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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names STOCK Mapttm1(GFP)Klt/J (Changed: 14-APR-06 ) B6;129S-Mapttm1(EGFP)Klt/J (Changed: 15-DEC-04 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation N1F7 (08-JAN-08) Donating Investigator Kerry Tucker, Ruprecht Karls University of Heidelberg Description
Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. A knock-in of the EGFP coding sequence into the first exon disrupts expression of the Mapt gene and produces a cytoplasmic EGFP fused to the first 31 amino acids. No gene product (isoform proteins) is detected in whole brain lysates by Western blot analysis. EGFP signal is detected beginning at 9.0 days post coitum in the trigeminal ganglion and by 10.75 days post coitum fluorescent signal is detected throughout the developing central nervous system. EGFP expression persists to adult and closely patterns the expression of neuron specific beta-tubulin III, as detected by the TuJ1 antibody. The expression of cytoplasmic EGFP in the central nervous system of this mutant allows for non-invasive visualization of elongating nerve axons.Development
A targeting vector containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter and a EGFP (Clonetech) cDNA was inserted into exon 1. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were crossed to C57BL/6 mice. Heterozygotes were intercrossed to generate homozygotes. The mice were then crossed to a transgenic strain that was generated in embryos derived from a cross between Swiss Webster females and B6D2F1 males. This transgenic allele was subsequently removed from the strain during the development of our production stocks through selective breeding. These mice carry only the Mapttm1(EGFP)Klt allele.
Control Information
| Control | ||
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| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (173 strains)
005491 B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
007251 B6.129-Mapttm1Hnd/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Mapttm1(EGFP)Klt/Mapttm1(EGFP)Klt
involves: 129S4/SvJae * C57BL/6
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:66561)
- viable and fertile with no central or peripheral nervous system defects
This mouse can be used to support research in many areas including:
Mapttm1(EGFP)Klt relatedNeurobiology Research
Alzheimer's Disease
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Neurobiology Research
Alzheimer's Disease (Tau (Mapt) mutants)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Mapttm1(EGFP)Klt | ||
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| Allele Name | targeted mutation 1, Kerry Lee Tucker | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Mapttm1(GFP)Klt; tau ko; | ||
| Mutation Made By | Kerry Tucker, Ruprecht Karls University of Heidelberg | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Site of Expression | GFP signal is detected beginning a embryonic day 9 in the trigeminal ganglion and throughout the developing central nervous system by embryonic day 10.75. GFP expression persists in adults and closely resembles the expression of neuron specific beta-tubulin III. | ||
| Gene Symbol and Name | Mapt, microtubule-associated protein tau | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | AI413597; AW045860; DDPAC; FLJ31424; FTDP-17; MAPTL; MGC138549; MGC156663; MSTD; MTBT1; MTBT2; Mtapt; PPND; RNPTAU; TAU; Tau; expressed sequence AI413597; expressed sequence AW045860; pTau; | ||
| Molecular Note | Exon 1 was disrupted by the insertion of a cassette encoding EGFP and neo. Fusion protein including GFP was detected by Western blot analysis of homozygous mutant brain lysates. Fluorescent illumination indicated that expression of the reporter gene was similar to that of the endogenous gene. [MGI Ref ID J:66561] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Mapttm1(EGFP)Klt, MCA, vers. 3
Mapttm1(EGFP)Klt, STD PCR, vers. 2
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Tucker KL; Meyer M; Barde YA. 2001. Neurotrophins are required for nerve growth during development. Nat Neurosci 4(1):29-37. [PubMed: 11135642] [MGI Ref ID J:66561]
Additional References
Mapttm1(EGFP)Klt relatedAndorfer C; Acker CM; Kress Y; Hof PR; Duff K; Davies P. 2005. Cell-cycle reentry and cell death in transgenic mice expressing nonmutant human tau isoforms. J Neurosci 25(22):5446-54. [PubMed: 15930395] [MGI Ref ID J:98717]
Andorfer C; Kress Y; Espinoza M; de Silva R; Tucker KL; Barde YA; Duff K; Davies P. 2003. Hyperphosphorylation and aggregation of tau in mice expressing normal human tau isoforms. J Neurochem 86(3):582-90. [PubMed: 12859672] [MGI Ref ID J:84638]
Dickey CA; Kamal A; Lundgren K; Klosak N; Bailey RM; Dunmore J; Ash P; Shoraka S; Zlatkovic J; Eckman CB; Patterson C; Dickson DW; Nahman NS Jr; Hutton M; Burrows F; Petrucelli L. 2007. The high-affinity HSP90-CHIP complex recognizes and selectively degrades phosphorylated tau client proteins. J Clin Invest 117(3):648-58. [PubMed: 17304350] [MGI Ref ID J:120752]
Kelleher I; Garwood C; Hanger DP; Anderton BH; Noble W. 2007. Kinase activities increase during the development of tauopathy in htau mice. J Neurochem 103(6):2256-67. [PubMed: 17908241] [MGI Ref ID J:128741]
Yuan A; Kumar A; Peterhoff C; Duff K; Nixon RA. 2008. Axonal transport rates in vivo are unaffected by tau deletion or overexpression in mice. J Neurosci 28(7):1682-7. [PubMed: 18272688] [MGI Ref ID J:132278]
Zaghetto AA; Paina S; Mantero S; Platonova N; Peretto P; Bovetti S; Puche A; Piccolo S; Merlo GR. 2007. Activation of the Wnt-beta catenin pathway in a cell population on the surface of the forebrain is essential for the establishment of olfactory axon connections. J Neurosci 27(36):9757-68. [PubMed: 17804636] [MGI Ref ID J:124891]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice may be bred as homozygotes. Expected coat color is Agouti and Black. Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $155.70 Female or Male Homozygous for Mapttm1(EGFP)Klt *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $311.40 Homozygous for Mapttm1(EGFP)Klt x Homozygous for Mapttm1(EGFP)Klt
Additional Supply Details
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $202.50 Female or Male Homozygous for Mapttm1(EGFP)Klt *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $404.90 Homozygous for Mapttm1(EGFP)Klt x Homozygous for Mapttm1(EGFP)Klt
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
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