Strain Name: |
B6.129P2-Lyz2tm1(cre)Ifo/J |
|---|---|
Stock Number: |
004781 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
B6.129P2-Lyzstm1(cre)Ifo/J (Changed: 19-FEB-08
) |
|
B6.129-Lyzstm1(cre)Ifo/J (Changed: 15-DEC-04
) | |
| Genes & Alleles | Lyz2; Lyz2tm1(cre)Ifo; cre; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Irmgard Foerster, University of Duesseldorf Generation N6F?+F9 (19-DEC-07) Strain Description
This strain expresses Cre recombinase from the endogenous Lyzs locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in deletion of the targeted gene in the myeloid cell lineage, including monocytes, mature macrophages, and granulocytes. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain represents an effective tool for generating myeloid cell-specific targeted mutants.Strain Development
A targeting vector containing a FRT-flanked neomycin resistance gene, herpes simplex virus thymidine kinase genes and NLS (nuclear localization signal) -Cre cDNA, was inserted into the endogenous ATG-start site within exon 1 of the Lyzs gene. The construct was electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Flp expression vector for the purpose of removing the selectable marker cassette. ES cells that had successfully undergone Flp recombination were injected in blastocysts.
Gene & Allele Details
| Allele Symbol | Lyz2tm1(cre)Ifo | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Irmgard Foerster | ||
| Common Name(s) | LC; Lys-cre; LysM Cre; LysM-Cre; LysMCre; LysMCre; lysM/cre; | ||
| Mutation Made By | Irmgard Foerster, University of Duesseldorf | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | myeloid cells - including monocytes, mature macrophages, and granulocytes | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Lyz2, lysozyme 2 | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | AI326280; Lys; Lysz; Lyz; Lyzs; Lzm; Lzm-s1; Lzp; M lysozyme structural 1; expressed sequence AI326280; lysozyme; | ||
| Molecular Note | An NLS-cre gene was inserted into the endogenous ATG start site of the gene. An frt-flanked neomycin cassette was removed in ES cells via Flp-mediated recombination prior to production of chimeric mice. [MGI Ref ID J:67924] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Lyz2tm1(cre)Ifo
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (115 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedHematological Research
Hematopoietic Defects
Immunology and Inflammation Research
Autoimmunity
Immunodeficiency
Lymphoid Tissue Defects (hematopoietic development)
Internal/Organ Research
Lymphoid Tissue Defects
Research Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Tissue/Cell Markers: Cre-lox System)
Immunology and Inflammation Research (Macrophage Deficiency)
Lyz2tm1(cre)Ifo relatedResearch Tools
Cre-lox System
Developmental Biology Research
Internal/Organ Defects (Lymphoid Tissue Defects)
Lymphoid Tissue Defects (hematopoietic defects)
Hematological Research
Hematopoietic Defects
Immunological Defects
Neutrophil Defects
Immunology and Inflammation Research
Autoimmunity (experimentally induced rheumatoid arthritis)
CD Antigens, Antigen Receptors, and Histocompatibility Markers (genes regulating susceptibility to infectious disease and endotoxin)
Growth Factors/Receptors/Cytokines
Immunodeficiency (Neutrophil Defects)
Inflammation (Neutrophil defects)
Lymphoid Tissue Defects (hematopoietic development)
Lymphoid Tissue Defects
Internal/Organ Research
Gastrointestinal Defects
Lymphoid Tissue Defects
Research Tools
Cancer Research (Leukemia)
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Hematological Research
Immunology and Inflammation Research (Macrophage Deficiency)
Immunology and Inflammation Research (genes regulating susceptibility to infectious disease and endotoxin)
References
Selected Reference(s)
Additional ReferencesClausen BE; Burkhardt C; Reith W; Renkawitz R; Forster I. 1999. Conditional gene targeting in macrophages and granulocytes using LysMcre mice. Transgenic Res 8(4):265-77. [PubMed: 10621974] [MGI Ref ID J:67924]
Price and Supply Information
| Strain Name: | B6.129P2-Lyz2tm1(cre)Ifo/J |
| Stock Number: | 004781 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $104.80 | Homozygous for Lyz2tm1(cre)Ifo | |||
| Pair | $209.60 | Homozygous for Lyz2tm1(cre)Ifo x Homozygous for Lyz2tm1(cre)Ifo | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
- Use of MICE by companies or for-profit entities requires a license.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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