI Ref ID J:128348]
Xin M; Small EM; van Rooij E; Qi X; Richardson JA; Srivastava D; Nakagawa O; Olson EN. 2007. Essential roles of the bHLH transcription factor Hrt2 in repression of atrial gene expression and maintenance of postnatal cardiac function. Proc Natl Acad Sci U S A 104(19):7975-80. [PubMed: 17468400] [MGI Ref ID J:121577]
Zhang C; Yeh S; Chen YT; Wu CC; Chuang KH; Lin HY; Wang RS; Chang YJ; Mendis-Handagama C; Hu L; Lardy H; Chang C. 2006. Oligozoospermia with normal fertility in male mice lacking the androgen receptor in testis peritubular myoid cells. Proc Natl Acad Sci U S A 103(47):17718-23. [PubMed: 17095600] [MGI Ref ID J:117118]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry The strain is maintained as a homozygote on a mixed background of 129S5/SvEvBrd, C57BL6 and SJL. Expected coat color is black and agouti. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $104.80 Female or Male Homozygous for Tg(Tagln-cre)1Her *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $209.60 Homozygous for Tg(Tagln-cre)1Her x Homozygous for Tg(Tagln-cre)1Her
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $136.30 Female or Male Homozygous for Tg(Tagln-cre)1Her *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $272.50 Homozygous for Tg(Tagln-cre)1Her x Homozygous for Tg(Tagln-cre)1Her
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Terms of Use
Terms of Use
General Terms and Conditions
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
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Contact information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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