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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names STOCK Mapttm1(GFP)Klt/J (Changed: 14-APR-06 ) B6;129S-Mapttm1(EGFP)Klt/J (Changed: 15-DEC-04 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 12-FEB-08 Species laboratory mouse Generation N1F9 (22-JAN-09)
Generation DefinitionsDonating Investigator Kerry Tucker, Ruprecht Karls University of Heidelberg Description
Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. A knock-in of the EGFP coding sequence into the first exon disrupts expression of the Mapt gene and produces a cytoplasmic EGFP fused to the first 31 amino acids. No gene product (isoform proteins) is detected in whole brain lysates by Western blot analysis. EGFP signal is detected beginning at 9.0 days post coitum in the trigeminal ganglion and by 10.75 days post coitum fluorescent signal is detected throughout the developing central nervous system. EGFP expression persists to adult and closely patterns the expression of neuron specific beta-tubulin III, as detected by the TuJ1 antibody. The expression of cytoplasmic EGFP in the central nervous system of this mutant allows for non-invasive visualization of elongating nerve axons.Development
A targeting vector containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter and a EGFP (Clonetech) cDNA was inserted into exon 1. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were crossed to C57BL/6 mice. Heterozygotes were intercrossed to generate homozygotes. The mice were then crossed to a transgenic strain that was generated in embryos derived from a cross between Swiss Webster females and B6D2F1 males. This transgenic allele was subsequently removed from the strain during the development of our production stocks through selective breeding. These mice carry only the Mapttm1(EGFP)Klt allele.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Related Strains
Alzheimer's Disease Models
View Alzheimer's Disease Models (108 strains)
005491 B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
007251 B6.129-Mapttm1Hnd/J 005491 B6.Cg-Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J 012639 B6;129S4-Mapttm3(HDAC2)Jae/J 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Mapttm1(EGFP)Klt/Mapttm1(EGFP)Klt
involves: 129S4/SvJae * C57BL/6
- normal phenotype
- no abnormal phenotype detected
- homozygotes are viable and fertile with no detectable abnormalities in morphology of the central or peripheral nervous systems viable and fertile with no central or peripheral nervous system defects (MGI Ref ID J:66561)
- behavior/neurological phenotype
- *normal* behavior/neurological phenotype
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Alzheimer's Disease
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Mapttm1(EGFP)Klt relatedResearch Tools
Fluorescent Proteins
Neurobiology Research
Alzheimer's Disease
Tau (Mapt) mutants
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Mapttm1(EGFP)Klt | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Kerry Lee Tucker | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Mapttm1(GFP)Klt; tau ko; tauGFP; | ||
| Mutation Made By | Kerry Tucker, Ruprecht Karls University of Heidelberg | ||
| Strain of Origin | 129S4/SvJae | ||
| ES Cell Line Name | J1 | ||
| ES Cell Line Strain | 129S4/SvJae | ||
| Site of Expression | GFP signal is detected beginning a embryonic day 9 in the trigeminal ganglion and throughout the developing central nervous system by embryonic day 10.75. GFP expression persists in adults and closely resembles the expression of neuron specific beta-tubulin III. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Molecular Note | Exon 1 was disrupted by the insertion of a cassette encoding EGFP and neo. Fusion protein including GFP was detected by Western blot analysis of homozygous mutant brain lysates. Fluorescent illumination indicated that expression of the reporter gene was similar to that of the endogenous gene. [MGI Ref ID J:66561] | ||
| Gene Symbol and Name | Mapt, microtubule-associated protein tau | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | AI413597; AW045860; DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; Mtapt; PPND; RNPTAU; TAU; Tau; expressed sequence AI413597; expressed sequence AW045860; pTau; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Mapttm1(EGFP)Klt, Melt Curve Analysis
Mapttm1(EGFP)Klt, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Tucker KL; Meyer M; Barde YA. 2001. Neurotrophins are required for nerve growth during development. Nat Neurosci 4(1):29-37. [PubMed: 11135642] [MGI Ref ID J:66561]
Additional References
Mapttm1(EGFP)Klt relatedAndo K; Leroy K; Heraud C; Yilmaz Z; Authelet M; Suain V; De Decker R; Brion JP. 2011. Accelerated human mutant tau aggregation by knocking out murine tau in a transgenic mouse model. Am J Pathol 178(2):803-16. [PubMed: 21281813] [MGI Ref ID J:169074]
Andorfer C; Acker CM; Kress Y; Hof PR; Duff K; Davies P. 2005. Cell-cycle reentry and cell death in transgenic mice expressing nonmutant human tau isoforms. J Neurosci 25(22):5446-54. [PubMed: 15930395] [MGI Ref ID J:98717]
Andorfer C; Kress Y; Espinoza M; de Silva R; Tucker KL; Barde YA; Duff K; Davies P. 2003. Hyperphosphorylation and aggregation of tau in mice expressing normal human tau isoforms. J Neurochem 86(3):582-90. [PubMed: 12859672] [MGI Ref ID J:84638]
Boutajangout A; Quartermain D; Sigurdsson EM. 2010. Immunotherapy targeting pathological tau prevents cognitive decline in a new tangle mouse model. J Neurosci 30(49):16559-66. [PubMed: 21147995] [MGI Ref ID J:166739]
Dickey CA; Kamal A; Lundgren K; Klosak N; Bailey RM; Dunmore J; Ash P; Shoraka S; Zlatkovic J; Eckman CB; Patterson C; Dickson DW; Nahman NS Jr; Hutton M; Burrows F; Petrucelli L. 2007. The high-affinity HSP90-CHIP complex recognizes and selectively degrades phosphorylated tau client proteins. J Clin Invest 117(3):648-58. [PubMed: 17304350] [MGI Ref ID J:120752]
Ittner LM; Ke YD; Delerue F; Bi M; Gladbach A; van Eersel J; Wolfing H; Chieng BC; Christie MJ; Napier IA; Eckert A; Staufenbiel M; Hardeman E; Gotz J. 2010. Dendritic function of tau mediates amyloid-beta toxicity in Alzheimer's disease mouse models. Cell 142(3):387-97. [PubMed: 20655099] [MGI Ref ID J:164597]
Keimpema E; Barabas K; Morozov YM; Tortoriello G; Torii M; Cameron G; Yanagawa Y; Watanabe M; Mackie K; Harkany T. 2010. Differential subcellular recruitment of monoacylglycerol lipase generates spatial specificity of 2-arachidonoyl glycerol signaling during axonal pathfinding. J Neurosci 30(42):13992-4007. [PubMed: 20962221] [MGI Ref ID J:165476]
Kelleher I; Garwood C; Hanger DP; Anderton BH; Noble W. 2007. Kinase activities increase during the development of tauopathy in htau mice. J Neurochem 103(6):2256-67. [PubMed: 17908241] [MGI Ref ID J:128741]
Kickstein E; Krauss S; Thornhill P; Rutschow D; Zeller R; Sharkey J; Williamson R; Fuchs M; Kohler A; Glossmann H; Schneider R; Sutherland C; Schweiger S. 2010. Biguanide metformin acts on tau phosphorylation via mTOR/protein phosphatase 2A (PP2A) signaling. Proc Natl Acad Sci U S A :. [PubMed: 21098287] [MGI Ref ID J:167148]
Korets-Smith E; Lindemann L; Tucker KL; Jiang C; Kabacs N; Belteki G; Haigh J; Gertsenstein M; Nagy A. 2004. Cre recombinase specificity defined by the tau locus. Genesis 40(3):131-8. [PubMed: 15493019] [MGI Ref ID J:94203]
Levine S; Saltzman A; Levy E; Ginsberg SD. 2009. Systemic pathology in aged mouse models of Down's syndrome and Alzheimer's disease. Exp Mol Pathol 86(1):18-22. [PubMed: 19041304] [MGI Ref ID J:174270]
Pacheco CD; Elrick MJ; Lieberman AP. 2009. Tau deletion exacerbates the phenotype of Niemann-Pick type C mice and implicates autophagy in pathogenesis. Hum Mol Genet 18(5):956-65. [PubMed: 19074461] [MGI Ref ID J:145003]
Phillips M; Boman E; Osterman H; Willhite D; Laska M. 2011. Olfactory and visuospatial learning and memory performance in two strains of Alzheimer's disease model mice-a longitudinal study. PLoS One 6(5):e19567. [PubMed: 21573167] [MGI Ref ID J:172426]
Polydoro M; Acker CM; Duff K; Castillo PE; Davies P. 2009. Age-dependent impairment of cognitive and synaptic function in the htau mouse model of tau pathology. J Neurosci 29(34):10741-9. [PubMed: 19710325] [MGI Ref ID J:152310]
Willaredt MA; Hasenpusch-Theil K; Gardner HA; Kitanovic I; Hirschfeld-Warneken VC; Gojak CP; Gorgas K; Bradford CL; Spatz J; Wolfl S; Theil T; Tucker KL. 2008. A crucial role for primary cilia in cortical morphogenesis. J Neurosci 28(48):12887-900. [PubMed: 19036983] [MGI Ref ID J:142500]
Yuan A; Kumar A; Peterhoff C; Duff K; Nixon RA. 2008. Axonal transport rates in vivo are unaffected by tau deletion or overexpression in mice. J Neurosci 28(7):1682-7. [PubMed: 18272688] [MGI Ref ID J:132278]
Zaghetto AA; Paina S; Mantero S; Platonova N; Peretto P; Bovetti S; Puche A; Piccolo S; Merlo GR. 2007. Activation of the Wnt-beta catenin pathway in a cell population on the surface of the forebrain is essential for the establishment of olfactory axon connections. J Neurosci 27(36):9757-68. [PubMed: 17804636] [MGI Ref ID J:124891]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice may be bred as homozygotes. Expected coat color is Agouti and Black. Mating System Homozygote x Homozygote (Female x Male) 12-FEB-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $172.00 Female or Male Homozygous for Mapttm1(EGFP)Klt
Pairs /Price (US dollars $) Pair Genotype $344.00 Homozygous for Mapttm1(EGFP)Klt x Homozygous for Mapttm1(EGFP)Klt Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $223.60 Female or Male Homozygous for Mapttm1(EGFP)Klt
Pairs /Price (US dollars $) Pair Genotype $447.20 Homozygous for Mapttm1(EGFP)Klt x Homozygous for Mapttm1(EGFP)Klt Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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